30,804 results

1. pBbE5a-Opto-Cre-Vvd

   Plasmid

   #134404

   Purpose

   Light-inducible Cre recombinase split with Vivid photodimers for bacterial expression.

   Depositor

   Mary Dunlop

   Article

   Sheets et al ACS Synth Biol. 2020 Feb 21;9(2):227-235

   Insert

   Opto-Cre-Vvd
   

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Bacterial

   Promoter

   lacUV5

   Available Since

   Jan. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. GST-PRMT1

   Plasmid

   #34699

   Depositor

   Steven Clarke

   Article

   Gary et al J Biol Chem. 1996 May 24;271(21):12585-9

   Insert

   PRMT1 (Prmt1 Rat)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Jan. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pMM659

   Plasmid

   #68888

   Purpose

   Chondroitin sulfate-inducible NanoLuc expression, pNBU2 backbone, AmpR

   Depositor

   Timothy Lu, Christopher Voigt

   Article

   Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

   Insert

   NanoLuc
   

   Expression

   Bacterial

   Available Since

   Oct. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a HAT epitope

   Plasmid

   #129445

   Purpose

   expression of HAT epitope

   Depositor

   Oskar Laur

   Insert

   HAT epitope
   

   Expression

   Bacterial

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-(-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis

   Plasmid

   #62373

   Purpose

   Expression of (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis
   

   Tags

   (-30)dGFP, 6xHis tag, Cathepsin cleavage site, an…

   Expression

   Bacterial

   Mutation

   Y67S amino acid substitution in (-30)GFP renders …

   Promoter

   T7

   Available Since

   June 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. Mouse ZP3 promoter (JD#445)

   Plasmid

   #14634

   Depositor

   Jurrien Dean

   Article

   Rankin et al Development. 1998 Jul . 125(13):2415-24.

   Insert

   ZP3 promoter (Zp3 Mouse)
   

   Expression

   Bacterial

   Available Since

   March 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEB2-mCherry2-L

   Plasmid

   #104003

   Purpose

   Plasmid encoding mCherry2-L

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   mCherry2-L
   

   Use

   Low copy

   Expression

   Bacterial

   Mutation

   Mutations relative to DsRed (MSKGEE NNLA IIKEF… V…

   Promoter

   proC

   Available Since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBtPIPLC His

   Plasmid

   #173808

   Purpose

   Expression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cells

   Depositor

   Anne Gershenson, Mary F Roberts

   Article

   He et al J Biol Chem. 2015 Jul 31;290(31):19334-4

   Insert

   Bacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
   

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7

   Available Since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHH0103_ESRRB_Hormone-recep

   Plasmid

   #109909

   Purpose

   Protein expression and purification of ESRRB_Hormone-recep

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   ESRRB_Hormone-recep (ESRRB Human)
   

   Expression

   Bacterial

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pS3

    Plasmid

    #50596

    Purpose

    Backbone: pBbB5c Origin Of Replication:pBBR1 Selection Markers: Chloramphenicol Promoters: PLacUV5

    Depositor

    Jay Keasling

    Article

    Juminaga et al Appl Environ Microbiol. 2012 Jan;78(1):8

    Inserts

    aroE
    aroD
    aroB
    aroG*
    ppsA
    tktA
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    lacUV5

    Available Since

    Oct. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSC101-GFPmut2-mScarlet-I TAA

    Plasmid

    #208187

    Purpose

    nonsense mistranslation, TAA stop

    Depositor

    Tanel Tenson

    Article

    Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

    Inserts

    GFPmut2
    mScarlet-I
    

    Use

    Reporter

    Expression

    Bacterial

    Mutation

    Insertion 6TAA*(stop) 7AGT(Ser)

    Promoter

    Ptet+dnaK P1

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQC

    Plasmid

    #48100

    Purpose

    Cumate-inducible expression of ECFP-tagged fusion protein

    Depositor

    Julia Vorholt

    Article

    Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

    Type

    Empty backbone

    Tags

    3xFLAG and ECFP

    Expression

    Bacterial

    Promoter

    PQ5

    Available Since

    Jan. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDG04439

    Plasmid

    #216365

    Purpose

    xhNup133-Nb2t (+2Cys) Nanobody production

    Depositor

    Dirk Görlich

    Article

    Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

    Insert

    xhNup133-Nb2t (+2Cys)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    14xHis-NEDD8

    Expression

    Bacterial

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. His-GST

    Plasmid

    #215169

    Purpose

    Encodes the synthetic glutathionine S-transferase with N-terminal His tag

    Depositor

    Travis Beddoe, Bhuvana Shanbhag

    Insert

    His-GST
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7 lac

    Available Since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLG032 (ppl)

    Plasmid

    #157910

    Purpose

    Expresses the ppl defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    ppl (PHP-ATPase)
    

    Expression

    Bacterial

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRsetB-his7-Perceval

    Plasmid

    #20336

    Depositor

    Gary Yellen

    Article

    Berg et al Nat Methods. 2009 Feb . 6(2):161-6.

    Insert

    Perceval (fluorescent ATP:ADP sensor)
    

    Tags

    his(7)

    Expression

    Bacterial

    Available Since

    March 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCR-Hyg

    Plasmid

    #158708

    Purpose

    For cloning and constitutive expression of crRNAs in mycobacterium

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

    Insert

    crRNA
    

    Use

    CRISPR and Synthetic Biology; Mycobacteria expres…

    Expression

    Bacterial

    Promoter

    Hsp60

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX-GST-His6-1_HBD

    Plasmid

    #171869

    Purpose

    Expresses a specific DNA-RNA hybrid sensor protein with the HBD domain of RNase H1 in bacterial cells

    Depositor

    Kaiwei Liang

    Article

    Wang et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabe

    Insert

    Hybrid binding domain of Rnase H1
    

    Tags

    GST- and His10-tag

    Expression

    Bacterial

    Mutation

    WT

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. p2CT-His-MBP-Lsh_C2c2_WT

    Plasmid

    #83487

    Purpose

    Bacterial expression of WT L. shahii C2c2 CRISPR effector

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

    Insert

    Lsh_C2c2_WT
    

    Use

    CRISPR

    Tags

    His6-MBP

    Expression

    Bacterial

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. MS2-Snapf

    Plasmid

    #65860

    Purpose

    Expresses RNA-binding protein MS2 with fast SNAP fusion tag

    Depositor

    Aaron Hoskins

    Article

    Carrocci et al Analyst. 2014 Jan 7;139(1):44-7. doi: 10

    Inserts

    MS2
    Fast SNAP
    

    Tags

    HA and NLS

    Expression

    Bacterial and Yeast

    Available Since

    Sept. 3, 2015

    Availability

    Academic Institutions and Nonprofits only