31,059 results

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pTRC-APEX2

Plasmid

#72558

Purpose

Expression of cytosolic APEX2 in bacteria

Depositor

Alice Ting

Article

Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

Insert

APEX2

Tags

His6

Expression

Bacterial

Mutation

A134P relative to APEX

Available Since

May 2, 2016

Availability

Academic Institutions and Nonprofits only

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pC0068 PspCas13 (B12) His6-TwinStrep-SUMO-BsaI

Plasmid

#115219

Purpose

Expresses PspCas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

PspCas13

Tags

SUMO

Expression

Bacterial

Available Since

Sept. 21, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pET His10 TEV LIC cloning vector (2B-T-10)

Plasmid

#78173

Depositor

Scott Gradia

Type

Empty backbone

Tags

10xHis and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only

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SRM

Plasmid

#25490

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

SRM (SRM Human)

Tags

His

Expression

Bacterial

Available Since

Aug. 23, 2010

Availability

Industry, Academic Institutions, and Nonprofits
GST-Cdk5

Plasmid

#24894

Depositor

Harish Pant

Article

Amin et al J Neurosci Res. 2002 Feb 1. 67(3):354-62

Insert

CDK5 (CDK5 Human)

Tags

GST

Expression

Bacterial

Available Since

May 17, 2010

Availability

Academic Institutions and Nonprofits only
pSW002

Plasmid

#111248

Purpose

Broad host-range bacterial vector

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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pJK225

Plasmid

#71124

Purpose

Expression of Acetobacter aceti 1023 citrate synthase (AaCSH6) in E. coli

Depositor

T. J. Kappock

Article

Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

Insert

citrate synthase

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Jan. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pBBR1_DVK_AE(J23100-BCD2-E0040m-B0015)

Plasmid

#197660

Purpose

Expresses GFP under the control of J23100 and BCD2

Depositor

Eric Young

Article

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLysscys255

Plasmid

#36429

Depositor

Emine Gunhan, Pete Lollar

Article

Gunhan et al Protein Expr Purif. 2008 Apr;58(2):203-9

Insert

Saporin-3/cys255

Expression

Bacterial

Mutation

cys added at position 255

Promoter

T7

Available Since

May 10, 2012

Availability

Academic Institutions and Nonprofits only

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pBBR2k-GFPuv

Plasmid

#106383

Purpose

Anhydrotetracycline-inducible tet promoter expressing gfpuv on BBR1 origin

Depositor

Brian Pfleger

Article

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Insert

GFPuv

Expression

Bacterial

Promoter

tet

Available Since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

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pHYRSF53

Plasmid

#64696

Purpose

His-Smt3-NpuDnaEN102-CBD

Depositor

Hideo Iwai

Article

Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

Type

Empty backbone

Tags

CBD, HisTag, and NpuDnaE-N102

Expression

Bacterial

Promoter

T7

Available Since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-GFP-CD63

Plasmid

#194989

Purpose

overexpress CD63

Depositor

Angelique Bordey

Insert

GFP-CD63

Expression

Bacterial

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1.Gαi3-LgB91

Plasmid

#134359

Purpose

Nanoluc complementation assay. Expression of Gαi3 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi3. Addition of the HA epitope at N terminus of Gαi3.

Depositor

Julien Hanson

Article

Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

Insert

Gαi3-LgB91 (GNAI3 Human)

Tags

HA

Expression

Bacterial and Mammalian

Promoter

T7

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pMB1_MmPylRS_MmtRNA-Pyl-opt(UGA)

Plasmid

#174515

Purpose

Recoded single aaRS/tRNA plasmid with MmPylRS and MmtRNA-Pyl-opt with the ASL from the serT gene (UGA anticodon)

Depositor

Jason W Chin

Article

Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

Inserts

Mm PylS
MmtRNA-Pyl-opt(UGA)

Expression

Bacterial

Mutation

ASL from the serT gene (UGA anticodon) and recoded

Promoter

GlnS and lpp

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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H6GB1tevP53SS(1-393)

Plasmid

#200311

Purpose

Bacterial expression of human superstable p53 as His6GB1tev fusion

Depositor

Peter Wright

Article

Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

Insert

p53 (TP53 Human)

Tags

His6-GB1-tev

Expression

Bacterial

Mutation

M133L, V203A, N239Y, N268D

Promoter

T7

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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KARS_pNIC-Bio3

Plasmid

#153051

Purpose

Expression construct for producing Avi tagged Lysyl-tRNA synthetase

Depositor

Susanne Gräslund

Insert

KARS (KARS1 Human)

Tags

Avi and His6-TEV

Expression

Bacterial

Promoter

T7

Available Since

June 29, 2020

Availability

Academic Institutions and Nonprofits only

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pK27Sumo_His-SUMO-PLpro (nsp3) (SARS-CoV-2)

Plasmid

#169193

Purpose

To express SARS-CoV-2 Papain-like protease in E. coli

Depositor

John Diffley

Article

Lim et al Biochem J. 2021 Jul 16;478(13):2517-2531

Insert

14His-SUMO-nsp3_E746-K1060 (pp1ab_E1564-K1878) (ORF1ab Synthetic, SARS-CoV-2)

Tags

14His-SUMO

Expression

Bacterial

Mutation

Codon optimised for E. coli

Promoter

T5

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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His-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star)

Plasmid

#222991

Purpose

Bacterial expression of iGeoCas9(C2) construct

Depositor

Jennifer Doudna

Article

Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

Insert

His-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star)

Use

CRISPR

Tags

CL7, His, and MBP

Expression

Bacterial

Promoter

tac

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pBbS8k-RFP_R43W_E93G

Plasmid

#109406

Purpose

RepA Mutant

Depositor

Jay Keasling

Article

Thompson et al Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.

Insert

repA

Tags

RFP

Expression

Bacterial

Mutation

R43W and E93G repA mutations to increase copy num…

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-nanoHER2-SpyTag003

Plasmid

#216312

Purpose

Expresses nanoHER2-SpyTag003 in bacterial cells. nanoHER2 is an anti-HER2 nanobody.

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

nanoHER2-SpyTag003

Tags

His6 tag and SpyTag003

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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