30,803 results

1. pBT13-splitD

   Plasmid

   #122597

   Purpose

   clone M13 phage by SapI Golden Gate

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   M13 bacteriophage genes
   

   Expression

   Bacterial

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMB413a

   Plasmid

   #20058

   Depositor

   Stephen Sligar

   Article

   Springer et al Proc Natl Acad Sci U S A. 1987 Dec . 84(

   Insert

   Sperm Whale Myoglobin
   

   Expression

   Bacterial

   Available Since

   Jan. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

3. pET303-colE1T

   Plasmid

   #155180

   Purpose

   Expresses colicin E1 residues 1-190

   Depositor

   Joanna Slusky

   Article

   Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

   Insert

   colicin E1 residues 1-190
   

   Tags

   C-terminal 6x Histidine tag

   Expression

   Bacterial

   Mutation

   truncation of colicin E1 including 1-190

   Promoter

   T7

   Available Since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. ATG-3925 (CBG2)

   Plasmid

   #167839

   Purpose

   Expresses CBG2 (Click Beetle Green 2) in E. coli and mammalian cells

   Depositor

   Lance Encell

   Article

   Zambito et al iScience. 2020 Dec 26;24(1):101986. doi:

   Insert

   Click Beetle Green 2
   

   Tags

   6-His

   Expression

   Bacterial and Mammalian

   Mutation

   Q348H. Click Beetle Green 2 (CBR2) has 3 amino a…

   Promoter

   CMV, T7

   Available Since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pnEA-vH_His-TEV-SEPT2-msfGFP_SEPT6

   Plasmid

   #174498

   Purpose

   bacterial co-expression of human SEPT2 fused to monomeric superfolder GFP and of human SEPT6

   Depositor

   Manos Mavrakis

   Article

   Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

   Inserts

   SEPTIN2 (SEPTIN2 Human)
   SEPTIN6 (SEPTIN6 Human)
   

   Tags

   His6-TEV and msfGFP

   Expression

   Bacterial

   Available Since

   Sept. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pOPINB-hRNF216

   Plasmid

   #167354

   Purpose

   Expression of human RNF216 (Triad3A) codon optimized for E. coli and insect cells

   Depositor

   Bernhard Lechtenberg

   Article

   Cotton et al Mol Cell. 2022 Feb 3;82(3):598-615.e8. d

   Insert

   E3 ubiquitin-protein ligase RNF216 (RNF216 Human)
   

   Tags

   3C protease cleavage site and 6x-His tag

   Expression

   Bacterial

   Mutation

   codon optimised for expression in E. coli

   Promoter

   T7

   Available Since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28CLY3

   Plasmid

   #21762

   Depositor

   Maarten Merkx

   Article

   Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

   Insert

   ECFP -EYFP linked by flexible linker containing 3 SGGSGG repeats
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Dec. 1, 2009

   Availability

   Academic Institutions and Nonprofits only

8. pFA6a-hphMX6-3nmt1

   Plasmid

   #105162

   Purpose

   Yeast gene targeting

   Depositor

   Jian-Qiu Wu

   Article

   Ye et al Mol Biol Cell. 2012 Apr;23(7):1181-95. d

   Insert

   hphMX6-3nmt1
   

   Tags

   3nmt1

   Expression

   Bacterial and Yeast

   Promoter

   3nmt1

   Available Since

   Feb. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pST50Trc2-HISNDHFR

   Plasmid

   #63958

   Purpose

   Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Tags

   6xHis; TEV cleavable

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBS mNotch1-IC (DM#270)

    Plasmid

    #15079

    Depositor

    Douglas Melton

    Article

    Murtaugh et al Proc Natl Acad Sci U S A. 2003 Dec 9. 10

    Insert

    Notch1-IC (Notch1 Mouse)
    

    Expression

    Bacterial

    Mutation

    K1750A

    Available Since

    Oct. 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET-EIN-AlkBH5(66-292)

    Plasmid

    #125732

    Purpose

    Plasmid with EIN as a solubility and expression enhancing tag to increase production of hard to express proteins such as AlkBH5 and TEV cleaving site for the removal of the fusion tag.

    Depositor

    Vincenzo Venditti

    Article

    Khatiwada et al Protein Expr Purif. 2019 Nov 15;167:1055

    Insert

    AlkBH5(66-292) (ALKBH5 Human)
    

    Tags

    6xHis and EIN

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. NAT1

    Plasmid

    #25511

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    NAT1 (NAT1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 30, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pYTK083

    Plasmid

    #65190

    Purpose

    Encodes AmpR-ColE1 as a Type 8 part to be used in the Dueber YTK system

    Depositor

    John Dueber

    Article

    Lee et al ACS Synth Biol. 2015 Apr 14.

    Insert

    AmpR-ColE1
    

    Expression

    Bacterial

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET-21a-PE2-His

    Plasmid

    #201973

    Purpose

    Express His-tagged PE2 in bacteria

    Depositor

    Scot Wolfe

    Article

    Liang et al Nat Methods. 2023 Jun;20(6):898-907. doi

    Insert

    PE2-HIS
    

    Tags

    His and c-myc NLS

    Expression

    Bacterial

    Available Since

    June 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PIK3C2GA

    Plasmid

    #42451

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PIK3C2GA (PIK3C2G Human)
    

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    See Comments

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pBEST-OR2-OR1-Pr-araC, pBAD-TetR, pBAD-TetO1-deGFP-ssrA

    Plasmid

    #45789

    Depositor

    Richard Murray

    Inserts

    deGFP
    Tetracycline repressor
    araC
    

    Use

    Synthetic Biology

    Tags

    ssrA degradation tag

    Expression

    Bacterial

    Promoter

    OR2-OR1-Pr (bacteriophage Lambda with one mutatio…

    Available Since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAM5087

    Plasmid

    #85123

    Purpose

    Ptrc-YFP subcloned from pAM5080 into NSII-Km vector.

    Depositor

    Susan Golden

    Article

    Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d

    Insert

    YFP
    

    Expression

    Bacterial

    Available Since

    June 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CAGGS-MCS-NLS-3XFLAG-V5

    Plasmid

    #196383

    Purpose

    Empty backbone vector to insert cDNA for strong expression in mammalian cells

    Depositor

    Mauro Calabrese

    Article

    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

    Type

    Empty backbone

    Tags

    3xFLAG and V5

    Expression

    Bacterial and Mammalian

    Promoter

    CAGGs

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET_MBP-RGG-RGG

    Plasmid

    #124946

    Purpose

    Expression of MBP-RGG-RGG

    Depositor

    Matthew Good, Daniel Hammer, Benjamin Schuster

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    MBP-RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Tags

    His and MBP

    Expression

    Bacterial

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMV306G13+FFlucRT

    Plasmid

    #49997

    Purpose

    Integrating vector with an improved Red-shifted Thermostable Firefly Luciferase

    Depositor

    Brian Robertson

    Article

    Andreu et al J Antimicrob Chemother. 2013 Sep;68(9):2

    Insert

    G13 promoter + Firefly luciferase Red Thermostable
    

    Use

    Mycobacteria integrating vector

    Expression

    Bacterial

    Mutation

    Codon optimized for Mycobacterium tuberculosis wi…

    Promoter

    G13 from M. marinum

    Available Since

    May 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge