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pET21b Hot-PETase

Plasmid

#225761

Purpose

Expresses codon-optimized Hot-PETase in E. coli with a C-terminal his-tag

Depositor

Jason Boock

Publication

Carter et al Microb Cell Fact. 2024 Nov 25;23(1):319.

Insert

Hot-PETase

Tags

6xHis Tag

Expression

Bacterial

Promoter

T7

Available since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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elavl3:hArr-TEV;he1.1:YFP

Plasmid

#218855

Purpose

D. rerio elavl3 promoter driving expression of human Arrestin-TEV protease fusion protein for use in trans-Tango.

Depositor

Marnie Halpern

Publication

Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

Insert

hArr

Tags

human Arrestin-Tobbaco Etch Virus protease fusion…

Expression

Bacterial

Available since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pMD1 anti-GFP nanobody-Tn5

Plasmid

#215567

Purpose

Expresses anti-GFP nanobody-Tn5 fusion in bacteria with an N-terminal 3xFlag tag and a C-terminal intein-CBD-intein tag

Depositor

Isabelle Mansuy

Publication

Maria A. et al bioRxiv 2024.07.12.603224

Insert

anti-GFP nanobody-Tn5 fusion

Tags

3xFlag and intein-CBD

Expression

Bacterial

Promoter

T7

Available since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCOLA-Gent-EM7-Erv1p-DsbC

Plasmid

#202485

Purpose

Expresses sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) constitutively

Depositor

Michael Lynch

Publication

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) (ERV1 Escherichia coli, Budding Yeast)

Expression

Bacterial

Promoter

EM7

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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PEmax_2star_pet21A

Plasmid

#227675

Purpose

Expression plasmid for PEMAX** protein in bacteria.

Depositor

Scot Wolfe

Publication

Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

Insert

PEmax_2_star

Use

CRISPR

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

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HMA-APOBEC1-Nb

Plasmid

#226012

Purpose

For expression of His-MBP-APOBEC1-Nanobody protein in E.coli

Depositor

Eugene Yeo

Publication

Liang et al Nat Commun. 2024 Aug 16;15(1):7067. doi:

Insert

APOBEC1 (Apobec1 Rat)

Tags

His-MBP and antiRabbit-nanobody

Expression

Bacterial

Available since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-SUPREM

Plasmid

#205023

Purpose

Expressing SUPer Rna Ecogii Methyltransferase (SUPREM)

Depositor

Paola Laurino

Publication

Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

Insert

SUPer Rna Ecogii Methyltransferase

Tags

His6x tag

Expression

Bacterial

Promoter

T7

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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HyperNova/pRSETB

Plasmid

#221456

Purpose

Fast-maturating photosensitizing fluorescent protein for chromophore-assisted light inactivation

Depositor

Kiwamu Takemoto

Publication

Shidara et al Commun Biol. 2024 Aug 6;7(1):945. doi: 1

Insert

HyperNova

Expression

Bacterial

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBBR15.2-2H12.D11opt

Plasmid

#221185

Purpose

cdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter (Internal Lab ID: UJ11239)

Depositor

Urs Jenal

Publication

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Insert

cdGreen2.1

Expression

Bacterial

Promoter

Constitutive

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCDB179-TurboPETase

Plasmid

#218693

Purpose

Encodes codon optimized TurboPETase enzyme for bacterial expression

Depositor

Gregg Beckham

Publication

Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

Insert

TurboPETase

Tags

10xHis

Expression

Bacterial

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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HotPETase_pET21b

Plasmid

#215407

Purpose

Expression construct for HotPETase gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Publication

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

HotPETase

Expression

Bacterial

Mutation

S121E/D186H/R280A/P181V/S207R/S214Y/Q119K/S213E/N…

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET28a_Pro-IL-1β

Plasmid

#214340

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Publication

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL1B (IL1B Human)

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor_Tn[himar1C9]

Plasmid

#213906

Purpose

Mariner transposon with ATc-inducible himar1C9 transposase

Depositor

James Collins

Publication

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

mariner transposon with cam resistance and ATc-inducible himar1C9 transposase

Expression

Bacterial

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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M13-kmR

Plasmid

#206861

Purpose

M13KE from NEB modified with kanamycin resistance gene and NotI restriction site on g3p ( M13KE-NotI-Kan)

Depositor

Irene Chen

Publication

Lin et al PLoS One. 2011;6(5):e19991. doi: 10.1371

Insert

Kanamycin resistance gene

Expression

Bacterial

Promoter

lac

Available since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pVoPo-mSc

Plasmid

#187842

Purpose

Constitutive mScarlet-I-expression vector for Fusobacterium nucleatum

Depositor

Jörg Vogel

Publication

Ponath et al Proc Natl Acad Sci U S A. 2022 Oct 4;119

Insert

mScarlet-I

Expression

Bacterial

Available since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pSH424-ssr

Plasmid

#198026

Purpose

Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

Depositor

Susanne Häußler

Publication

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

T0 terminator - Sm resistance cassette

Expression

Bacterial

Promoter

SmR promoter

Available since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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PET-HiFi SpCas9-NLS-6xHis

Plasmid

#207376

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Publication

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

HiFi SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

R691A

Promoter

T7

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pET19-TdTwt

Plasmid

#207466

Purpose

Bacterial expression of Terminal deoxynucleotidyl transferase (TdT)

Depositor

Jay Keasling

Publication

Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

Insert

TdTwt (Dntt Mouse)

Tags

10xHis

Expression

Bacterial

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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UbC-blasticidine-P2A-TETon-3G transactivator

Plasmid

#200191

Purpose

A TETon activator, which combined with the DiLiCre 2.0 plasmid, drives the expression of DiLiCre 2.0 upon doxycycline. DiLiCre 2.0 is a cre recombinase that is activated by violet light.

Depositor

Jacco van Rheenen

Publication

Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:

Insert

blasticidine - P2A - TETon-3G transactivator

Use

Lentiviral

Expression

Bacterial and Mammalian

Promoter

UbC

Available since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRha-ABE8e-SpCas9-NG

Plasmid

#201190

Purpose

Expresses the base editor ABE8e-SpCas9-NG in bacterial cells

Depositor

Jonathan Yen

Publication

Mayuranathan et al Nat Genet. 2023 Jul;55(7):1210-1220. doi

Insert

ABE8e-SpCas9-NG

Tags

8xHIS and BP-NLS

Expression

Bacterial

Promoter

pRhaBAD

Available since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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