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pAIP4_4A02

Plasmid

#234194

Purpose

Heterologous protein expression of lytic chitin monooxygenase in Escherichia coli

Depositor

Christopher Bahl

Insert

4A02

Tags

10x HisTag-Smt3

Expression

Bacterial

Promoter

T7

Available Since

March 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pKSJ375

Plasmid

#103064

Purpose

Expresses ImmE1, immunity to colicin E1

Depositor

Alan Finkelstein, Karen Jakes

Article

Jakes et al J Bacteriol. 2016 Dec 13;199(1). pii: e0

Insert

colicin E1 immunity protein (pColE1_26, imm E. coli)

Expression

Bacterial

Promoter

E1 immunity protein promoter from ColE1

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pTE102

Plasmid

#59397

Purpose

MethyloBrick expression vector, mxaF promoter; TcR

Depositor

Tobias Erb

Article

Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

PmxaF

Available Since

Dec. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pMMLV-EGFP-P2A-Puro

Plasmid

#160226

Purpose

Expresses EGFP in mammalian cells

Depositor

Jason Sheltzer

Article

Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

Insert

EGFP-P2A-PuroR

Use

Retroviral

Expression

Bacterial and Mammalian

Promoter

LTR

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pMSP1E3D1_D73C

Plasmid

#39328

Depositor

Stephen Sligar

Article

Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

Insert

MSP1E3D1_D73C (APOA1 synthetic gene)

Tags

6xHis

Expression

Bacterial

Mutation

cys mutant of MSP1E3D1

Promoter

T7

Available Since

Sept. 11, 2012

Availability

Academic Institutions and Nonprofits only
pRSFDUET-1_VNp-anti-GFP mAb

Plasmid

#214955

Purpose

Bacterial expression of anti-GFP mAb light and heavy chain VNp fusions.

Depositor

Dan Mulvihill

Article

Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1

Inserts

VNp-anti-GFP mAb heavy chain
VNp-anti-GFP mAb light chain

Tags

VNp6

Expression

Bacterial

Promoter

T7

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCAH01Sp::Cas9

Plasmid

#128160

Purpose

Ptet-controlled expression of S. pyogenes Cas9

Depositor

Calvin Henard

Article

Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

Insert

Sp Cas9

Expression

Bacterial

Promoter

Ptet

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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TDP43-NTD

Plasmid

#241024

Purpose

Bacterial expression of TDP-43 NTD

Depositor

Nicolas Fawzi

Article

Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

Insert

TDP43-NTD

Tags

6x His

Expression

Bacterial

Mutation

changed N81 to stop codon

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pBR BsmAIM

Plasmid

#206206

Purpose

Constitutive expression of BsmAIM methyltransferase

Depositor

Andrew Ellington

Article

Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

Insert

BsamAIM

Expression

Bacterial

Available Since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET31b-T7-Spinach2

Plasmid

#79783

Purpose

Expresses Spinach2 under a T7 promoter

Depositor

Ming Hammond

Article

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Insert

T7-tRNA-Spinach2

Expression

Bacterial

Promoter

T7

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pUS200

Plasmid

#17788

Depositor

Stephen Sligar

Article

Unger et al J Biol Chem. 1986 Jan 25. 261(3):1158-63

Insert

Cytochrome P450cam

Expression

Bacterial

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
pET-28a(+)-GDH

Plasmid

#108954

Purpose

heterologous expression of GDH of B. megaterium in E. coli

Depositor

Frank Schulz

Article

Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

Insert

Glucose dehydrogenase IV (GDH IV)

Tags

His6-Tag

Expression

Bacterial

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pUWL201PW-creDE

Plasmid

#112241

Purpose

Streptomyces/E.coli shuttle expression vector encoding creD and creE

Depositor

Emily Balskus

Article

Waldman et al J Org Chem. 2018 May 29. doi: 10.1021/ac

Inserts

creD
creE

Expression

Bacterial

Available Since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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pJBL7044

Plasmid

#167215

Purpose

overexpression of PbrR in E. coli for cell-free lead sensing

Depositor

Julius Lucks

Article

Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

Insert

PbrR

Expression

Bacterial

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_mNeongreen

Plasmid

#182413

Purpose

Bacterial expression of mNeongreen

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

mNeongreen

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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gRNA3_NIPBL_Cas9-hGem-for_N-terminal_tagging

Plasmid

#217662

Purpose

expresses Cas9-hGem and guideRNA for N terminal tagging of NIPBL

Depositor

Daniel Gerlich

Article

Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

Insert

sgRNA3 for N terminal tagging of NIPBL (NIPBL Human)

Use

CRISPR

Expression

Bacterial and Mammalian

Promoter

U6

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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Nsp3 Mac1 - C2 (UCSF)

Plasmid

#169209

Purpose

Expression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removal

Depositor

James S Fraser

Article

Schuller et al Sci Adv. 2021 Apr 14;7(16). pii: 7/16/ea

Insert

SARS-CoV-2 Nsp3 Mac1

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

July 28, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pfa6a mScarlet-TsCC(B)::KANMX

Plasmid

#176397

Purpose

Plasmid for cloning mScarlet fused to TsCC(B) coiled coil into yeast.

Depositor

Matthew Good

Article

Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

Insert

mScarlet-TsCC(B)

Tags

mScarlet fused to TsCC(B)

Expression

Bacterial and Yeast

Promoter

none

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pMW41: pET-His-MBP-SUMO-R2Bm-SII

Plasmid

#209290

Purpose

Expresses the R2Bm ORF in E. coli

Depositor

Feng Zhang

Article

Wilkinson et al Science. 2023 Apr 21;380(6642):301-308.

Insert

R2

Tags

His, MBP, StrepII, and bdSUMO

Expression

Bacterial

Promoter

T7

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pProEx RhoA(G14C) CL

Plasmid

#224271

Purpose

Protein purification of RhoA (G14C)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

RhoA (RHOA Human)

Expression

Bacterial

Mutation

G14C

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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