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pBad-HisB-RRvT

Plasmid

#87364

Purpose

Expresses RRvT, a tandem heterodimer red fluorescent protein

Depositor

Robert Campbell

Article

Wiens et al Chembiochem. 2016 Oct 26. doi: 10.1002/c

Insert

RRvT

Use

Tags

His-Tag

Expression

Bacterial

Mutation

Promoter

pBad (Arabinose)

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pET-21a(+)/MBP-His-PINK1

Plasmid

#38005

Depositor

Michael J Fox Foundation MJFF

Insert

PINK1 (PINK1 Human)

Use

Tags

6xHIS and MBP

Expression

Bacterial

Mutation

Promoter

Available since

March 10, 2014

Availability

Academic Institutions and Nonprofits only
pMRE147

Plasmid

#118499

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mClover3

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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p1A

Plasmid

#162692

Purpose

Expresses H. neapolitanus CbbLS and S. elongatus Prk. Rescues CCMB1 in M9 glycerol at 10% CO2

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

H. neapolitanus CbbLS and S. elongatus Prk

Use

Tags

N terminal 6x His tag on PRK

Expression

Bacterial

Mutation

Promoter

PLtet0-1 promoter

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pDae029 (eMutaT7[TadA-8e])

Plasmid

#187620

Purpose

Expresses eMutaT7[TadA-8e] for mutagenizing genes downstream from a T7 promoter in e.coli

Depositor

Seokhee Kim

Article

Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

Insert

TadA-8e-T7RNAP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pGEx-4T-1-YTHDF2

Plasmid

#52301

Purpose

Encode YTHDF2 isoform 2 with N-terminal GST tag for E. coli expression

Depositor

Chuan He

Article

Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

Insert

YTHDF2 (YTHDF2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
PTP1B human

Plasmid

#102719

Purpose

human PTP1B catalytic domain (residues 1-301) in RP1B vector

Depositor

Wolfgang Peti

Article

Choy et al Mol Cell. 2017 Feb 16;65(4):644-658.e5.

Insert

Protein Tyrosine Phosphatase 1B (PTP1B) (PTPN1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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AdML

Plasmid

#11242

Depositor

Robin Reed

Article

Luo et al Proc Natl Acad Sci U S A. 1999 Dec 21. 9

Insert

AdML

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only
pET-30 Ubwt (2)

Plasmid

#26685

Depositor

Linda Hicke

Article

French et al J Biol Chem. 2009 May 1. 284(18):12071-9

Insert

Ubiquitin

Use

Tags

His and S-tag

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 17, 2010

Availability

Academic Institutions and Nonprofits only
HSA2_His

Plasmid

#202764

Purpose

human serum albumin design HSA2, in pET29b with C-terminal 6His-tag

Depositor

Sarel Fleishman

Article

Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

Insert

HSA2

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pTPH405c

Plasmid

#185726

Purpose

Directed evolution of Cas9 adenine base editors with altered PAM compatibility

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

gIII(N)-NpuN-NpuC-gIII(C) single PAM linker

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUD2 (full-length, aa 1-348)

Plasmid

#61409

Purpose

Expresses human OTUD2 (full-length) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD2 (YOD1 Human)

Use

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pPGC-K

Plasmid

#174581

Purpose

pOPIN-GG expression vector compatible with C-terminal tagging, Kanamycin resistant

Depositor

Mark Banfield

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pWZ854

Plasmid

#208116

Purpose

mSwAP-In marker cassette 2 with 5’PB-mNeongreen-HPRT1-Neo

Depositor

Jef Boeke

Article

Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

Insert

mNeonGreen-HPRT1-Neo

Use

Tags

T2A

Expression

Bacterial and Mammalian

Mutation

Promoter

EF1alpha

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pWZ852

Plasmid

#208114

Purpose

mSwAP-In marker cassette 1 with mScarlet-HygTK

Depositor

Jef Boeke

Article

Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

Insert

mScarlet-HygTK

Use

Tags

T2A

Expression

Bacterial and Mammalian

Mutation

Promoter

EF1alpha

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-Cys-ELP(40nm)-LPETGG

Plasmid

#90475

Purpose

Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGG

Depositor

Hermann Gaub

Article

Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

Insert

Cys-ELP(40nm)-LPETGG

Use

Tags

Cysteine and Sortase-Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD_M337V

Plasmid

#98674

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with M337V

Depositor

Nicolas Fawzi

Article

Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

Insert

TDP-43 (TARDBP Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

M337V

Promoter

Available since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pPath-BGC08-SGE

Plasmid

#200006

Purpose

BGC08 pathway. SGE

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

Pathways

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pBR322-Pdp1-HiBiT

Plasmid

#198310

Purpose

Loads HiBiT-tagged Pdp1 into PVCs; for payload loading assays; also contains Pnf

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1-HiBiT

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pTO149_RFP_CauNEO

Plasmid

#177277

Purpose

Encodes a G418 (NEOr) selectable marker with codon-optimization for expression in CUG clade fungi. Also encodes RFP tagging of Candida auris ENO1 to measure transformation efficiency.

Depositor

Teresa O'Meara

Article

Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:

Insert

RFP, CauNEO

Use

Tags

ENO1-RFP

Expression

Bacterial

Mutation

All CUG Leucine codons were replaced with the syn…

Promoter

AgTEF2

Available since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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