27,462 results
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Plasmid#112264PurposeITRACKalpha Donor ConstructDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertprotein kinase C alpha (PRKCA Bovine)
TagsmEGFPExpressionMammalianMutationA11V (please see depositor comments below)PromoterCMVAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV-mCh-kCAAX
Plasmid#112267PurposeITRACK Acceptor ConstructDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmCherry with KRas membrane targeting domain
TagsmCherryExpressionMammalianPromoterCMVAvailable sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-puro-eGFP-HEC1
Plasmid#114022PurposeInducible expression of eGFP-tagged HEC1 in FLPin Trex cell linesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserteGFP-HEC1 - Wildtype (NDC80) (NDC80 Human)
ExpressionMammalianAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-S1P1-GFP11-T2A-GFP10-bArrestin1-T2A mCherry
Plasmid#113612PurposePPI reporterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertS1P1
ExpressionMammalianAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTfR-mScarlet-I
Plasmid#112954PurposeIn vivo visualization of Transferrin receptors (can be used for colocalization studies)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertpTfR
TagsmScarlet-IExpressionMammalianPromoterCMVAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Venus-173-N-ORAI1
Plasmid#87618PurposeN-terminal Venus (YFP) fragment fused to full-length ORAI1; for BiFC.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertVenus173-N-ORAI1 (ORAI1 Synthetic, Human)
Tags6xHis, T7 tag (gene 10 leader), and Xpress(TM) ta…ExpressionMammalianPromoterCMVAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pST1374-GCN4-D10A
Plasmid#113022PurposeExpresses GCN4-D10A in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGCN4-D10A (GCN4 S. pyogenes, Budding Yeast)
ExpressionMammalianMutationD10APromoterCMVAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CLTA-GFP HDRT Source (pTR 153)
Plasmid#112016PurposeDNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with GFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCLTA-GFP HDRT (CLTA Human)
UseCRISPR and Synthetic BiologyAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEN35-CDKN2A-Ex2-R
Plasmid#110737PurposeEncodes Cas9 nickase and gRNA targeting CDKN2A Exon2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCDKN2A gRNA (CDKN2A Human)
UseCRISPRExpressionMammalianAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEN35-CDKN2A-Ex2-L
Plasmid#110736PurposeEncodes Cas9 nickase and gRNA targeting CDKN2A Exon2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCDKN2A gRNA (CDKN2A Human)
UseCRISPRExpressionMammalianAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHDR-CDKN2A-Ex2-CMV-EGFP
Plasmid#110734PurposeRecombination template for replacing CDKN2A Exon2 with CMV-EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEGFP
UseOtherAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSKC11 - UTRQ
Plasmid#110490PurposeMonitor the expression of Luciferase driven by the NRAS 5'-UTR.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNRAS 5'-UTR (bp 1-254) (NRAS Human)
UseLuciferaseTagsFirefly luciferasePromoterT7Available sinceJuly 20, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
tCRMod-GGSGGS5
Plasmid#111760PurposeExpresses soluble Clover-mRuby2 tension sensor module with 5-repeat GGSGGS extensible domainDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertClover-GGSGGS5-mRuby2 fusion
ExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCI syn iGluu
Plasmid#106122PurposeFast indicator for synaptic glutamate imaging (synapsin promoter)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertiGluSnFR S72T variant
TagsMyc and PDGFRExpressionMammalianMutationChanged Ser 72 to ThrPromotersynapsinAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
EGFP-TRAF2
Plasmid#111202Purposefluorescent fusion proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTRAF2 (TRAF2 Human)
TagsEGFPExpressionMammalianAvailable sinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-N2cP
Plasmid#100808Purposeexpress rabies virus CVS N2c strain phosphoprotein gene (N2cP)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertrabies virus CVS N2c strain phosphoprotein gene
ExpressionMammalianPromoterCAGAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
hCXCR4-LSSmOrange
Plasmid#110197PurposeEncodes for human CXCR4 coding sequence tagged with the LSS-mOrange FPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthCXCR4 (CXCR4 Human)
TagsLSSmOrangeExpressionMammalianPromoterCMVAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEM705 Gsz
Plasmid#109355PurposeGsX chimera with Gαz C-terminusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGsz chimera
TagsNoneExpressionMammalianPromoterCAGAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEXP(V5-APEX2-NES)
Plasmid#107596PurposeExpresses V5-APEX2-NES in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAPEX2-NES
UseFrt site to insert into hek293 t-rex genomeTagsNES and V5ExpressionMammalianPromoterCMV Dox inducibleAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
eMscL WT
Plasmid#107454PurposeeMscL WT is optimized for mammalian rodent neuronal expression to the plasma membrane through a neuron-specific promoter and a voltage-gated channel targeting motifDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertbacterial mechanosensitive ion channel of large conductance (mscL Escherichia Coli)
UseAdeno-associated viralTagsKir2.1 ER export signal (FCYENEV) and tdTomato fl…ExpressionMammalianPromoterhuman synapsin 1Available sinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by