32,408 results
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Plasmid#196366Purposeprotein expression of the His-tagged full length proteinDepositorInsertBIRC3/cIAP2
TagsHis6-TEVExpressionBacterialPromoterT7Available SinceFeb. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBRα-β flap (831-1057)
Plasmid#53734PurposeExpression of alpha NTD fused via three alanines to E. coli RNAP subunit beta, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.DepositorInsertalpha NTD-Beta flap fusion
ExpressionBacterialPromoterlpp/lacUV5 (tandem promoter)Available SinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBbA5k-acrAB-sfGFP
Plasmid#195516PurposeInducible expression vector for transcriptionally fused acrAB and sfGFP.DepositorInsertacrAB
UseSynthetic BiologyTagssfgfpExpressionBacterialPromoterlacUV5Available SinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMCRW1
Plasmid#122462PurposeHuman NPM-ALK fusion protein gene in pET28a bacterial expression plasmid. Production of recombinant human NPM-ALK fusion protein in E. coli Bl21 Rosetta2 cellsDepositorTagsHis TagExpressionBacterialPromoterT7lac promoterAvailable SinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
dCas9-CRY2Clust-FUSN
Plasmid#200967Purposeinduction of phase-separated condensate mediated by FUSN-IDRDepositorInsertFUSN
UseLentiviralExpressionBacterial and MammalianAvailable SinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOG231
Plasmid#17736DepositorInsertNLS-Cre recombinase
UseCre/LoxExpressionBacterialAvailable SinceApril 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
PJQ49
Plasmid#134657PurposeContains Promoter 3 with bsa1 cut sites for inserting sgRNADepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterAnderson BBa_J23115Available SinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-HRas-1-167-WT
Plasmid#245306PurposeExpresses G-domain of human HRas (residues 1-167 wild type) in E. coliDepositorInsertHRas (HRAS Human)
Tags6HisExpressionBacterialMutationdeleted amino acids 168-189PromoterT7lacAvailable SinceOct. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(4/5FTrp)RS
Plasmid#207620PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 4-Fluoro-L-Tryptophan (4FTrp) and 5-Fluoro-L-Tryptophan (5FTrp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
ExpressionBacterialPromoterT7Available SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-PA-Rac1-T17N
Plasmid#22029PurposeExpression of photoactivatable Rac1 (dominant-negative mutant).DepositorInsertPA-Rac1-T17N (RAC1 Avena sativa (oat), Human)
Tags6xHis and mCherryExpressionBacterial, Insect, and Mamm…MutationLOV(Leu404-Leu546). Rac1 starts at Ile4 and conta…PromoterCMV, p10Available SinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET28-His6-Ubc9
Plasmid#133909PurposeBacterial expression of His6-Ubc9DepositorInsertSUMO-conjugating enzyme UBC9 (UBE2I Human)
TagsThrombin-cleavable His6-tagExpressionBacterialPromoterT7Available SinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJYS2cm_crtYf
Plasmid#85543PurposeConstitutive transcription of crRNA of crtYf, Chloramphenicol resistanceDepositorInsertcrRNA of crtYf
UseCRISPR; Shuttle vector corynebacterium glutamicum…ExpressionBacterialAvailable SinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJJS002
Plasmid#188325PurposePCR template for repair templates to tag a gene with a GFP and mIAA7 AID degron using homology-directed repair for C. elegans.DepositorInsertLinker::GFP::mIAA7
ExpressionBacterialAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBad-PhoCl2f
Plasmid#164036PurposeImproved PhoCl variant with faster dissociation rateDepositorInsertPhoCl2f
TagsHis tag, T7 tag, Xpress TagExpressionBacterialPromoteraraBad promotorAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-5FLAG-KanMX6
Plasmid#15983DepositorTypeEmpty backboneTags5xFlagExpressionBacterialAvailable SinceOct. 31, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-PCNA-IRES-puro2b
Plasmid#26461DepositorAvailable SinceDec. 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PHGDH
Plasmid#74113PurposeExpression of N-terminal His-taggedDepositorAvailable SinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-6His-MBP-MpAgo
Plasmid#79249PurposeThis plasmid encodes the Argonaute protein from Marinitoga Piezophila and is codon optimized for E. coli expressionDepositorInsertMarinitoga piezophila Argonaute
UseCRISPRTags6XHis-tag + MBP + PreScission Cleavage SiteExpressionBacterialPromoterT7Available SinceJuly 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTn7-SCOUT11.G
Plasmid#200999PurposepLv1-pUC18R6KT-miniTn7-Gm-sfGFP, GmR, ApRDepositorInsertsuperfolder GFP
ExpressionBacterialPromoterJ23104Available SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHMattR3-attR4-GFP
Plasmid#196247PurposepHM1 derived plasmid containing the Gateway cassette attR3-attR4 and GFP geneDepositorTypeEmpty backboneExpressionBacterialAvailable SinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only