30,261 results

1. pDEST17-hTRPT1-iso1

   Plasmid

   #184239

   Purpose

   For purification of N-terminally His-tagged TRPT1 from bacteria

   Depositor

   Karla Feijs

   Article

   Weixler et al Nucleic Acids Res. 2022 Sep 9;50(16):942

   Insert

   TRPT1 (TRPT1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRC072

   Plasmid

   #225982

   Purpose

   J23110_buj_sfGFP_buj_mRFP

   Depositor

   Jesse Zalatan

   Article

   Cardiff et al Nucleic Acids Res. 2024 May 22;52(9):540

   Insert

   sfGFP (with buj RBS), mRFP (with buj RBS)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23110

   Available since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA6.MCV.LT339.V5.4

   Plasmid

   #28193

   Depositor

   Patrick Moore

   Article

   Shuda et al Proc Natl Acad Sci U S A. 2008 Oct 21. 1

   Insert

   MCPyV Truncated Tumor Large T antigen (399 tumor strain)
   

   Use

   Tags

   V5

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Oct. 3, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOPINA

   Plasmid

   #41141

   Depositor

   Ray Owens

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRSET.GltI253-cpGFP.L1LV/L2NP

   Plasmid

   #41733

   Purpose

   a single-wavelength intracellular glutamate sensor constructed from E. coli Gltl and cpGFP. For bacterial cytoplasmic expression

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

   Insert

   GltI based Glutamate Biosensor
   

   Use

   Glutamate biosensor

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Bacterial

   Mutation

   cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…

   Promoter

   T7

   Available since

   Jan. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAJM.1642

   Plasmid

   #108535

   Purpose

   CinRAM + PCin-YFP Reporter. OHC14 sensor for use in non-Marionette strain.

   Depositor

   Christopher Voigt

   Article

   Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

   Insert

   CinRAM + PCin-YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   CinR: S72C V80I G114D G115D R137H K220R A224T *24…

   Promoter

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX6p-1-BCCP-PupE

   Plasmid

   #113403

   Purpose

   Expresses BCCP-PupE in bacteria

   Depositor

   Min Zhuang

   Article

   Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

   Insert

   BCCP-PupE
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Yellow Cameleon-Nano15/pBig

   Plasmid

   #51962

   Purpose

   Expression of Yellow Cameleon-Nano15, an ultrasensitive Ca2+ indicator with Kd = 15 nM, in Dictyostelium

   Depositor

   Takeharu Nagai

   Article

   Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

   Insert

   Yellow Cameleon-Nano15
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTCF6 HLA-A*24:02

   Plasmid

   #180445

   Purpose

   E. coli expression of soluble MHC protein

   Depositor

   John Altman

   Insert

   HLA-A*24:02
   

   Use

   Tags

   BSP41

   Expression

   Bacterial

   Mutation

   Replacement of transmembrane domain with enzymati…

   Promoter

   Available since

   March 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28_hTLN2-FL

    Plasmid

    #164835

    Purpose

    Expression of FL human talin2

    Depositor

    Tina Izard

    Article

    Rangarajan et al J Biol Chem. 2020 Sep 11;295(37):12885-1

    Insert

    TLN2 (TLN2 Human)
    

    Use

    Tags

    His8

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBS PKC theta

    Plasmid

    #8426

    Depositor

    Frederic Mushinski

    Article

    Mischak et al FEBS Lett 1993 Jul 12;326(1-3):51-5.

    Insert

    Mouse Protein Kinase C theta (Prkcq Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 22, 2005

    Availability

    Academic Institutions and Nonprofits only

12. pAL-pck_T

    Plasmid

    #74068

    Purpose

    pAL374 plasmid carrying the pck (T) sgRNA targeting, the template strand of pck, SpecR

    Depositor

    Timothy Lu

    Article

    Cleto et al ACS Synth Biol. 2016 Feb 16.

    Insert

    sgRNA pck (T)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ptac

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pMUT2-pCadBA-mCherry

    Plasmid

    #192865

    Purpose

    pMUT2 based vector for sensing pH in E. coli Nissle 1917

    Depositor

    Morten Sommer

    Article

    Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

    Insert

    pH sensitive promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTXB1-TP5

    Plasmid

    #160088

    Purpose

    Bacterial expression of Tn5-APEX2 fusion protein with linker GSGAGA

    Depositor

    Pier Pandolfi

    Article

    Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

    Insert

    Tn5 transposase/APEX2 peroxidase fusion protein
    

    Use

    Tags

    FLAG and Mxe intein - Chitin-binding domain

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPSV39-ugi

    Plasmid

    #186556

    Purpose

    Broad host range inducible expression of uracil-DNA glycosylase inhibitor (UGI)

    Depositor

    Joseph Mougous

    Article

    Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

    Insert

    ugi
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    LacUV5

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNB22

    Plasmid

    #103059

    Purpose

    medium copy plasmid (pBR322 backbone) expressing the unstable GFP variant GFP-OVA under control of the nitric oxide-inducible promoter hmpAp

    Depositor

    Dirk Bumann

    Article

    Burton et al Cell Host Microbe. 2014 Jan 15;15(1):72-

    Insert

    GFP-OVA
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    Promoter

    hmpAp

    Available since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pYFJ16-LplA(W37V) (for E. Coli expression)

    Plasmid

    #34838

    Depositor

    Alice Ting

    Article

    Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

    Insert

    LplA W37V (lplA E. Coli)
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Mutated Trp37 to Val

    Promoter

    T5 promoter

    Available since

    May 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTXB1-TP3

    Plasmid

    #160086

    Purpose

    Bacterial expression of Tn5-APEX2 fusion protein with linker AEAAAKEAAAKA

    Depositor

    Pier Pandolfi

    Article

    Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

    Insert

    Tn5 transposase/APEX2 peroxidase fusion protein
    

    Use

    Tags

    FLAG and Mxe intein - Chitin-binding domain

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGCP123-EbpA_g1

    Plasmid

    #153517

    Purpose

    sgRNA for ebpA gene (NT) under nisin-inducible nisA promoter, barcoded

    Depositor

    Kimberly Kline

    Article

    Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

    Insert

    pnisA-sgRNA(EbpA_g1)-dCas9 scaffold
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBAD/His-miRFP703

    Plasmid

    #79985

    Purpose

    Monomeric near-infrared fluorescent protein miRFP703 in bacterial plasmid

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

    Insert

    miRFP703
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araB

    Available since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge