33,906 results
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Plasmid#182763PurposepH-sensitive RFP pHlorina for detection of efferocytosisDepositorInsertpHlorina
ExpressionBacterialAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
SBFP2-anti-AlfaTag nanobody
Plasmid#171826PurposeBacterial expression vector for production of fluorescent anti-AlfaTag nanobodyDepositorInsertSBFP2-anti-AlfaTag nanobody
Tags6xHis and SEP tag (6xD)ExpressionBacterialAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-EIN-FTO(32-505)
Plasmid#186466PurposeExpresses human RNA demythelase protein FTO 32-505 amino acid sequence with N-terminal fusion with the N-domain of EI ( e.coli protein)DepositorInsertFat mass obesity associated protein (FTO Human)
Tags6 Histidine-EIN (N-terminal domain of E.coli Enzy…ExpressionBacterialMutationamino acid 32-505PromoterT7Available sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
SpyTag-sfGFP
Plasmid#184226PurposeExpresses SpyTag-superfolder GFP in the bacterial cytoplasm. SpyTag enables covalent reaction with SpyCatcher.DepositorInsertSpyTag-sfGFP
TagsHis6, SpyTag, and TEV protease cleavage siteExpressionBacterialPromoterT5Available sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKAR2-Br512_Mut235
Plasmid#179278PurposeExpresses Br512_Mut235 polymerase in E.coliDepositorInsertEngineered Bst DNA Polymerase I Large Fragment
Tags8x His tag; Villin Headpiece 47 amino acid (HP47)…ExpressionBacterialPromoterT7Available sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWM_12x601_15bpLinker
Plasmid#157786PurposeEcoRV digestion produces 12x601 chromatin assembly construct with 15 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomesDepositorInsert12x601_15bp linker
ExpressionBacterialAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p15a-AsCas12f-blaZ
Plasmid#171609PurposeAsCas12f1-based E. coli genome editingDepositorInsertAsCas12f1
ExpressionBacterialAvailable sinceDec. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT/6xHis mCherry F30 2xdBroccoli
Plasmid#169233PurposeConstitutive T7 RNAP-mediated expression of 6xHis-mCherry with 2xd Broccoli RNA aptamer.DepositorInsert6xHis mCherry F30-2xdBroccoli
ExpressionBacterialPromoterT7 promoterAvailable sinceDec. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCE38
Plasmid#174432PurposeC. auris LEUpOUT HYG marker CAS9 expression construct. Use with pCE41 gRNA expression construct.DepositorInsertC. auris LEU2 1 of 2, pENO1, Cas9, HYG 1 of 2
UseCRISPRExpressionBacterialAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCE41
Plasmid#174433PurposeC. auris LEUpOUT HYG marker gRNA expression construct. Use with pCE38 CAS9 expression construct.DepositorInsertHYG 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2
UseCRISPRExpressionBacterial and YeastAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_HaloTag9
Plasmid#169324PurposeExpression of HaloTag9 in bacterial cellsDepositorInsertHaloTag9
TagsHisExpressionBacterialMutationHaloTag9 = HaloTag7-Q165H-P174RAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET_His6-SUMO-TEV-TtCsm6
Plasmid#172486PurposeBacterial expression of codon-optimized His-SUMO-TtCsm6 with a TEV protease cleavage site.DepositorInsertT. thermophilus Csm6 (codon optimized) (csm6 Synthetic)
TagsHis-SUMO-TEVExpressionBacterialPromoterT7 promoter and lac operatorAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPSU100
Plasmid#173835PurposeExpresses 100 kD Penn State ladder proteinDepositorInsertSTRHSTPABPACIL1bQRS
ExpressionBacterialPromoterT7Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKAR2-Br512g3.1
Plasmid#171200PurposeExpresses Br512g3.1 polymerase in E.coliDepositorPublicationInsertEngineered Bst DNA Polymerase I Large Fragment
Tags8x His tag; Villin Headpiece 47 amino acid (vHP47…ExpressionBacterialPromoterT7Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-sfGFP-DogTag Loop A
Plasmid#171779PurposeExpresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Val22 and Asn23DepositorInsertsfGFP-DogTag Loop A
TagsHis6ExpressionBacterialMutationDogTag and linkers inserted between residues Val2…PromoterT7Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21b-RBD-JA1
Plasmid#167126PurposeExpresses SARS-CoV-2 RBD in bacterial systemDepositorInsertSARS-CoV-2 Receptor Binding domain (S SARS-CoV-2)
ExpressionBacterialMutationresidues (333 to 529 aa)PromoterT7Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pK27Sumo_His-SUMO-nsp10-nsp14 fusion (SARS-CoV-2)
Plasmid#169161PurposeTo express nsp10-nsp14 fusion protein in E. coliDepositorInsert14His-SUMO-nsp10-GGSGGS-nsp14 (ORF1ab SARS-CoV-2, Synthetic)
Tags14His-SUMO and Fusion protein of SARS-CoV-2 nsp10…ExpressionBacterialMutationCodon optimised for E. coliPromoterT5Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-26b-Nb127D01-HA-His
Plasmid#171566PurposeBacterial expression of HA-His-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-HA-His)DepositorPublicationInsertNb127D01-HA-His (CXCR2 Human)
TagsHA-tag/His-tag and PelB signal peptideExpressionBacterialAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTarget12f1
Plasmid#171183PurposeConstitutive expression of sgRNA without donor DNADepositorInsertsgRNA scaffold in the CRISPR/Cas12f1 system
UseCRISPRExpressionBacterialPromoterJ23119(SpeI) promoterAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-AR-LBD
Plasmid#171239PurposeMammalian expression of 1xHA-tagged human androgen receptor trancate: AR Ligand-bindind domain (LBD)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert1xHA-tagged human androgen receptor trancate:Ligand-binding domain (AR Human)
TagsHAExpressionBacterial and MammalianMutationAR truncatePromoterCMVAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only