30,424 results

1. pFC334

   Plasmid

   #87846

   Purpose

   AMA1 plasmid with Aspergillus optimized Cas9, argB selection marker and yA specific sgRNA expressed with ribozymes

   Depositor

   Uffe Mortensen

   Article

   Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

   Inserts

   Cas9
   argB
   yA specific sgRNA
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   A. nidulans gpdA promoter with Hammerhead ribozym…

   Available Since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-28c(+)-YTHDF3

   Plasmid

   #102276

   Purpose

   For bacterial expression of His-tagged human YTHDF3

   Depositor

   Samie Jaffrey

   Article

   Patil et al Nature. 2016 Sep 7;537(7620):369-373. do

   Insert

   YTHDF3 (YTHDF3 Human)
   

   Use

   Tags

   6X HIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. DiLiCre 2.0

   Plasmid

   #198752

   Purpose

   A Cre recombinase that is expressed upon doxycycline when combined with the UbC-blasticidine-P2A-TETon-3G transactivator. DiLiCre 2.0 is a cre recombinase that is activated by violet light.

   Depositor

   Jacco van Rheenen

   Article

   Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:

   Insert

   Cre-PhoCI-ERT2-ERT2
   

   Use

   Lentiviral

   Tags

   Cre, kept in a photocleavable site, followed by 2…

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   TRE3GV

   Available Since

   July 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. MAC3-tag-N

   Plasmid

   #185482

   Purpose

   Gateway compatible destination vectors containing StrepIII/HA/UltralD

   Depositor

   Markku Varjosalo

   Article

   Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

   Type

   Empty backbone

   Use

   Tags

   StrepIII-HA-UltraID

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET19-TdTwt

   Plasmid

   #207466

   Purpose

   Bacterial expression of Terminal deoxynucleotidyl transferase (TdT)

   Depositor

   Jay Keasling

   Article

   Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

   Insert

   TdTwt (Dntt Mouse)
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET21a-ValRS-His

   Plasmid

   #124122

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Valyl-tRNA ligase (valS Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-His6-ERK2-MEK1_R4F_coexpression

   Plasmid

   #39212

   Depositor

   Melanie Cobb

   Article

   Khokhlatchev et al J Biol Chem. 1997 Apr 25;272(17):11057-6

   Inserts

   Mek1 R4F (MAP2K1 Human)
   Erk2 (Mapk1 Rat)
   

   Use

   Tags

   His6 and RBS

   Expression

   Bacterial

   Mutation

   MEK1(R4F) = S218E, S222D and deletion of AA32-51

   Promoter

   T7 and T7 via RBS

   Available Since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. 6-His-MBP-TEV-FnCpf1

   Plasmid

   #90094

   Purpose

   Bacterial expression plasmid for protein purification

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   FnCpf1 (humanized)
   

   Use

   Tags

   3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT7C-BAG2

   Plasmid

   #177654

   Purpose

   Recombinant protein synthesis of human BAG2

   Depositor

   Jeff Kuret

   Article

   Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

   Insert

   BAG2 (BAG2 Human)
   

   Use

   Tags

   6xHis-Thrombin

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMVV102

    Plasmid

    #215308

    Purpose

    Expresses sfGFP under induction with the IPTG Marionette Cassette (LacIAM + PTac-sfGFP) with a BBR1 backbone.

    Depositor

    Brian Pfleger

    Article

    Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    PTac

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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