Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Clear All Filters
50 µg Transfection-Ready DNA Clear filter

Applied Filters

Clear All Filters
50 µg Transfection-Ready DNA Clear filter

27,462 results

Showing: 10581 - 10600 of 27462 results

  3. pCMV-monSTIM1

    Plasmid
    #161795
    Purpose
    Mammalian expression plasmid of monSTIM1 (ultra-photosensitive optogenetically-activated STIM1 protein)
    Depositor
    Won Do Heo
    Publication
    Kim et al Nat Commun. 2020 Jan 10;11(1):210. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MonSTIM1 (STIM1 Human, Mustard Weed)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    161795-plasmid-map-sequence-id-369139

    Enlarge

  5. σ3-mCherry

    Plasmid
    #186580
    Purpose
    Expression of adaptor protein 3 (AP3) sigma subunit AP3S1 tagged with mCherry
    Depositor
    Stephen Royle
    Publication
    Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AP3S1 (AP3S1 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    186580-plasmid-map-sequence-id-368433

    Enlarge

  6. pCMV-T7-ABE8.20m-nSpG-P2A-EGFP (KAC1160)

    Plasmid
    #185916
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ABE8.20m-nSpG-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185916-plasmid-map-sequence-id-367354

    Enlarge

  9. pCAGGS SARS-CoV-2 D614G Spike

    Plasmid
    #185692
    Purpose
    Expresses codon-optimized full length SARS-CoV-2 D614G Spike
    Depositor
    Marceline Côté
    Publication
    Benlarbi et al iScience. 2022 Nov 18;25(11):105316. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 D614G Spike (S )
    Expression
    Mammalian
    Mutation
    Contains the following mutation: D614G
    Promoter
    chicken β-actin promoter, CMV enhancer
    Available since
    June 8, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    185692-plasmid-map-sequence-id-363818

    Enlarge

  11. pCAT100

    Plasmid
    #180512
    Purpose
    Plasmid expressing mammalian codon optimized engineered chimeric PlmCasX-R1, sgRNAv2 scaffold, and restriction sites to clone in new spacers
    Depositor
    Jennifer Doudna
    Publication
    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    CasX sgRNAv2
    PlmCasX with DpbCasX R1 loop
    Use
    CRISPR
    Tags
    2x FLAG and SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and U6
    Available since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180512-plasmid-map-sequence-id-359730

    Enlarge

  19. pAT15516_BEAR-GFP-2in1

    Plasmid
    #174082
    Purpose
    BEAR-GFP plasmid with a gRNA that targets its own plasmid
    Depositor
    Ervin Welker
    Publication
    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP split with an intron between amino acids 95-96
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    disrupted 5' splice site
    Promoter
    CMV
    Available since
    Dec. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    174082-plasmid-map-sequence-id-350382

    Enlarge

  20. CoV2-Spike-D614G-Gamma

    Plasmid
    #177963
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Gamma: L13F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F)
    Depositor
    Melanie Ott
    Publication
    Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 Spike protein (Gamma: L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F) (S )
    Expression
    Mammalian
    Mutation
    L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N50…
    Available since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    177963-plasmid-map-sequence-id-348258

    Enlarge

Showing: 10581 - 10600 of 27462 results