29,937 results
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Plasmid#160464PurposeEncodes a fusion of human proinsulin and the monomeric enhanced EGFP. This construct can be used as a negative control for superfolder activity in bacteriaDepositorInsertproinsulin-mEGFP
UseTagsExpressionBacterialMutationPromoterT5Available sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSEVA221rAPOBEC1-T7RNAP
Plasmid#167979PurposeExpresses fusion protein rAPOBEC1-T7RNAP in E. coliDepositorInsertrAPOBEC1-T7RNAP (T7p07 Synthetic)
UseTagsT7 tag and T7RNAPExpressionBacterialMutationPromoterTetR-PtetAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRT498-OtUBD(V203D)
Plasmid#190090PurposeExpresses OtUBD(V203D) mutant that is unable to bind ubiquitin with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coliDepositorInsertOtUBD(V203D)
UseTags6xHis and MBPExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b-HRASLS3-1-132
Plasmid#100725PurposeExpression of N-terminal region of human HRASLS3 in E.coliDepositorInsertHRASLS3 (PLAAT3 Human)
UseTagsHisExpressionBacterialMutationPromoterT7 promotorAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046785
Plasmid#145684PurposeBacterial Expression plasmid for SARS-CoV-2 N (nucleocapsid)DepositorInsertSARS-CoV-2 N (nucleocapsid) (N Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;StrepIIExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC19 PKC delta
Plasmid#8427DepositorInsertMouse Protein Kinase C delta (Prkcd Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDT386
Plasmid#174733PurposeExpresses His-tagged DT386, a truncated diphtheria toxin without a receptor-binding domain.DepositorInsertDT386
UseTagsHistagExpressionBacterialMutationDeleted amino acids 412-560 (the receptor binding…PromoterT5Available sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-yibDp-GFPuv
Plasmid#127078PurposeLow phosphate inducible yibD gene promoter expressing GFPuv reporterDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromoteryibDpAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
peSCBE3-NG-Hypa
Plasmid#218165PurposeThis plasmid harbors the base editor eSCBE3-NG-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity cytosine base editing at targets bearing NG PAM in StreptomycesDepositorInsertsescbe3-NG-Hypa
a programmable sgRNA cloning cassette
UseCRISPR; Sgrna transcriptionTagsExpressionBacterialMutationMutation in the portion of deaminase hAPOBEC3A : …PromoterrpsL promoterAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc12-HiBiT
Plasmid#198308PurposeProduces PVCs tagged with HiBiT on the baseplate; used as assembly/loading control for payload loading assaysDepositorInsertPVCpnf_pvc12-HiBiT
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-mNectarine
Plasmid#21717PurposeBacterial expression of pH-sensitive monomeric mNectarine red fluorescent proteinDepositorInsertmNectarine fluorescent proteins
UseTagsHisExpressionBacterialMutationThe fluorescent protein was inserted between XhoI…PromoterAvailable sinceAug. 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
pH6-TEV-mCherry
Plasmid#132430PurposeExpresses His6-tagged mCherry in E. coli using T7 promoterDepositorInsertmCherry
UseTagsN-terminal His6 tag with TEV protease siteExpressionBacterialMutationCodon optimizedPromoterT7Available sinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
EV-D68 3C protease; aliases: Enterovirus A71 3C protease, non cleavable C-term 6xHis tag
Plasmid#204817PurposeBacterial expresison of Enterovirus D68 3C proteaseDepositorInsertEV D68 3C protease
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
Zika NS5 RdRp
Plasmid#204788PurposeBacterial expression of Zika NS5 RdRpDepositorInsertZika NS5 RdRp domain
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pETM6-HpaBC
Plasmid#71260PurposeExpresses E. coli HpaBC in monocistronic form from ePathBrick vector, pETM6DepositorInsertsHpaB
HpaC
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
RS35075_pMQ30_KO_Kan
Plasmid#185390PurposeNon-replicating vector used to create markerless deletion of RR42_RS35075 in C. basilensis 4G11DepositorInsertsRR42_RS35075 upstream flanking homology region
RR42_RS35075 downstream flanking homology region
nptII kanamycin resistance marker
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFS_0235-pET30-Fusicatenibacter_saccharivorans_ARRAY2_FaqI
Plasmid#116954PurposeExpresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD3 (OTU, aa 52-209)
Plasmid#61412PurposeExpresses human OTUD3 (OTU domain) in E. coli.DepositorInsertOTUD3 (OTUD3 Human)
UseTagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-51 and aa 210-398.PromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCK01_PalkS_alkS
Plasmid#166502PurposeExpression of AlkS transcriptional regulator with L401G mutation. Sensor input plasmid for detection of n-butanol and branched-chain higher alcohols.DepositorInsertalkS
UseSynthetic BiologyTagsExpressionBacterialMutationL401GPromoterPalkSAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAB_PalkB_sfgfp_KanR
Plasmid#166503PurposeExpression of sfGFP under control of PalkB promoter. Sensor output plasmid for detection of n-butanol and branched-chain higher alcohols.DepositorInsertsfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPalkBAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only