30,535 results

1. pGEX Sap-SH2

   Plasmid

   #46477

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Sap (SH2D1A Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 4-128

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHA2

   Plasmid

   #85710

   Purpose

   This plasmid provides a BAC vector backbone containing GFP, which can be used for cloning of BAC inserts, for instance, herpesvirus genomes

   Depositor

   Martin Messerle, Ulrich Koszinowski

   Article

   Adler et al J Virol. 2000 Aug;74(15):6964-74.

   Type

   Empty backbone

   Use

   Cre/Lox; Herpesvirus cloning

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET22b-PelB-6H-Ag43-160N-sfGFP

   Plasmid

   #172886

   Purpose

   Display sfGFP protein on E. coli cell surface

   Depositor

   Urartu Seker

   Article

   Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

   Inserts

   SfGFP
   Ag43, partial (flu )
   

   Use

   Tags

   HHHHHH and TEV recognition site (ENLYFQG)

   Expression

   Bacterial

   Mutation

   Deleted 1-160 (N terminal)

   Promoter

   T7 Promoter

   Available Since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pREDTKI

   Plasmid

   #51628

   Purpose

   E coli Genetic Modification

   Depositor

   Sheng Yang

   Article

   Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

   Insert

   I-SceI (SCEI Budding Yeast)
   

   Use

   Red recombinase and i-scei endonuclease

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   trc

   Available Since

   May 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMMLV-EGFP-P2A-Puro

   Plasmid

   #160226

   Purpose

   Expresses EGFP in mammalian cells

   Depositor

   Jason Sheltzer

   Article

   Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

   Insert

   EGFP-P2A-PuroR
   

   Use

   Retroviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   LTR

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a His6HuR

   Plasmid

   #135943

   Purpose

   Expresses HuR in E. coli

   Depositor

   Yimon Aye

   Article

   Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

   Insert

   ELAV like RNA binding protein 1 (ELAVL1 Human)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST14-DoubleCatcher

   Plasmid

   #216284

   Purpose

   Expresses SpyCatcher003-SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow stepwise coupling of two unique SpyTag-linked binders

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   DoubleCatcher
   

   Use

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNC01

   Plasmid

   #233643

   Purpose

   For bacterial expression of sfGFP c-terminally tagged with VitelloTagA.

   Depositor

   Zak Swartz

   Article

   Clarke et al Development. 2024 Oct 15;151(20):dev2028

   Insert

   sfGFP with c-terminal VitelloTagA
   

   Use

   Tags

   6-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET24a-VHH-mCherry

   Plasmid

   #109421

   Purpose

   Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCherry. VHH-mCherry also contains a T7, HA, BAP and His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

   Insert

   anti-GFP nanobody fused to a T7, mCherry, HA, BAP and His6 epitope
   

   Use

   Tags

   BAP, HA, His6, and T7

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBAD/His-B-iRFP

    Plasmid

    #31855

    Purpose

    a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging

    Depositor

    Vladislav Verkhusha

    Article

    Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

    Insert

    iRFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    S13L, A92T, V104I, V114I, E161K, Y193K, F198Y, D2…

    Promoter

    araBAD

    Available Since

    Sept. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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