30,719 results

1. T444T

   Plasmid

   #113081

   Purpose

   Enhanced RNAi vector for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites.

   Depositor

   Tibor Vellai

   Article

   Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

   Type

   Empty backbone

   Use

   RNAi

   Expression

   Bacterial

   Available Since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28.His.3C.Tau2N4R

   Plasmid

   #226396

   Purpose

   Expresses his-tagged, 3C-cleavable WT 2N4R tau for recombinant protein production in bacteria

   Depositor

   Bradley Hyman

   Article

   Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

   Insert

   Tau2N4R (MAPT synthetic, Human)
   

   Tags

   His.3C

   Expression

   Bacterial

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUltra-5HTP

   Plasmid

   #178042

   Purpose

   Express modified ScTrpRS/tRNA for genetic incorporation of 5HTP to TAG codon

   Depositor

   Han Xiao

   Article

   Chen et al Chem. 2020 Oct 8;6(10):2717-2727. doi: 1

   Inserts

   Modified ScTrpRS for 5HTP incoporation
   suppressor tRNA for 5HTP incorporation
   

   Expression

   Bacterial

   Mutation

   T107C, P254T, C255A

   Available Since

   Jan. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC18R6KT-mini-Tn7T-Km

   Plasmid

   #64969

   Purpose

   mini-Tn7 base vector with transcriptional terminator and oriT

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Methods. 2005 Jun;2(6):443-8.

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Nov. 2, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pDEST14-Cys-SpyCatcher002

   Plasmid

   #102829

   Purpose

   Bacterial expression of Cysteine-modified SpyCatcher002 allowing reaction with thiol coupling reagents

   Depositor

   Mark Howarth

   Article

   Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

   Insert

   Cys-SpyCatcher002
   

   Tags

   GSSGS, His6, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Cysteine residue in N-terminal region

   Promoter

   T7

   Available Since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. R989-X08-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G12C

   Plasmid

   #159540

   Purpose

   E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G12C

   Depositor

   Dominic Esposito

   Article

   Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

   Insert

   His6-MBP-tev-G-Hs.KRAS4b(1-169) G12C (KRAS Human)
   

   Tags

   His6-MBP-tev

   Expression

   Bacterial

   Mutation

   G12C

   Available Since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTXTL-T7max-RLuc

   Plasmid

   #188312

   Purpose

   Expression of Renilla luciferase via a T7 promoter in TXTL

   Depositor

   Kate Adamala

   Article

   Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

   Insert

   Renilla luciferase
   

   Use

   Luciferase and Synthetic Biology

   Tags

   8x His-tag

   Expression

   Bacterial

   Promoter

   T7Max

   Available Since

   Aug. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21b FAST-PETase

   Plasmid

   #225760

   Purpose

   Expresses codon-optimized FAST-PETase in E. coli with a C-terminal his-tag

   Depositor

   Jason Boock

   Article

   Carter et al Microb Cell Fact. 2024 Nov 25;23(1):319.

   Insert

   FAST-PETase
   

   Tags

   6xHis Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. HIS-HA-CMYC P53

   Plasmid

   #233738

   Purpose

   expresses HIS-HA-CMYC triple tagged P53 for pulldown assay

   Depositor

   Albert Keung

   Article

   Han et al Methods. 2025 Mar;235:92-99. doi: 10.101

   Insert

   HIS-HA-CMYC-p53 (TP53 Human)
   

   Tags

   HIS-HA-CMYC

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET15b-His-3C-irisin

    Plasmid

    #122612

    Purpose

    Expresses in E. coli: human irisin with Nt histag and 3C protease site for cleavage

    Depositor

    Harold Erickson

    Article

    Albrecht et al Sci Rep. 2015 Mar 9;5:8889. doi: 10.1038

    Insert

    irisin (ectodomain of FNDC5) (Fndc5 Bovine, Mouse, Rat, Human)
    

    Tags

    Nt: histag-3C protease site

    Expression

    Bacterial

    Promoter

    T7 polymerase

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCURE

    Plasmid

    #23013

    Depositor

    James Bowie

    Article

    Massey-Gendel et al Protein Sci. 2009 Feb . 18(2):372-83.

    Insert

    I-CreI homing endonuclease
    

    Expression

    Bacterial

    Mutation

    I-CreI expression is controlled by the arabinose …

    Available Since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pET-DUET-1-mRhubarb719-HO

    Plasmid

    #141200

    Purpose

    Expresses mRhubarb719 fluorescent protein and heme oxygenase

    Depositor

    Elad Firnberg

    Article

    Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

    Inserts

    mRhubarb719
    heme oxygenase
    

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    R134H, Y198S, T202Y, V203I, W309R, Q310A compared…

    Promoter

    T7

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBbS8k-RFP_N99D

    Plasmid

    #109410

    Purpose

    RepA Mutant

    Depositor

    Jay Keasling

    Article

    Thompson et al Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.

    Insert

    repA
    

    Tags

    RFP

    Expression

    Bacterial

    Mutation

    N99D repA mutation to increase copy number

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBS mTSP-2

    Plasmid

    #12999

    Depositor

    Jack Lawler

    Insert

    TSP-2 (Thbs2 Mouse)
    

    Expression

    Bacterial

    Available Since

    Sept. 28, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pTW411d

    Plasmid

    #246585

    Purpose

    different combination of A. thaliana and N. tabacum Rubisco assembly factors

    Depositor

    Tina Wang

    Article

    Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

    Insert

    NtRaf1, NtRaf2, NtRbcX2, NtBSD2, AtCpn60alpha, AtCpn60beta, AtCpn20 (CPN60B, CPN60A, CPN20 Mustard Weed)
    

    Expression

    Bacterial

    Available Since

    Oct. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET30a-GABAT

    Plasmid

    #239572

    Purpose

    Expresses E. coli GABA transaminase (GABA-T/gabT) for recombinant protein purification. Contains a His6-tag on its C-terminus for Ni2+ affinity chromatography.

    Depositor

    William Plaxton

    Article

    Benidickson et al Biol Methods Protoc. 2025 Jun 20;10(1):b

    Insert

    4-aminobutyrate aminotransferase (gabT E. coli)
    

    Tags

    His6

    Expression

    Bacterial

    Available Since

    July 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYFJ84

    Plasmid

    #227644

    Purpose

    Vibrio harveyi acyl-ACP synthetase

    Depositor

    John Cronan

    Article

    Jiang et al Biochemistry. 2006 Aug 22;45(33):10008-1

    Insert

    Acyl-Acyl carrier protein synthetase
    

    Tags

    10xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDF-Leup

    Plasmid

    #213154

    Purpose

    Expresses the leupeptin protease inhibitor pathway for bolstered production of heterologously expressed proteins.

    Depositor

    Jason Crawford

    Article

    Li et al J Nat Prod. 2021 Oct 22;84(10):2692-2699

    Insert

    Leupeptin Protease Inhibitor Pathway
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pQE80l BCB

    Plasmid

    #196472

    Purpose

    Protein expression of SpyCatcher-ELP-CarHC-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher

    Depositor

    Fei Sun

    Article

    Wang et al Proc Natl Acad Sci U S A. 2017 Jun 6;114

    Insert

    BCB
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFA6a-kanMX6-P3nmt1-mECitrine

    Plasmid

    #105151

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Ye et al Mol Biol Cell. 2012 Apr;23(7):1181-95. d

    Insert

    kanMX6-P3nmt1-mECitrine
    

    Tags

    3nmt1-mECitrine

    Expression

    Bacterial and Yeast

    Mutation

    F64L, Q69M, A206K

    Promoter

    3nmt1

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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