32,656 results
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Plasmid#138129PurposeExpresses GST-tagged mClover3-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.DepositorInsertmClover3-anti-rabbit-IgG(Fc)-nanobody
TagsGSTExpressionBacterialAvailable SinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKCyR8
Plasmid#149494PurposeBroad-Host range expression vector, RK2 and pUC origin, CymR(AM) repressor, PCymRC promoter, mRFP, Hygromycin Resistance Marker.DepositorInsertCymR(AM)-mRFP
UseSynthetic BiologyExpressionBacterialPromoterPCymRCAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQE81XN-mGold
Plasmid#218959PurposeExpresses Hisx6-Tagged mGold in bacteriaDepositorInsertmGold
Tags6X-HisExpressionBacterialPromoterT5Available SinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p1-hPdcd4
Plasmid#20693DepositorInsertProgrammed Cell Death Protein 4 (PDCD4 Human)
TagsGSTExpressionBacterialMutationI36V- does not alter functionAvailable SinceJune 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLG003 (Druantia type I)
Plasmid#157881PurposeExpresses the Druantia type I defense systemDepositorInsertDruantia type I
ExpressionBacterialAvailable SinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2TAL3-RR
Plasmid#37276PurposeTALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-term tal protein segments increases mutation induction; Fok I RR (improved version = pCS2TAL3-RRR)DepositorTypeEmpty backboneTagsC terminal Tal From pTAL3, FokI RR, HA, and N-ter…ExpressionBacterial and MammalianPromoterCMVAvailable SinceJune 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pNZ18-CAH
Plasmid#203159PurposeReplicates in E. coli, S. cerevisiae, and A. laidlawiiDepositorInsertYeast centromere, autonomously replicating sequence, histidine auxotrophic marker
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJJS001
Plasmid#188324PurposePCR template for repair templates to tag a gene with a GFP and mIAA7 AID degron using homology-directed repair for C. elegans.DepositorInsertmIAA7::GFP::Linker
ExpressionBacterialAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGR596
Plasmid#213787PurposeIntegrates at the CAN1 locus the BadBoy3 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassetteDepositorInsertBadBoy3 TP-DNAP1
UseSynthetic BiologyExpressionBacterial and YeastMutationSee manuscriptAvailable SinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEC3109 (pSpy1C-Plac(1)_ffluc)
Plasmid#218514Purposereplicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressorDepositorInsertffluc
UseLuciferaseExpressionBacterialMutationdeletion of mrfp cassette, mutagenesis of the RBS…PromoterPlac(1)Available SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBWB424
Plasmid#140638PurposeRSF1010-Cas9-vanAB gRNA. Vector with constitutive Cas9 and gRNA targeting vanAB locus.DepositorInsertCas9
ExpressionBacterialAvailable SinceMay 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 a-syn A53T D115A mNeonGreen-3K-B11
Plasmid#232017PurposeBacterial expression of alpha synuclein A53T mutant, tagged with the final beta strand of mNeonGreen-3KDepositorAvailable SinceAug. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-IQGAP1
Plasmid#30107DepositorAvailable SinceJuly 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pKnockinDonor-human-p16INK4A-P2A-mCherry
Plasmid#137026Purposedonor vector to knock in P2A-mCherry to the C-terminus of endogenous human p16INK4ADepositorInsertp16INK4A-knockin-homology arm-P2A-mCherry
TagsP2A-mCherryExpressionBacterialPromoterno promoterAvailable SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
2Bc-T MBP_BoMoC(ed)_I770A_6xH
Plasmid#185711PurposeRecombinant B. mori truncated R2 RT expressionDepositorInsertB. mori truncated R2 RT
Tags6xHis and MBPExpressionBacterialMutationChanged I770 to APromoterT7Available SinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28C-mCherry-MED1LCD
Plasmid#204404PurposeE. coli expression of MED1 low-complexity domain (947-1581) with an N-terminal mCherry tagDepositorAvailable SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HsSENP1
Plasmid#71465PurposeEncodes human full length SENP1 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).DepositorInsertSUMO1/sentrin specific peptidase 1 (SENP1 Human)
TagsHexahistidineExpressionBacterialMutationGene optimised for E. coli expression (Genscript)PromoterT7Available SinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLG024 (SIR2 + HerA)
Plasmid#157902PurposeExpresses the SIR2 + HerA defense systemDepositorInsertSIR2 + HerA
ExpressionBacterialAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDM4
Plasmid#49796PurposeePathBrick vector for pathway engineering in E. coli.DepositorTypeEmpty backboneTagsS-tagExpressionBacterialPromoterT7Available SinceJan. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGP-Tn7-PrpsM
Plasmid#225282PurposeMini-Tn7 pGP-Tn7-Cm vector with constitutive PrpsM promoter for the constitutive expression of target genes inserted into the attTn7 chromosomal site.DepositorTypeEmpty backboneExpressionBacterialPromoterConstitutive PrpsM promoterAvailable SinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only