33,441 results
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Plasmid#171239PurposeMammalian expression of 1xHA-tagged human androgen receptor trancate: AR Ligand-bindind domain (LBD)DepositorInsert1xHA-tagged human androgen receptor trancate:Ligand-binding domain (AR Human)
TagsHAExpressionBacterial and MammalianMutationAR truncatePromoterCMVAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19 Giles integrase
Plasmid#170049PurposepUC19 with Giles integraseDepositorInsertGIles Integrase
ExpressionBacterialAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pM2s2AsG
Plasmid#137924PurposeE. coli Nissle pMUT2-derived plasmid with ampicillin resistance and constitutive GFP expressionDepositorInsertSuperfolder GFP
UseSynthetic Biology; For use with e. coli nissle in…ExpressionBacterialPromoterJ23101Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pExp-NusA
Plasmid#112571PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and NusA fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneTags8His and Strep tagExpressionBacterialAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLG012 (DRT type 4)
Plasmid#157890PurposeExpresses the DRT type 4 defense systemDepositorInsertDRT type 4
ExpressionBacterialAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLG029 (mzaABCDE)
Plasmid#157907PurposeExpresses the mzaABCDE defense systemDepositorInsertmzaABCDE (5-gene system containing a MutL homolog)
ExpressionBacterialAvailable sinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG034 (restriction-like)
Plasmid#157912PurposeExpresses a restriction-like defense systemDepositorInsert4-gene restriction-like system
ExpressionBacterialAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG013 (DRT type 5)
Plasmid#157891PurposeExpresses the DRT type 5 defense systemDepositorInsertDRT type 5
ExpressionBacterialAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG003 (Druantia type I)
Plasmid#157881PurposeExpresses the Druantia type I defense systemDepositorInsertDruantia type I
ExpressionBacterialAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG001 (empty vector)
Plasmid#157879PurposeEmpty vector backbone for defense systemsDepositorInsertNone
ExpressionBacterialAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLG008 (Ec78 retron)
Plasmid#157886PurposeExpresses the Ec78 retron defense systemDepositorInsertEc78 retron
ExpressionBacterialAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-A3A(N57Q)-BE3
Plasmid#158157PurposeT7 promoter bacterial expression plasmid for hAPOBEC3A(N57Q)-BE3 with N-terminal 6xHis-tag and C-terminal SV40 NLSDepositorInsert6xHis-hA3A(N57Q)-XTEN linker-hSpCas9n(D10A)-UGI-SV40NLS
Tags6xHis tag on N-terminusExpressionBacterialPromoterT7Available sinceNov. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSL1749 (pDonor, R*MmeI)
Plasmid#160733PurposeMini-transposon donor plasmid for V. cholerae CAST system. Mini-transposon has MmeI site in R end for Tn-seq. pUC19 vector backbone.DepositorInsertVchCAST donor DNA
ExpressionBacterialPromoterN/AAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
R701-X65-527: His6-tev-Hs.CALM1(2-148)
Plasmid#159693PurposeE. coli protein expression of His6-tev-Hs.CALM1(2-148)DepositorAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp16
Plasmid#156482PurposeBacterial expression of Sars-CoV2 Nsp16 protein with His-tag and GST-tagDepositorInsertNsp16 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
TagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gα11-LgB97
Plasmid#134361PurposeNanoluc complementation assay. Expression of Gα11 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gα11. Addition of the HA epitope at N terminus of Gα11.DepositorAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4092677
Plasmid#153883PurposeBacterial expression plasmid for fragment SARS-CoV-2 orf1a stop to orf1b stopDepositorInsertfragment SARS-CoV-2 orf1a stop to orf1b stop (ORF1ab Severe acute respiratory syndrome coronavirus 2)
ExpressionBacterialMutationwt;handful of SNPs as watermarksPromoterPromoter | t7 consensus from NEB, G is +1Available sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMAK464
Plasmid#140702PurposeNBαEGFPDepositorInsertNBαEGFP
ExpressionBacterialAvailable sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
[pSK131] pCOLADuet p14|MP1
Plasmid#136145Purposebacterial expression of p14 and MP1DepositorInsertp14 and MP1
ExpressionBacterialAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAT28-proD/SaEGFP
Plasmid#120321PurposeBacterial eGFP labelingDepositorInserteGFP with Staphylococcus aureus codon optimization
ExpressionBacterialPromoterproDAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only