31,059 results
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Plasmid#158753PurposeBacterial expression of 6xHis-A1aY1 fused with carboxy terminus GFP for recombinant purificationDepositorInsert6xHistidine_A1aY1_GFP
Tags6x-Histidine tag, GFP, T7 leader sequence, and Th…ExpressionBacterialPromoterT7Available SinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTP1005
Plasmid#104160PurposeBacterial expression plasmid of anti-mouse IgG2a Fc nanobody TP1129 (3x Cysteines)DepositorInsertAnti-mouse IgG2a Fc specific nanobody TP1129 (3xCysteines)
Tags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialAvailable SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNB22
Plasmid#103059Purposemedium copy plasmid (pBR322 backbone) expressing the unstable GFP variant GFP-OVA under control of the nitric oxide-inducible promoter hmpApDepositorInsertGFP-OVA
TagsnoneExpressionBacterialPromoterhmpApAvailable SinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
GB1-A11(2-88)-Strep
Plasmid#196214PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-88)-Strep.DepositorAvailable SinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-6XHis-SUMO-SIRT2
Plasmid#102622PurposeExpresses a truncation (aa38-356) of human SIRT2 isoform 1 in bacteria for purificationDepositorInsertSIRT2 (SIRT2 Human)
Tags6XHis-SUMOExpressionBacterialMutationDeleted amino acids 1-37 and 357-389Available SinceFeb. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAUX_OL
Plasmid#166246Purposeuse as an auxiliary temperature-sensitive plasmid to successfully transform pdCas9_CL plasmidDepositorInsert[P112-sgRNA-term]-[J23116_B34-dCas9-B15]
ExpressionBacterialPromoterEc-TTL-P112 and BBa_J23116Available SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pDae069 (eMutaT7[PmCDA1] with optimized RBS)
Plasmid#187621PurposeExpresses eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coliDepositorInsertPmCDA1-T7RNAP
ExpressionBacterialAvailable SinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOPINF
Plasmid#26042DepositorInsertHis3C-lacZ
TagsMAHHHHHHSSGLEVLExpressionBacterial, Insect, and Mamm…Available SinceMay 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUNIV backbone
Plasmid#24705DepositorTypeEmpty backboneUseXenopus oocyte expressionExpressionBacterial, Mammalian, and Y…Available SinceDec. 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBBR4pemIK
Plasmid#126928PurposeBroad host range vector with toxin-antitoxin stability system for maintenance in the absence of antibiotic selectionDepositorTypeEmpty backboneExpressionBacterialAvailable SinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-Cys-SpyCatcher002
Plasmid#102829PurposeBacterial expression of Cysteine-modified SpyCatcher002 allowing reaction with thiol coupling reagentsDepositorInsertCys-SpyCatcher002
TagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationCysteine residue in N-terminal regionPromoterT7Available SinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGT-S1
Plasmid#122582PurposeMAGIC donor plasmid (RSF1010 oriR with beta-lactamase/sfGFP payload)DepositorInsertbeta-lactamase/sfGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15A-PLtetO1-tetR-T500
Plasmid#45391DepositorInserttet repressor
ExpressionBacterialPromoterPL-tetO1Available SinceJune 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDAI-SceI-pheS
Plasmid#111611PurposeYeast homing endonuclease I - SceI expression vector. Used for resolution of merodiploids during allelic exchange in Burkholderia sp.DepositorInsertpheS
ExpressionBacterialAvailable SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGJK_His-SUMO-LbuCas13a
Plasmid#172488PurposeBacterial expression of codon-optimized His-SUMO-LbuCas13a.DepositorInsertbdSUMO-LbuCas13a (codon-optimized)
TagsHis6 and bdSUMOExpressionBacterialPromoterT7 promoter and lac operatorAvailable SinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUltra-5HTP
Plasmid#178042PurposeExpress modified ScTrpRS/tRNA for genetic incorporation of 5HTP to TAG codonDepositorInsertsModified ScTrpRS for 5HTP incoporation
suppressor tRNA for 5HTP incorporation
ExpressionBacterialMutationT107C, P254T, C255AAvailable SinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEC3109 (pSpy1C-Plac(1)_ffluc)
Plasmid#218514Purposereplicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressorDepositorInsertffluc
UseLuciferaseExpressionBacterialMutationdeletion of mrfp cassette, mutagenesis of the RBS…PromoterPlac(1)Available SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
Split 'n coexpress CUL3-RBX1
Plasmid#52293Purposebacterial co-expression of N-terminal Cul3, C-terminal Cul3, and Rbx1DepositorTagsGST, IRES, and TEVExpressionBacterialMutationcontains aa 1-309, contains aa 310-768, and conta…PromoterIRES and tacAvailable SinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBad-PhoCl2c
Plasmid#164035PurposeImproved PhoCl variant with enhanced dissociation efficiencyDepositorInsertPhoCl2c
TagsHis tag, T7 tag, Xpress TagExpressionBacterialPromoteraraBad promotorAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only