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27,462 results

Showing: 11781 - 11800 of 27462 results

  2. plk1 T210A

    Plasmid
    #132965
    Purpose
    To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage
    Depositor
    Michael Yaffe
    Publication
    Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    polo-like kinase 1 T210A (PLK1 Human)
    Expression
    Mammalian
    Mutation
    T210A
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    132965-plasmid-map-sequence-id-265935

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  4. pcDNA3.1-3xhfYFP

    Plasmid
    #186544
    Purpose
    Hyperfolder YFP, triple fusion: cytosolic expression in mammalian cells
    Depositor
    Gregory Petsko
    Publication
    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Hyperfolder YFP (3X)
    Expression
    Mammalian
    Mutation
    mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…
    Promoter
    CMV
    Available since
    Nov. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    186544-plasmid-map-sequence-id-368234

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  5. ER2-mhYFP

    Plasmid
    #186528
    Purpose
    Localization and retention of monomeric hyperfolder YFP fluorescent protein in the ER; improved targeting efficiency
    Depositor
    Gregory Petsko
    Publication
    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Monomeric hyperfolder YFP
    Tags
    Calreticulin and KDEL
    Expression
    Mammalian
    Mutation
    hfYFP-S147P/L195M/V206K
    Promoter
    CMV
    Available since
    Nov. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    186528-plasmid-map-sequence-id-373452

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  6. LifeAct-LSSmGFP

    Plasmid
    #186534
    Purpose
    Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to actin
    Depositor
    Gregory Petsko
    Publication
    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LSSmGFP
    Tags
    LifeAct
    Expression
    Mammalian
    Mutation
    hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K
    Promoter
    CMV
    Available since
    Nov. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    186534-plasmid-map-sequence-id-368226

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  7. Tubulin-hfYFP

    Plasmid
    #186515
    Purpose
    Localization of hyperfolder YFP fluorescent protein to tubulin
    Depositor
    Gregory Petsko
    Publication
    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Hyperfolder YFP
    Tags
    Tubulin
    Expression
    Mammalian
    Mutation
    mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…
    Promoter
    CMV
    Available since
    Nov. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    186515-plasmid-map-sequence-id-368091

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  9. pLenti-PGK-EGFP-ALDH1A2-Neo

    Plasmid
    #189743
    Purpose
    The construct was used to express GFP tagged human ALDH1A2 in mammalian cells for the analysis of the subcellular location of this protein.
    Depositor
    Robert Sobol
    Publication
    Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ALDH1A2 (ALDH1A2 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    PGK
    Available since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    189743-plasmid-map-sequence-id-374852

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  10. pBS L30 mRuby3-Rab18

    Plasmid
    #166431
    Purpose
    Rab18 fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.
    Depositor
    Raphael Gaudin
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Rab18 (RAB18 Human)
    Tags
    mRuby3
    Expression
    Mammalian
    Promoter
    L30 weak promoter
    Available since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    166431-plasmid-map-sequence-id-333545

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  11. pJEC819

    Plasmid
    #190815
    Purpose
    CRISPRa backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.
    Depositor
    James Chappell
    Publication
    Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-XTEN-rpoA
    Use
    CRISPR and Synthetic Biology
    Tags
    XTEN (linker), followed by the N-terminal domain …
    Promoter
    SP30
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    190815-plasmid-map-sequence-id-373717

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  14. pSBbi_RP_MicroID2-FLAG-NES

    Plasmid
    #188525
    Purpose
    Encodes the biotin proximity labeling enzyme MicroID2 with a nuclear export sequence (NES) for expression and stable integration into mammalian cells
    Depositor
    James Londino
    Publication
    Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MicroID2
    Use
    Transposon mediated gene integration
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available since
    Sept. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    188525-plasmid-map-sequence-id-372837

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  15. pSBbi_RP_3XNLS-MicroID2-FLAG

    Plasmid
    #188523
    Purpose
    Encodes the biotin proximity labeling enzyme MicroID2 with a 3X nuclear localization sequence (NLS) for expression and stable integration into mammalian cells
    Depositor
    James Londino
    Publication
    Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3XNLS-MicroID2
    Use
    Transposon mediated gene integration
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available since
    Sept. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    188523-plasmid-map-sequence-id-372834

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  16. pCMV-SUN1 mScarlet

    Plasmid
    #187683
    Purpose
    In vivo visualization of SUN1
    Depositor
    Mary Beckerle
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SUN1 (Sun1 Mouse)
    Tags
    mScarlet
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    CMV
    Available since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    187683-plasmid-map-sequence-id-371441

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Showing: 11781 - 11800 of 27462 results