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pKO403_lacZ_Cm

Plasmid

#174725

Purpose

Bifidobacterium-E.coli shuttle vector for seamless gene cloning

Depositor

Tohru Suzuki

Article

Altaib et al Microbiol Resour Announc. 2022 Jan 20;11

Type

Empty backbone

Expression

Bacterial

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pJKR-H-tetR

Plasmid

#62561

Purpose

Tetracycline biosensor. High copy plasmid that expresses GFP proportional to tetracycline concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pTriExRhoA2G

Plasmid

#40176

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

RhoA Biosensor

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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pHCL151

Plasmid

#134455

Purpose

TetR, lacI-Plac-ssdsbA-mscarN, Integrative plasmid for the expression of fully-screted prey. N-terminal mScarlet-I fusion.

Depositor

Thomas Bernhardt

Article

Lim et al Mol Microbiol. 2019 Sep 24. doi: 10.1111

Insert

linker-mScarlet-I for C-terminal fusion

Expression

Bacterial

Promoter

plac

Available Since

Jan. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pBbA6c-RFP

Plasmid

#35290

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

LlacO-1

Available Since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pFNR5

Plasmid

#149483

Purpose

Broad-Host range expression vector, RSF1010 origin, NahR(AM) repressor, PSalTTC promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

NahR(AM)-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PSalTTC

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pET32a-hTGFa

Plasmid

#199236

Purpose

Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

Depositor

Nilson Zanchin

Article

Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

Insert

Transforming growth factor alpha, partial (TGFA Human)

Tags

TrxA-6xHis-S-tag

Expression

Bacterial

Promoter

T7

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pET21a.Tat(48-57)-EGFP

Plasmid

#165040

Purpose

Expression of Tat(48-57) conjugated EGFP for protein purification (6XHis tag)

Depositor

Dokyun Na

Article

Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

Insert

EGFP

Tags

6XHis and Tat(48-57)

Expression

Bacterial

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_YWHAG_14-3-3

Plasmid

#109905

Purpose

Protein expression and purification of YWHAG_14-3-3

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

YWHAG_14-3-3 (YWHAG Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-Gm-Pc-DsRed-Express2

Plasmid

#111260

Purpose

mini-Tn7 vector for insertion of DsRed-Express2 at bacterial attTn7 site

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

DsRed-Express2

Expression

Bacterial

Promoter

Pc

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pJYScr

Plasmid

#132719

Purpose

Plasmid for an easy-to-construct crRNA delivery vector using hybridized oligonucleotides. Constitutive transcription of crRNA, Spectinomycin resistance

Depositor

Jan Marienhagen

Article

Krumbach et al ACS Synth Biol. 2019 Dec 11. doi: 10.102

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pMVV099

Plasmid

#215305

Purpose

Expresses sfGFP under induction with the Cuminic Acid Marionette Cassette (CymRAM + PCymRC-sfGFP) with a BBR1 backbone.

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

sfGFP

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Promoter

PCymRC

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pHLUK

Plasmid

#64181

Purpose

harbours marker genes HIS3, LEU2, URA3 and LYS2

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pAWP78

Plasmid

#61263

Purpose

Small IncP-based broad host range replicating vector.

Depositor

Mary Lidstrom

Article

Puri et al Appl Environ Microbiol. 2014 Dec 29. pii

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 26, 2015

Availability

Academic Institutions and Nonprofits only

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p006-GFP-pBAD

Plasmid

#108315

Purpose

GFP from jellyfish Aquorea sp. under pBAD promoter with AraC repressor; kanamycin resistant.

Depositor

Jaroslaw Bryk

Article

Siddall et al bioRxiv 2021.06.20.449138

Insert

GFP under control of arabinose-inducible promoter pBAD

Expression

Bacterial

Promoter

pBAD

Available Since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST14-SpyCatcher002

Plasmid

#102827

Purpose

Bacterial expression of SpyCatcher002 for covalent coupling to SpyTag002-linked proteins

Depositor

Mark Howarth

Article

Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

Insert

SpyCatcher002

Tags

GSSGS, His6, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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p6XHis_NLS-SaCas9

Plasmid

#101086

Purpose

Express SaCas9 in bacteria with a 6xHis tag for purification

Depositor

Rick Tarleton

Article

Soares Medeiros et al MBio. 2017 Nov 7;8(6). pii: e01788-17. d

Insert

CRISPR-associated protein Cas9/Csn1

Use

CRISPR

Tags

TrxA-6xHis-STag

Expression

Bacterial

Promoter

T7lac

Available Since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pRsetB-PercevalHR

Plasmid

#49081

Purpose

Bacterial expression vector of N-terminal His-tagged PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

Depositor

Gary Yellen

Article

Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

Insert

PercevalHR

Tags

7xHis

Expression

Bacterial

Available Since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pDule-tfmF A65V S158A

Plasmid

#85484

Purpose

Machinery plasmid expressing permissive Mj tfmF synthetase and cognate amber suppressing tRNA

Depositor

Ryan Mehl

Article

Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.

Insert

tri-fluoromethyl-phenylalanine Mj synthetase A65V S158A

Tags

None

Expression

Bacterial

Mutation

Y32V F108W Q109M I159M with 65Val and 158Ala

Promoter

lpp (constitutive)

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pMLS7

Plasmid

#32056

Purpose

Vector optimized for constitutive gene expression in Burkholderia genus and related bacteria

Depositor

Miguel Valvano

Article

Lefebre et al Appl Environ Microbiol. 2002 Dec;68(12):

Type

Empty backbone

Expression

Bacterial

Promoter

Ps7 promoter, originated from Paraburkholderia xe…

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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