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pAG247 His-iFAST

Plasmid

#130808

Purpose

Expresses His-tagged iFAST in bacteria

Depositor

Arnaud Gautier

Article

Tebo et al Biochemistry. 2018 Oct 2;57(39):5648-565

Insert

iFAST

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pC0057 BzoCas13 (B01) His6-TwinStrep-SUMO-BsaI

Plasmid

#115207

Purpose

Expresses BzoCas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

BzoCas13

Tags

SUMO

Expression

Bacterial

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pBad-UXPS

Plasmid

#172138

Purpose

bacteria expression plasmid for a UDP/UTP sensor

Depositor

Huiwang Ai

Article

Li et al ACS Cent Sci. 2021 Oct 27;7(10):1763-177

Insert

UXPS

Expression

Bacterial

Promoter

araBAD

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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xGoosecoid

Plasmid

#24977

Depositor

Edward De Robertis

Article

Cho et al Cell. 1991 Dec 20. 67(6):1111-20.

Insert

Goosecoid (gsc-a Frog)

Expression

Bacterial

Available Since

June 29, 2010

Availability

Academic Institutions and Nonprofits only
pET_RGG

Plasmid

#124929

Purpose

Expression of RGG domain from LAF-1 gene

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

LAF-1 RGG domain (laf-1 Nematode)

Tags

His

Expression

Bacterial

Mutation

RGG domain

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 3xFlag-CREB

Plasmid

#82721

Purpose

Expresses human CREB

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

CREB (CREB1 Human)

Tags

3XFLAG and GST

Expression

Bacterial

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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6920 pPROEXHsp104b

Plasmid

#1230

Depositor

Susan Lindquist

Article

Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

Insert

Hsp104 (HSP104 Budding Yeast)

Tags

his tag (tev cleavable)

Expression

Bacterial

Available Since

Aug. 15, 2005

Availability

Academic Institutions and Nonprofits only
pZE27MC3

Plasmid

#75464

Purpose

Plasmid for constitutive expression of mcherry-pdt3

Depositor

James Collins

Article

Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

Insert

mcherry-pdt#3

Expression

Bacterial

Promoter

placIq promoter

Available Since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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HLA-A2.1

Plasmid

#14904

Depositor

Linda Sherman

Article

Irwin et al J Exp Med. 1989 Oct 1. 170(4):1091-101.

Insert

HLA-A2.1 (HLA-A Human)

Expression

Bacterial

Available Since

June 15, 2007

Availability

Academic Institutions and Nonprofits only

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pHH0103_GAS7-1/1

Plasmid

#91301

Purpose

Protein expression and purification of human SH3 domain construct GAS7-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

GAS7-1/1 (GAS7 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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XP12 Xbra

Plasmid

#16978

Depositor

Randall Moon

Insert

Xbra (t.S Frog)

Expression

Bacterial

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pGEX Src-SH2

Plasmid

#46510

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Src (SRC Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 142-252

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pPGN-K

Plasmid

#174579

Purpose

pOPIN-GG expression vector compatible with N-terminal tagging, Kanamycin resistant

Depositor

Mark Banfield

Type

Empty backbone

Expression

Bacterial

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_VASP_WH1

Plasmid

#109790

Purpose

Protein expression and purification of VASP_WH1

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

VASP_WH1 (VASP Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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UbcH5B

Plasmid

#60443

Purpose

Express E2 UbcH5B in E. Coli

Depositor

Cynthia Wolberger

Article

Wiener et al Nat Struct Mol Biol. 2013 Sep;20(9):1033

Insert

UbcH5B (UBE2D2 Human)

Expression

Bacterial

Promoter

T7

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
pUC18T-mini-Tn7T

Plasmid

#64957

Purpose

mini-Tn7 base vector with transcriptional terminator and oriT

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Type

Empty backbone

Expression

Bacterial

Available Since

Aug. 14, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pJPC12-ΔM13-mCherry-PR/PRM-GFP

Plasmid

#80859

Purpose

Reporter plasmid (mCherry and GFP)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

mCherry
GFP

Expression

Bacterial

Promoter

Lambda Pr and Lambda Prm

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pET21b-P35

Plasmid

#11808

Depositor

Guy Salvesen

Article

Riedl et al Biochemistry. 2001 Nov 6. 40(44):13274-8

Insert

P35

Expression

Bacterial

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
pTriEx-RhoA FLARE.sc Biosensor T19N

Plasmid

#12152

Depositor

Klaus Hahn

Article

Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

Insert

RhoA Biosensor T19N (RHOA Human)

Tags

6xHis

Expression

Bacterial, Insect, and Mamm…

Mutation

RhoA T19N (dominant-negative mutation)*

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pETM6-T7-VHH-GFPminimizer

Plasmid

#202488

Purpose

Expresses the nanobody GFP minimizer with T7 induction

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody GFP minimizer

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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