Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Clear All Filters
50 µg Transfection-Ready DNA Clear filter

Applied Filters

Clear All Filters
50 µg Transfection-Ready DNA Clear filter

30,881 results

Showing: 10961 - 10980 of 30881 results

  1. pFD115

    Plasmid
    #124768
    Purpose
    pFD115 carries cas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector
    Depositor
    David Bikard
    Publication
    Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Ptet
    cas9
    term PpflB sgRNA (BsaI)
    Use
    CRISPR
    Promoter
    Ptet
    Available since
    Aug. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    124768-plasmid-map-sequence-id-246185

    Enlarge

  7. mCh-KIF1A*-strep

    Plasmid
    #120165
    Purpose
    Expresses a chimera of mCherry (fluorescent tag), a KIF1A-based anterograde motor and streptavidin
    Depositor
    Juan Bonifacino
    Publication
    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    kinesin family member 1A (Kif1a Mouse)
    Tags
    Streptavidin and mCherry
    Expression
    Mammalian
    Mutation
    Truncated KIF1A: 1-405 aa
    Promoter
    CMV
    Available since
    July 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    120165-plasmid-map-sequence-id-251908

    Enlarge

  11. pUF1-dCas9-Sir2a

    Plasmid
    #122510
    Purpose
    Combining with the specific sgRNA sequence to repress the Plasmodium falciparum endogenous gene transcription, through the decreasing of H3K9 acetylation.
    Depositor
    Lubin Jiang
    Publication
    Xiao et al Proc Natl Acad Sci U S A. 2019 Jan 2;116
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-Sir2a
    Use
    CRISPR
    Tags
    3*FLAG
    Mutation
    dCas9(D10A,H840A)
    Promoter
    PfHsp86
    Available since
    June 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    122510-plasmid-map-sequence-id-243592

    Enlarge

  12. pDmelOR-mRho.V5.mER.hOr56a

    Plasmid
    #126479
    Purpose
    Human codon-optimized D. melanogaster Orco (hOrco) and Or56a (hOr56a). Or56a has N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved trafficking in mammalian cells
    Depositor
    Bill Hansson
    Publication
    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Or56a (Or56a Fly)
    Orco (Orco Fly)
    Tags
    H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…
    Expression
    Mammalian
    Mutation
    codon optimization for H. sapiens and codon optim…
    Promoter
    CMV
    Available since
    June 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    126479-plasmid-map-sequence-id-246521

    Enlarge

  14. pcDNA3.1-CD40L (lose of function mutant)-mgSrtA

    Plasmid
    #125796
    Purpose
    for co-expression of a loss of function mutant of CD40L and mgSrtA on cell membrane. A negative control to plasmid pcDNA3.1-CD40L-mgSrtA.
    Depositor
    P. Chen
    Publication
    Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CD40L (lose of function mutant)-mgSrtA
    Expression
    Mammalian
    Available since
    May 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    125796-plasmid-map-sequence-id-245284

    Enlarge

  17. pcDNA3-ExRai-CKAR(T/A)

    Plasmid
    #118410
    Purpose
    Negative-control mutant for ExRai-CKAR biosensor.
    Depositor
    Jin Zhang
    Publication
    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ExRai-CKAR
    Tags
    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…
    Expression
    Mammalian
    Mutation
    Contains Thr-to-Ala mutation in substrate sequenc…
    Promoter
    CMV
    Available since
    April 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    118410-plasmid-map-sequence-id-243088

    Enlarge

  20. Str-KDEL_SBP-mCherry-WNT3A

    Plasmid
    #108345
    Purpose
    synchronize trafficking of WNT3A from the ER (RUSH System)
    Depositor
    David Virshup
    Publication
    Moti et al PLoS One. 2019 Feb 22;14(2):e0212711. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    WNT3A, Wnt family member 3A (WNT3A Human)
    Tags
    IL-2 Signal Sequence and Streptavidin Binding Pro…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    108345-plasmid-map-sequence-id-233770

    Enlarge

Showing: 10961 - 10980 of 30881 results