30,744 results

1. pET28a-hDcp2

   Plasmid

   #72214

   Purpose

   bacterial expression of His-tagged human Dcp2

   Depositor

   Megerditch Kiledjian

   Article

   Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

   Insert

   Dcp2 (DCP2 Human)
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pGEx-4T-1-YTHDF3

   Plasmid

   #70088

   Purpose

   Encode YTHDF3 isoform a with N-terminal GST tag for E. coli expression

   Depositor

   Chuan He

   Article

   Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

   Insert

   YTH N(6)-methyladenosine RNA binding protein 3 (YTHDF3 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pHH0103_PFN1_Profilin

   Plasmid

   #110028

   Purpose

   Protein expression and purification of PFN1_Profilin

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   PFN1_Profilin (PFN1 Human)
   

   Expression

   Bacterial

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pH6HTC_HSPA9-Halo

   Plasmid

   #175332

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   HSPA9 (HSPA9 Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Available Since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pY2F

   Plasmid

   #53285

   Purpose

   Contains an Arabidopsis thaliana lcyE cDNA and produces an active lycopene epsilon-cyclase enzyme in E. coli. Use with pAC-BETA-At or pAC-BETAipi-At to produce alpha-carotene in E. coli.

   Depositor

   Francis X Cunningham Jr

   Article

   Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

   Insert

   lcyE (LUT2 Mustard Weed)
   

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   April 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGLO-Pink-SHE

   Plasmid

   #220781

   Purpose

   Expresses a raspberry chromoprotein (visible light and fluorescence) with a his-tag and an inducible GFP (araBAD-promoter)

   Depositor

   Angela Leu

   Insert

   raspberry chromoprotein (mRFP1)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   June 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBAD-LSSmOrange

   Plasmid

   #37129

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al J Am Chem Soc. 2012 May 9;134(18):7913-2

   Insert

   LSSmOrange
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

   Promoter

   araB

   Available Since

   June 5, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSL0527 (pDonor)

   Plasmid

   #130634

   Purpose

   Encodes a mini-transposon derived from V. cholerae CAST system, with CmR cargo gene. Total transposon size = 977 bp.

   Depositor

   Samuel H. Sternberg

   Article

   Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

   Insert

   VchCAST donor DNA (Mini-Tn)
   

   Use

   CRISPR; Transposon

   Expression

   Bacterial

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBTK1010 (pSL25)

   Plasmid

   #191005

   Purpose

   Expresses blue chromoprotein on a broad host range RP4 origin plasmid

   Depositor

   Jeffrey Barrick

   Article

   Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

   Insert

   blue chromoprotein
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   CP25

   Available Since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pALS3-Ma-sfGFP-WT

    Plasmid

    #212119

    Purpose

    Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.

    Depositor

    Ryan Mehl

    Inserts

    SUMO-sfGFP-WT
    M. alvus Pyl-tRNA(6)
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    araC and lpp

    Available Since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. 4xNLS-pMJ915v2

    Plasmid

    #88917

    Purpose

    For expression of modified SpyCas9 in e.coli.

    Depositor

    Jennifer Doudna

    Article

    Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Inserts

    Cas9
    T7
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-His6-SUMO-MproC145A

    Plasmid

    #172653

    Purpose

    Bacterial expression of SARS-CoV-2 Mpro C145A inactive mutant fused to SUMO

    Depositor

    Stephen Harrison

    Article

    MacDonald et al ACS Infect Dis. 2021 Sep 10;7(9):2591-25

    Insert

    SARS-CoV-2 Mpro (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    6xHis Tag and SUMO

    Expression

    Bacterial

    Mutation

    Catalytic Cysteine 145 mutated to Alanine

    Promoter

    T7

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSL0283 (pTnsABC)

    Plasmid

    #130633

    Purpose

    Expresses V. cholerae CAST TnsA, TnsB, and TnsC from a T7 promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchTnsA, VchTnsB, VchTnsC
    

    Use

    CRISPR; Transposon

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCK005.6

    Plasmid

    #153025

    Purpose

    dCas9, MCP-SoxS(R93A/S101A), J106 scRNA

    Depositor

    Jesse Zalatan

    Article

    Fontana et al Nat Commun. 2020 Apr 1;11(1):1618. doi:

    Insert

    dCas9, MCP-SoxS(R93A/S101A), J106 scRNA
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    SoxS has R93A and S101A mutations

    Available Since

    Aug. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBSTNAV

    Plasmid

    #45801

    Depositor

    Luc Ponchon

    Article

    Ponchon et al Nucleic Acids Res. 2013 Aug;41(15):e150.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Sept. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET24a-VHH-std

    Plasmid

    #109417

    Purpose

    Bacterial expression of a functionalized anti-GFP (VHH) nanobody. VHH-std also contains a T7, HA, BAP and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

    Insert

    anti-GFP nanobody fused to a T7, HA, BAP and His6 epitope
    

    Tags

    BAP, HA, His6, and T7

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. Sc RFC5-pCDFDuet

    Plasmid

    #239202

    Purpose

    Overexpress Sc RFC5 in E. coli

    Depositor

    Michael O'Donnell

    Insert

    RFC5 (RFC5 Budding Yeast)
    

    Expression

    Bacterial

    Available Since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAIP4_9C74

    Plasmid

    #234238

    Purpose

    Heterologous protein expression of variant of super folded GFP in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    9C74
    

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pEM8731

    Plasmid

    #213991

    Purpose

    PrpsM driven expression of mNeonGreen - sequence codon-optimized for Salmonella

    Depositor

    Marc Erhardt

    Article

    Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

    Insert

    mNeonGreen
    

    Expression

    Bacterial

    Promoter

    PrpsM

    Available Since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. NPY-GFP

    Plasmid

    #74629

    Purpose

    human neuropeptide Y fused to GFP

    Depositor

    Justin Taraska

    Insert

    Neuropeptide Y (NPY Human)
    

    Tags

    mEGFP

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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