30,576 results

1. pATetO 6XHis-Taq DNA Pol

   Plasmid

   #178782

   Purpose

   Inducible expression of Taq DNA polymerase in E. coli

   Depositor

   Andrew Ellington

   Article

   Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

   Insert

   Taq DNA Polymerase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. LgBiT-ST

   Plasmid

   #207633

   Purpose

   A plasmid encoding LgBiT fused to SpyTag

   Depositor

   Cole DeForest

   Article

   Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

   Insert

   LgBiT-SpyTag
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGT-S1

   Plasmid

   #122582

   Purpose

   MAGIC donor plasmid (RSF1010 oriR with beta-lactamase/sfGFP payload)

   Depositor

   Harris Wang

   Article

   Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

   Insert

   beta-lactamase/sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pQE30-His-PheRS

   Plasmid

   #124116

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Phenylalanyl-tRNA ligase (pheS, pheT Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. plYJP022-int7

   Plasmid

   #202287

   Purpose

   Plasmid containing integrase for LPattB7 site

   Depositor

   Christopher Voigt

   Article

   Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

   Insert

   int7
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. SPLICSFAST-NU-MT-P2A

   Plasmid

   #214898

   Purpose

   Expresses equimolar Nucleus- and Mitochondria- targeted split-FAST fragments for Short-range NU-MT Contact sites

   Depositor

   Tito Calì

   Insert

   SPLICSFAST-NU-MT-P2A
   

   Use

   Tags

   none

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pC018 - LshCas13a from Leptotrichia shahii

   Plasmid

   #91908

   Purpose

   Expresses LshCas13a for bacterial expression and contains backbone for spacer cloning

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   LshCas13a and guide expression cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSL-MS2-6X

   Plasmid

   #27118

   Depositor

   Robert Singer

   Article

   Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

   Insert

   MS2 binding sites (6)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSB235

   Plasmid

   #164130

   Purpose

   PhlF-RFP expression plasmid

   Depositor

   Christopher Voigt

   Article

   Shao et al Nat Commun. 2021 Mar 5;12(1):1475. doi:

   Insert

   PhlF-RFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptet

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pACYC-Taq

    Plasmid

    #102793

    Purpose

    Expresses Taq DNA polymerase under WT T7 promoter

    Depositor

    Andrew Ellington

    Article

    Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

    Insert

    Taq DNA polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAKlux2

    Plasmid

    #14080

    Depositor

    Attila Karsi

    Article

    Karsi et al Plasmid. 2007 May;57(3):286-95. doi: 10.

    Insert

    Bacterial luciferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac, T3

    Available Since

    March 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBB550

    Plasmid

    #27396

    Depositor

    Bernd Bukau

    Article

    de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

    Insert

    dnaK, dnaJ, GroESL
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHAGE2-TetOminiCMV-OSK

    Plasmid

    #136552

    Purpose

    Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Sox2, Klf4

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Oct4-P2A-Sox2-T2A-Klf4 (Klf4, Sox2, Pou5f1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tetO-miniCMV (dox-inducible)

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAP05

    Plasmid

    #102920

    Purpose

    Direct cloning and broad-host-range heterologous expression vector

    Depositor

    Bradley Moore

    Article

    Zhang et al MBio. 2017 Sep 5;8(5). pii: e01291-17. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-His-Cre

    Plasmid

    #123128

    Purpose

    Bacterial expression plasmid for wild-type Cre with an N-terminal His-tag.

    Depositor

    David Liu

    Article

    Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

    Insert

    Cre recombinase
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET21a-MetRS-His

    Plasmid

    #124115

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Methionine--tRNA ligase (metG Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pVP144

    Plasmid

    #222081

    Purpose

    Single component CRISPR-mediated base-editor for Agrobacterium genetic engineering visually marked with sfGFP for guide insertion and eforRED for plasmid eviction.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al Biodes Res. 2025 March, 7:100001. doi: 1

    Insert

    dCas9-PmCDA1, sfGFP, sacB and eforRED
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDR111_Bgal no NLS

    Plasmid

    #188397

    Purpose

    Expresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.

    Depositor

    Christopher Contag

    Article

    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

    Insert

    lacZ
    

    Use

    Tags

    PhoD secretion peptide

    Expression

    Bacterial

    Mutation

    Promoter

    Phyper spank

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET16b-His-ArgRS

    Plasmid

    #124104

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Argin+I4 ligase (argS Escherichia coli)
    

    Use

    Tags

    10x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. S48I48 ELP

    Plasmid

    #68394

    Purpose

    Elastin-like polypeptide (VPGxG) with 48 repeats of serine guest residue followed by 48 repeats of isoleucine guest residue

    Depositor

    Andrew MacKay

    Article

    Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

    Insert

    S48I48 ELP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge