30,260 results

1. pET-21a(+)/MBP-His-PINK1

   Plasmid

   #38005

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   PINK1 (PINK1 Human)
   

   Use

   Tags

   6xHIS and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 10, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pRSET.HaloTag.v7.AviTag

   Plasmid

   #195486

   Purpose

   Bacterial expression plasmid with Halotag v7 insert plus a C-terminus appended AviTag for enzymatic biotinylation.

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Insert

   HaloTag.v7.AviTag
   

   Use

   Tags

   6xHIS and AviTag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAKlux2

   Plasmid

   #14080

   Depositor

   Attila Karsi

   Article

   Karsi et al Plasmid. 2007 May;57(3):286-95. doi: 10.

   Insert

   Bacterial luciferase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Lac, T3

   Available since

   March 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSFDuet-sumo-h-cGASCD(K427E/K428E)

   Plasmid

   #127162

   Purpose

   expression of truncated human cGAS protein

   Depositor

   Thomas Tuschl

   Article

   Lama et al Nat Commun. 2019 May 21;10(1):2261. doi:

   Insert

   MB21D1 (CGAS Human)
   

   Use

   Tags

   His-SUMO tag

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJYS1Ptac

   Plasmid

   #85545

   Purpose

   Constitutive trancription of FnCpf1 and recT in C.glutamitum, tac promoter

   Depositor

   Sheng Yang

   Article

   Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

   Inserts

   Cpf1
   recT
   

   Use

   CRISPR; Shuttle vector corynebacterium glutamicum…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac and unknown

   Available since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMan operon

   Plasmid

   #124888

   Purpose

   Expresses proteins encoding Man operon in E. faecalis

   Depositor

   Kazuhisa Sekimizu

   Article

   Matsumoto et al Commun Biol. 2019 May 2;2:157. doi: 10.1

   Insert

   manX1, manX2, manY, manZ, manO, EF0025
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSETA_ionRD

   Plasmid

   #172924

   Purpose

   Ionic strength FRET sensor with mCerulean3 and mCitrine for E. coli expression.

   Depositor

   Arnold Boersma

   Article

   Liu et al ACS Chem Biol. 2017 Oct 20;12(10):2510-2

   Insert

   IonicStrengthSensorRD
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pATetO 6XHis-MMLV RT

   Plasmid

   #178797

   Purpose

   Inducible expression of MMLV RT in E. coli

   Depositor

   Andrew Ellington

   Article

   Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

   Insert

   MMLV RT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1.Gαi1-LgB91

   Plasmid

   #134342

   Purpose

   Nanoluc complementation assay. Expression of Gαi1 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi1. Addition of the HA epitope at N terminus of Gαi1.

   Depositor

   Julien Hanson

   Article

   Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

   Insert

   Gαi1-LgB91 (GNAI1 Human)
   

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   T7

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGSKU

    Plasmid

    #72243

    Purpose

    Contains the CORE cassette with convergent KlURA3 and KanMX4 markers and the I-SceI gene under the inducible GAL1 promoter

    Depositor

    Francesca Storici, Michael Resnick

    Article

    Storici et al Proc Natl Acad Sci U S A. 2003 Dec 9;100

    Inserts

    KlURA3
    kanMX4
    I-SceI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. Tn7 integration plasmid

    Plasmid

    #154134

    Purpose

    Suicidal integration plasmid with the Tn7 arms, streptomycin and kanamycin resistance genes and the restriction site to clone the barcode-carrying cassette.

    Depositor

    Shimon Bershtein

    Article

    Jasinska et al Nat Ecol Evol. 2020 Feb 24. pii: 10.1038

    Inserts

    left and right end of Tn7 arm
    Spectinomycin resistant gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Spectinomycin resistance gene promoter and no

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMV306G13+Lux

    Plasmid

    #26160

    Depositor

    Brian Robertson, Siouxsie Wiles

    Article

    Andreu et al PLoS One. 2010 May 24;5(5):e10777.

    Insert

    G13 promoter + Bacterial luciferase operon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    It contains a gram-positive enhanced translation …

    Promoter

    Available since

    Feb. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. mt POLRMT

    Plasmid

    #205059

    Purpose

    POLRMT protein expression

    Depositor

    Smita Patel

    Article

    Ramachandran et al Nucleic Acids Res. 2017 Jan 25;45(2):861

    Insert

    POLRMT (POLRMT Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized

    Promoter

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pE-SUMO CRYGS

    Plasmid

    #80753

    Purpose

    Expression of human gamma S-crystallin in E. coli

    Depositor

    Larry David

    Insert

    CRYGS (CRYGS Human)
    

    Use

    Tags

    6xHIS and Smt3 SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTCF156 HLA-A*02:01

    Plasmid

    #180456

    Purpose

    E. coli expression of soluble MHC protein

    Depositor

    John Altman

    Insert

    HLA-A*02:01
    

    Use

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    Replacement of transmembrane domain with enzymati…

    Promoter

    Available since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTriEx-mCherry-LOV2

    Plasmid

    #81041

    Purpose

    Mammalian or insect expression for wt LOV2

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    LOV2
    

    Use

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV, p10

    Available since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBad-HPII

    Plasmid

    #105839

    Purpose

    expresses E. coli HPII catalase with N-terminal 6x his tag

    Depositor

    Ryan Mehl

    Article

    Mehl et al 2020

    Insert

    hydroperoxidase II catalase
    

    Use

    Tags

    6x his tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBad/arabinose

    Available since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSL-MS2-6X

    Plasmid

    #27118

    Depositor

    Robert Singer

    Article

    Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

    Insert

    MS2 binding sites (6)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUCBB-eGFP

    Plasmid

    #32548

    Depositor

    Claudia Schmidt-dannert

    Article

    Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

    Insert

    eGFP (eGFP )
    

    Use

    Synthetic Biology

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Consitutive lac

    Available since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a_MBP_RBFOX2

    Plasmid

    #224292

    Purpose

    MBP-RBFOX2 protein purification from bacteria

    Depositor

    Chuan He

    Article

    Dou et al Nat Cell Biol. 2023 Sep;25(9):1359-1368.

    Insert

    RNA binding fox-1 homolog 2 (RBFOX2 Human)
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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