Skip to main content
Addgene

29,747 results

Showing: 4001 - 4020 of 29747 results
  1. pMLS7

    Plasmid
    #32056
    Purpose
    Vector optimized for constitutive gene expression in Burkholderia genus and related bacteria
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Ps7 promoter, originated from Paraburkholderia xe…
    Available since
    Nov. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
  2. pNM262

    Plasmid
    #128412
    Purpose
    Vanillic acid inducible production of lysostaphin. LsyT with the amyE signaling peptide for secretion.
    Depositor
    Insert
    Lysostaphin (epr Staphylococcus simulans)
    Use
    Synthetic Biology
    Tags
    amyE SP
    Expression
    Bacterial
    Mutation
    Promoter
    Pspank(V)
    Available since
    Sept. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
  3. pKD239-1g-2

    Plasmid
    #90959
    Purpose
    Expresses S. baltica TtrR and mCherry constitutively and sfGFP under the PttrB185-269 promoter
    Depositor
    Inserts
    Tetrathionate response regulator
    Superfolder GFP
    mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Bba_J23100, Bba_J23109, and PttrB185-269
    Available since
    May 16, 2017
    Availability
    Academic Institutions and Nonprofits only
  4. pFP.R268

    Plasmid
    #138180
    Purpose
    Expresses the split mCherry reporter interrupted by the gp41-8 intein (in cis)
    Depositor
    Inserts
    araC (araC )
    mCherryN(LNR).gp41-8.(SAV)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    P(araBAD) and iPC
    Available since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
  5. pET24a-VHH-mCherry

    Plasmid
    #109421
    Purpose
    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCherry. VHH-mCherry also contains a T7, HA, BAP and His6 epitope
    Depositor
    Insert
    anti-GFP nanobody fused to a T7, mCherry, HA, BAP and His6 epitope
    Use
    Tags
    BAP, HA, His6, and T7
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 19, 2018
    Availability
    Academic Institutions and Nonprofits only
  6. AviTag-SpyTag003-MBP

    Plasmid
    #184227
    Purpose
    Expresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.
    Depositor
    Insert
    AviTag-SpyTag003-MBP
    Use
    Tags
    AviTag, His6, SpyTag003, and Thrombin cleavage si…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 29, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. pOPINB-HOIL-1 helix-RBR (233–510)

    Plasmid
    #193859
    Purpose
    Expression of human His-tagged HOIL-1 (RBCK1) helix-RBR (aa 233–510) codon optimized for E. coli
    Insert
    HOIL-1 (RBCK1 Human)
    Use
    Tags
    3C protease cleavage site and 6x-His tag
    Expression
    Bacterial
    Mutation
    Residues 233–510, codon optimised for expression …
    Promoter
    T7
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
  8. KG#901

    Plasmid
    #110936
    Purpose
    Expresses Emerald-RAB-3 in the cholinergic motor neuron DA9 for imaging synaptic vesicles in a single neuron in a living animal
    Depositor
    Inserts
    mig-13 promoter
    Emerald-RAB-3
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
  9. pTO_CHMP3_LAP

    Plasmid
    #101849
    Purpose
    Tet-inducible expression of human CHMP3 with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration
    Depositor
    Insert
    Charged Multivesicular Body Protein 3 (CHMP3 Human)
    Use
    Gateway cloning, flp-frt recombination, tet-induc…
    Tags
    Localization and Affinity Purification (LAP) tag
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
  10. pTDpelB-C_sfYFPTwinStrep

    Plasmid
    #45944
    Purpose
    expresses P.putida codon optimized PelB-sfYFP-Twin-Strep fusion protein for periplasmic translocation via PelB signal sequence
    Depositor
    Insert
    synthetic sfYFP (codon usage adapted to P.putida KT2440)
    Use
    Tags
    PelB export sequence and Twin-Strep-tag
    Expression
    Bacterial
    Mutation
    Promoter
    lacIq/Ptrc
    Available since
    Sept. 18, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 4001 - 4020 of 29747 results