33,902 results
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Plasmid#120229PurposeTAR cloning in yeast and low-copy maintenance of large constructs in E. coliDepositorTypeEmpty backboneExpressionBacterialAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pJMP1356
Plasmid#119276Purposefor cloning new sgRNAsDepositorInsertdcas9
ExpressionBacterialMutationD10A and H840AAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDSG372
Plasmid#115602Purposesurface-displayed Nb1; medium copyDepositorInsertregulated adhesin construct
ExpressionBacterialAvailable sinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTU2-AmyE-Pveg-eGFP
Plasmid#112792PurposeExpresses GFP under the control of Pveg promoter and it is integrated into AmyE locus. Used to test correct transformationDepositorPublicationInsertPveg-eGFP
ExpressionBacterialAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSANG14-3F
Plasmid#39265DepositorPublicationTypeEmpty backboneTags6xHis, Alkaline phosphatase, pelB leader, and tri…ExpressionBacterialPromoterT7Available sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBAD-GINKO1
Plasmid#113111PurposeBacterial expression of genetically encoded green fluorescent potassium ion indicator GINKO1DepositorInsertGINKO1
Tags6-His Tag, T7 tag (gene 10 leader), and Xpress™ t…ExpressionBacterialPromoteraraBADAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-APEX2
Plasmid#109420PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to ascorbate peroxidase 2. VHH-APEX2 also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, APEX2, HA, BAP and His6 epitope
TagsBAP, HA, His6, and T7ExpressionBacterialPromoterT7Available sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lbpro (29-195)
Plasmid#110759PurposeBacterial expression of LbproDepositorInsertLb pro
ExpressionBacterialPromoterT7Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBx-Spas-sgRNA-Kan
Plasmid#105233PurposePlasmid containing S. pasteurianus sgRNA with no 20bp target (Empty control) Kanamycin marker for P. putida and P. fluorescensDepositorInsertS. pasteurianus sgRNA
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SUMO-DogTag
Plasmid#105629PurposePlasmid for recombinant expression in bacteria of SUMO domain fused to DogTag, permitting covalent linkage to SnoopTagJrDepositorInsertSUMO-DogTag
ExpressionBacterialPromoterT7Available sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
phage UbiC scAB-GFP
Plasmid#104998PurposeLentiviral vector expressing sequence-optimized single chain Antibody-GFP fusion protein for SunTag labelingDepositorInsertsingle chain Antibody against GCN4
UseLentiviralTagsGB1, HA tag, and sfGFPExpressionBacterial and MammalianPromoterUbiCAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pQE31-rsEGFP2
Plasmid#102879PurposeExpresses rsEGFP2 in bacterial cellsDepositorInsertrsEGFP2
Tags6xHisTagExpressionBacterialAvailable sinceJan. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQE80L-IL2 C125S
Plasmid#104348PurposeExpresses human IL-2 (C125S)DepositorInsertHuman IL-2 (C125S) (IL2 Human)
TagsMRGSHHHHHGS-tagExpressionBacterialMutationC125S mutation (TGT -> TCC)PromoterT5Available sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETDuet RagA-RagC
Plasmid#99641PurposeBacterial expression of wildtype Rag GTPase dimerDepositorAvailable sinceDec. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mKate2
Plasmid#104009PurposePlasmid encoding mKate2DepositorInsertmKate2
UseLow copyExpressionBacterialMutationMutations relative to eqFP578 (R32G, K42R, V45A, …PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJSC111 - Bacterial expression plasmid for SpCas9-HF1 variant
Plasmid#101209PurposeBacterial expression plasmid for SpCas9-HF1 variantDepositorInsertSpCas9 variant N497A/R661A/Q695A/Q926A
Tags10x His, MBP, and TEV siteExpressionBacterialMutationN497A, R661A, Q695A and Q926APromoterT7Available sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Capping Protein
Plasmid#89950PurposeF-actin-capping proteins bind in a Ca2+-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends.DepositorInsertsTags6*HisExpressionBacterialMutationV222IAvailable sinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pNCS mHoneydew
Plasmid#91760PurposeBacterial expression of a fluorescent protein, monomeric Honeydew (mHoneydew)DepositorInsertmonomeric Honeydew
Tagsno tagsExpressionBacterialPromoterT7Available sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEM-Cas9HF1
Plasmid#89961PurposeModified from pCas9-CR4 (Addgene: 62655) to use the high-fidelity version of Cas9, SpCas9-HF1 (N497A/R661A/Q695A/Q926A) from Kleinstiver et al 2016.DepositorInsertCas9HF1
ExpressionBacterialMutationN497A/R661A/Q695A/Q926APromoterpTetAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCFJ1955
Plasmid#84830PurposeMinimos transposon with Peft-3:tdTomato:tbb-2 3'UTR and cbr-unc-119 selection. tdTomato was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic intronsDepositorInsertC. elegans codon optimized tdTomato
ExpressionBacterial and WormPromoterPeft-3Available sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by