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pET9d-hnRNP-A1

Plasmid

#23026

Depositor

Douglas Black

Insert

hnRNPA1 (HNRNPA1 Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 26, 2012

Availability

Academic Institutions and Nonprofits only

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pQFT-mNG

Plasmid

#203675

Purpose

pQFT with mNeonGreen under control of PQJ

Depositor

Urs Jenal

Article

Klotz et al Appl Environ Microbiol. 2023 May 18:e002

Insert

mNeonGreen

Expression

Bacterial

Promoter

PQJ

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pSET-dCas9

Plasmid

#110183

Purpose

Expression of the dCas9 gene in Streptomyces

Depositor

Yinhua Lu

Article

Zhao et al Biotechnol J. 2018 Jun 3. doi: 10.1002/b

Insert

dCas9

Use

CRISPR; I

Expression

Bacterial

Mutation

D10A, H840A

Promoter

ermE*p

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pC0073 EiCsm6 His6-TwinStrep-SUMO-BsaI

Plasmid

#115268

Purpose

Expresses EiCsm6 from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

EiCsm6

Tags

SUMO

Expression

Bacterial

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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p406TDH3

Plasmid

#15977

Depositor

Nicolas Buchler, Fred Cross

Insert

TDH3 promoter + polylinker + CYC1 terminator (TDH3 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

TDH3 promoter is between SacI and XbaI restrictio…

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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ILF2_DZF_pGEX

Plasmid

#135162

Purpose

Recombinant expression of ILF2 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

ILF2 (ILF2 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

Nov. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pCIRCNpu

Plasmid

#74226

Purpose

Vector for SICLOPPS-mediated protein circularization using the Nostoc punctiforme DnaE intein.

Depositor

Barbara Di Ventura, Roland Eils

Article

Waldhauer et al Mol Biosyst. 2015 Nov 10;11(12):3231-43.

Type

Empty backbone

Use

Synthetic Biology

Tags

Npu DnaE C-intein and Npu DnaE N-intein

Expression

Bacterial

Promoter

T7-lac

Available Since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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pOPINK-TRABID (NZF1, aa 1-33)

Plasmid

#65437

Purpose

Expresses K29-/K33-specific TRABID NZF1 (aa 1-33) in E. coli

Depositor

David Komander

Article

Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

Insert

TRABID (ZRANB1 Human)

Tags

His6-GST

Expression

Bacterial

Mutation

only codes for amino acids 1-33 of TRABID, which …

Promoter

T7

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
pRSET FLIPPi-200u

Plasmid

#13555

Depositor

Wolf Frommer

Article

Gu et al FEBS Lett. 2006 Oct 30. 580(25):5885-93.

Insert

FLIPPi G162A

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

G162A in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-FGF2-K128N

Plasmid

#120284

Purpose

Produce human recombinant FGF2 K128N in DE3 cells

Depositor

Paul Burridge

Article

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

Basic fibroblast growth factor 2 (FGF2 Human, Synthetic)

Tags

6x His

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 promoter

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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KARS_pNIC-Bio3

Plasmid

#153051

Purpose

Expression construct for producing Avi tagged Lysyl-tRNA synthetase

Depositor

Susanne Gräslund

Insert

KARS (KARS1 Human)

Tags

Avi and His6-TEV

Expression

Bacterial

Promoter

T7

Available Since

June 29, 2020

Availability

Academic Institutions and Nonprofits only

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pOPINK-TRABID (OTU+AnkUBD, aa 245-697)

Plasmid

#61590

Purpose

Expresses human TRABID (OTU+AnkUBD domains) in E. coli.

Depositor

David Komander

Article

Licchesi et al Nat Struct Mol Biol. 2011 Dec 11;19(1):6

Insert

TRABID (ZRANB1 Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-244 and aa 698-708.

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a_Pro-IL-33

Plasmid

#214342

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL33 (IL33 Human)

Tags

His-TEV and Myc

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET30a(+)-PE2

Plasmid

#180015

Purpose

Expressing PE2 protein in E.coli for protein purification

Depositor

Dirk Hockemeyer

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

PE2

Tags

6xHis

Expression

Bacterial

Promoter

Lac operator-controlled T7 promoter

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pGDP3 aph(3'')-Ia

Plasmid

#112879

Purpose

This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

Depositor

Gerard Wright

Article

Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

Insert

aph(3'')-Ia (SGR_RS01140 Streptomyces griseus)

Tags

6xHis

Expression

Bacterial

Promoter

Pbla

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pSLS.400

Plasmid

#187201

Purpose

Expresses retron Eco4 RT

Depositor

Seth Shipman

Article

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Insert

Retron Eco4 Reverse Transcriptase

Use

Synthetic Biology

Expression

Bacterial

Promoter

mphR

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pVRa28_1088

Plasmid

#49683

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF28_1088

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available Since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pEN-sacB

Plasmid

#196249

Purpose

Plasmid containing the sacB gene

Depositor

Bing Yang

Article

Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

Insert

attR2-sacB-attL3

Expression

Bacterial

Promoter

sacB promoter

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMyBADNT-kan

Plasmid

#84691

Purpose

Protein expression in mycobacteria

Depositor

Matthias Wilmanns

Article

Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

Type

Empty backbone

Tags

His6

Expression

Bacterial

Promoter

AraC

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pInt

Plasmid

#18941

Depositor

Michele Calos

Article

Groth et al Proc Natl Acad Sci U S A. 2000 May 23. 9

Insert

phiC31 integrase (int Streptomyces phage phiC31)

Expression

Bacterial

Mutation

under lacZ promoter

Available Since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only

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