31,422 results

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env8 selectivity (b')

Plasmid

#99837

Purpose

mNeonGreen reporter that places expression of a fluorescent protein reporter (mNeonGreen) under control of the env8HyCbl riboswitch.

Depositor

Robert Batey

Article

Polaski et al J Biol Chem. 2017 May 8. pii: jbc.M117.7

Insert

env8 selectivity (b')

Tags

mNeonGreen

Expression

Bacterial

Mutation

A20G, A68G, G-C pairs in P1 and P3

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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C331

Plasmid

#141123

Purpose

RhlI expressing plasmid

Depositor

Matthew Bennett

Article

Alnahhas et al Nat Commun. 2020 Jul 21;11(1):3659. doi:

Insert

RhlI (rhlI )

Tags

ssrA degradation tag

Expression

Bacterial

Promoter

engineered lac promoter

Available Since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pExp-PpiB

Plasmid

#112572

Purpose

Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and PpiB (peptidyl-prolyl cis-trans isomerase B from E. coli) fusions with optional C-terminal StrepII-tag

Depositor

Marko Hyvönen

Type

Empty backbone

Tags

8xHis-PpiB and Strep tag

Expression

Bacterial

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pRO16Sh2

Plasmid

#180604

Purpose

Expression of ShCAST under control of Plac with pRO1600 origin.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

ShTnsB, ShTnsC, ShTniQ, ShCas12k

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLac

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pSG81-pLas-tetO-deGFPssrA

Plasmid

#102454

Purpose

Expresses deGFP fluorescent protein with ssrA degradation tag in E. coli. The expression is controlled by a pLas-tetO combinatorial promoter.

Depositor

Richard Murray

Article

Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

Insert

deGFP

Tags

ssrA

Expression

Bacterial

Promoter

pLas-tetO

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pJUMP19-Pac

Plasmid

#126957

Purpose

Promoter acceptor vector. Minimal cargo vector; with eGFP without promoter in downstream secondary site. Allows introducing any part using BsmBI.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pHCKan-INSyibp-Nef

Plasmid

#134593

Purpose

Low Phosphate inducible Nef

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

HIV Nef protein

Tags

His tag

Expression

Bacterial

Promoter

yibD

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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GST-Cav FL (1-178)

Plasmid

#22445

Depositor

William Sessa

Article

Garcia-Cardena et al J Biol Chem. 1997 Oct 10. 272(41):25437-

Insert

Caveolin1(1-178)

Tags

GST

Expression

Bacterial

Available Since

Dec. 8, 2009

Availability

Academic Institutions and Nonprofits only

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pFAB815

Plasmid

#47854

Purpose

BIOFAB reporter plasmid for measuring rnpB T1 termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

rnpB T1 terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLTetO9

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-3xMyc

Plasmid

#129569

Purpose

Empty backbone for Myc Tag

Depositor

Kevin Janes

Article

Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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