29,729 results

1. pCas-Cas12m

   Plasmid

   #192274

   Purpose

   Encodes MmCas12m under a constitutive promoter

   Depositor

   John van der Oost

   Article

   Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

   Insert

   MmCas12
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PJ23108

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. RGB-S reporter

   Plasmid

   #207841

   Purpose

   A three-colour stress biosensor for real time analysis of physiological stress, genotoxicity, and cytotoxicity of Escherichia coli

   Depositor

   Christof Niemeyer

   Article

   Zoheir et al NPJ Biofilms Microbiomes. 2023 Aug 21;9(

   Inserts

   RpoH sensing construct
   SOS sensing construct
   RpoS sensing construct
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PosmY, PsulA, and grpE

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLG003 (Druantia type I)

   Plasmid

   #157881

   Purpose

   Expresses the Druantia type I defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   Druantia type I
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMMB67EH-CFP

   Plasmid

   #90103

   Purpose

   CFP control expression plasmid

   Depositor

   Samuel Miller

   Article

   Christen et al Science. 2010 Jun 4;328(5983):1295-7. do

   Insert

   CFP
   

   Use

   Broad-host range

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptac

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSHS212

   Plasmid

   #87346

   Purpose

   Cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid

   Depositor

   Jennifer Doudna

   Article

   Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

   Insert

   Cas9
   

   Use

   Tags

   His-MBP

   Expression

   Bacterial

   Mutation

   C80S/C574S

   Promoter

   T7

   Available since

   March 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. Pet28a_Synthetic_Human_H2AZ.1

   Plasmid

   #42629

   Depositor

   Joe Landry

   Insert

   Human H2AZ.1 (H2AZ1 Human, Codon Optimized, Synthetic)
   

   Use

   Tags

   Untagged

   Expression

   Bacterial

   Mutation

   Codon Optimized

   Promoter

   T7

   Available since

   March 21, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pSUB11

   Plasmid

   #169699

   Purpose

   Template plasmid for generating C-terminal 3xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.

   Depositor

   Nara Figueroa

   Article

   Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98

   Insert

   3xFLAG tag adjacent to FRT-flanked aph (KanR) gene
   

   Use

   Tags

   3xFLAG tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCHERRY3

   Plasmid

   #24659

   Depositor

   Tanya Parish

   Article

   Carroll et al PLoS One. 2010 . 5(3):e9823.

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 9, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. p11d-tRPA (MSW#111)

   Plasmid

   #208066

   Purpose

   expression of human Replication Protein A in E. coli

   Depositor

   Marc Wold

   Article

   Henricksen et al J Biol Chem. 1994 Apr 15;269(15):11121-3

   Insert

   RPA1, RPA2, RPA3 (RPA1, RPA3, RPA2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   RPA32 has a point mutation Thr88(ACC) -> Ile(A…

   Promoter

   Available since

   Nov. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAtetO_6xHis-Bst-LF

    Plasmid

    #145799

    Purpose

    Plasmid for the aTc-inducible expression of the large-fragment of Bst DNAP

    Depositor

    Andrew Ellington

    Article

    Bhadra et al mSphere. 2021 May 19;6(3):e00911-20. doi

    Insert

    Bst-LF
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    tet PA

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pYTK-DN3

    Plasmid

    #180284

    Purpose

    Spacer cassette (TU2)

    Depositor

    Andreas Milias-Argeitis

    Article

    Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

    Insert

    ConL1 - Spacer - ConR2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET42b-AncBE4max

    Plasmid

    #165157

    Purpose

    Expresses NLS-AncBE4max-NLS with an N-terminal His Tag (6xHis) for bacterial expression

    Depositor

    Taekjip Ha

    Article

    Zou et al Mol Cell. 2021 Apr 1;81(7):1553-1565.e8.

    Insert

    AncBE4max
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDW363

    Plasmid

    #8842

    Depositor

    David Waugh

    Article

    Tsao et al Gene. 1996 Feb 22. 169(1):59-64.

    Type

    Empty backbone

    Use

    Tags

    MBP and biotin acceptor peptide

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-15b_LC3B

    Plasmid

    #73949

    Purpose

    For recombinant expression of human LC3B/MAP1LC3B in E. coli

    Depositor

    Dieter Willbold

    Article

    Ma et al J Biol Chem. 2013 Dec 27;288(52):37204-1

    Insert

    LC3B (MAP1LC3B Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pHflu4

    Plasmid

    #203882

    Purpose

    pHI1 with insertion of monomeric red fluorescent protein (mRFP)

    Depositor

    Bogumil Karas

    Insert

    monomeric red fluorescent protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDusk

    Plasmid

    #43795

    Purpose

    Empty backbone for blue-light-regulated expression of a gene of interest. Expression is repressed under blue illumination.

    Depositor

    Andreas Moeglich

    Article

    Ohlendorf et al J Mol Biol. 2012 Mar 2;416(4):534-42. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDx_mScarlet3-NCwt-SYFP2

    Plasmid

    #189766

    Purpose

    Dual expression vector for bacteria and mammalian cells producing mScarlet3-NCwt-SYFP2 fusion protein for FRET analysis

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet3-NCwt-SYFP2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial and Mammalian

    Mutation

    encodes the expected internal mutations relative …

    Promoter

    CMV & Rha

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAL-hCul1-NTD

    Plasmid

    #29518

    Depositor

    Ning Zheng

    Insert

    CUL1 (CUL1 Human)
    

    Use

    Tags

    Thrombin Cleavage Site

    Expression

    Bacterial

    Mutation

    N-terminal 415 aa

    Promoter

    Available since

    May 26, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pET31b-T7-Spinach2

    Plasmid

    #79783

    Purpose

    Expresses Spinach2 under a T7 promoter

    Depositor

    Ming Hammond

    Article

    Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

    Insert

    T7-tRNA-Spinach2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDiGi

    Plasmid

    #59323

    Purpose

    Inducible expression of dsRed and GFP in bacteria

    Depositor

    Sophie Helaine, David Holden

    Article

    Helaine et al Proc Natl Acad Sci U S A. 2010 Feb 23;10

    Inserts

    EGFP
    dsRed
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pbad and Plac

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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