33,434 results
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Plasmid#105234PurposeTn7 integrating plasmid with S. pasteurianus dCas9 expressed from the Ptac promoter for expression in P. putida and P. fluorescensDepositorInsertS. pasteurianus dCas9
UseCRISPR and Synthetic BiologyTagsHAExpressionBacterialMutationD10A, H599AAvailable sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNS20-SpCas9-SNAP
Plasmid#113717PurposeBacterial vector for expression of Snap-tagged Streptococcus pyogenes Cas9DepositorInsertCas9
UseCRISPRTagsHA-2xNLS-SNAP-NLS and His6-MBP-TEVExpressionBacterialAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LIC1B_Caspase-LOVN7C-7
Plasmid#104629Purposebacterial expression of light-activated caspase-3 with N7C-7 linker variationDepositorAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAJM.1642
Plasmid#108535PurposeCinRAM + PCin-YFP Reporter. OHC14 sensor for use in non-Marionette strain.DepositorInsertCinRAM + PCin-YFP
ExpressionBacterialMutationCinR: S72C V80I G114D G115D R137H K220R A224T *24…Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSLIK CA RhoA
Plasmid#112894Purposepromoter-inducible expression of constitutively-active RhoADepositorAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRK2-AraE
Plasmid#110141PurposeConstitutive promoter expressing araE on RK2 originDepositorInsertaraE
ExpressionBacterialAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCK306
Plasmid#110544PurposerhaBAD, a rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteria.DepositorInsertsPrhaBAD
yfp
rhaS
ExpressionBacterialPromoterN/A, PrhaBAD, and kanR promoter (upstream of kanR…Available sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJM101(pUC18T-miniTn7T-gm-lacIq-Ptac)
Plasmid#110558PurposeminiTn7 delivery plasmid with lacIq-Ptac inducible promoterDepositorInsertlacIq-Ptac
ExpressionBacterialPromoterlacIq-PtacAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pIB184-Km
Plasmid#90195PurposeE. coli - Streptococci shuttle plasmid for gene expression in streptococci with P23 promoterDepositorTypeEmpty backboneTagsNoneExpressionBacterialAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pIB166
Plasmid#90189PurposeE. coli - Streptococci shuttle plasmid for gene expression in streptococci with P23 promoterDepositorTypeEmpty backboneTagsNoneExpressionBacterialAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSMART-SOS-GFPuv
Plasmid#102283PurposeFluorescent sensor of E. coli SOS responseDepositorInsertcda-GFPuv
ExpressionBacterialPromotercda SOS promoterAvailable sinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a_T5-ARG1
Plasmid#106476PurposeExpresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli Nissle 1917DepositorInsertARG1
UseSynthetic BiologyExpressionBacterialPromoterT5Available sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a-SnoopTagJr-MBP
Plasmid#105628PurposePlasmid for recombinant expression in bacteria of MBP fused to SnoopTagJr, permitting covalent linkage to DogTag by SnoopLigaseDepositorInsertSnoopTagJr-MBP
ExpressionBacterialPromoterT7Available sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBad-HPII
Plasmid#105839Purposeexpresses E. coli HPII catalase with N-terminal 6x his tagDepositorPublicationInserthydroperoxidase II catalase
Tags6x his tagExpressionBacterialPromoterpBad/arabinoseAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAD-LyseR (in lacZ deficient strain)
Plasmid#99245PurposeFor autolysate production in lacZ deficient (lac operon deletion) E. coli BL21-Gold-dLac (DE3) strain: constitutively expresses phage lambda endolysin (gene R)DepositorAvailable sinceOct. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
XXBLM (XXBLMA-c075)
Plasmid#98223PurposeVector for periplasmic expression of Lama antibodies with PelA leader and C-terminal His6 tagDepositorAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET21-Flavidin
Plasmid#89881PurposeFluorophore-friendly streptavidin that can improve fluorescent brightness and has unimpaired biotin binding after dye labelingDepositorInsertStreptavidin-K80R-K121R-K132R-K134R-Glutamate_Tag
Tags6 glutamate tag (C terminal on insert)ExpressionBacterialMutationK80R, K121R, K132R, K134RAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZA31-sulA-GFP
Plasmid#78493PurposeExpression of GFP under the sulA promoter.DepositorInsertGFPmut2
ExpressionBacterialAvailable sinceApril 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDule-para-aminoPhe
Plasmid#85502PurposePlasmid for incorporating the non-canonical amino acid para-aminoPhe with the Mj pAF synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsertp-aminoPhe tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
TagsNoneExpressionBacterialMutationY32T, E107T, D158P, I159L, L162APromoterlpp (constitutive)Available sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDB180
Plasmid#80677Purposefor secretion of N-terminal OsmY, deca-histidine, SUMO tagged proteinDepositorTypeEmpty backboneTagsOsmY-10His-Smt3ExpressionBacterialPromoterT7Available sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by