30,249 results
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pACRISPR
Plasmid#113348PurposeA sgRNA expression plasmid for genome editing in Pseudomonas aeruginosaDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBSV2_P0026-mCherryBb
Plasmid#118239PurposeKanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes bb0026 promoter driven mCherryDepositorInsertbb0026 promoter driven mCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBBR1_DVK_AE(J23100-BCD2-E0040m-B0015)
Plasmid#197660PurposeExpresses GFP under the control of J23100 and BCD2DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
His6tev CBP HAT (1324-1700)
Plasmid#99340Purposebacterial expression of His tagged mouse CBP HAT domain (1324-1700)DepositorInsertmouse CBP HAT domain (1324-1700) (Crebbp Mouse)
UseTagsHis6tevExpressionBacterialMutationPromoterT7Available sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGLO-Pink-SHE
Plasmid#220781PurposeExpresses a raspberry chromoprotein (visible light and fluorescence) with a his-tag and an inducible GFP (araBAD-promoter)DepositorInsertraspberry chromoprotein (mRFP1)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pNIC28-SENP2
Plasmid#196355Purposeprotein expression of the His-tagged catalytic domainDepositorInsertSENP2 catalytic domain
UseTagsHis6-TEVExpressionBacterialMutationcontains only D364-L589PromoterT7Available sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-6
Plasmid#172771PurposeBacterial expression of anti-mCherry nanobody LaM-6, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-6
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-GST-CD(Cbx7)
Plasmid#82525PurposeExpresses GST-CD (Cbx7) fusion proteins in E. coli. Cbx7 chromodomain only; amino acids 1–62.DepositorInsertChromobox Homolog 7 (CBX7 Human)
UseTagsGSTExpressionBacterialMutationCD(Cbx7) ; amino acids 1–62PromoterAvailable sinceDec. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTHSSd_14
Plasmid#59952PurposeTriple split (at 67, 601-SZ)DepositorInsertThree-fragment T7* RNA polymerase
UseTagsExpressionBacterialMutationN/APromoterPTacAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBSV2_PresT-mCherryBb
Plasmid#118238PurposeKanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes resT promoter driven mCherryDepositorInsertresT promoter driven mCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLys-M1 backbone
Plasmid#109386PurposeEmpty plasmid of the pLys-M1 construct. This is medium copy (p15A), CmRDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBSV2_P0031-mCherryBb
Plasmid#118240PurposeKanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes bb0031 promoter driven mCherryDepositorInsertbb0031 promoter driven mCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
GST-CHMP4A (1-204) (pGEX2T(TEV))
Plasmid#80632PurposeResidues 1-204 of CHMP4A; Bacterial Expression vector; TEV-cleavable GST tag; Sundquist Lab internal ID: WISP06-198DepositorInsertCHMP4A (CHMP4A Human)
UseTagsGSTExpressionBacterialMutationResidues 1-204PromoterAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
p300dAIL KAT LIC 1M
Plasmid#233588PurposeBacterial expression of p300 KAT enzyme with truncated autoinhibitory loopDepositorInsertp300dAIL KAT (EP300 Human)
UseTags6xHis MBPExpressionBacterialMutationdeleted amino acids 1523-1554PromoterT7Available sinceJune 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGLO-Yellow-AZA
Plasmid#220783PurposeExpresses a yellow chromoprotein (visible light and fluorescence) with a his-tag and an inducible GFP (araBAD-promoter)DepositorInsertyellow chromoprotein
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a MDHP_YEAST
Plasmid#204233PurposeBacterial expression of yeast peroxisomal malate dehydrogenase ; Use in CURES (integration of research into undergraduate teaching labs).InsertMDHP_YEAST (MDH3 Budding Yeast)
UseTags6X HisExpressionBacterialMutationThe last 3 C-terminal residues (SKL) are the pero…PromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 Q58L wgMDH
Plasmid#204246PurposeBacterial expression of Q58L wgMDH with IPTG induction - Subunit interface mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationQ58LPromoterT5Available sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_mNeongreen-Erythropoietin
Plasmid#182422PurposeBacterial expression of mNeongreen-Erythropoietin fusionDepositorInsertmNeongreen-eythropoietin (EPO Human)
UseTagsmNeongreenExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only
