33,906 results
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Plasmid#79526PurposeBacterial expression plasmid used for T7 promoter driven expression of recombinant proteins with N-terminal 8X His, thrombin cleavage site, SUMO, and TEV cleavage site.DepositorTypeEmpty backboneTags8xHis-Thrombin-SUMO-TEV and TEV cleavage siteExpressionBacterialAvailable sinceJune 16, 2016AvailabilityIndustry, Academic Institutions, and NonprofitsCited by
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pAN3944
Plasmid#74702PurposeCircuit plasmid: 0x1CDepositorInsertCircuit 0x1C
ExpressionBacterialAvailable sinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGEX6P1-human full-length LIC1
Plasmid#74597Purposeused for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tagDepositorInsertfull length human dynein light intermediate chain 1 (DYNC1LI1 Human)
TagsN-terminal GST and Strep IIExpressionBacterialPromoterT7Available sinceMay 2, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28-Mff(1-61)-PP-GST
Plasmid#73042PurposeBacterial expression of mouse Mff residues 1-61 with C-terminal GST-tag and PreScission protease cleavage siteDepositorInsertMff(1-61) (Mff Mouse)
TagsGST-tag and PreScission protease siteExpressionBacterialMutationTruncation containing amino acids 1-61PromoterT7Available sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET3aTr
Plasmid#64008PurposeSingle donor or transfer plasmid for source of translation cassette for plasmid suiteDepositorTypeEmpty backboneExpressionBacterialPromoterT7Available sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pZ001 (AacC2c1 locus)
Plasmid#70166PurposeExpresses AacC2c1 full locusDepositorInsertAacC2c1 locus
ExpressionBacterialAvailable sinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MSP1D1deltaH4/2H5
Plasmid#71715PurposeMembrane scaffold protein (MSP), helix 4/2 + helix5 deletionDepositorAvailable sinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
MSP2253
Plasmid#70704PurposeBacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #1DepositorInsertsmammalian codon-optimized KKH variant Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialMutationE782K, N968K and R1015H in SaCas9PromoterT7Available sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
MSP2283
Plasmid#70702PurposeBacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1DepositorInsertsmammalian codon-optimized Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialPromoterT7Available sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGM202T7
Plasmid#69993PurposeE. coli/Streptomyces expression vector containing the T7-promoterDepositorInsertsaphII
tsr
rep
sso
T7 promoter
dso
His-Orf
TagsN-terminal and C-terminal His tag encoding sequen…ExpressionBacterialAvailable sinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCR8/GW/TOPO PCGF1
Plasmid#69770PurposeGateway entry vector for PCGF1DepositorInsertPCGF1 (PCGF1 Human)
ExpressionBacterialAvailable sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET3a-hUb
Plasmid#61937PurposeExpression of hUb in E.coliDepositorAvailable sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ecALDH
Plasmid#67921PurposeExpresses aldehyde dehydrogenase from Escherichia coli, codon optimized for expression in E. coliDepositorInsertEscherichia coli aldehyde dehydrogenase (puuC Escherichia coli)
Tags6x-HisExpressionBacterialPromoterT7Available sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBSM
Plasmid#67505PurposeExpress the tRNAMet scaffoldDepositorInsertmodified tRNAMet
ExpressionBacterialPromoterlppAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBSMrnaSeph
Plasmid#67507PurposeExpress the tRNAMet scaffold with the sephadex aptamer tagDepositorInsertmodified tRNAMet with sephadex aptamer
ExpressionBacterialPromoterlppAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLJSRSF3
Plasmid#64692PurposeHis-GST-Smt3-NpuDnaEN102-CBDDepositorTypeEmpty backboneTagsCBD, GST, HisTag, NpuDnaE-N102, and Smt3ExpressionBacterialPromoterT7Available sinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Salmonella MTAN
Plasmid#64041PurposeExpression of S. enterica MTAN in E. coliDepositorInsertMethylthioadenosine nucleosidase
TagsHistidine tagExpressionBacterialAvailable sinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Fast-GCaMP6f-RS09
Plasmid#67160Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activityDepositorInsertFast-GCaMP6f-RS09
Tags6xHis, T7 tag, and Xpress tagExpressionBacterialMutationL414T within GCaMP6fPromoterT7Available sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
4R3H
Plasmid#64652PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertYTHDC1:JMC092-F09:C231531 (YTHDC1 Human)
Tags6xHisExpressionBacterialMutationSee commentsPromoterT7Available sinceApril 29, 2015AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pBEST-OR2-OR1-Pr-araC, pBAD-TetR, pBAD-TetO1-deGFP-ssrA
Plasmid#45789DepositorInsertsdeGFP
Tetracycline repressor
araC
UseSynthetic BiologyTagsssrA degradation tagExpressionBacterialPromoterOR2-OR1-Pr (bacteriophage Lambda with one mutatio…Available sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by