30,756 results

1. pET28a-His-hAos1

   Plasmid

   #53135

   Purpose

   E.coli expression of full length human Aos1 (SAE1) with Nterminal His-tag

   Depositor

   Frauke Melchior

   Article

   Pichler et al Cell. 2002 Jan 11;108(1):109-20.

   Insert

   Aos1 (SAE1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSC5GGv1

   Plasmid

   #194645

   Purpose

   pSC5 Golden-Gate Cloning Plasmid (BsaI - Site 1)

   Depositor

   Bogumil Karas

   Article

   Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

   Type

   Empty backbone

   Use

   Synthetic Biology; Diatom expression

   Expression

   Bacterial and Yeast

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGex-Cav1

   Plasmid

   #33364

   Depositor

   Ambra Pozzi

   Article

   Borza et al J Biol Chem. 2010 Dec 17;285(51):40114-2

   Insert

   Cav1 (CAV1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Dec. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pTCF7 HLA-B*07:02

   Plasmid

   #180446

   Purpose

   E. coli expression of soluble MHC protein

   Depositor

   John Altman

   Insert

   HLA-B*07:02
   

   Tags

   BSP41

   Expression

   Bacterial

   Mutation

   Replacement of transmembrane domain with enzymati…

   Available Since

   March 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pZac2.1 gfaABC1D-Lck-13xLinker-BioID2-BioID2-HA

   Plasmid

   #176741

   Purpose

   Expresses membrane bound biotin ligase, BioID2, in astrocytes

   Depositor

   Baljit Khakh

   Article

   Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

   Insert

   Lck-13xLinker-BioID2-BioID2-HA
   

   Use

   AAV

   Tags

   HA

   Expression

   Bacterial

   Promoter

   gfaABC1D

   Available Since

   April 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC-GFP-HS

   Plasmid

   #133305

   Purpose

   "Burdensome" GFP expressing plasmid carrying hok/sok toxin-antitoxin for plasmid stabilisation

   Depositor

   Chris Barnes

   Article

   Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1

   Insert

   hok/sok toxin-antitoxin cassette
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. LOV-Turbo-His6_pET21a

   Plasmid

   #199668

   Purpose

   expresses LOV-Turbo in bacteria

   Depositor

   Alice Ting

   Article

   Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

   Insert

   LOV-Turbo
   

   Tags

   His6

   Expression

   Bacterial

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRB035

   Plasmid

   #181944

   Purpose

   aTc-inducible expression of mNeonGreen

   Depositor

   Jeffrey Tabor

   Article

   Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

   Insert

   mNeonGreen; tetR (AX04_RS23485 Synthetic)
   

   Expression

   Bacterial

   Promoter

   mNG-PLtetO-1; tetR-J23106

   Available Since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET21 Catalytic Domain of MALT1

   Plasmid

   #48970

   Purpose

   Expresses catalytic domain of MALT1 in e.coli AA 329-566

   Depositor

   Guy Salvesen

   Article

   Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

   Insert

   MALT1 (MALT1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains AA 329-566

   Promoter

   T7

   Available Since

   Oct. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pMRE137

    Plasmid

    #118491

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mClover3
    

    Expression

    Bacterial

    Available Since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28_human-pro-IL18

    Plasmid

    #183390

    Purpose

    Overexpression of catalytic domain of inflammatory caspase in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    pro-IL18 (IL18 Human)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. PGEX-NEDD4

    Plasmid

    #45043

    Depositor

    Shiaw-Yih Lin

    Article

    Yim et al Cancer Cell. 2009 Apr 7;15(4):304-14. do

    Insert

    NEDD4-1 (NEDD4 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    June 6, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pY2ShHELIX_sgRNA_entry (CJT30)

    Plasmid

    #181781

    Purpose

    Expresses Y2-ShHELIX containing a nicking Y2 I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    Y2-nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
    

    Expression

    Bacterial

    Mutation

    Y2-nAniI = F13Y, S111Y, K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available Since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-PCNA-IRES-puro2b

    Plasmid

    #26461

    Depositor

    Daniel Gerlich

    Article

    Held et al Nat Methods. 2010 Sep . 7(9):747-54.

    Insert

    PCNA (PCNA Human)
    

    Tags

    pEGFP

    Expression

    Bacterial and Mammalian

    Available Since

    Dec. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX-4T-1 3xFlag-p38alpha

    Plasmid

    #47575

    Purpose

    Bacterial expression plasmid for GST-3xFlag-tagged p38alpha

    Depositor

    Kevin Janes

    Article

    Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

    Insert

    p38alpha (Mapk14 Mouse)
    

    Tags

    3xFlag and GST

    Expression

    Bacterial

    Available Since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pET24b-BR1.4

    Plasmid

    #199436

    Purpose

    expresses BAmRed1.4 in bacterial cells

    Depositor

    Andrew Woolley

    Article

    Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

    Insert

    BAMRed1.4
    

    Tags

    his tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. EF1m-ELP

    Plasmid

    #170763

    Purpose

    EF1m laminin α1 peptide fused to I30-ELP (VPGIG)30

    Depositor

    Andrew MacKay

    Article

    Truong et al Biomacromolecules. 2024 Jul 8;25(7):4001

    Insert

    EF1m-ELP
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMRS-AraC-GFP

    Plasmid

    #220199

    Purpose

    mini-Tn7 GFP expression vector - AraC/pBAD promoter

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    monomeric superfolder green fluorescent protein
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    monomeric superfolder green fluorescent protein

    Promoter

    AraC/pBAD

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMRS-XylS-GFP

    Plasmid

    #220200

    Purpose

    mini-Tn7 GFP expression vector - XylS/pM promoter

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    monomeric superfolder green fluorescent protein
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    monomeric superfolder green fluorescent protein

    Promoter

    XylS/pM

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJMP3666

    Plasmid

    #222352

    Purpose

    Expression vector with GFP expressed under PabstBR promoter, aprR marker, and replicates in A. baumannii and E. coli.

    Depositor

    Jason Peters

    Insert

    sfGFP
    

    Expression

    Bacterial

    Promoter

    PabstBR

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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