29,747 results
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Plasmid#32056PurposeVector optimized for constitutive gene expression in Burkholderia genus and related bacteriaDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterPs7 promoter, originated from Paraburkholderia xe…Available sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only
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PZac2.1 hSyn1-Lck-13xLinker-BioID2-BioID2-HA
Plasmid#176854PurposeExpresses membrane bound biotin ligase, BioID2, in neuronsDepositorInsertLck-13xLinker-BioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialMutationPromoterhSyn1Available sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNM262
Plasmid#128412PurposeVanillic acid inducible production of lysostaphin. LsyT with the amyE signaling peptide for secretion.DepositorInsertLysostaphin (epr Staphylococcus simulans)
UseSynthetic BiologyTagsamyE SPExpressionBacterialMutationPromoterPspank(V)Available sinceSept. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKD239-1g-2
Plasmid#90959PurposeExpresses S. baltica TtrR and mCherry constitutively and sfGFP under the PttrB185-269 promoterDepositorInsertsTetrathionate response regulator
Superfolder GFP
mCherry
UseTagsExpressionBacterialMutationPromoterBba_J23100, Bba_J23109, and PttrB185-269Available sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFP.R268
Plasmid#138180PurposeExpresses the split mCherry reporter interrupted by the gp41-8 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-mCherry
Plasmid#109421PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCherry. VHH-mCherry also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, mCherry, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, and T7ExpressionBacterialMutationPromoterT7Available sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD-PSmOrange
Plasmid#31886DepositorInsertPSmOrange
UseTagsExpressionBacterialMutationS21T, Q63L, F100Y, L125M, K166R, P192S (mutations…PromoterAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pExodus CMV.Trex2
Plasmid#40210PurposeMammalian expression of codon-optimized Trex2.DepositorInsertThree prime repair exonuclease 2 (Trex2 Mouse)
UseTagsExpressionBacterial and MammalianMutationPromoterCMV and T7Available sinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
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AviTag-SpyTag003-MBP
Plasmid#184227PurposeExpresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.DepositorInsertAviTag-SpyTag003-MBP
UseTagsAviTag, His6, SpyTag003, and Thrombin cleavage si…ExpressionBacterialMutationPromoterT7Available sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMVV099
Plasmid#215305PurposeExpresses sfGFP under induction with the Cuminic Acid Marionette Cassette (CymRAM + PCymRC-sfGFP) with a BBR1 backbone.DepositorInsertsfGFP
UseSynthetic BiologyTagsNoneExpressionBacterialMutationPromoterPCymRCAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD099
Plasmid#212923PurposeA MoClo Yeast Toolkit LEU2, CEN/ARS empty vector (GFP dropout). Contains ConL1, part234 GFP dropout, ConR2, LEU2, CEN6/ARS4, AmpR-ColE1.DepositorInsertGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 AMSH 1-178
Plasmid#160050PurposeExpresses MIT domain (residues 1-178) of AMSH as a HIS-SUMO fusion protein. Internal ID: WISP18-22.DepositorInsertAMSH residues 1-178 (STAMBP Human)
UseTagsHIS-SUMOExpressionBacterialMutationResidues 1-178PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOxyR_rfp
Plasmid#194155PurposeRed Fluorescent Reporter of intracellular hydrogen peroxideDepositorInserttranscriptional regulator, OxyR, cognate promoter PoxyS, fluorescent protein mCherry
UseTagsExpressionBacterialMutationPromoterPoxyR, PoxySAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOPINB-HOIL-1 helix-RBR (233–510)
Plasmid#193859PurposeExpression of human His-tagged HOIL-1 (RBCK1) helix-RBR (aa 233–510) codon optimized for E. coliDepositorInsertHOIL-1 (RBCK1 Human)
UseTags3C protease cleavage site and 6x-His tagExpressionBacterialMutationResidues 233–510, codon optimised for expression …PromoterT7Available sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
KG#901
Plasmid#110936PurposeExpresses Emerald-RAB-3 in the cholinergic motor neuron DA9 for imaging synaptic vesicles in a single neuron in a living animalDepositorInsertsmig-13 promoter
Emerald-RAB-3
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbB0c-RFP
Plasmid#35366DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCE1
Plasmid#174434PurposeCandida optimized monomeric eGFP with RIPLING linker sequence.DepositorInsertCandida optimized monomeric GFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTO_CHMP3_LAP
Plasmid#101849PurposeTet-inducible expression of human CHMP3 with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integrationDepositorInsertCharged Multivesicular Body Protein 3 (CHMP3 Human)
UseGateway cloning, flp-frt recombination, tet-induc…TagsLocalization and Affinity Purification (LAP) tagExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceDec. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTDpelB-C_sfYFPTwinStrep
Plasmid#45944Purposeexpresses P.putida codon optimized PelB-sfYFP-Twin-Strep fusion protein for periplasmic translocation via PelB signal sequenceDepositorInsertsynthetic sfYFP (codon usage adapted to P.putida KT2440)
UseTagsPelB export sequence and Twin-Strep-tagExpressionBacterialMutationPromoterlacIq/PtrcAvailable sinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only