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pJK693

Plasmid

#72382

Purpose

Produces Escherichia coli cysteine desulfurase IscS with Ala260>Thr mutation, N-terminal His6-tag (H6EcIscS-A260T)

Depositor

T. J. Kappock

Insert

cysteine desulfurase (tRNA sulfurtransferase), PLP-dependent (iscS Escherichia coli str. K-12 substr. MG1655)

Tags

His6

Expression

Bacterial

Mutation

Mutated alanine-260 to threonine

Promoter

T7

Available Since

March 4, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-6P1-hPXR-LBD

Plasmid

#67769

Purpose

Expression GST-hPXR-LBD in bacteria

Depositor

Sridhar Mani

Article

Li et al J Biol Chem. 2013 May 10;288(19):13655-6

Insert

human pregnane X receptor (PXR) ligand binding domain (NR1I2 Human)

Tags

GST

Expression

Bacterial

Mutation

Contains amino acids 107 to 434 of human PXR

Promoter

Tac

Available Since

July 22, 2015

Availability

Academic Institutions and Nonprofits only

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Venus-pBAD

Plasmid

#54859

Purpose

Localization: Protein Expression Vector , Excitation: 515, Emission: 528

Depositor

Michael Davidson, Atsushi Miyawaki

Article

Nagai et al Nat Biotechnol. 2002 Jan;20(1):87-90.

Type

Empty backbone

Tags

6xHis and Venus

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pQE60 R130A wgMDH

Plasmid

#204266

Purpose

Bacterial expression of R130A wgMDH with IPTG induction - Active site mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

R130A

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pDZ617 pKAN 12xPP7 V4

Plasmid

#72237

Purpose

Floxed Kanamycin (G418) selection maker; 3' tagging vector used to add 12 repetitive PP7 version 4 RNA stem-loops to gene of interest

Depositor

Daniel Zenklusen

Article

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

12xPP7 V4

Use

Cre/Lox

Expression

Bacterial

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pLZ12Km2-P23R:TA:fflucRT

Plasmid

#88901

Purpose

Toxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expression

Depositor

Thomas Proft

Article

Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(2

Insert

toxin-antitoxin + Red-shifted firefly luciferase

Expression

Bacterial

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pET-26b-NbVHH05-HA-His

Plasmid

#171567

Purpose

Bacterial expression of HA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-HA-His)

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

NbVHH05-HA-His (UBE2J1 Human)

Tags

HA-tag/His-tag and PelB signal peptide

Expression

Bacterial

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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AURKB

Plasmid

#39119

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

AURKB (AURKB Human)

Tags

His Tev

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
IL13 promoter luciferase

Plasmid

#11784

Depositor

Anjana Rao

Article

Macian et al EMBO J. 2000 Sep 1. 19(17):4783-95.

Insert

IL-13 promoter (IL13 Human)

Use

Luciferase

Tags

luciferase

Expression

Bacterial

Available Since

May 4, 2006

Availability

Academic Institutions and Nonprofits only
His-GIV-CTs

Plasmid

#69864

Purpose

Express His tagged GIV-CTs (1660–1870).

Depositor

Pradipta Ghosh

Article

Garcia-Marcos et al Mol Biol Cell. 2011 Mar 1;22(5):673-86.

Insert

GIV/Girdin (CCDC88A Human)

Tags

His

Expression

Bacterial

Mutation

C terminal amino acids S1660-S1870 of human GIV.

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pET-21a(+)/MBP-His-PINK1

Plasmid

#38005

Depositor

Michael J Fox Foundation MJFF

Insert

PINK1 (PINK1 Human)

Tags

6xHIS and MBP

Expression

Bacterial

Available Since

March 10, 2014

Availability

Academic Institutions and Nonprofits only
NSUN5

Plasmid

#25491

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

NSUN5 (NSUN5 Human)

Tags

His

Expression

Bacterial

Available Since

Aug. 23, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pGEX-6P-acatenin

Plasmid

#186458

Purpose

Expresses human alpha-catenin with an N-terminal GST tag and a PRescission Protease cleavage site

Depositor

Tina Izard

Article

Rangarajan et al Commun Biol. 2023 Mar 16;6(1):276. doi:

Insert

CTNNA1 (CTNNA1 Human)

Tags

GST

Expression

Bacterial

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pMMB67EH-Spy

Plasmid

#90102

Purpose

IPTG-inducible CFP/YFP FRET-based biosensor for cyclic-di-GMP measurement using YcgR binding site.

Depositor

Samuel Miller

Article

Christen et al Science. 2010 Jun 4;328(5983):1295-7. do

Insert

YcgR (ycgR )

Use

Broad-host range

Expression

Bacterial

Promoter

Ptac

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pIA1414

Plasmid

#172402

Purpose

Expression of SARS-COV-2 Nsp9 in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

Insert

SARS-COV-2 Nsp9 (ORF1ab Synthetic)

Tags

His-SUMO

Expression

Bacterial

Promoter

T7

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pTPH418b

Plasmid

#185725

Purpose

Directed evolution of Cas9 adenine base editors with altered PAM compatibility

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

gIII(N)-NpuN-NpuC-gIII(C)

Use

CRISPR

Expression

Bacterial

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pBS_flGAD

Plasmid

#87031

Purpose

Expresses GAD (Glutamic Acid Decarboxylase) in Bacteria

Depositor

Janice Blum

Insert

flGAD (GAD2 Human)

Expression

Bacterial

Promoter

Lac-Z

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pDonor-GDH

Plasmid

#140629

Purpose

Encodes a mini-transposon derived from V. cholerae Tn6677 and T7-GDH cargo gene.

Depositor

Sheng Yang

Article

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

Mini-Tn6677, T7lac-GDH gene cassette

Use

CRISPR; Transposon

Expression

Bacterial

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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RAP1B

Plasmid

#55665

Purpose

expression of mouse RAP1B

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RAP1B (Rap1b Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
VN1263 pB2Hws_v28_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP1_Ddcm_WeaKRBS_TIR51_KanR

Plasmid

#235115

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP1.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Inserts

Asf1B (ASF1B Human)
IP1

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P-rpoA

Expression

Bacterial

Promoter

LtetO and lpp+lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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