31,663 results
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Plasmid#72678PurposeExpresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Kan resistance marker.DepositorInsertmutL E32K
TagsnoneExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…Available SinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
CpxR-GFP
Plasmid#220150PurposeTo track the natural CpxR promoter's transcriptional dynamicsDepositorInsertCpxR promoter only
ExpressionBacterialAvailable SinceAug. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBSK
Plasmid#67504PurposeExpress the tRNALys scaffoldDepositorInsertmodified tRNALys human
ExpressionBacterialPromoterlppAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSLIK CA RhoA
Plasmid#112894Purposepromoter-inducible expression of constitutively-active RhoADepositorAvailable SinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTP1172
Plasmid#104161PurposeBacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (3x Cysteines)DepositorInsertAnti-mouse kappa chain specific nanobody TP1170 (3xCysteines)
Tags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialAvailable SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lwa_Cas13a_WT
Plasmid#91865PurposeBacterial expression for Cas13aDepositorInsertLwaCas13a
UseCRISPRTagsHis6-MBP-TEV siteExpressionBacterialPromoterT7Available SinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-mNeongreen-Uricase
Plasmid#182417PurposeBacterial expression of Vesicle Nucleating peptide-mNeongreen-uricase fusionDepositorInsertVNp-mNeongreen-uricase
TagsVesicle Nucleating peptide (VNp) & mNeongreenExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-PsppA-mCherry
Plasmid#225428PurposePlasmid encodes SgRNA, SpCas9 and homologous arms for homologous recombination of mCherryDepositorInsertmCherry, Cas9, SgRNA
UseCRISPRExpressionBacterialPromoterPsppA upstream of mCherry and Cas9, P3 upstream o…Available SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
Ydf NanoLuc
Plasmid#225656PurposeReporter plasmid expressing NanoLuciferase under the control of the promoter PydfJ115, cognate to YdfI. Responds to NarL/NarX chimeras from this study.DepositorInsertNanoLuciferase
UseCell free expressionTagsMycExpressionBacterialPromoterPydfJ115Available SinceDec. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL NarL YdfI Flag
Plasmid#225650PurposeExpresses NarL with a mutated DNA binding domain (YdfI). For use with NarX chimeras and reporter plasmids from this study.DepositorInsertNarL-YdfI (narL Synthetic)
UseCell free expressionTagsFLAGExpressionBacterialMutationNarL with native DNA-binding domain replaced with…PromoterT7Available SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL NarX Myc
Plasmid#225651PurposeExpresses WT NarX capable of sensing nitrate. For use with NarL-YdfI and reporter plasmids from this study.DepositorAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRPN265NB-enh-MNase
Plasmid#166035PurposeExpresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & RunDepositorInsertGlutathione-S-Transferase/enhancer/MNase
TagsGSTExpressionBacterialPromoterlac promoter / tac promoterAvailable SinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOlyA(WT)
Plasmid#132391PurposeOstreolysin A for sensing sphingomyelin/cholesterol complexesDepositorInsertOstreolysin A wild-type
TagsHis6 tagExpressionBacterialMutationMutations: C62S, C94S, S151CAvailable SinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-PA-Rac1
Plasmid#22027PurposeExpression of photoactivatable Rac1DepositorInsertPA-Rac1 (RAC1 Human, Avena sativa (oat))
Tags6xHis and mCherryExpressionBacterial, Insect, and Mamm…MutationLOV(Leu404-Leu546), Rac1 starts at Ile4 and conta…PromoterCMV, p10Available SinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCMV/SB11
Plasmid#26552DepositorInsertSleeping Beauty
ExpressionBacterialMutationMethionine 243 to Glutamine Threonine 136 to Arg…Available SinceOct. 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET21a-HisRS-His
Plasmid#124111PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable SinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
AP1
Plasmid#179678PurposeAccessory plasmidsDepositorInsert[SD8] gIII
ExpressionBacterialAvailable SinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
p189
Plasmid#65207PurposeMutant EGFP expression plasmid for the creation of a heteroduplex DNA repair assay plasmid with pGEM5Z(+)-EGFP (p111). *Please see notes in comments section.DepositorInsertEGFP
ExpressionBacterial and MammalianMutationPremature stop codon added to pGEM5Z(+)-EGFP (TGG…PromoterCMVAvailable SinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEM5Z(+)-EGFP
Plasmid#65206PurposeEGFP expression plasmid (also called p111) for the creation of a heteroduplex DNA repair assay plasmid with p189. *Please see notes in comments section.DepositorInsertEGFP
ExpressionBacterial and MammalianMutationRestriction enzyme recognition sites added either…PromoterCMVAvailable SinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2(+)-zGrad
Plasmid#119716PurposePlasmid to generate zGrad mRNA to inactivate proteins in zebrafishDepositorInsertzGrad
ExpressionBacterialAvailable SinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only