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pCAT206

Plasmid

#134883

Purpose

Bacterial expression

Depositor

Thomas Howard

Article

Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-143

Plasmid

#118559

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sYFP2

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pOGG202

Plasmid

#115503

Purpose

Clone of a level 1 unit which encodes IPTG-inducible sfGFP

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

pL1M-F1-plac pOGG031, sfGFP pOGG037, T-pharma pOGG003

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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IFI16-FL-(1-729)

Plasmid

#35051

Depositor

Cheryl Arrowsmith

Article

Liao et al Structure. 2011 Mar 9;19(3):418-29.

Insert

IFI16 (IFI16 Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

April 6, 2012

Availability

Academic Institutions and Nonprofits only
pSW036

Plasmid

#140034

Purpose

KO of PCC 11901 acsA gene/expresses YFP under control of the cpt promoter

Depositor

Peter Nixon

Article

Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

Insert

Yellow fluorescent protein

Expression

Bacterial

Promoter

cpt

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-NT

Plasmid

#89960

Purpose

Contains arabinose-induced lambda Red and a tet-inducible non-targeting gRNA containing BsmBI sites for cloning.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

empty gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pET22-HT/plsA

Plasmid

#73625

Purpose

Produces Escherichia coli adenylate kinase (PlsA), N-terminal His6 tag

Depositor

T. J. Kappock, James Williamson

Article

Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

Insert

adenylate kinase (adk Escherichia coli str. K-12 substr. MG1655)

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

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SPLICSFAST-LDh-MT-P2A

Plasmid

#214896

Purpose

Expresses equimolar Lipid Droplet- and Mitochondria- targeted split-FAST fragments for Short-range LD-MT Contact sites

Depositor

Tito Calì

Insert

SPLICSFAST-LDh-MT-P2A

Tags

none

Expression

Bacterial and Mammalian

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pUC18-mini-Tn7

Plasmid

#64953

Purpose

mini-Tn7 base vector

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Type

Empty backbone

Expression

Bacterial

Available Since

Aug. 14, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pD434-SR rat Alpha-synuclein

Plasmid

#89076

Purpose

bacterial expression of rat Alpha-synuclein

Depositor

Michael J Fox Foundation MJFF

Insert

Alpha-synuclein (Snca Rat)

Expression

Bacterial

Promoter

T7

Available Since

March 17, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pJJS002

Plasmid

#188325

Purpose

PCR template for repair templates to tag a gene with a GFP and mIAA7 AID degron using homology-directed repair for C. elegans.

Depositor

Mike Boxem

Article

Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

Insert

Linker::GFP::mIAA7

Expression

Bacterial

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pET21a asyn(Δ41-54/Δ103-130)

Plasmid

#242236

Purpose

Expresses alpha synuclein (missing exon 3 and 5, residues 41-54 and 103-130) in bacteria

Depositor

Jennifer Lee

Article

SanGiovanni et al Protein Sci. 2025 Jul;34(7):e70195. doi:

Insert

asyn(Δ41-54/Δ103-130)

Tags

none

Expression

Bacterial

Mutation

deleted amino acids 41-54 and 103-130

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pET21a asyn(Δ103-130)

Plasmid

#242235

Purpose

Expresses alpha synuclein (missing exon 5, residues 103-130) in bacteria

Depositor

Jennifer Lee

Article

SanGiovanni et al Protein Sci. 2025 Jul;34(7):e70195. doi:

Insert

asyn(Δ103-130)

Tags

none

Expression

Bacterial

Mutation

deletion of residues 103-130

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pET21a asyn(Δ41-54)

Plasmid

#242234

Purpose

Expresses alpha synuclein (missing exon 3, residues 41-54) in bacteria

Depositor

Jennifer Lee

Article

SanGiovanni et al Protein Sci. 2025 Jul;34(7):e70195. doi:

Insert

asyn(Δ41-54)

Tags

none

Expression

Bacterial

Mutation

deleted amino acids 41-54

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pET30-T7-lacO-His-sfGFP

Plasmid

#180754

Purpose

pET30 vector with sfGFP-S-tag inserted between the Bgl II and T7 terminator

Depositor

Michael Black

Article

Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

Insert

superfolder green fluorescent protein

Tags

strep tag

Expression

Bacterial

Mutation

G140C- Please see depositor comments

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pIGc-Xph20

Plasmid

#133024

Purpose

Expresses Xph20 in bacteria

Depositor

Matthieu Sainlos

Article

Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

Insert

Xph20

Tags

His10

Expression

Bacterial

Mutation

S63K

Promoter

T7

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pUC18R6K-mini-Tn7T

Plasmid

#64958

Purpose

mini-Tn7 base vector with transcriptional terminator

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Type

Empty backbone

Expression

Bacterial

Available Since

July 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pET28a(CTLA4)

Plasmid

#177905

Purpose

Bacterial expression of his10-tagged human CTLA4 Intracellular Domain

Depositor

Ron Vale

Article

Hui et al Science. 2017 Mar 31;355(6332):1428-1433

Insert

CTLA4 (aa 183-223 only) (CTLA4 Human)

Tags

10xHis-Thrombin cut site-T7 tag

Expression

Bacterial

Promoter

T7

Available Since

May 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCB591

Plasmid

#141096

Purpose

E. coli-Lactobacilli shuttle vector for recombineering template cloning

Depositor

Chase Beisel

Article

Leenay et al Biotechnol J. 2019 Mar;14(3):e1700583. d

Type

Empty backbone

Expression

Bacterial

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pSLC-217

Plasmid

#73163

Purpose

Template plasmid which encodes a toxin gene (relE) under the control of rhamnose induceable promoter (PrhaB) to be used as a negative selection marker.

Depositor

Swaine Chen

Article

Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

Insert

PrhaB-relE (relE E. coli (bacteria))

Expression

Bacterial

Promoter

PrhaB

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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