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32,069 results

Showing: 10681 - 10690 of 32069 results

  1. pTXB1-H2B(1-114)_wt

    Plasmid
    #118252
    Purpose
    Bacterial expression plasmid encoding X. laevis H2B residues 1-114 as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNa
    Depositor
    Cynthia Wolberger
    Article
    Morgan et al Science. 2016 Feb 12;351(6274):725-8. do
    Insert
    xlH2B(1-114)
    Tags
    Mxe-GyrA intein fused to chitin binding domain
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    118252-plasmid-map-sequence-id-226720

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  2. pJSC281 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant

    Plasmid
    #101230
    Purpose
    Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/E60C/D273C/N692A/M694A/Q695A/H698A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, E60C, D273C, N692A, M694A, Q695A and…
    Promoter
    T7
    Available Since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    101230-plasmid-map-sequence-id-198024

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  4. pNSSCP

    Plasmid
    #183188
    Purpose
    PACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; DivIVA-sfGFP expressed under T
    Depositor
    Hsiao-Chun Huang
    Article
    Lin et al Nat Commun. 2021 Feb 9;12(1):888. doi: 1
    Inserts
    DivIVA and superfolder GFP fusion protein
    SpmX450 and T7 181+ fusion protein
    T7 RNAP 1-179 variant and SpmX450 fusion protein
    mRFP1 and popZ fusion protein
    Expression
    Bacterial
    Available Since
    July 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    183188-plasmid-map-sequence-id-366663

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  6. pETM-11-HsPABPC1_J

    Plasmid
    #146642
    Purpose
    Bacterial Expression of HsPABPC1
    Depositor
    Elisa Izaurralde
    Insert
    HsPABPC1 (PABPC1 Human)
    Expression
    Bacterial
    Mutation
    two silent mutations compared to the sequence giv…
    Available Since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146642-plasmid-map-sequence-id-396951

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  10. pSiM1

    Plasmid
    #199380
    Purpose
    Encodes dual cistronic membrane localized GCaMP6s and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    GCaMP6s::ras-2CAAX::SL2::mScarlet-I::ras-2CAAX
    Expression
    Bacterial
    Mutation
    no mutations, except added subcellular localizati…
    Available Since
    April 24, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    199380-plasmid-map-sequence-id-391037

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Showing: 10681 - 10690 of 32069 results