31,059 results
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Plasmid#195487PurposeBacterial expression plasmid with HaloTag v7 insert; modified to replace cysteines C61, C262, and optimized to human codon bias/preference for use in oxidizing environments.DepositorInsertHaloTag.v7
Tags6xHISExpressionBacterialMutationC61T, C262VPromoterT7Available SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMDH
Plasmid#133129PurposeA recombinant plasmid expressing mdh (malate dehydrogenase) geneDepositorInsertmalate dehydrogenase gene
ExpressionBacterialPromotermalate dehydrogenase promoterAvailable SinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNBU2_repA3
Plasmid#211912PurposeReplicative vector for protein expression in Bacteroidales with an aTc inducible promoter, terminator, and a barcoding site.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceFeb. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
KDM4D
Plasmid#73247PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertKDM4D (KDM4D Human)
TagsHis-6-TEVExpressionBacterialMutationpfam02375:JmjN 18-64, pfam02373:JmjC 182-295PromoterT7Available SinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSB233
Plasmid#164128PurposePP7-CFP and PhlF-RFP expression plasmidDepositorInsertPP7-CFP
ExpressionBacterialPromoterPtetAvailable SinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFLAG_OriM
Plasmid#110097PurposeAnalogous to pFLAG_attP, but containing oriM instead of attP, thus being a replicative vector.DepositorTypeEmpty backboneTags3xFLAGExpressionBacterialAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
Pediculus humanus corporis PINK1 (115 - 575)
Plasmid#110750PurposeBacterial expression of PhPINK1 (biochemistry)DepositorInsertPhum_PHUM577390 (Phum_PHUM577390 Pediculus humanus corporis)
TagsN-His6-GST-3CExpressionBacterialPromoterT7Available SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher
Plasmid#216284PurposeExpresses SpyCatcher003-SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow stepwise coupling of two unique SpyTag-linked bindersDepositorInsertDoubleCatcher
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFLAG_attP
Plasmid#110095PurposeVector for low-level constitutive expression in mycobacteria from the Tet promoter. Lacks the Tet repressor. Contains attP for chromosomal integration. Lacks the integrase and thus remains stably integrated in the absence of antibiotic selection. Contains a C-terminal 3xFLAG to allow detection of expression levels by Western blotting.DepositorTypeEmpty backboneTags3xFLAGExpressionBacterialAvailable SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD2 (full-length, aa 1-348)
Plasmid#61409PurposeExpresses human OTUD2 (full-length) in E. coli.DepositorAvailable SinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MH6-RspCas13d_RspCasWYL1
Plasmid#108304PurposeExpresses E. coli codon optimized RspCas13d and RspCasWYL1 in the pET28a backbone.DepositorInsertsRspCas13d
RspWYL1
UseCRISPRTagsmH6 tagExpressionBacterialPromoterlacAvailable SinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET151-GFP-PP-RPN2 (940-953)
Plasmid#73746PurposeExpression of GFP-PP-RPN2 (940-953) fusion in E.coliDepositorInsertRPN2 (RPN2 Human)
Tags6x His and GFPExpressionBacterialMutationConstruct possesses only residues 940-953 of RPN2PromoterT7Available SinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-Pin1
Plasmid#19027DepositorAvailable SinceAug. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
pVoPo-03
Plasmid#187838PurposeBackbone for inducible expression system in Fusobacterium nucleatumDepositorTypeEmpty backboneExpressionBacterialAvailable SinceSept. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSA10(gfcD)
Plasmid#176191PurposegfcD protein expressionDepositorInsertgfcD
Tags6xHisExpressionBacterialPromoterPtacAvailable SinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAT112TAG-SepT
Plasmid#34624DepositorInserttRNA^Sep
ExpressionBacterialPromoterlppAvailable SinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GNAI2_G-alpha
Plasmid#109791PurposeProtein expression and purification of GNAI2_G-alphaDepositorInsertGNAI2_G-alpha (GNAI2 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
MP28444 – pFUSE2ss-aRD114_CHIg-mIgG2A
Plasmid#110555PurposepFUSE backbone with an IL2 signal sequence, expressing the variable heavy chain of anti-RD114 binder fused to murine IgG2A constant heavy chain.DepositorInsertAnti-RD114 heavy chain
TagsmIgG2AExpressionBacterial and MammalianPromoterEF-1 alpha core promoterAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-162
Plasmid#118543PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertsGFP2
UseMinitn5 delivery vectorExpressionBacterialAvailable SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJMP8923
Plasmid#220904PurposeMobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (BsaI site), and PG-dCas9-novoDepositorInsertdCas9-novo
UseCRISPRTags3xMycExpressionBacterialMutationD10A, H840AAvailable SinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only