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pRetroX-PTuner DD-linker-M.EcoGII

Plasmid

#122082

Purpose

Express M.EcoGII with detabilization domain - inducible

Depositor

Laure Crabbe

Article

Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

Insert

DD-linker-M.EcoGII

Use

Retroviral

Tags

V5 tag on Nter of M.EcoGII

Expression

Bacterial and Mammalian

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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pACYCDuet-1:ChrA

Plasmid

#225079

Purpose

Expresses precursor peptide ChrA in E.coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

Insert

ChrA

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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PB-TRE3G-MYCN

Plasmid

#104542

Purpose

PiggyBac vector encoding doxycycline-inducible human MYCN

Depositor

David Vereide

Article

Miller et al Stem Cell Reports. 2018 Jan 9;10(1):73-8

Insert

MYCN proto-oncogene, bHLH transcription factor (MYCN Human)

Expression

Bacterial and Mammalian

Promoter

TRE3G

Available Since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pHis-BRD4 BD1

Plasmid

#196544

Purpose

Expression of BRD4 Bromodomain (BD1) in E.coli

Depositor

Byung Il Lee

Article

Kim et al Sci Rep. 2023 Jul 4;13(1):10805. doi: 10

Insert

BRD4 BD1 (BRD4 Human)

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-GST-His6-1_HBD

Plasmid

#171869

Purpose

Expresses a specific DNA-RNA hybrid sensor protein with the HBD domain of RNase H1 in bacterial cells

Depositor

Kaiwei Liang

Article

Wang et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabe

Insert

Hybrid binding domain of Rnase H1

Tags

GST- and His10-tag

Expression

Bacterial

Mutation

WT

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pMyC

Plasmid

#42192

Depositor

Annabel Parret, Matthias Wilmanns

Article

Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

Type

Empty backbone

Tags

His6

Expression

Bacterial

Promoter

ColE1

Available Since

Aug. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pET15TV-L-AldA WT

Plasmid

#187976

Purpose

Expresses human aldolase A

Depositor

Audrey Lamb

Article

Fenton et al Protein Sci. 2022 Feb;31(2):357-370. doi

Insert

AldA WT (ALDOA Human)

Tags

Hexahistag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pnsgRNA

Plasmid

#172717

Purpose

Delivery of guide RNA for prime editing

Depositor

Tilmann Weber

Article

Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

Insert

J23119-gRNA

Expression

Bacterial

Available Since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pRha-ABE8e-NRCH

Plasmid

#165417

Purpose

For bacterial purification of ABE8e-NRCH protein

Depositor

David Liu

Insert

ABE8e-NRCH

Tags

8xHIS and BP-NLS

Expression

Bacterial

Promoter

pRhaBAD

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pUCP20T-eyfp

Plasmid

#78466

Purpose

Broad range bacterial fluorescence labeling

Depositor

Mariette Barbier

Article

Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

Insert

eYFP

Expression

Bacterial

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pdCas12f1-LVA

Plasmid

#220993

Purpose

Prokaryotic and constitutive expression of AsCas12f-LVA

Depositor

John van der Oost

Article

Swartjes et al iScience. 2025 Nov 12;28(12):114033. doi

Insert

Acidibacillus sulfuroxidans Cas12f1

Use

CRISPR

Tags

LVA

Expression

Bacterial

Mutation

D225A

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pSC101-MP-mRFP1

Plasmid

#240204

Purpose

Low copy mRFP1 reporter for monitoring metabolite-responsive CRISPRa activity

Depositor

Wilfred Chen

Article

Stohr et al Nucleic Acids Res. 2025 Nov 13;53(21):gk

Insert

mRFP1

Expression

Bacterial

Promoter

J3-DA9-UP1-MP1

Available Since

Nov. 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pLK06Hpp-Fab CR3009

Plasmid

#239861

Purpose

Plasmid for simultaneous Fab and GFP expression from separate Lac promoters for online monitoring of induction with a GFP sensor station. Fab is expressed as a fusion with alkaline phosphatase.

Depositor

Urpo Lamminmäki

Article

Korkiakoski et al Protein Expr Purif. 2025 Aug 28:106808.

Inserts

Fab (antigen binding fragment) anti-nucleocapsid protein of SARS-COV viruses 1 and 2 (N, N Human)
GFP with F64L, S65T, Q80R, M153T and V163A mutations

Tags

Hexahistidine tag and bacterial alkaline phosphat…

Expression

Bacterial

Mutation

F64L, S65T, Q80R, M153T and V163A

Promoter

Lac promoter

Available Since

Sept. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pMS10

Plasmid

#230074

Purpose

Constitutive expression Promoter (RpoD sigma factor, BT_RS06635), integrates via IntN1; N-/C-terminal mNeonGreen fusion, ermF, C-terminal 3x-FLAG, R6K ori, RP4 oriT

Depositor

Sean Crosson

Article

Schnizlein et al Microbiol Resour Announc. 2025 May 8;14(

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG and mNeonGreen

Expression

Bacterial

Promoter

RpoD (bt_1311/BT_RS06635)

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pMS02

Plasmid

#230066

Purpose

Constitutive expression Promoter (RpoD sigma factor, BT_RS06635), integrates via IntN1; ermF, C-terminal 3x-FLAG, R6K ori, RP4 oriT

Depositor

Sean Crosson

Article

Schnizlein et al Microbiol Resour Announc. 2025 May 8;14(

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Expression

Bacterial

Promoter

RpoD (bt_1311/BT_RS06635)

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pGFP-PacI

Plasmid

#170100

Purpose

deGFP reporter plasmid with unique PacI restriction enzyme recognition site

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

deGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

p70a

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pMM575

Plasmid

#68881

Purpose

NanoLuc expressed constiutively from PcfiA, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

NanoLuc

Expression

Bacterial

Available Since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only

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Psy074

Plasmid

#99504

Purpose

Bacterial expression of LIPS M1+TE

Depositor

Jay Keasling

Article

Yuzawa et al ACS Synth Biol. 2017 Jan 20;6(1):139-147

Insert

LIPS M1+TE

Expression

Bacterial

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pN006

Plasmid

#188470

Purpose

Lentiviral construct encoding doxycycline-inducible AsCas12a (via rtTA3)

Depositor

Le Cong

Article

Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

Insert

AsCas12a

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

Sept. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pTcI-Wasabi

Plasmid

#86101

Purpose

mWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TcI
mWasabi

Tags

6xHis

Expression

Bacterial

Mutation

A67T

Promoter

pR/pL and pR/pL, LacI

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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