30,803 results

1. pnCS_SEPT7_SEPT9_i5-TEV-Strep

   Plasmid

   #174502

   Purpose

   bacterial co-expression of human SEPT7 and of human SEPT9_i5

   Depositor

   Manos Mavrakis

   Article

   Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

   Inserts

   SEPTIN7 (SEPTIN7 Human)
   SEPTIN9 (SEPTIN9 Human)
   

   Tags

   TEV-Strep

   Expression

   Bacterial

   Available Since

   Sept. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCrepusculo

   Plasmid

   #190156

   Purpose

   Regulates bacterial expression of target gene in blue-light-repressed manner. Based on Nakamurella multipartita PAL.

   Depositor

   Andreas Moeglich

   Article

   Ranzani et al ACS Synth Biol. 2022 Oct 21;11(10):3482-

   Type

   Empty backbone

   Tags

   His6 tag

   Expression

   Bacterial

   Available Since

   Nov. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJS169

   Plasmid

   #48882

   Purpose

   Negative feedback plasmid from oscillator

   Depositor

   Jeff Hasty

   Article

   Stricker et al Nature. 2008 Nov 27;456(7221):516-9. doi

   Insert

   lacI with ssrA tag
   

   Use

   Synthetic Biology

   Tags

   ssrA (TSAANDENYALAA)

   Expression

   Bacterial

   Promoter

   Para/lac

   Available Since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGNW4

   Plasmid

   #122088

   Purpose

   suicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cells

   Depositor

   Pablo Ivan Nikel

   Article

   Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJS167

   Plasmid

   #48881

   Purpose

   Positive feedback plasmid from oscillator

   Depositor

   Jeff Hasty

   Article

   Stricker et al Nature. 2008 Nov 27;456(7221):516-9. doi

   Inserts

   araC gene with ssrA tag
   monomeric yeast-enhanced green fluorescent protein
   

   Use

   Synthetic Biology

   Tags

   ssrA (TSAANDENYALAA)

   Expression

   Bacterial

   Promoter

   Para/lac

   Available Since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. TRBV19

   Plasmid

   #123453

   Purpose

   construction of TCR

   Depositor

   Catherine Wu

   Article

   Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

   Insert

   TRBV19 (TRBV19 Human)
   

   Expression

   Bacterial

   Mutation

   WT

   Available Since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET22b-csgA

   Plasmid

   #89581

   Purpose

   Express csgA curli protein with GS linker and Histaq

   Depositor

   Urartu Seker

   Article

   Abdelwahab et al Langmuir. 2017 Apr 7. doi: 10.1021/acs.l

   Insert

   csgA (csgA )
   

   Tags

   3xGGSG linker - TEV site - 3xGGSG linker and 6xHi…

   Expression

   Bacterial

   Available Since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28b_RanT24N

   Plasmid

   #186271

   Purpose

   Expression vector of His-RanT24N in E.coli

   Depositor

   Masahito Ikawa

   Article

   Mori et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

   Insert

   GTP-binding nuclear protein Ran (Ran Mouse)
   

   Tags

   His tagged and His-tagged at N terminal

   Expression

   Bacterial

   Mutation

   Changed T24 into N

   Promoter

   T7 promoter

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQTEV-ASF1A

   Plasmid

   #31591

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   ASF1 anti-silencing function 1 homolog A (S. cerevisiae) (ASF1A Human)
   

   Tags

   His and TEV

   Expression

   Bacterial

   Available Since

   Nov. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQE30-mBFP

    Plasmid

    #112682

    Purpose

    Expression of NADPH-dependent blue fluorescent protein

    Depositor

    Geun-Joong Kim

    Article

    Hwang et al Biochem Biophys Res Commun. 2012 Mar 23;

    Insert

    His-mBFP
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX6P-2-human RNF146 full length

    Plasmid

    #132610

    Purpose

    expresses human RNF146 full length in E. coli

    Depositor

    Wenqing Xu

    Article

    DaRosa et al Nature. 2015 Jan 8;517(7533):223-6. doi:

    Insert

    RNF146 (RNF146 Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. GST-YAP2

    Plasmid

    #24637

    Depositor

    Kunliang Guan

    Insert

    Yap2 (YAP1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Sept. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCold TDP43 M337V

    Plasmid

    #27453

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Tags

    Factor Xa and His6

    Expression

    Bacterial

    Mutation

    Methionine 337 mutated to Valine (M337V)

    Available Since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pRK603

    Plasmid

    #8831

    Depositor

    David Waugh

    Article

    Kapust et al Protein Expr Purif. 2000 Jul . 19(2):312

    Insert

    TEV protease, S219D mutant
    

    Expression

    Bacterial

    Mutation

    S219D mutant

    Available Since

    June 22, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. HHM-iATPSnFR1.1

    Plasmid

    #102547

    Purpose

    expresses ATP sensor in bacteria

    Depositor

    Baljit Khakh

    Article

    Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

    Insert

    iATPSnFR1.1
    

    Expression

    Bacterial

    Available Since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAJM.847

    Plasmid

    #108524

    Purpose

    PhlFAM + PPhlF-YFP Reporter. DAPG sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    PhlFAM + PPhlF-YFP
    

    Expression

    Bacterial

    Mutation

    PhlF: S50G S73I R95H A121V M137I N144D T155I R200C

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. Map3k1

    Plasmid

    #29581

    Depositor

    Dustin Maly

    Article

    Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

    Insert

    Map3k1 (MAP3K1 Human)
    

    Tags

    GST, HIS6, and TEV

    Expression

    Bacterial

    Mutation

    Constitutively Active. Contains catalytic domain …

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pGEMTEZ-TeTxLC

    Plasmid

    #32640

    Depositor

    Richard Axel, Joseph Gogos, C. Ron Yu

    Article

    Yu et al Neuron. 2004 May 27;42(4):553-66.

    Insert

    Tetanus toxin light chain
    

    Expression

    Bacterial

    Available Since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-His-VCre

    Plasmid

    #123131

    Purpose

    Bacterial expression plasmid for wild-type VCre with an N-terminal His-tag.

    Depositor

    David Liu

    Article

    Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

    Insert

    VCre recombinase
    

    Use

    Cre/Lox

    Tags

    6x His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28b-hUba2-His

    Plasmid

    #53117

    Purpose

    expression of full length human Uba2 with Cterminal His-tag in E.coli

    Depositor

    Frauke Melchior

    Article

    Werner et al Methods Mol Biol. 2009;497:187-99. doi:

    Insert

    UBA2 (UBA2 Human)
    

    Tags

    His and T7 tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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