30,806 results

1. pNP1 sfGFP toehold switch

   Plasmid

   #107355

   Purpose

   Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 sfGFP trigger is co-expressed

   Depositor

   James Collins

   Article

   Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

   Insert

   toehold 7 + sfGFP
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETM33_MDM2_SWIB

   Plasmid

   #178481

   Purpose

   Bacterial expression of human domain with His-tag and GST tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   MDM2_SWIB (MDM2 Human, Synthetic)
   

   Tags

   His-GST

   Expression

   Bacterial

   Promoter

   T7/LacO

   Available Since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLz37_CB_operon_CsoS2_N1-N4

   Plasmid

   #140856

   Purpose

   Halothiobacillus neapolitanus carboxysome operon with full length CsoS2. For heterologous expression of carboxysomes in E. coli.

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   CbbL, CbbS, CsoS2 (full-length), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
   

   Expression

   Bacterial

   Available Since

   Aug. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS2TAL3-DD

   Plasmid

   #37275

   Purpose

   TALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-terminal tal protein segments increases mutation induction; Fok I DD (improved version=pCS2TAL3-DDD)

   Depositor

   David Grunwald

   Article

   Dahlem et al PLoS Genet. 2012 Aug;8(8):e1002861. Epub

   Type

   Empty backbone

   Tags

   Flag, Fok I DD, Tal-C, and Tal-N

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   June 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJV53-Cas12a

   Plasmid

   #158706

   Purpose

   CRISPR system used for genome editing in Mycobacterium smegmatis, expresses codon-optimized FnCpf1 and the recombination proteins gp60 and gp61

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

   Insert

   FnCpf1
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   TetO

   Available Since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMRE-Tn5-135

   Plasmid

   #118521

   Purpose

   miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mScarlet-I
   

   Use

   Minitn5 delivery vector

   Expression

   Bacterial

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET30-ARH3-His-Sumo-HA

   Plasmid

   #111578

   Purpose

   Expresses ARH3 (amino acids 14-363) in E. coli

   Depositor

   Thomas Muir

   Article

   Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

   Insert

   ARH3 (ADPRS Human)
   

   Tags

   6xHis-Sumo and HA

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-13

   Promoter

   T7

   Available Since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFCiR5

   Plasmid

   #149479

   Purpose

   Broad-Host range expression vector, RSF1010 origin, CinR(AM) activator, PCin promoter, mRFP, Gentamicin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   CinR(AM)-mRFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PCin

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. PYCR1

   Plasmid

   #38842

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   PYCR1 (PYCR1 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pET28a-Ec.coaD (pESC106)

    Plasmid

    #50388

    Purpose

    Heterologous expression of E. coli CoA biosynthetic enzyme, PPAT (phosphopantetheine adenylyltransferase). Contain 6xHis-tag for nickel affinity purification.

    Depositor

    Tadhg Begley, Erick Strauss

    Article

    Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

    Insert

    PPAT
    

    Tags

    6xHis-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQE-BR1

    Plasmid

    #160333

    Purpose

    This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is improved compared to wild type.

    Depositor

    Sang Taek Jung

    Article

    Ko et al J Agric Food Chem. 2019 Oct 30;67(43):12

    Insert

    BR1
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOPINS-UBE3C

    Plasmid

    #66711

    Purpose

    Expresses UBE3C HECT domain in E. coli

    Depositor

    David Komander

    Article

    Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

    Insert

    KiAA0010 (UBE3C )
    

    Tags

    His-SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 17, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pJL1-dTomato

    Plasmid

    #102631

    Purpose

    In vitro expression of tdTomato from the T7 promoter

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

    Insert

    tdTomato
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28_mouse-pro-IL1B

    Plasmid

    #183386

    Purpose

    Overexpression of catalytic domain of inflammatory caspase in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    pro-IL1B (Il1b Mouse)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNH-TrxT-G-L11

    Plasmid

    #127831

    Purpose

    Production of recombinant human ribosomal protein L11

    Depositor

    Ivanhoe Leung

    Article

    Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

    Insert

    RPL11 (RPL11 Human)
    

    Tags

    His6 - Thioredoxin - TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCS2TAL3-RR

    Plasmid

    #37276

    Purpose

    TALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-term tal protein segments increases mutation induction; Fok I RR (improved version = pCS2TAL3-RRR)

    Depositor

    David Grunwald

    Article

    Dahlem et al PLoS Genet. 2012 Aug;8(8):e1002861. Epub

    Type

    Empty backbone

    Tags

    C terminal Tal From pTAL3, FokI RR, HA, and N-ter…

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    June 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGJJ001

    Plasmid

    #183619

    Purpose

    bindingPCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR under the expression of two CYC promoters.

    Depositor

    Ben Lehner

    Article

    Faure et al Nature. 2022 Apr;604(7904):175-183. doi:

    Type

    Empty backbone

    Expression

    Bacterial and Yeast

    Available Since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMBP-SpyCatcher

    Plasmid

    #79053

    Purpose

    Expresses maltose binding protein (MBP) SpyCatcher fusion

    Depositor

    James Bowie

    Article

    Min et al Protein Sci. 2016 Aug;25(8):1535-44. doi

    Insert

    MBP-SpyCatcher
    

    Tags

    6xHis and Maltose binding protein

    Expression

    Bacterial

    Mutation

    Derived from pDEST14-SpyCatcher (Plasmid #35044)…

    Promoter

    T7

    Available Since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC8 human IFN-gamma

    Plasmid

    #17600

    Depositor

    Howard Young

    Article

    Gonsky et al J Immunol. 2000 Feb 1. 164(3):1399-407.

    Insert

    interferon-gamma cDNA (IFNG Human)
    

    Expression

    Bacterial

    Available Since

    May 11, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pNR276

    Plasmid

    #79577

    Purpose

    TP901 attB-attP. GFP will express if sites recombine.

    Depositor

    Timothy Lu

    Article

    Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

    Insert

    GFPmut3b
    

    Expression

    Bacterial

    Available Since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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