30,738 results

1. XX01CAPN2A-c001

   Plasmid

   #175492

   Purpose

   Protein expression in bacterial cells. Entire CDS, M1-L700 (CAPN2); EF hand 1-5, H86-S268 (CAPNS1). CAPN2 can be biotinylated. Codon optimised.

   Depositor

   Opher Gileadi

   Insert

   CAPN2 / CAPNS1 (CAPN2 Human)
   

   Tags

   AviTag and His6-TEV

   Expression

   Bacterial

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSF-G1(6/7FTrp)RS

   Plasmid

   #207621

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of 6-Fluoro-L-Tryptophan (6FTrp) and 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codon

   Depositor

   Thomas Huber

   Article

   Qianzhu et al J Am Chem Soc. 2024 Apr 30. doi: 10.1021

   Insert

   tRNA synthetase
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEx-4T-1-YTHDF2

   Plasmid

   #52301

   Purpose

   Encode YTHDF2 isoform 2 with N-terminal GST tag for E. coli expression

   Depositor

   Chuan He

   Article

   Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

   Insert

   YTHDF2 (YTHDF2 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   April 14, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pMRE-Tn5-164

   Plasmid

   #118544

   Purpose

   miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mOrange2
   

   Use

   Minitn5 delivery vector

   Expression

   Bacterial

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMRE-Tn5-166

   Plasmid

   #118546

   Purpose

   miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mCardinal
   

   Use

   Minitn5 delivery vector

   Expression

   Bacterial

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRsetB-PercevalHR

   Plasmid

   #49081

   Purpose

   Bacterial expression vector of N-terminal His-tagged PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

   Depositor

   Gary Yellen

   Article

   Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

   Insert

   PercevalHR
   

   Tags

   7xHis

   Expression

   Bacterial

   Available Since

   Nov. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX6P1-mClover3-anti-mouse-IgG(kappa)-nanobody

   Plasmid

   #138128

   Purpose

   Expresses GST-tagged mClover3-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

   Insert

   mClover3-anti-mouse-IgG(kappa)-nanobody
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. HC-M

   Plasmid

   #173481

   Purpose

   An rrnB P1-based GFP ATP reporter in E. coli

   Depositor

   Rahul Sarpeshkar

   Article

   Deng et al BMC Biol. 2021 May 17;19(1):101. doi: 10

   Insert

   GFP-mut2
   

   Use

   Synthetic Biology

   Tags

   ssrA degradation tag

   Expression

   Bacterial

   Promoter

   rrnB P1

   Available Since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. KDM4D

   Plasmid

   #73247

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   KDM4D (KDM4D Human)
   

   Tags

   His-6-TEV

   Expression

   Bacterial

   Mutation

   pfam02375:JmjN 18-64, pfam02373:JmjC 182-295

   Promoter

   T7

   Available Since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTK-enhNC1.1_M3-MCS-2A-eGFP-SV40pA

    Plasmid

    #110203

    Purpose

    Recipient vector for Cranial Neural Crest-specific expression of protein of interest

    Depositor

    Tatjana Sauka-Spengler

    Insert

    enhNC1.1_M3-TF_MCS-2A-eGFP-SV40pA
    

    Tags

    eGFP

    Expression

    Bacterial

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYTRW22K_7Ti1

    Plasmid

    #177293

    Purpose

    genomic integration of genes (inserted in I-SceI-site) via rrn-interposon with TcR and LacZ

    Depositor

    Karl-Erich Jaeger

    Article

    Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

    Insert

    lacZ
    

    Use

    Synthetic Biology; Yeast expression, rrn genomic …

    Expression

    Bacterial

    Available Since

    Aug. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMulti_S_ccdB

    Plasmid

    #223853

    Purpose

    GEC-acceptor for 4G-cloning, designed for expression in E. coli (Streptomycin resistance)

    Depositor

    Stephan Gruber

    Article

    Taschner et al bioRxiv 2024.06.17.599261

    Type

    Empty backbone

    Use

    Golden-gate acceptor

    Expression

    Bacterial

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX2T-TEV CHMP1B 1-143

    Plasmid

    #108286

    Purpose

    Expresses residues 1-143 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-278

    Depositor

    Wesley Sundquist

    Article

    Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

    Insert

    CHMP1B (CHMP1B Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    amino acids 1-143

    Available Since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-Trx-mSA2

    Plasmid

    #52320

    Purpose

    Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as a thioredoxin fusion. App Microbiol & Biotech 98, 6285-6295 (2014)

    Depositor

    Sheldon Park

    Article

    Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6

    Insert

    monomeric streptavidin 2
    

    Tags

    FLAG, His, S tag, TEV site, and Thioredoxin

    Expression

    Bacterial

    Mutation

    S25H mutation compared to streptavidin

    Promoter

    T7

    Available Since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRPN265NB-LaG16-MNase

    Plasmid

    #170978

    Purpose

    Expresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & Run

    Depositor

    Marc Timmers

    Article

    Koidl et al Curr Protoc. 2021 Oct;1(10):e266. doi: 1

    Insert

    Glutathione-S-Transferase/LaG16/MNase
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBADmod1-linker2-gamS

    Plasmid

    #45833

    Depositor

    Richard Murray, Vincent Noireaux

    Article

    Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

    Insert

    gamS
    

    Use

    Synthetic Biology

    Tags

    Histag

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSUMO1

    Plasmid

    #52258

    Purpose

    Bacterial expression of human 6His-tagged SUMO1, SUMO-E1 and SUMO-E2

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Inserts

    SUMO-1 (SUMO1 Human)
    UBE2l (UBE2I Human)
    SAE-2 (UBA2 )
    SAE-1 (SAE1 Human)
    

    Tags

    6His-tag

    Expression

    Bacterial

    Mutation

    C-terminal gly-gly

    Promoter

    T7 and rbs

    Available Since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. MP1

    Plasmid

    #69627

    Purpose

    Untargetted Bacterial Mutagenesis

    Depositor

    David Liu

    Article

    Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

    Insert

    dnaQ926 umuD' umuC recA730
    

    Expression

    Bacterial

    Promoter

    E. coli PBAD

    Available Since

    Sept. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET StrepII-StrepII TEV LIC cloning vector (2RRT)

    Plasmid

    #55215

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    2xStrep II and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pE-SUMO CRYGD

    Plasmid

    #80755

    Purpose

    Expression of human gamma D-crystallin in E. coli

    Depositor

    Larry David

    Insert

    CRYGD (CRYGD Human)
    

    Tags

    6xHIS and Smt3 SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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