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Plasmid#15734DepositorInsertTIF1 gamma (TRIM33 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEN-dCas9-CDA1-UGI
Plasmid#196248PurposePlasmid containing promoter of RecA from Xanthomonas oryzae driving expression of dead Cas9, cytosine deaminase CDA1, and glycosylase inhibitorDepositorInsertattL4-RecApro-dCas9-CDA1-UGI-attR1
UseTagsExpressionBacterialMutationPromoterRecA promoterAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-hygMV
Plasmid#73891Purposefission yeast drug selection markerDepositorInserthygMV
UseTagsExpressionBacterial and YeastMutationPromoterpombe TEFAvailable sinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HIS-TagGFP2-TEV-GGG-ELP(120nm)-Cys
Plasmid#90473PurposeElastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Term TagGFP2, and a TEV-cleavage site, leaving an N-terminal Sortase Recognition Sequence GGG and a C-terminal CysteineDepositorInsertTagGFP2-TEV-GGG-ELP(120nm)-Cys
UseTagsCysteine and Sortase-TagExpressionBacterialMutationPromoterT7Available sinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-AcKRS-CloDF
Plasmid#127415PurposeMutant of mmPylRS designed for incorporation of non-canonical amino acids (acyl-lysine derivatives) in to M13 bacteriophageDepositorInsertPyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
UseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJKR-H-acuR
Plasmid#62567PurposeAcrylate biosensor. High copy plasmid that expresses GFP proportional to acrylate concentration.DepositorInsertGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-PesaRlux
Plasmid#47802PurposePesaR promoter, luxCDABE, and terminator in pET17b (AmpR)DepositorInsertPesaR-luxCDABE-term
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPesaRAvailable sinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDAI-SceI-SacB
Plasmid#113635PurposeBroad host range replicative plasmid expressing the I-SceI homing endonuclease and the counterselectable marker SacBDepositorInsertsacB
UseTagsExpressionBacterialMutationPlease see Depositor CommentsPromotersacB promoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMG046 pETARA-CK1α-His
Plasmid#154074PurposeExpresses GST_CK1α (human CK1α as a fusion protein with GST and His- tags) in E.coliDepositorInsertCSNK1A1 (CSNK1A1 Human)
UseTagsGST and His6ExpressionBacterialMutationPromoterT7Available sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOPINVHH_BAP_his
Plasmid#210402PurposeFor expression of VHH with C-terminal biotin acceptor peptide and his tagDepositorTypeEmpty backboneUseTagsbiotin acceptor peptide-his6ExpressionBacterialMutationPromoterlacAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINVHH_his
Plasmid#210405PurposeFor expression of VHH with C-terminal his tagDepositorTypeEmpty backboneUseTagshis6ExpressionBacterialMutationPromoterlacAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET16b-Trx CC32/35SS
Plasmid#36946DepositorInsertThioredoxin 1 (TXN Human)
UseTagsHis tagExpressionBacterialMutationboth active site cysteines (32,35) mutated to ser…PromoterT7Available sinceMay 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCri-18a
Plasmid#61326PurposeProtein expression.DepositorInsertGreen fluorescent protein
UseTagsHis-tagExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
AURKA_HUMAN_D0
Plasmid#79739PurposeThis plasmid encodes the kinase domain of AURKA. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertAURKA (AURKA Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Matr3 delRRM1
Plasmid#32881DepositorInsertMATR3 Matrin3 (MATR3 Human)
UseTagsFLAGExpressionBacterial and MammalianMutationdeleted amino acids 399-468PromoterCMVAvailable sinceJan. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PTBP1-RRM34
Plasmid#89156PurposeRecombinant expression of PTBP1-RRM34 in E.coliDepositorInsertPolypyrimidine Tract Binding Protein 1 RNA Recognition Motif 3+4 (PTBP1 Human)
UseTagshexa HisExpressionBacterialMutationcontains RNA Recognition Motif 3+4PromoterT7Available sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCM433kanT
Plasmid#61262PurposeSucrose counterselection (sacB) vector for unmarked allelic exchange.DepositorTypeEmpty backboneUseSynthetic Biology; Sucrose counterselectionTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUCXMG
Plasmid#204409PurposeExpression plasmid for cloning of FatI digested metagenomic DNA into a compatible NcoI siteDepositorTypeEmpty backboneUseTagsHis6ExpressionBacterialMutationPromotertacAvailable sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCON1-ProB.mCherry
Plasmid#112520PurposeProB driven mCherry expression with synTTDepositorInsertProB mCherry TT
UseTagsExpressionBacterialMutationPromoterProBAvailable sinceJuly 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAD-KPS12-Pman
Plasmid#133832PurposeFluorescent protein mKate(KPS12) variant for optimized expression in Bacillus mycoides; under control of mannose inducible promoter.DepositorInsertmKate(KPS12)
UseE.coli/bacillus spp. shuttle vectorTagsExpressionBacterialMutationPromoterPmanAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only