30,260 results

1. EV-A71 2A protease; 6xHis-MBP-TEV tag, self cleaving

   Plasmid

   #204811

   Purpose

   Bacterial expresison of Enterovirus A71 2A protease

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   A71 2A protease
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 16, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. BPK2014-Cas12i-Max

   Plasmid

   #188276

   Purpose

   prokaryotic expression

   Depositor

   Wei Li

   Article

   Chen et al Innovation (Camb). 2022 May 26;3(4):1002

   Insert

   Cas12i-Max
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7

   Available since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBb(RK2)1k-GFPuv

   Plasmid

   #106394

   Purpose

   IPTG-inducible trc promoter expressing gfpuv on RK2 origin

   Depositor

   Brian Pfleger

   Article

   Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

   Insert

   GFPuv
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   trc

   Available since

   March 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMZ0002

   Plasmid

   #102239

   Purpose

   Expresses Chinese hamster NSF in bacteria

   Depositor

   Axel Brunger

   Article

   Zhao et al Nature. 2015 Feb 5;518(7537):61-7. doi:

   Insert

   N-ethylmaleimide sensitive factor
   

   Use

   Tags

   6x His tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBECKP-km

   Plasmid

   #117235

   Purpose

   A cytidine deaminase-mediated base-editing plasmid in Klebsiella Pneumoniae

   Depositor

   Quanjiang Ji

   Article

   Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

   Insert

   ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQE81XN-mGold

   Plasmid

   #218959

   Purpose

   Expresses Hisx6-Tagged mGold in bacteria

   Depositor

   Benjamin Glick

   Insert

   mGold
   

   Use

   Tags

   6X-His

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGST-DHFR Mm

   Plasmid

   #83073

   Purpose

   Expresses the full length DHFR Mm in E.coli

   Depositor

   Gali Prag

   Article

   Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

   Insert

   2x DHFR (Dhfr Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSETb-pcDronpa2-A69T

   Plasmid

   #98578

   Purpose

   photoconvertible fluorescent protein pcDronpa2-A69T in pRSetB plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression

   Depositor

   Ulrike Endesfelder

   Article

   Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

   Insert

   pcDronpa2-A69T
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pZ8-Ptac

   Plasmid

   #74064

   Purpose

   IPTG inducible version of pZ8-1 plasmid, to which lacIq was added, KanR

   Depositor

   Timothy Lu

   Article

   Cleto et al ACS Synth Biol. 2016 Feb 16.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   ptac

   Available since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMD19-PBBa_J23117-sgRNA-Spr

    Plasmid

    #190793

    Purpose

    Template vector to amplify single sgRNAs or pieces for multiplexing arrays. Has flanking BsaI-sites.

    Depositor

    Paul Hudson

    Article

    Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

    Insert

    sgRNA (dummy)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23117

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBad-UXPS

    Plasmid

    #172138

    Purpose

    bacteria expression plasmid for a UDP/UTP sensor

    Depositor

    Huiwang Ai

    Article

    Li et al ACS Cent Sci. 2021 Oct 27;7(10):1763-177

    Insert

    UXPS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDuet TDP-43 Q331K

    Plasmid

    #27465

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Coexpression

    Tags

    Expression

    Bacterial

    Mutation

    Glutamine 331 mutated to Lysine (Q331K)

    Promoter

    Available since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pST5552

    Plasmid

    #36255

    Purpose

    Mycobacteria replicating vector expressing eGFP.

    Depositor

    Carolyn Bertozzi, Jessica Seeliger

    Article

    Seeliger et al PLoS One. 2012;7(1):e29266. Epub 2012 Ja

    Insert

    eGFP
    

    Use

    Synthetic Biology; Mycobacteria replicating vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ModifiedHsp60

    Available since

    May 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pST50Trc1-HISNDHFR

    Plasmid

    #63940

    Purpose

    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Use

    Tags

    6xHis; TEV cleavable

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMycoFos

    Plasmid

    #84577

    Purpose

    Shuttle and expression vector. Replicates in E.coli and Mycobacterium. Gives acetamide-inducible gene expression in Mycobacterium. Copy number is controllable by arabinose in E.coli.

    Depositor

    Nicholas Coleman

    Article

    Ly et al J Microbiol Methods. 2011 Sep;86(3):320-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 7, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pGEX-6p1-GST-ArcFL

    Plasmid

    #119877

    Purpose

    Bacterial Expression construct for protein production and purification

    Depositor

    Jason Shepherd

    Article

    Pastuzyn et al Cell. 2018 Mar 22;173(1):275. doi: 10.10

    Insert

    Activity Regulated Cytoskeletal Protein (Arc Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET21-Flavidin

    Plasmid

    #89881

    Purpose

    Fluorophore-friendly streptavidin that can improve fluorescent brightness and has unimpaired biotin binding after dye labeling

    Depositor

    Mark Howarth

    Article

    Jacobsen et al Cell Chem Biol. 2017 Jul 17. pii: S2451-

    Insert

    Streptavidin-K80R-K121R-K132R-K134R-Glutamate_Tag
    

    Use

    Tags

    6 glutamate tag (C terminal on insert)

    Expression

    Bacterial

    Mutation

    K80R, K121R, K132R, K134R

    Promoter

    Available since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTn7-SCOUT11.Y

    Plasmid

    #201004

    Purpose

    pLv1-pUC18R6KT-miniTn7-Gm-sYFP2, GmR, ApR

    Depositor

    Philip Poole

    Article

    Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

    Insert

    sYFP2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23100

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHH0103_VASP_WH1

    Plasmid

    #109790

    Purpose

    Protein expression and purification of VASP_WH1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    VASP_WH1 (VASP Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYTK004

    Plasmid

    #65111

    Purpose

    Encodes ConL2 as a Type 1 part to be used in the Dueber YTK system

    Depositor

    John Dueber

    Article

    Lee et al ACS Synth Biol. 2015 Apr 14.

    Insert

    ConL2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge