30,416 results

1. HBT-CPK32-Flag

   Plasmid

   #107432

   Purpose

   For Arabidopsis mesophyll protoplast transient expression assay

   Depositor

   Jen Sheen

   Article

   Liu et al Nature. 2017 May 18;545(7654):311-316. d

   Insert

   CPK32 (CPK32 Mustard Weed)
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTriEx4-H6-FGAm (FlincG3)

   Plasmid

   #49202

   Purpose

   Fluorescent reporter for cGMP

   Depositor

   John Garthwaite

   Article

   Bhargava et al Front Mol Neurosci. 2013 Sep 24;6:26. do

   Insert

   FlincG3
   

   Use

   Tags

   His tag + other tags

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Met to Lys in cpEGFP region similar to CGaMP3

   Promoter

   CMV

   Available Since

   Feb. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. M13KO7-ΔPS-ΔgeneIII

   Plasmid

   #134351

   Purpose

   Helper phage for M13 phage production

   Depositor

   Mark Isalan

   Article

   Brodel et al Sci Adv. 2020 Jun 10;6(24):eaba2728. doi

   Insert

   Helper phage gene
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. BMPR2

   Plasmid

   #38972

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   BMPR2 (BMPR2 Human)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pEcNucC

   Plasmid

   #214050

   Purpose

   Bacterial expression plasmid for E. coli MS 115-1 NucC, a cA3-responsive nuclease known to cause abortive infection; used as a positive control in the Haliangium ochraceum type III CRISPR-Cas system

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   EcNucC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   lacUV5 promoter

   Available Since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-6P-1 p37deltaSEP

   Plasmid

   #113501

   Purpose

   Bacterial expression of GST-tagged p37 lacking the SEP domain

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   p37 (Ubxn2b Mouse)
   

   Use

   Tags

   GST-PreScission protease site

   Expression

   Bacterial

   Mutation

   delta 140-206

   Promoter

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTSara*SZ

   Plasmid

   #107957

   Purpose

   Arabinose-inducible split T7*RNAP with SynZIP domains 17/18

   Depositor

   Jim Haseloff

   Article

   Boehm et al Nat Commun. 2018 Feb 22;9(1):776. doi: 1

   Insert

   T7RNAP (modified)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   R632S

   Promoter

   Available Since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDC191

   Plasmid

   #115792

   Purpose

   Expresses Rat synaptobrevin 2

   Depositor

   Axel Brunger

   Article

   Lai et al Neuron. 2017 Aug 2;95(3):591-607.e10. do

   Insert

   Synaptobrevin2 (Vamp2 Rat)
   

   Use

   Tags

   hexa histidine

   Expression

   Bacterial

   Mutation

   S28C and C103S (please see comments below)

   Promoter

   T7

   Available Since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDC158

   Plasmid

   #115793

   Purpose

   Expresses Rat SNAP25

   Depositor

   Axel Brunger

   Article

   Lai et al Neuron. 2017 Aug 2;95(3):591-607.e10. do

   Insert

   SNAP25 (Snap25 Rat)
   

   Use

   Tags

   hexa histidine

   Expression

   Bacterial

   Mutation

   c84s, c85s, c90s, c92s

   Promoter

   T7

   Available Since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHO4d-Cas9

    Plasmid

    #67881

    Purpose

    Cas9nlsHis6 Bacterial Expression construct

    Depositor

    Michael Nonet

    Article

    Fu et al Nucleic Acids Res. 2014 Dec 16;42(22):13

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    His6 and T7 epitope

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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