30,260 results

1. pBSMrnaSeph

   Plasmid

   #67507

   Purpose

   Express the tRNAMet scaffold with the sephadex aptamer tag

   Depositor

   Luc Ponchon

   Article

   Ponchon et al Nat Protoc. 2009;4(6):947-59. doi: 10.10

   Insert

   modified tRNAMet with sephadex aptamer
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lpp

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pET30-GBFusion1

   Plasmid

   #133438

   Purpose

   Streptococcus GB1 domain in vector for bacterial expression.

   Depositor

   Gerhard Wagner

   Article

   Zhou et al J Biomol NMR. 2001 May . 20(1):11-4.

   Insert

   GB1 domain
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   This fragment encodes only aa 1 to 56.

   Promoter

   Available since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTn7-SCOUT21.RB

   Plasmid

   #201007

   Purpose

   pLv1-pUC18R6KT-miniTn7-Gm-mCherry-TagBFP, GmR, ApR

   Depositor

   Philip Poole

   Article

   Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

   Inserts

   mcherry
   TagBFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23104

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTn7-SCOUT11.B

   Plasmid

   #201005

   Purpose

   pLv1-pUC18R6KT-miniTn7-Gm-TagBFP, GmR, ApR

   Depositor

   Philip Poole

   Article

   Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

   Insert

   TagBFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23104

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEX6p1-hPdcd4

   Plasmid

   #20693

   Depositor

   Haiwei Song

   Article

   Loh et al EMBO J. 2009 Feb 4. 28(3):274-85.

   Insert

   Programmed Cell Death Protein 4 (PDCD4 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   I36V- does not alter function

   Promoter

   Available since

   June 9, 2009

   Availability

   Academic Institutions and Nonprofits only

6. pJBEI-6409

   Plasmid

   #47048

   Purpose

   BglBrick plasmid (=pBbA5c-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coli

   Depositor

   Taek Soon Lee

   Article

   Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

   Insert

   Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene
   

   Use

   Synthetic Biology; Bglbrick

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PlacUV5

   Available since

   Sept. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. bfloGFPa1

   Plasmid

   #60749

   Purpose

   Bacterial expression of Amphioxus GFPa1

   Depositor

   Dimitri Deheyn

   Article

   Bomati et al Sci Rep. 2014 Jun 27;4:5469. doi: 10.103

   Insert

   GFPa1
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

8. TwinStrep-SUMO-NLS-BhCas12b_v4

   Plasmid

   #158615

   Purpose

   For protein expression of BhCas12b_v4

   Depositor

   Feng Zhang

   Article

   Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

   Insert

   NLS-BhCas12b_v4
   

   Use

   Tags

   6xHIS-TwinStrep-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTW199b2

   Plasmid

   #136928

   Purpose

   Directed evolution of SpCas9

   Depositor

   David Liu

   Article

   Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

   Insert

   gIII
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAC575

    Plasmid

    #127976

    Purpose

    AMA1 plasmid with ble selection marker, and USER cassette (PacI/Nt.BbvCI)

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

    Insert

    ble (bleomycin resistance marker)
    

    Use

    Fungal expression

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Aspergillus nidulans trpC promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET30a_HIV-RT

    Plasmid

    #153311

    Purpose

    Bacterial expression of HIV reverse transcriptase

    Depositor

    Bryan Dickinson, Drew Endy

    Insert

    HIV Reverse Transcriptase
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pBAD_sfGFP3TCG4TAG

    Plasmid

    #174518

    Purpose

    Recoded sfGFP reporter with the codons TCG and TAG inserted at positions 3 and 4, respectively.

    Depositor

    Jason W Chin

    Article

    Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

    Insert

    sfGFP
    

    Use

    Tags

    6 x His

    Expression

    Bacterial

    Mutation

    Recoded. TCG and TAG codons were introduced in po…

    Promoter

    araBAD

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEP PolI

    Plasmid

    #11722

    Depositor

    Lawrence Loeb

    Article

    Camps et al Proc Natl Acad Sci U S A. 2003 Aug 19. 1

    Insert

    DNA polymerase I EP (polA E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D424A, I709N, A759R. Error-prone version of PolI.

    Promoter

    Available since

    Aug. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p15a-AsCas12f-blaZ

    Plasmid

    #171609

    Purpose

    AsCas12f1-based E. coli genome editing

    Depositor

    Quanjiang Ji

    Article

    Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

    Insert

    AsCas12f1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PJEBAN6

    Plasmid

    #203684

    Purpose

    Expresses red fluorescent marker DsRed-Express in Listeria monocytogenes

    Depositor

    Tine Rask Licht

    Article

    Andersen et al BMC Microbiol. 2006 Oct 3;6:86. doi: 10.

    Insert

    DsRed-Express
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEGFPN1-human Dynamin 1aa

    Plasmid

    #22163

    Depositor

    Pietro De Camilli

    Article

    Lee et al Proc Natl Acad Sci U S A. 2002 Jan 8. 99

    Insert

    human Dynamin 1aa (DNM1 Human)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    D744N

    Promoter

    Available since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a_His-TEV-EiCsm6

    Plasmid

    #172487

    Purpose

    Bacterial expression of codon-optimized His-EiCsm6 with a TEV protease cleavage site.

    Depositor

    Jennifer Doudna

    Article

    Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

    Insert

    Enterococcus italicus Csm6 (codon optimized)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter and lac operator

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. vGlut1 peptide- GST

    Plasmid

    #218677

    Purpose

    Expresses a GST fusion to vGlut peptide for immunoisolation of glutamatergic vesicles

    Depositor

    Axel Brunger

    Article

    Leitz et al Nat Protoc. 2024 Jul 2. doi: 10.1038/s41

    Insert

    vGlut (Slc17a6 Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTAMAHISTEV_BauA

    Plasmid

    #128952

    Purpose

    expresses BauA in E.coli

    Depositor

    James Naismith

    Article

    Moynie et al Elife. 2018 Dec 18;7. pii: 42270. doi: 1

    Insert

    ferric acinetobactin receptor
    

    Use

    Tags

    TEV protease cleavable 7xHis and TamA

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed (1-66)

    Promoter

    T7

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pXD68Kan2-AphA

    Plasmid

    #191248

    Purpose

    RSF1010-based broad host-range plasmid engineered from pAM5409, kanamycin resistance

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge