32,167 results
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Plasmid#174673PurposeExpresses 6xHis-MBP tagged BoCas12aDepositorArticleInserthuBoCas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV and NLS, 6xHis, 3xHAExpressionBacterialPromoterT7Available SinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046955
Plasmid#145616PurposeBacterial Expression plasmid for RNA-dependent RNA polymeraseDepositorInsertRNA-dependent RNA polymerase (Nsp12) (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;FLAG_2ExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
Rac1
Plasmid#55692PurposeExpression of mouse Rac1DepositorAvailable SinceSept. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-hKdm2b-CxxC-PHD
Plasmid#49216PurposeBacterial expression of human Kdm2b CxxC-PHD domain with N-His6-TEV and C-Avi tags. For use in BioCAP non-methylated DNA purification.DepositorAvailable SinceDec. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFG036
Plasmid#137996PurposecI repressor expression plasmidDepositorInsertcI
UseSynthetic BiologyExpressionBacterialMutationThermosensitiveAvailable SinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbB8k-csg-nb112
Plasmid#171702PurposeArabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a nanobody targeting the spike protein of SARS-CoV-2 via a flexible linker.DepositorInsertcsgBACEFG, with nanobody fused to CsgA
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMetTU1-A_Sv5K_araC-PBAD_MCD1_AnaA-T6A-I48V_AnaCNJKFGW_Bba-B0015
Plasmid#202023PurposeExpresses Anabaena flos-aquae ARG cluster (GvpA T6A-I48V mutant) in E. coliDepositorInsertAnabaena flos-aquae ARG cluster (GvpA T6A-I48V mutant)
ExpressionBacterialMutationGvpA T6A-I48VAvailable SinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET His6 Mocr TEV LIC cloning vector (2O-T)
Plasmid#29710DepositorTypeEmpty backboneTagsHis6-Mocr-TEVExpressionBacterialAvailable SinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET23a-His-hRanGAP1tail
Plasmid#53139PurposeE.coli expression of Cterminal domain of human RanGAP1 with Nterminal His-tagDepositorInsertRanGAP1 (RANGAP1 Human)
TagsHisExpressionBacterialMutationC-terminal fragment (amino acids 398-587)PromoterT7Available SinceMay 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCD565
Plasmid#153027PurposedxCas9(3.7), MCP-SoxS(R93A/S101A), J306 scRNADepositorInsertdxCas9(3.7), MCP-SoxS(R93A/S101A), J306 scRNA
UseCRISPRExpressionBacterialMutationSoxS has R93A and S101A mutationsAvailable SinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKEW212-MBP-TEV-AcrVA4
Plasmid#115658PurposeExpresses AcrVA4 in bacteria via T7 RNAP, for purificationDepositorInsertAcrVA4
TagsMBP-TEVExpressionBacterialPromoterT7 RNAPAvailable SinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET14b_tev_PSG
Plasmid#59731PurposeExpresses PDZ3-SH3-GUK fragment of human ZO-1 in bacteriaDepositorAvailable SinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-Bst-LF-Idaho
Plasmid#170469PurposeExpresses His-tagged Bst-LF-Idaho in E. coliDepositorInsertBst-LF-Idaho
TagsN-terminal His-tagExpressionBacterialAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pET28a-RBP-LOV2
Plasmid#185792PurposeEncodes Light-oxygen-voltage-sensing domain 2 linked to RNA binding peptide (on N terminal end) via GS-linkerDepositorInsertRBP-LOV2
Tags10xHis, TEV protease cleavage site after His TagExpressionBacterialPromoterT7Available SinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMRE141
Plasmid#118493Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmTurquoise2
ExpressionBacterialAvailable SinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b T7pCONS sfGFP
Plasmid#154467PurposepET15b with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFPDepositorInsertsuper folder GFP
TagsHis-thrombinExpressionBacterialPromoterT7Available SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-NtC60αESNtLC60β
Plasmid#160878PurposeExpression of N. tabacum Rubisco large subunit (rbcL) and Rubisco chaperone protiens cpn60α, cpn60β, and E. coli groES in E. coli.DepositorInsertrbcL, cpn60α, cpn60β, groES
ExpressionBacterialPromoterrbcL: T7, chaperones: T7Available SinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-NtLNtC60αESC60β
Plasmid#160875PurposeExpression of N. tabacum Rubisco large subunit (rbcL), Rubisco chaperone protiens cpn60α, cpn60β, and E. coli groES in E. coliDepositorInsertrbcL, cpn60α, cpn60β, groES
ExpressionBacterialPromoterrbcL: pBAD, chaperones: T7Available SinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
COVID-SARS2 NSP16/10
Plasmid#159615PurposeBacterial co-expression vector fo Covid-SARS2 NSP16 and NSP10DepositorInsertsCovid-SARS2 Nsp16
Covid-SARS2 Nsp10
TagsHis6-Zb-TEV and noneExpressionBacterialMutationCodon-optimized for E. coli expressionPromoter(Bicistronic) and T7 - LacOAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
DDX3XA
Plasmid#42362PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pIB190
Plasmid#90197PurposeE. coli - Streptococci shuttle plasmid for gene expression in streptococci with P23 promoterDepositorTypeEmpty backboneTagsHis-tag and Strep-tagExpressionBacterialAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETDUET-GST AO7REwt
Plasmid#213935PurposeGST tagged AO7 encoding aa 126-258 for expression in bacteriaDepositorInsertAO7 aa 126-258 (RNF25 Human)
TagsGSTExpressionBacterialMutationAO7 cloned into the first multiple cloning site o…PromoterT7 promoter-1Available SinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-SUMO-hDDI2-D252N_pET11a
Plasmid#169122Purposeexpression of proteinDepositorAvailable SinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mCherryGGBsMTS
Plasmid#133625PurposeExpression vector for mCherry fused to a membrane targeting sequence (MTS) of the B.subtilis MinD (C-terminal fusion) and an N-terminal His-tag for purification.DepositorInsertmCherryGGBsMTS
ExpressionBacterialAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEB2_mScarletI_mVenusNB-MrkHR113A
Plasmid#182292Purposeexpresses c-di-GMP mutant biosensor in E. coliDepositorInsertmVenusNB-MrkHR113A
TagsmVenusNB internal fusion to MrkH with a R113A poi…ExpressionBacterialMutationchanged arginine 113 to alanine, MrkH numbering …PromoterproCAvailable SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
B1-4
Plasmid#111466Purposetranslational elongation factor TuDepositorInserttranslational elongation factor Tu
Tags6xHisExpressionBacterialAvailable SinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHS0766 pET45b(+) His14-MBP-TEV-AloTnpB-2 3' extension
Plasmid#176542PurposeBacterial expression of His14-MBP-TEV-AloTnpB-2 with 3' extension derived from locusDepositorInsertsMBP tag
TnpB
Tags3' extension derived from endogenous locus a…ExpressionBacterialAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS hBAF57
Plasmid#17877DepositorInsertBAF57 (SMARCE1 Human)
ExpressionBacterialAvailable SinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pHN1_lacUV5-mNG-PDT
Plasmid#165583PurposeExpresses mNeonGreen and C-terminally fused PDTDepositorInsertmNeonGreen-PDT
TagsProtein degradation tagExpressionBacterialPromoterPtrcAvailable SinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only