30,738 results

1. pTriEx-NTOM20-mVenus-Zdk1

   Plasmid

   #81010

   Purpose

   Expression of Zdk1 anchored to mitochondria

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Insert

   Zdk1
   

   Tags

   6x His tag, FLAG, NTOM20, and mVenus

   Expression

   Bacterial, Insect, and Mamm…

   Available Since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEXP5-NT/6xHis mCherry F30 2xdBroccoli

   Plasmid

   #169233

   Purpose

   Constitutive T7 RNAP-mediated expression of 6xHis-mCherry with 2xd Broccoli RNA aptamer.

   Depositor

   Dora Tang

   Article

   Gonzales et al ACS Synth Biol. 2022 Jan 21;11(1):205-21

   Insert

   6xHis mCherry F30-2xdBroccoli
   

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Dec. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. C14orf169

   Plasmid

   #39114

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   C14orf169 (C14orf169 Human)
   

   Tags

   Tev His Flag

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pTD103luxI_sfGFP

   Plasmid

   #48885

   Purpose

   Positive feedback plasmid from oscillator

   Depositor

   Jeff Hasty

   Article

   Prindle et al Nature. 2011 Dec 18;481(7379):39-44. doi

   Insert

   LuxI, sfGFP, and LuxR
   

   Use

   Synthetic Biology

   Tags

   ssrA tag (AANDENYALAA)

   Expression

   Bacterial

   Promoter

   pLux

   Available Since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBAD_sfGFP3TCG4TAG

   Plasmid

   #174518

   Purpose

   Recoded sfGFP reporter with the codons TCG and TAG inserted at positions 3 and 4, respectively.

   Depositor

   Jason W Chin

   Article

   Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

   Insert

   sfGFP
   

   Tags

   6 x His

   Expression

   Bacterial

   Mutation

   Recoded. TCG and TAG codons were introduced in po…

   Promoter

   araBAD

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pORTMAGE-Pa1

   Plasmid

   #138475

   Purpose

   Enables high-efficiency recombineering in P. aeruginosa

   Depositor

   George Church

   Article

   Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

   Inserts

   PapRecT
   mutL E36K
   

   Expression

   Bacterial

   Mutation

   E36K

   Available Since

   June 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAD-KPS12-Pman

   Plasmid

   #133832

   Purpose

   Fluorescent protein mKate(KPS12) variant for optimized expression in Bacillus mycoides; under control of mannose inducible promoter.

   Depositor

   Oscar Kuipers

   Article

   Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

   Insert

   mKate(KPS12)
   

   Use

   E.coli/bacillus spp. shuttle vector

   Expression

   Bacterial

   Promoter

   Pman

   Available Since

   Nov. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNCS mTangerine

   Plasmid

   #91763

   Purpose

   Bacterial expression of a fluorescent protein, monomeric Tangerine (mTangerine)

   Depositor

   Roger Tsien, Erik Rodriguez

   Article

   Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

   Insert

   monomeric Tangerine
   

   Tags

   no tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pttq18

   Plasmid

   #69122

   Purpose

   regulated expression of genes in Escherichia coli

   Depositor

   Michael Stark

   Article

   Stark et al Gene. 1987;51(2-3):255-67.

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Oct. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUS116

    Plasmid

    #84578

    Purpose

    Shuttle and expression vector. Replicates in E.coli and Mycobacterium. Gives acetamide-inducible gene expression in Mycobacterium.

    Depositor

    Nicholas Coleman

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Jan. 17, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pTriEx-mCherry-PA-Rac1

    Plasmid

    #22027

    Purpose

    Expression of photoactivatable Rac1

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1 (RAC1 Avena sativa (oat), Human)
    

    Tags

    6xHis and mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    LOV(Leu404-Leu546), Rac1 starts at Ile4 and conta…

    Promoter

    CMV, p10

    Available Since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET30-2-GPD1L

    Plasmid

    #83912

    Purpose

    stable overexpression

    Depositor

    David Sabatini

    Article

    Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

    Insert

    glycerol-3-phosphate dehydrogenase 1-like (GPD1L Human)
    

    Tags

    6x His tags

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC-GFP-AT

    Plasmid

    #133306

    Purpose

    "Burdensome" GFP expressing plasmid carrying axe/txe toxin-antitoxin for plasmid stabilisation

    Depositor

    Chris Barnes

    Article

    Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1

    Insert

    axe/txe toxin-antitoxin cassette
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. +36GFP-LPETG

    Plasmid

    #71754

    Purpose

    +36GFP-LPETG for expression in E.Coli

    Depositor

    David Liu

    Article

    Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

    Insert

    (GGS)9_(pos36GFP)_6XHis_LPETG
    

    Tags

    6XHis and Sortase (LPETG)

    Expression

    Bacterial

    Available Since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pExp-GB1

    Plasmid

    #112565

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GB1 fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Tags

    8xHis-GB1 and Strep tag

    Expression

    Bacterial

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. PET30B-PTEN

    Plasmid

    #20741

    Depositor

    Alonzo Ross

    Article

    Redfern et al Biochemistry. 2008 Feb 19. 47(7):2162-71

    Insert

    PTEN (PTEN Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    April 2, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pHEN-CoV2-nanobody-G6

    Plasmid

    #194589

    Purpose

    Bacterial expression of G6 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

    Depositor

    Gerald McInerney

    Article

    Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

    Insert

    G6 (S )
    

    Expression

    Bacterial

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-21a(+)-IPP1-His

    Plasmid

    #118978

    Purpose

    Bacterial expression plasmid for Inorganic pyrophosphatase

    Depositor

    Sebastian Maerkl

    Article

    Lavickova et al ACS Synth Biol. 2019 Jan 11. doi: 10.102

    Insert

    inorganic diphosphatase IPP1 (IPP1 Budding Yeast)
    

    Tags

    6x-His

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. dCas9-CRY2Clust-FUSN

    Plasmid

    #200967

    Purpose

    induction of phase-separated condensate mediated by FUSN-IDR

    Depositor

    Jian Xu

    Article

    Kim et al Sci Adv. 2023 Mar 31;9(13):eadg1123. doi

    Insert

    FUSN
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAD-sfGFP(Sp)-Pman

    Plasmid

    #133833

    Purpose

    Fluorescent protein sfGFP(Sp); under control of mannose inducible promoter.

    Depositor

    Oscar Kuipers

    Article

    Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

    Insert

    sfGFP(Sp)
    

    Use

    E.coli/bacillus spp. shuttle vector

    Expression

    Bacterial

    Promoter

    Pman

    Available Since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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