32,870 results
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Plasmid#119782PurposeExpresses mKelly1 tagged N-terminally with an MHHHHHHGSG tagDepositorInsertmKelly1
Tags6xHis tagExpressionBacterialPromoterT7Available SinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1360
Plasmid#119278Purposefor cloning new sgRNAsDepositorInsertdcas9
ExpressionBacterialMutationD10A and H840AAvailable SinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-155
Plasmid#118537PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmScarlet-I
UseMinitn5 delivery vectorExpressionBacterialAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDAI-SceI-SacB
Plasmid#113635PurposeBroad host range replicative plasmid expressing the I-SceI homing endonuclease and the counterselectable marker SacBDepositorInsertsacB
ExpressionBacterialMutationPlease see Depositor CommentsPromotersacB promoterAvailable SinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRARE-MBP-DEST
Plasmid#84650PurposeGateway cloning vector expresses MBP-tagged proteins under the control of the IPTG– inducible ptac promoter and also expresses several tRNAs that are rare in E. coli.DepositorTypeEmpty backboneTagsMBPExpressionBacterialPromotertacAvailable SinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJL1 ATF1
Plasmid#106286PurposeExpresses ATF1 in Ecoli off a T7 promoterDepositorInsertATF1
ExpressionBacterialPromoterT7Available SinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X-1-LARP7
Plasmid#113545PurposeProduces an N-terminal GST-tagged full length LARP7 protein.DepositorAvailable SinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pACYC-GFPM2
Plasmid#102791PurposeExpresses GFP under WT T7 promoterDepositorInsertGreen Fluorescent Protein
ExpressionBacterialAvailable SinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pALOD4 non-binding mutant
Plasmid#111027PurposeExpresses non-binding mutant version of p-ALOD4, has six inactivating mutations (G501A, T502A, T503A, L504A, Y505A, and P506A)DepositorInsertDomain 4 of Anthrolysin O
TagsHis6 tagExpressionBacterialMutationMutations: S404C, C472A, G501A, T502A, T503A, L5…PromoterT7Available SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21a - Ec-ClpX
Plasmid#111516PurposeExpresses E. coli ClpX with a C-terminal His tagDepositorInsertClpX
TagsHis tagExpressionBacterialPromoterT7Available SinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFLAG_attP
Plasmid#110095PurposeVector for low-level constitutive expression in mycobacteria from the Tet promoter. Lacks the Tet repressor. Contains attP for chromosomal integration. Lacks the integrase and thus remains stably integrated in the absence of antibiotic selection. Contains a C-terminal 3xFLAG to allow detection of expression levels by Western blotting.DepositorTypeEmpty backboneTags3xFLAGExpressionBacterialAvailable SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBBR1k-J23107-GFPuv
Plasmid#106389PurposepBBR1k-GFPuv with Anderson promoter J23107 expressing lacIDepositorInsertGFPuv
ExpressionBacterialPromotertrcAvailable SinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-Lux
Plasmid#109383PurposeArabinose inducible expression of the Vibrio Fischery Lux operon. Used to test burden response in E. coliDepositorInsertpARABAD-LUX operon
ExpressionBacterialAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pD864-LacZ
Plasmid#109380PurposeRhamnose inducible LacZ expression. Used to test burden response in E. coliDepositorInsertpRHAM-LacZ
ExpressionBacterialAvailable SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ EB3-mKate2
Plasmid#105940Purposein vitro transcriptionDepositorAvailable SinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDawn-Ag43
Plasmid#107742PurposeExpresses Ag43 under the control of a blue-light transcriptional regulatorDepositorInsertAg43 (flu )
ExpressionBacterialMutationremoved all pstI cut sites with silent mutations,…PromoterpLambdaAvailable SinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAB076i3
Plasmid#79198PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI-SH2ABL1
ExpressionBacterialPromoterPlacZ-opt (OR1); Ppro1Available SinceApril 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmMaple3-CAM
Plasmid#101148PurposeFluorescent protein for superresolution imagingDepositorInsertE. coli codon optimized mMaple3
ExpressionBacterialPromoterpBADAvailable SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
HS-GFP-PACT
Plasmid#105954Purposeheat shock inducible transgenesis, PACT is a centriol markerDepositorAvailable SinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pME GFP-PCNA
Plasmid#105977Purposeentry clone for gateway cloning, cell cycle marker proliferative cell nuclear antigen PCNADepositorAvailable SinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only