31,586 results
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Plasmid#163385Purpose2nd generation lentiviral vector to express sHalotag-sGFP module in the G-baToN systemDepositorInsertHalotag-GFP-PDGFRtm-2A-mCherry (HaloGPM)
UseLentiviralTagsmCherryExpressionBacterial and MammalianPromoterEF1aAvailable SinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-hph-Ptac
Plasmid#218364PurposeEmpty backbone for Tn7-site insertion with lacIq-Ptac expression systemDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSNK
Plasmid#135193PurposeArabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains kamamycin resistance cassetteDepositorInsertI-SceI lamba Red
UseSynthetic BiologyExpressionBacterialMutationNonePromoteraraBADAvailable SinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTECH-MbAcK3RS(IPYE)
Plasmid#104070PurposeExpression plasmid encoding MbAcK3RS(IPYE) and pylTDepositorInsertPlpp MbAcK3RS(IPYE); PproK pyl
ExpressionBacterialMutationContains activating mutations V31I, T56P, H62Y, A…PromoterPlpp and PproKAvailable SinceDec. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mAmetrine-CNA35
Plasmid#61604Purposebacterial expression of N-terminal fusion protein of CNA35 with mAmetrineDepositorInsertmAmetrine and CNA35
TagsHis-tagExpressionBacterialPromoterT7Available SinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pAC-Kan-alphaGal4
Plasmid#13421DepositorInsertRNAP alpha-Gal4
ExpressionBacterialAvailable SinceFeb. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMJH65
Plasmid#67273PurposeConjugally transferable red recombinogenic plasmidDepositorInserttetA
UseRed recombinase, temperature sensitiveExpressionBacterialPromotertetA promoter of plasmid pACYC184 originAvailable SinceSept. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET151-GFP-PP-RPN2 (940-953)
Plasmid#73746PurposeExpression of GFP-PP-RPN2 (940-953) fusion in E.coliDepositorInsertRPN2 (RPN2 Human)
Tags6x His and GFPExpressionBacterialMutationConstruct possesses only residues 940-953 of RPN2PromoterT7Available SinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-His6-SUMO-MproC145A
Plasmid#172653PurposeBacterial expression of SARS-CoV-2 Mpro C145A inactive mutant fused to SUMODepositorInsertSARS-CoV-2 Mpro (ORF1ab Severe acute respiratory syndrome coronavirus 2)
Tags6xHis Tag and SUMOExpressionBacterialMutationCatalytic Cysteine 145 mutated to AlaninePromoterT7Available SinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCoofy1
Plasmid#43974DepositorTypeEmpty backboneTagsHis6ExpressionBacterialPromoterT7Available SinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEF1-Pls4-sfGFP
Plasmid#205751PurposeExpresses sfGFP under IPTG inducible promoter Pls4 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. High strength.DepositorTypeEmpty backboneUseShuttle vector gram+ gram-ExpressionBacterialPromoterPls4 (Phyperspank mutant, IPTG inducible)Available SinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKEK3230
Plasmid#234102PurposeCCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.DepositorInsertFuGFP
ExpressionBacterialPromoterpJ23100-BsaIAvailable SinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBad33_IS605_reRNA
Plasmid#205321PurposeBacterial expression of IS605 TnpB and its reRNADepositorInsertIS605 TnpB
ExpressionBacterialAvailable SinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
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ATX3-13Q
Plasmid#184248PurposeRecombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal)DepositorInsertataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track (ATXN3 Synthetic, Human)
Tags6x His-Tag and TEV cleavage siteExpressionBacterialMutationC-terminal codon optimization for protein expres…PromoterT7 promoterAvailable SinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PFN1_Profilin
Plasmid#110028PurposeProtein expression and purification of PFN1_ProfilinDepositorInsertPFN1_Profilin (PFN1 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRaGE Pyl TAG GFP Y35TAG
Plasmid#160041PurposeEncodes for MmPyl tRNA synthetase/tRNA pair and for GFP reporter with TAG mutation at site 35, for unnatural amino acid incorporation into the GFP protein in Vibrio natriegens.DepositorInsertsPyrrolysyl tRNA synthetase (Methanosarcina mazei)
Pyrrolysyl tRNA(cua) (Methanosarcina mazei)
deGFP
UseSynthetic BiologyTagsHis-tagExpressionBacterialMutationChanged tyrosine 35 to TAG stop-codon (Site numbe…PromoterP70b promoter and T500 terminator, Vibrio natrieg…Available SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1-6AlanineHE1
Plasmid#165098Purposebacterial expression of a GST fusion of Human Uba1 with a 6alanine linker between the GST encoded by the plasmid and the inserted Uba1DepositorAvailable SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
SPS-6803
Plasmid#80458PurposeIPTG inducible expressoin of sucrose phosphate synthase derived from Synechocystis sp. PCC6803DepositorInsertSucrose phosphate synthase
ExpressionBacterialAvailable SinceOct. 25, 2016AvailabilityAcademic Institutions and Nonprofits only