32,167 results

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mCarmine in pRSETB

Plasmid

#109484

Purpose

bacterial expression vector for mCarmine, a far red emitting fluorescent protein (excitation: 603; emission: 675)

Depositor

Oliver Griesbeck

Article

Fabritius et al Cell Chem Biol. 2018 Dec 20;25(12):1554-

Insert

mCarmine

Tags

N-terminal histidine tag in vector

Expression

Bacterial

Promoter

T7

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pMU131

Plasmid

#66811

Purpose

Shuttle vector with gram-positive origin of replication isolated from a native plasmid of Thermoanaerobacterium saccharolyticum B6A-RI

Depositor

Lee Lynd

Article

Shaw et al Appl Environ Microbiol. 2010 Jul;76(14):

Type

Empty backbone

Expression

Bacterial

Available Since

June 24, 2015

Availability

Academic Institutions and Nonprofits only

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pET-28a-hPKM2

Plasmid

#44242

Depositor

Lewis Cantley, Matthew Vander Heiden

Article

Christofk et al Nature. 2008 Mar 13;452(7184):181-6. doi

Insert

Pyruvate Kinase M2 (PKM Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

April 10, 2013

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-HygroR-T2A-TetOn3G

Plasmid

#179887

Purpose

Expresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

TetOn3G

Use

Lentiviral

Tags

Hygromycin resistance

Expression

Bacterial and Mammalian

Promoter

EF1a

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pPP085

Plasmid

#158795

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-2

Tags

6xHis

Expression

Bacterial

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pDIV272

Plasmid

#226183

Purpose

Expresses Cas9 and gRNA targeting uidA gene of the pDIV643 derived cassette.

Depositor

Uffe Mortensen

Article

Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

Insert

tRNA-sgRNA-tRNA

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

TEF1p (Komagataella phaffii)

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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M13KO7-ΔM13-ΔgeneIII-ΔgeneVI

Plasmid

#80840

Purpose

Modified helper phage

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene II
M13 gene X
M13 gene V
M13 gene VII
M13 gene IX
M13 gene VIII
M13 gene I
M13 gene XI
M13 gene IV

Expression

Bacterial

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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6His-MBP-TEV-huAsCpf1

Plasmid

#90095

Purpose

Bacterial expression plasmid for protein purification

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Insert

huAsCpf1

Tags

3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

Expression

Bacterial

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-Gm-ecfp

Plasmid

#65030

Purpose

GmR mini-Tn7 vector, mobilizable, for CFP tagging bacteria

Depositor

Herbert Schweizer

Article

Choi et al Nat Protoc. 2006;1(1):153-61.

Insert

ecfp

Expression

Bacterial

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pTetO-ISce1-TetO

Plasmid

#58506

Purpose

ISce1 restriction site is flanked by Tet operator repeats, 96 on each side

Depositor

Tom Misteli

Article

Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

Insert

Tet repeats flanking ISce1 restriction site

Expression

Bacterial

Promoter

no promoter

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEK33

Plasmid

#214945

Purpose

B. dendrobatidis plasmid for integration of target sequence at URA3 locus, hygromycin B selection

Depositor

Lillian Fritz-Laylin

Article

Brody et al Proc Natl Acad Sci U S A. 2025 Nov 4;122

Insert

BdURA3_LHA1-Hshph-G4S-cMyc-BdURA3_RHA1

Use

Bd gene targeting vector

Tags

c-Myc tag

Expression

Bacterial

Available Since

Oct. 20, 2025

Availability

Academic Institutions and Nonprofits only

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WNV isolate WN NY 2000-crow3356 NS5 RdRp full length

Plasmid

#228662

Purpose

Bacterial expression of the West Nile virus NS5 RdRp protein for biochemical assay

Depositor

ASAP Discovery, Frank von Delft

Insert

West nile NS5 RdRp

Tags

His-SUMO

Expression

Bacterial

Available Since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Pet28a_Human_H2B.1

Plasmid

#42630

Depositor

Joe Landry

Insert

Human H2B.1 (H2BC8 Human)

Tags

Untagged

Expression

Bacterial

Promoter

T7

Available Since

Feb. 22, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pHH0103_YWHAH_14-3-3

Plasmid

#109988

Purpose

Protein expression and purification of YWHAH_14-3-3

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

YWHAH_14-3-3 (YWHAH Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pQmod3C-GG

Plasmid

#191350

Purpose

Clostridium expression vector (pCB102 origin, cmR) with drop-out Plac-RFP cassette flanked with BsaI sites

Depositor

Andrew Tolonen

Article

Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

Insert

Plac-RFP (IPTG-inducible red fluorescent protein)

Expression

Bacterial

Promoter

Plac

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pORTMAGE-Pa1

Plasmid

#138475

Purpose

Enables high-efficiency recombineering in P. aeruginosa

Depositor

George Church

Article

Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

Inserts

PapRecT
mutL E36K

Expression

Bacterial

Mutation

E36K

Available Since

June 18, 2020

Availability

Academic Institutions and Nonprofits only

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pComb3

Plasmid

#63888

Purpose

For phage display of antibody libraries by fusion to modified geneIII.

Depositor

Carlos Barbas

Article

Barbas CF et al Proc Natl Acad Sci U S A. 1991 Sep 15;88

Type

Empty backbone

Use

Phage display

Tags

geneIII

Expression

Bacterial

Promoter

lacZ

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pSJ111

Plasmid

#78707

Purpose

Modular vector for constitutive secreted amylase reporter expression

Depositor

Wiep Klaas Smits

Article

Oliveira Paiva et al ACS Synth Biol. 2016 Jun 23.

Insert

amyEopt

Expression

Bacterial

Promoter

Pveg

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pL97

Plasmid

#78576

Purpose

Over-expression shuttle vector for gene cloning and transfer from E. coli to Streptomyces. Contains strong constitutive ermEp promoter

Depositor

Xuming Mao

Article

Sun et al Annals of Microbiology Dec 2012, Volume

Type

Empty backbone

Use

Streptomyces expression

Expression

Bacterial

Promoter

ermEp* promoter from S. erythraea

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pET11a-Z-NspGFP

Plasmid

#40729

Depositor

Brian McNaughton

Article

Blakeley et al Mol Biosyst. 2012 Aug;8(8):2036-40. Epub

Insert

N super positive GFP - Leucine Zipper

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Nov. 26, 2012

Availability

Academic Institutions and Nonprofits only

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