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pRS-45

Plasmid

#40586

Depositor

Harald Schwalbe

Article

Silvers et al Protein Expr Purif. 2012 Jul 24;85(1):14

Insert

yTAND12-TEV-hTBPc (TAF1, TBP Human, Budding Yeast)

Tags

His-tag and yTAND12

Expression

Bacterial

Promoter

T7

Available Since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only

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SW5

Plasmid

#40108

Depositor

Michel Maharbiz

Article

Huang et al J Biol Eng. 2012 Jul 9;6(1):9.

Insert

Bistable switch using dynamic degradation

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 12, 2012

Availability

Academic Institutions and Nonprofits only

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pETM41-EcPPXc

Plasmid

#38329

Depositor

Florian Freimoser

Article

Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

Insert

E. coli polyphosphatase (ppx Escherichia coli)

Tags

His, MBP, and TEV

Expression

Bacterial

Mutation

C-terminus of E. coli polyphosphatase (described …

Promoter

T7

Available Since

Aug. 6, 2012

Availability

Academic Institutions and Nonprofits only
pET mOCR His6 LIC cloning vector (2Oc-T)

Plasmid

#37238

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6 and mOCR

Expression

Bacterial

Promoter

T7

Available Since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pET22b-GFP-CPDSalI

Plasmid

#38257

Depositor

Matthew Bogyo, Aimee Shen

Article

Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

Insert

eGFP

Tags

6xHis and Vibrio cholerae MARTX toxin cysteine pr…

Expression

Bacterial

Mutation

cloned from pEGFPN3 (Clonetech)

Promoter

T7

Available Since

July 20, 2012

Availability

Academic Institutions and Nonprofits only
pLS1

Plasmid

#31490

Depositor

Kathleen Matthews

Article

Swint-Kruse et al Protein Sci. 2001 Feb . 10(2):262-76.

Insert

lactose repressor protein (lacI )

Expression

Bacterial

Available Since

June 21, 2012

Availability

Academic Institutions and Nonprofits only
pBbB7k-GFP

Plasmid

#35360

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pBbB6c-GFP

Plasmid

#35356

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

LlacO-1

Available Since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pBbA2a-RFP

Plasmid

#35325

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

Tet

Available Since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pBbE2a-RFP

Plasmid

#35322

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

Tet

Available Since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pBbE7k-RFP

Plasmid

#35315

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbS5a-RFP

Plasmid

#35283

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

lacUV5

Available Since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pBbA5a-RFP

Plasmid

#35280

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

lacUV5

Available Since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pBbA8c-RFP

Plasmid

#35272

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

BAD

Available Since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pUC57-codon-modified HTK

Plasmid

#34563

Depositor

David Anderson

Article

Lo et al Neuron. 2011 Dec 22;72(6):938-50.

Insert

codon-modified HTK

Expression

Bacterial

Mutation

codon modified HTK using mouse codon usage table

Promoter

pUC57 bacteria promoter

Available Since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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HRMT1L3

Plasmid

#25508

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

HRMT1L3 (PRMT3 Human)

Tags

His

Expression

Bacterial

Mutation

contains amino acids 149-463 only

Available Since

Oct. 24, 2011

Availability

Industry, Academic Institutions, and Nonprofits
pSC300

Plasmid

#31820

Depositor

Yossef Av-Gay

Article

Cowley et al Gene. 2001 Feb 21;264(2):225-31.

Insert

GFP (lacking ATG start codon)

Tags

GFP (lacking ATG start codon)

Expression

Bacterial

Promoter

SOD

Available Since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pJT106b3

Plasmid

#31393

Depositor

Christopher Voigt

Article

Tabor et al J Mol Biol. 2011 Jan 14. 405(2):315-24.

Insert

red light inverter with lacZ output

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only

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pET Biotin His6 FlAsH LIC cloning vector (H6-FlAsH)

Plasmid

#30184

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, AviTag (biotin), FlAsH, and TEV cleavage s…

Expression

Bacterial

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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pEN12A-P1

Plasmid

#27366

Depositor

Dirk Schnappinger

Article

Blumenthal et al PLoS One. 2010 Dec 22;5(12):e15667. doi:

Insert

Pmyc1tetO

Use

Gateway Cloning

Expression

Bacterial

Mutation

N/A

Available Since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

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