30,260 results

1. pYTK095

   Plasmid

   #65202

   Purpose

   Encodes AmpR-ColE1 as a Type 678 part to be used in the Dueber YTK system

   Depositor

   John Dueber

   Article

   Lee et al ACS Synth Biol. 2015 Apr 14.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. NUDT3A

   Plasmid

   #42348

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   NUDT3A (NUDT3 Human)
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   See Comments

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pMCSG7-Wt-NmeCas9

   Plasmid

   #71474

   Purpose

   bacterial pMCSG7 expression vector expressing Wildtype Nme cas9 with T7 promoter, N-terminal His tag and TEV site

   Depositor

   Erik Sontheimer

   Article

   Zhang et al Mol Cell. 2015 Oct 15;60(2):242-55. doi:

   Insert

   pMCSG7-WT-NmeCas9
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCyPet-His

   Plasmid

   #14030

   Depositor

   Patrick Daugherty

   Article

   Nguyen et al Nat Biotechnol. 2005 Mar;23(3):355-60. d

   Insert

   CyPet
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 14, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. Stat2 pBluescript SK (-)

   Plasmid

   #8705

   Depositor

   Jim Darnell

   Article

   Schindler et al Proc Natl Acad Sci U S A 1992 Aug 15;89(

   Insert

   Stat2 (STAT2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 26, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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6. pML433

   Plasmid

   #177190

   Purpose

   Expresses recombinant HyPro enzyme in E. coli

   Depositor

   Eugene Makeyev

   Article

   Yap et al Mol Cell. 2021 Nov 2. pii: S1097-2765(21

   Insert

   HyPro enzyme
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJ1996_v2

   Plasmid

   #140664

   Purpose

   dCas9 & Csy4

   Depositor

   Yolanda Schaerli

   Article

   Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

   Insert

   dCas9 & Csy4
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTn7xTS_SnoopCatcher:mNG

   Plasmid

   #225191

   Purpose

   Used for inserting SnoopCatcher fused to mNeonGreen at the bacterial Tn7 site

   Depositor

   Travis Wiles

   Article

   Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

   Insert

   SnoopCatcher:mNeonGreen
   

   Use

   Tags

   SnoopCatcher

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1/V5-His-mPer1

   Plasmid

   #219421

   Purpose

   Expresses 3873 bp of mPer1 coding sequence in E. coli.

   Depositor

   Aziz Sancar

   Article

   Cao et al Proc Natl Acad Sci U S A. 2023 Oct 24;12

   Insert

   mPer1 (Per1 Mouse)
   

   Use

   Tags

   His and V5

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-28a(+)-T7-OmpA-MNaseA-6xHis

    Plasmid

    #214808

    Purpose

    Expression of 6xhis-tagged micrococcal nuclease A (MNaseA)

    Depositor

    Peter Sarin

    Article

    Gregorova et al J Biol Chem. 2024 Nov 27;301(1):108020.

    Insert

    OmpA-Micrococcal nuclease A
    

    Use

    Tags

    6xhis

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Nov. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. UMG-SP-3_pET29b

    Plasmid

    #215435

    Purpose

    Expression construct for UMG-SP-3 gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    UMG-SP-3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. NfPolyA_pET21b

    Plasmid

    #215413

    Purpose

    Expression construct for NfPolyA gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    NfPolyA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. R6K_tac_mCherry_ChInt

    Plasmid

    #187382

    Purpose

    chromosomal integration downstream of glmS gene via Tn7 system of a cassette containing IPTG-inducible mCherry

    Depositor

    Roi Avraham

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. MICU-APEX2

    Plasmid

    #79057

    Purpose

    A C-terminal APEX2 fusion to the human mitochondrial calcium uptake 1 protein MICU1

    Depositor

    Alice Ting

    Article

    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

    Insert

    C-terminal APEX2 fused MICU1 (MICU1 Human, Soybean)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV immearly promotor

    Available since

    Sept. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. p11d-sctRPA

    Plasmid

    #102614

    Purpose

    Expression of S. cerevisiea RPA in E.coli

    Depositor

    Marc Wold

    Article

    Sibenaller et al Biochemistry. 1998 Sep 8;37(36):12496-50

    Inserts

    RPA1 (RFA1 Budding Yeast)
    RPA2 (RFA2 Budding Yeast)
    RPA3 (RFA3 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    RFA2 has intron deleted

    Promoter

    T7

    Available since

    Oct. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJYS2_crtYf

    Plasmid

    #85544

    Purpose

    Constitutive transcription of crRNA of crtYf, Spectinomycin resistance

    Depositor

    Sheng Yang

    Article

    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

    Insert

    crRNA of crtYf
    

    Use

    CRISPR; Shuttle vector corynebacterium glutamicum…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. His 3C MKK3 1-347

    Plasmid

    #87878

    Purpose

    E. coli expression of MKK3 aa 1-347

    Depositor

    Peter Mace

    Insert

    MKK3 (MAP2K3 Human)
    

    Use

    Tags

    3C protease site and 6xHis

    Expression

    Bacterial

    Mutation

    aa 1-347

    Promoter

    T7

    Available since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYUBDuet

    Plasmid

    #37278

    Depositor

    Ted Baker

    Article

    Bashiri et al PLoS One. 2010 Dec 29;5(12):e15803.

    Type

    Empty backbone

    Use

    Mycobacteria shuttle vector

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAW005-2

    Plasmid

    #85611

    Purpose

    Empty gRNA delivery vector for Bacillus subtilis

    Depositor

    Perry Chou

    Article

    Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRSET YBX-1

    Plasmid

    #13035

    Depositor

    Peter Newburger

    Article

    Shen et al J Biol Chem. 1998 Mar 6. 273(10):5443-6.

    Insert

    YBX1 (YBX1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2006

    Availability

    Academic Institutions and Nonprofits only