30,261 results

1. pJK556

   Plasmid

   #74277

   Purpose

   Produces formylglycinamide ribonucleotide amidotransferase, N-terminal His6 tag (H6EcPurL)

   Depositor

   T. J. Kappock

   Insert

   formylglycinamidine ribonucleotide synthetase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   changes alanine-1246 to serine, numerous silent m…

   Promoter

   T7

   Available since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET14b – E. coli Exonuclease I

   Plasmid

   #104552

   Purpose

   bacterial expression of Exonuclease I

   Depositor

   Charles Bell

   Article

   Korada et al Nucleic Acids Res. 2013 Jun;41(11):5887-

   Insert

   Exonuclease I (sbcB E.coli)
   

   Use

   Tags

   6X-His tag followed by thrombin site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBbS2c-RFP

   Plasmid

   #35329

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   RFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Tet

   Available since

   Feb. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMW41: pET-His-MBP-SUMO-R2Bm-SII

   Plasmid

   #209290

   Purpose

   Expresses the R2Bm ORF in E. coli

   Depositor

   Feng Zhang

   Article

   Wilkinson et al Science. 2023 Apr 21;380(6642):301-308.

   Insert

   R2
   

   Use

   Tags

   His, MBP, StrepII, and bdSUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pC0073 EiCsm6 His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115268

   Purpose

   Expresses EiCsm6 from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   EiCsm6
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBbS6k-RFP

   Plasmid

   #35294

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   RFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   LlacO-1

   Available since

   Feb. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGR596

   Plasmid

   #213787

   Purpose

   Integrates at the CAN1 locus the BadBoy3 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette

   Depositor

   Chang Liu

   Insert

   BadBoy2 TP-DNAP1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   See manuscript

   Promoter

   Available since

   March 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEXtev-HMG54kd

   Plasmid

   #26030

   Depositor

   Johann Deisenhofer

   Article

   Istvan et al EMBO J. 2000 Mar 1. 19(5):819-30.

   Insert

   3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Catalytic domain (amino acids 426-488) Methion…

   Promoter

   Available since

   Dec. 3, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC19-T7-3WJdB-T

   Plasmid

   #87308

   Purpose

   Expresses the fluorescence-activating Three-way Junction dimeric Broccoli (3WJdB) aptamer under the control of the T7 promoter.

   Depositor

   Donald Burke

   Article

   Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

   Insert

   Three-way junction dimeric Broccoli aptamer
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET-His-VCre

    Plasmid

    #123131

    Purpose

    Bacterial expression plasmid for wild-type VCre with an N-terminal His-tag.

    Depositor

    David Liu

    Article

    Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

    Insert

    VCre recombinase
    

    Use

    Cre/Lox

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pT7-7 asyn E46K

    Plasmid

    #105728

    Purpose

    Bacterial expression of mutant human alpha synuclein

    Depositor

    Hilal Lashuel

    Article

    Mbefo et al J Biol Chem. 2015 Apr 10;290(15):9412-27

    Insert

    alpha-synuclein E46K (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E46K

    Promoter

    T7

    Available since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIntegrase_8

    Plasmid

    #60579

    Purpose

    plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Integrase_8
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pBAD

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a 6xHis PreScission SARS-CoV-2 nsp13

    Plasmid

    #159390

    Purpose

    Expresses the SARS-CoV-2 nsp13 protein with an N-terminal His6 tag followed by an HRV 3C/PreScission cleavage site.

    Depositor

    Tarun Kapoor

    Article

    Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

    Insert

    N-terminal His tag PreScission SARS-CoV-2 nsp13, optimized for insect cell expression
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. APneg-VAMP1b

    Plasmid

    #168008

    Purpose

    negative selection PACE Accessory Plasmid

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    VAMP1(T29-K89)-T7PAP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. APneg-VAMP1c

    Plasmid

    #168009

    Purpose

    negative selection PACE Accessory Plasmid

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    VAMP1(T29-K89)-T7PAP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SIRT7.4

    Plasmid

    #13740

    Depositor

    John Denu

    Article

    Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

    Insert

    SIRT7 (SIRT7 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 23, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pNDGG003

    Plasmid

    #231316

    Purpose

    BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RK2-Tc-par-ELT4; GG assembly from pOGG010, pOGG012, pOGG014, pOGG042, and pOGG004, TcR

    Depositor

    Barney Geddes

    Article

    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. ARHGEF2A-c055

    Plasmid

    #205103

    Purpose

    ARHGEF2 protein expression

    Depositor

    Paul Brennan

    Insert

    ARHGEF2 (ARHGEF2 Human)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ARHGEF2A-c052

    Plasmid

    #205102

    Purpose

    ARHGEF2 protein expression

    Depositor

    Paul Brennan

    Insert

    ARHGEF2 (ARHGEF2 Human)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. RHOAA-c035

    Plasmid

    #205118

    Purpose

    RHOA protein expression

    Depositor

    Paul Brennan

    Insert

    RHOA (RHOA Human)
    

    Use

    Tags

    6His-TEV and AviTag

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge