32,389 results
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Plasmid#150814PurposeExpresses proteins in the periplasm as fusions to a high-binding mutant (A313V) maltose-binding proteinDepositorTypeEmpty backboneTagsmalE A313VExpressionBacterialPromotertacAvailable SinceJuly 22, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits
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pDIV151
Plasmid#226180PurposeExpresses Cas9 in a backbone compatible with K. phaffii.DepositorInsertCas9
UseCRISPRTagsSV40ExpressionBacterial and YeastPromoterGAP1Available SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJMP1189
Plasmid#119257Purposerfp "test" strainDepositorInsertdcas9
ExpressionBacterialMutationD10A and H840AAvailable SinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1-Strep-II
Plasmid#224209PurposeMembrane scaffold protein (MSP1D1) with Strep-II tag. pMSP1D1, 7xHis tag swapped for a Strep-II tag.DepositorInsertMSP1D1-Strep-II
TagsStrep-II TagExpressionBacterialMutationSubstitution of 7xHis tag found in pMSP1D1 for a …PromoterT7Available SinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYZ205
Plasmid#104586PurposeExpress SNAP-6His-CBDDepositorInsertSNAP-6His-CBD
ExpressionBacterialPromoterT7Available SinceJan. 4, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
TXNRD1
Plasmid#38863PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pYC2085
Plasmid#158721PurposeCRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using hygromycin as a selection marker.DepositorInsertSth1 sgRNA scaffold
UseCRISPRExpressionBacterialAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-CUL5-pCMV7.1
Plasmid#155024PurposeN-terminal 3xFLAG-tagged CUL5 in a pCMV7.1 vectorDepositorAvailable SinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pComb3HSS
Plasmid#64133Purpose2nd generation plasmid for phage display on modified geneIII, contains stuffer fragmentDepositorInsertsSS Stuffer
Light Chain Stuffer
Heavy Chain Stuffer
UsePhage displayTagsgeneIIIExpressionBacterialPromoterlacZAvailable SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
SpyTag-sfGFP
Plasmid#184226PurposeExpresses SpyTag-superfolder GFP in the bacterial cytoplasm. SpyTag enables covalent reaction with SpyCatcher.DepositorInsertSpyTag-sfGFP
TagsHis6, SpyTag, and TEV protease cleavage siteExpressionBacterialPromoterT5Available SinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pBad-CA TAG93
Plasmid#105667Purposeexpresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 93 and C-terminal 6x his tagDepositorArticleInserthuman carbonic anhydrase II thermostable variant (CA2 Human)
Tags6x his tagExpressionBacterialMutationTAG stop codon at site 93PromoterpBad/arabinoseAvailable SinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pS2513·PHP
Plasmid#122590PurposeConstitutive expressed pHiorine2 for pH quan. in gram-negative cellsDepositorInsertphIorine2
UseSynthetic BiologyExpressionBacterialPromoterPem7Available SinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-3xFLAG-HSP27
Plasmid#129229PurposeEncodes FLAG tagged human HSP27 for purification by GST affinityDepositorAvailable SinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
All_in_one_CRISPR/Cas9_LacZ
Plasmid#74293Purpose"All-in-one" CRISPR/Cas9 (wt) plasmid for cloning of custom gRNA with blue/white screeningDepositorInsertsLacZ-alpha
Cas9
mCherry
UseCRISPRTagsHAExpressionBacterial and MammalianPromoterLac promoter, SV40, and T7 promoterAvailable SinceMay 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
PET-B-HeFSpCas9-NLS-6xHis
Plasmid#207392PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertB-HeFSpCas9
UseCRISPRTags6xHisExpressionBacterialMutationN497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A…PromoterT7Available SinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNO286-3
Plasmid#107746PurposeKey genes: "mini-CcaS #10", ho1, pcyA, spectinomycin resistance, and medium copy backbone (p15a)DepositorInsertsCcaS
Ho1
PcyA
UseSynthetic BiologyExpressionBacterialMutationDeleted AA 223 and 512PromoterJ23106 and J23108Available SinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpyTag002-MBP
Plasmid#102831PurposeBacterial expression of SpyTag002 fused to maltose binding proteinDepositorInsertSpyTag002-MBP
TagsHis6 and Thrombin cleavage siteExpressionBacterialMutationPeptide tag added to the N-terminus of MBP via a …PromoterT7Available SinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pExp-DsbC
Plasmid#112564PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbC (leaderless thiol:disulfide interchange protein DsbC from E. coli) fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneTags8xHis-DsbC and Strep tagExpressionBacterialAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMApra-Para
Plasmid#218362PurposeEmpty backbone with araC-ParaBAD expression systemDepositorTypeEmpty backboneExpressionBacterialAvailable SinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only