Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Bacterial Expression Clear filter

Applied Filters

Bacterial Expression Clear filter

31,059 results

Showing: 4241 - 4260 of 31059 results

  4. peSCBE3-NG-Hypa

    Plasmid
    #218165
    Purpose
    This plasmid harbors the base editor eSCBE3-NG-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces
    Depositor
    Yuhui Sun
    Article
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    escbe3-NG-Hypa
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Expression
    Bacterial
    Mutation
    Mutation in the portion of deaminase hAPOBEC3A : …
    Promoter
    rpsL promoter
    Available Since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218165-plasmid-map-sequence-id-433201

    Enlarge

  5. pTNS2

    Plasmid
    #87802
    Purpose
    For constructing NusA-SUMO-fusion proteins with N-terminal StrepII tag and C-terminal His tag using "blunt-end" cloning.
    Depositor
    Yu-Zhu Zhang
    Article
    Zhang et al Mol Biotechnol. 2017 May;59(4-5):159-167
    Type
    Empty backbone
    Tags
    6xHis and Strep II
    Expression
    Bacterial
    Available Since
    May 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    87802-plasmid-map-sequence-id-177263

    Enlarge

  10. pPMQAK1-Ptrc-[E*](104)-Cas9-B0015-lacZ-PnrsB-mazF-LVA

    Plasmid
    #190790
    Purpose
    [E*](104) construct. Theophylline inducible Cas9 (S. pyogenes) expression. Two BsaI-sites allow for simultaneous insertion of sgRNA and donor DNA. Includes a nickel-inducible curing system.
    Depositor
    Paul Hudson
    Article
    Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102
    Inserts
    Cas9
    mazF-LVA
    Use
    CRISPR and Synthetic Biology
    Tags
    LVA
    Expression
    Bacterial
    Mutation
    silent mutations of BpiI-sites in original Cas9 g…
    Promoter
    PnrsB and Ptrc-[E*](104)
    Available Since
    Feb. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    190790-plasmid-map-sequence-id-384200

    Enlarge

  18. pCDF-RFP amber

    Plasmid
    #229524
    Purpose
    mCherry red fluorescent protein (RFP) reporter protein with an amber stop codon at position 13
    Depositor
    Thomas Huber
    Article
    Abdelkader et al Chem 102385
    Insert
    RFP amber
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    229524-plasmid-map-sequence-id-461664

    Enlarge

Showing: 4241 - 4260 of 31059 results