30,753 results

1. pTn7xTS_SnoopCatcher:mNG

   Plasmid

   #225191

   Purpose

   Used for inserting SnoopCatcher fused to mNeonGreen at the bacterial Tn7 site

   Depositor

   Travis Wiles

   Article

   Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

   Insert

   SnoopCatcher:mNeonGreen
   

   Tags

   SnoopCatcher

   Expression

   Bacterial

   Available Since

   April 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSL0284 (pQCascade_entry)

   Plasmid

   #130635

   Purpose

   Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array contains two BsaI sites for spacer cloning.

   Depositor

   Samuel H. Sternberg

   Article

   Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

   Insert

   VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)
   

   Use

   CRISPR; Transposon

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAD43-daf-16 RNAi

   Plasmid

   #34833

   Depositor

   Cynthia Kenyon

   Article

   Dillin et al Science. 2002 Oct 25;298(5594):830-4.

   Insert

   daf-16 (daf-16 Nematode)
   

   Use

   RNAi

   Expression

   Bacterial

   Available Since

   June 13, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pVC-Ds-E1b:eGFP-Ds

   Plasmid

   #102417

   Purpose

   Fluorescent vector for screening enhancer elements in zebrafish

   Depositor

   Tatjana Sauka-Spengler

   Article

   Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

   Insert

   E1b:eGFP
   

   Expression

   Bacterial

   Promoter

   Zebrafish E1b minimal promoter

   Available Since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX-4T-1 3xFlag-MAPKAPK2

   Plasmid

   #82720

   Purpose

   Expresses human MAPKAPK2

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   MAPKAPK2 (MAPKAPK2 Human)
   

   Tags

   3xFLAG and GST

   Expression

   Bacterial

   Available Since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

6. pDuBir-Lbu-dCas13a-avitag

   Plasmid

   #100817

   Purpose

   Dual expression of Lbu-dCas13a with a C-terminal avi-tag and BirA to biotinylate Lbu-dCas13a

   Depositor

   Mitchell O'Connell

   Article

   Tambe et al Cell Rep. 2018 Jul 24;24(4):1025-1036. d

   Insert

   Lbu_Cas13a_R472A_H477A_R1048A_H1053A
   

   Tags

   AviTag and His6-MBP

   Expression

   Bacterial

   Mutation

   R472A, H477A, R1048A, H1053A

   Promoter

   T7

   Available Since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNCS-CyOFP

   Plasmid

   #74278

   Purpose

   Constitutive expression of CyOFP in bacteria

   Depositor

   Michael Lin

   Article

   Chu et al Nat Biotechnol. 2016 May 30. doi: 10.103

   Insert

   CyOFP
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEND-321_Cacnes_ori

   Plasmid

   #225603

   Purpose

   Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a high-copy pBR322 E. coli ori

   Depositor

   Marc Güell

   Article

   Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEND-350_Cacnes_ori

   Plasmid

   #225605

   Purpose

   Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)

   Depositor

   Marc Güell

   Article

   Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEND-446_Cacnes_ProA

    Plasmid

    #225609

    Purpose

    Suicide C. acnes vector to generate ProA (PPA_RS04250) deletion leaving an Ery resistance cassette flanked by BxbI recombination sites

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEND-224_Cacnes_sfGFP

    Plasmid

    #225611

    Purpose

    Replicative vector for green fluorescent reporter protein sfGFP strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+sfGFP.

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Codon optimized for Cutibacterium acnes

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTlpA_mCherry_Txe/Axe

    Plasmid

    #229147

    Purpose

    Toxin-Antitoxin (Txe/Axe)

    Depositor

    Shrikrishnan Sankaran

    Article

    Dey et al Microb Biotechnol. 2023 Jun;16(6):1264-1

    Insert

    mCherry, Txe/Axe
    

    Expression

    Bacterial

    Promoter

    PtlpA (mCherry), Txe/Axe (Native)

    Available Since

    Jan. 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET21-pelB-GST-2

    Plasmid

    #228984

    Purpose

    For bacterial expression of anti-GST nanobody GST-2, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    GST-2
    

    Tags

    6xHIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET21-pelB-LaG94-1--LaG94-14

    Plasmid

    #228973

    Purpose

    For bacterial expression of anti-GFP nanobody dimer of LaG94-1 and LaG94-14, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaG94-1--LaG94-14
    

    Tags

    6xHIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET21-pelB-GST-1

    Plasmid

    #228983

    Purpose

    For bacterial expression of anti-GST nanobody GST-1, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    GST-1
    

    Tags

    6xHIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET21-pelB-LaTdT-1

    Plasmid

    #228974

    Purpose

    For bacterial expression of anti-tdTomato nanobody LaTdT-1, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaTdT-1
    

    Tags

    6xHIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET21-pelB-LaRIgG-7

    Plasmid

    #228997

    Purpose

    For bacterial expression of anti-rabbit IgG nanobody LaRIgG-7, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaRIgG-7
    

    Tags

    6xHIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21-pelB-LaRIgG-1

    Plasmid

    #228995

    Purpose

    For bacterial expression of anti-rabbit IgG nanobody LaRIgG-1, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaRIgG-1
    

    Tags

    6xHIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET21-pelB-LaMIgG-5

    Plasmid

    #228991

    Purpose

    For bacterial expression of anti-mouse IgG nanobody LaMIgG-5, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaMIgG-5
    

    Tags

    6xHIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pETduet+sfGFP-TetR

    Plasmid

    #157794

    Purpose

    Expression of sfGFP-TetR fusion protein

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    sfGFP-TetR
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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