30,749 results

1. L4440_BioBrick-hif-1-sgRNA-B

   Plasmid

   #177785

   Purpose

   Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   hif-1 (hif-1 Nematode)
   

   Expression

   Bacterial

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWT PolI

   Plasmid

   #11721

   Depositor

   Lawrence Loeb

   Article

   Camps et al Proc Natl Acad Sci U S A. 2003 Aug 19. 1

   Insert

   DNA polymerase I (polA E. coli)
   

   Expression

   Bacterial

   Mutation

   wild-type

   Available Since

   Aug. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. PET-28a-YTH domain of YTHDF2

   Plasmid

   #228776

   Purpose

   Protein expression plasmid for YTH domain of YTHDF2 in E.Coli.

   Depositor

   Huiqing Zhou

   Article

   Wang et al RSC Chem Biol. 2024 Jul 25;5(9):914-923.

   Insert

   YTH domain of YTHDF2 (YTHDF2 Human)
   

   Tags

   6*His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. CREBBP

   Plasmid

   #38977

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   CREBBP (CREBBP Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pBADZ-HisCre

   Plasmid

   #111187

   Purpose

   Helper plasmid containing Cre-recombinase gene, which is expressed in DH10MultiBac cells

   Depositor

   Tim Richmond

   Article

   Berger et al Nat Biotechnol. 2004 Dec;22(12):1583-7.

   Insert

   Cre recombinase
   

   Tags

   His tag

   Expression

   Bacterial

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSETb-pcDronpa2

   Plasmid

   #78184

   Purpose

   Expresses pcDronpa2 in E. coli strains

   Depositor

   Peter Dedecker

   Article

   Moeyaert et al ACS Nano. 2014 Feb 25;8(2):1664-73. doi:

   Insert

   pcDronpa2
   

   Tags

   6xHis-tag; Xpress tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSKDuet01

   Plasmid

   #12172

   Depositor

   Hideo Iwai

   Article

   Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

   Insert

   NpuDnaE
   

   Tags

   His-GB1

   Expression

   Bacterial

   Available Since

   Dec. 14, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. p404TDH3

   Plasmid

   #15973

   Depositor

   Nicolas Buchler, Fred Cross

   Insert

   TDH3 promoter + polylinker + CYC1 terminator (TDH3 Budding Yeast)
   

   Expression

   Bacterial and Yeast

   Mutation

   TDH3 promoter is between SacI and XbaI restrictio…

   Available Since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKM493

   Plasmid

   #140191

   Purpose

   ORBIT integrating plasmid for C-terminal tagging with cleavable EGFP.

   Depositor

   Kenan Murphy

   Article

   Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

   Insert

   TEV-Flag-Gly4-eGFP
   

   Tags

   Flag + eGFP

   Expression

   Bacterial

   Promoter

   PGroEL

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYUBDuet

    Plasmid

    #37278

    Depositor

    Ted Baker

    Article

    Bashiri et al PLoS One. 2010 Dec 29;5(12):e15803.

    Type

    Empty backbone

    Use

    Mycobacteria shuttle vector

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDB179-Z1-PETase

    Plasmid

    #218703

    Purpose

    Encodes codon optimized Z1-PETase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    Z1-PETase
    

    Tags

    10xHis

    Expression

    Bacterial

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBAD/HisB-iRFP702

    Plasmid

    #45455

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Insert

    iRFP702
    

    Expression

    Bacterial

    Mutation

    T2A, S45T, D124E, M170L, D202L, I203V, I219V, R23…

    Promoter

    araBAD

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHPSE-P6

    Plasmid

    #185907

    Purpose

    Engineered Human Heparanase mutant for e.coli expression

    Depositor

    Colin Jackson

    Article

    Whitefield et al RSC Chem Biol. 2022 Feb 15;3(3):341-349.

    Inserts

    HPSE1 (HPSE Human)
    HPSE1 (HPSE Human, Synthetic)
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBAD_TypeIC_Cascade_cas3_invivo

    Plasmid

    #196400

    Purpose

    expresses CRISPR Cascade-Cas3 Type IC proteins for in vivo assay

    Depositor

    David Taylor

    Article

    O'Brien et al Mol Cell. 2023 Mar 2;83(5):746-758.e5. d

    Inserts

    Cas5c
    Cas7c
    Cas8c
    Cas3c
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. His-Tev-UbE2D3

    Plasmid

    #186805

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses ubiquitin conjugating enzyme His-Tev-UbE2D3.

    Depositor

    Lalit Deshmukh

    Article

    Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

    Insert

    His-Tev-UbE2D3 (UBE2D3 Human)
    

    Tags

    His6-Tev

    Expression

    Bacterial

    Available Since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBMM42

    Plasmid

    #13331

    Depositor

    Martin Gellert

    Article

    Modesti et al EMBO J. 1999 Apr 1. 18(7):2008-18.

    Insert

    XRCC4 (XRCC4 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pLEVI(408)-ColE-iLight-msfGFP

    Plasmid

    #170268

    Purpose

    Expresses LexA_DBD-iLight-msfGFP under J23116 promoter and mCherry under ColE promoter with a LexA408 operator in bacteria.

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

    Insert

    iLight
    

    Tags

    msfGFP

    Expression

    Bacterial

    Mutation

    To design iLight for expression in bacteria, a fu…

    Promoter

    pJ23116

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET15TV-L-hlPYK WT

    Plasmid

    #187975

    Purpose

    Expresses the human liver pyruvate kinase

    Depositor

    Audrey Lamb

    Article

    McFarlane et al Acta Crystallogr F Struct Biol Commun. 2

    Insert

    hlPYK WT
    

    Tags

    Hexahistag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLPT234

    Plasmid

    #127855

    Purpose

    Repressilator 2.0, without degradation tags, integrated triple reporter

    Depositor

    Johan Paulsson

    Article

    Riglar et al Nat Commun. 2019 Oct 11;10(1):4665. doi:

    Inserts

    bla
    mSCFP3
    mKate2
    mVenus
    TetR
    LacI
    cI
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    First 11aa derived from mCherry as fusion protein

    Promoter

    pAmpR, pL LacO1, pL tetO1, and pR

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZA31-sulA-GFP

    Plasmid

    #78493

    Purpose

    Expression of GFP under the sulA promoter.

    Depositor

    David Bikard

    Article

    Cui et al Nucleic Acids Res. 2016 Apr 8. pii: gkw2

    Insert

    GFPmut2
    

    Expression

    Bacterial

    Available Since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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