29,771 results

1. pET23b-Casp7-His

   Plasmid

   #11825

   Depositor

   Guy Salvesen

   Article

   Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

   Insert

   Caspase 7 (CASP7 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 12, 2006

   Availability

   Academic Institutions and Nonprofits only

2. pDonor14-ShoHELIX-KanR (BO4)

   Plasmid

   #181795

   Purpose

   pDonor for ShoHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargo

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   KanR, R6K origin of replication
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Ubiquitin K48C

   Plasmid

   #86588

   Purpose

   Bacterial expression of Ubiquitin K48C for cross linking studies

   Depositor

   Sharon Rozovsky

   Article

   Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

   Insert

   Ubiquitin (UBC Human)
   

   Use

   Tags

   His6-MBP

   Expression

   Bacterial

   Mutation

   TEV site; K48C

   Promoter

   Tac

   Available since

   Feb. 24, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. EGFP-p300 (D1399Y)

   Plasmid

   #191763

   Purpose

   Expression of EGFP fused to catalytically dead core p300 histone acetyltransferase (D1399Y)

   Depositor

   Daniel Gerlich

   Article

   Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

   Insert

   FRB-EGFP-p300 core (D1399Y) (EP300 Human)
   

   Use

   Tags

   EGFP (N-terminal of p300 core (D1399Y)) and FRB (…

   Expression

   Bacterial and Mammalian

   Mutation

   catalytic D1399Y mutation in p300

   Promoter

   EF1alpha

   Available since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDAI-SceI

   Plasmid

   #32061

   Purpose

   Construction of unmarked gene deletions. Broad host range replicative plasmid expressing the I-SceI homing endonuclease

   Depositor

   Miguel Valvano

   Article

   Flannagan et al Environ Microbiol. 2008 Jun;10(6):1652-6

   Insert

   I-SceI nuclease
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pdhfr

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDM026

   Plasmid

   #216808

   Purpose

   Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and pyrG selectable marker.

   Depositor

   Ken Sawin

   Article

   Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

   Type

   Empty backbone

   Use

   CRISPR; Fungal expression

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Lbpro (29-195)

   Plasmid

   #110759

   Purpose

   Bacterial expression of Lbpro

   Depositor

   Tim Skern

   Article

   Guarne et al EMBO J. 1998 Dec 15;17(24):7469-79. doi:

   Insert

   Lb pro
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pESUMO-hRORg1

   Plasmid

   #170330

   Purpose

   Expresses RORg isoform 1 with N-terminal HisSUMO tag in bacteria

   Depositor

   Patrick Griffin

   Article

   Strutzenberg et al J Mol Biol. 2021 Sep 20;433(22):167258.

   Insert

   RORG isoform 1 (RORC Human)
   

   Use

   Tags

   HisSUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCR-II-TOPO-mitfa_LexPR-2A-Cerulean-SV40pA-FRT-Kan-FRT: Sequences,

   Plasmid

   #110201

   Purpose

   Melanocyte-specific expression of the LexPR-2A-Cerulean fusion

   Depositor

   Tatjana Sauka-Spengler

   Article

   Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

   Insert

   LexPR transactivator - 2A - Cerulean
   

   Use

   Tags

   Cerulean

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJBL3752

    Plasmid

    #128809

    Purpose

    Fluoride riboswitch-regulated sfGFP

    Depositor

    Julius Lucks

    Article

    Thavarajah et al ACS Synth Biol. 2019 Dec 12. doi: 10.102

    Insert

    Fluoride riboswitch-regulated sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBA615

    Plasmid

    #186239

    Purpose

    eLbuCas13a Negative Control (RFP targeting)

    Depositor

    Jennifer Doudna

    Article

    Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

    Insert

    pTet-LbuCas13a + crRNA negative control
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28C-SARS-CoV-2-Nucleocapsid

    Plasmid

    #204402

    Purpose

    E. coli expression of full-length SARS-CoV-2 nucleocapsid

    Depositor

    Samie Jaffrey

    Article

    Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

    Insert

    SARS-CoV-2 nucleocapsid (N SARS-CoV-2)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSG 25

    Plasmid

    #63867

    Purpose

    Expression of beta-galactosidase at high levels in E. coli

    Depositor

    Albert Dahlberg

    Article

    O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

    Insert

    lacZ (lacZ Escherichia coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PtacI

    Available since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pDEST17-UbE2E1

    Plasmid

    #15787

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2E1 (UBE2E1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pCold TDP43 Q331K

    Plasmid

    #27454

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    Factor Xa and His6

    Expression

    Bacterial

    Mutation

    Glutamine 331 mutated to Lysine (Q331K)

    Promoter

    Available since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pMRBAD-Z-CGFP

    Plasmid

    #52735

    Purpose

    L-(+)-arabinose-inducible expression of Z-CGFP positive control for in vivo split GFP assembly assay

    Depositor

    Lynne Regan

    Article

    Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57

    Insert

    Z+CGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    May 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR221-SAR1-no stop

    Plasmid

    #67345

    Purpose

    hSar1 without stop codon in Gateway Entry vector

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    SAR1 (SAR1A Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    None

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pTW199b2

    Plasmid

    #136928

    Purpose

    Directed evolution of SpCas9

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    gIII
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBSV2G_PflaB-msfGFPBb

    Plasmid

    #118231

    Purpose

    Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven msfGFP

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

    Insert

    flagellin promoter driven msfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBT1-proD-mCherry

    Plasmid

    #65823

    Purpose

    Const. mCherry expression , proD promoter, E. coli Expression

    Depositor

    Michael Lynch

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    proD E . coli Constitutive Promoter

    Available since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge