30,744 results

1. pNCST-AvicFP3

   Plasmid

   #129506

   Purpose

   Expresses AvicFP3 constitutively in E. coli (most strains)

   Depositor

   Nathan Shaner

   Article

   Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

   Insert

   AvicFP3
   

   Expression

   Bacterial

   Promoter

   synthetic constitutive (stationary phase) promote…

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJUMP49-2D'(sfGFP)

   Plasmid

   #126994

   Purpose

   Level 2 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   None

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJUMP29-1D'(sfGFP)

   Plasmid

   #126981

   Purpose

   Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   None

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETM6-G6-vioABE

   Plasmid

   #73438

   Purpose

   Prodeoxyviolacein pathway (vioABE) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variant G6.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   PG6-vioABE
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   PG6 (orthogonal T7-lac variant)

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDx_mScarlet-I3-SYFP2

   Plasmid

   #189763

   Purpose

   Dual expression vector for bacteria and mammalian cells producing mScarlet-I3-SYFP2 fusion protein for FRET analysis

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   mScarlet-I3-SYFP2
   

   Tags

   6xHis

   Expression

   Bacterial and Mammalian

   Promoter

   CMV & Rha

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-28a(+)-FGF1-4X

   Plasmid

   #120282

   Purpose

   Produce human recombinant FGF1-4X in DE3 cells

   Depositor

   Paul Burridge

   Article

   Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

   Insert

   Basic fibroblast growth factor 1 mutant
   

   Tags

   6x His tag

   Expression

   Bacterial

   Mutation

   Codon optimized for E. Coli production

   Promoter

   T7 Promoter

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHEN-CoV2-nanobody-F1

   Plasmid

   #194592

   Purpose

   Bacterial expression of F1 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

   Depositor

   Gerald McInerney

   Article

   Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

   Insert

   F1 (S )
   

   Expression

   Bacterial

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pVoPo-01

   Plasmid

   #187836

   Purpose

   Backbone for transcriptional reporter system in Fusobacterium nucleatum

   Depositor

   Jörg Vogel

   Article

   Ponath et al Proc Natl Acad Sci U S A. 2022 Oct 4;119

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX4T-1-Optimized E6AP

   Plasmid

   #165100

   Purpose

   ecoli optimized expression of GST-fusion of human E6AP

   Depositor

   Arthur Haas

   Article

   Ronchi et al J Biol Chem. 2013 Apr 12;288(15):10349-6

   Insert

   E6AP isoform 3 (UBE3A Human)
   

   Tags

   GST from plasmid.

   Expression

   Bacterial

   Mutation

   codon optimized for E. coli expression

   Available Since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYES1L-URA

    Plasmid

    #84301

    Purpose

    E.coli shuttle vector for large-scale DNA assembly in S. cerevisiae.

    Depositor

    George Church

    Article

    Ostrov et al Science. 2016 Aug 19;353(6301):819-22. d

    Type

    Empty backbone

    Expression

    Bacterial and Yeast

    Available Since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBR BsmAIM

    Plasmid

    #206206

    Purpose

    Constitutive expression of BsmAIM methyltransferase

    Depositor

    Andrew Ellington

    Article

    Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

    Insert

    BsamAIM
    

    Expression

    Bacterial

    Available Since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHEN-CoV2-nanobody-E2

    Plasmid

    #194590

    Purpose

    Bacterial expression of E2 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

    Depositor

    Gerald McInerney

    Article

    Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

    Insert

    E2 (S )
    

    Expression

    Bacterial

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. nifF in pET21b

    Plasmid

    #124520

    Purpose

    Azotobacter vinelandii flavodoxin II of Nitrogenase

    Depositor

    Douglas Rees

    Article

    Segal et al Protein Sci. 2017 Oct;26(10):1984-1993.

    Insert

    Flavodoxin II (nifF Azotobacter vinelandii)
    

    Tags

    None

    Expression

    Bacterial

    Promoter

    T7 / lac

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSF-CjPglB

    Plasmid

    #199305

    Purpose

    pSN18 derivative encoding C.jejuni PglB with C-terminal FLAG epitope tag

    Depositor

    Michael Jewett

    Article

    Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

    Insert

    CjPglB with flag tag
    

    Tags

    Flag tag

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSMART-SOS-GFPuv

    Plasmid

    #102283

    Purpose

    Fluorescent sensor of E. coli SOS response

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    cda-GFPuv
    

    Expression

    Bacterial

    Promoter

    cda SOS promoter

    Available Since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEB1-mGFPmut2

    Plasmid

    #103980

    Purpose

    Plasmid encoding mGFPmut2

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mGFPmut2
    

    Use

    Low copy

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (S65A, V68L, …

    Promoter

    proC

    Available Since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDEST17-UbE2B

    Plasmid

    #15781

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2B (UBE2B Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pPagP

    Plasmid

    #112125

    Purpose

    Bacterial expression of outer membrane protein PagP, wild type

    Depositor

    Karen Fleming

    Article

    Burgess et al J Biol Chem. 2008 Sep 26;283(39):26748-5

    Insert

    PagP (pagP E. coli)
    

    Expression

    Bacterial

    Mutation

    N-terminal signal sequence not included and repla…

    Promoter

    T7

    Available Since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pALOD4

    Plasmid

    #111026

    Purpose

    Expresses domain 4 of anthrolysin O cholesterol sensor, has N-terminal His6-tag and two point mutations, S404C and C472A

    Depositor

    Arun Radhakrishnan

    Article

    Gay et al Biophys J. 2015 Mar 24;108(6):1459-69. d

    Insert

    Domain 4 of Anthrolysin O
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Mutations: S404C, C472A

    Promoter

    T7

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHYRS52

    Plasmid

    #31122

    Depositor

    Hideo Iwai

    Article

    Muona et al Chembiochem. 2008 Dec 15. 9(18):2958-61.

    Insert

    Ulp1 (ULP1 Budding Yeast)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Ulp1(403-621)

    Available Since

    Sept. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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