32,826 results
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Plasmid#170204PurposeE. coli expression of SARS-CoV-2 nucleocapsid proteinDepositorAvailable SinceMay 11, 2022AvailabilityAcademic Institutions and Nonprofits only
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pET28_mouse-pro-IL1B
Plasmid#183386PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
His6-EGFP-PH-PLCD1
Plasmid#183675PurposePtdIns(4,5)P2 biosensor for purification from bacteriaDepositorInsertPLCD1 (PLCD1 Human)
TagsHis6-EGFPExpressionBacterialMutationAmino acids 11-140PromoterT7Available SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-FRT
Plasmid#183241Purposeanhydrotetracycline (aTC) inducible sgRNA that targets FRT scar sites and arabinose inducible lambda RedDepositorInsertsgRNA-FRT
ExpressionBacterialPromoterP-tetAvailable SinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBR1-kan-Ptet-plu-gamma-ET_rhi145
Plasmid#180427PurposeGenome engineering of AgrobacteriumDepositorInsertplu-gamma-ET_rhi145
ExpressionBacterialAvailable SinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
H11-CAG-loxP-FNF-bpA-3pA-loxP-tdTomato
Plasmid#180156PurposetdTomato cre reporter with Hipp11 recombineering homology armsDepositorInserttdTomato
UseCre/Lox and Mouse TargetingExpressionBacterial and MammalianAvailable SinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-AcKRS-CloDF
Plasmid#127415PurposeMutant of mmPylRS designed for incorporation of non-canonical amino acids (acyl-lysine derivatives) in to M13 bacteriophageDepositorInsertPyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
ExpressionBacterialPromoteraraBADAvailable SinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDonor14-ShoHELIX-KanR (BO4)
Plasmid#181795PurposepDonor for ShoHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargoDepositorInsertKanR, R6K origin of replication
ExpressionBacterialAvailable SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
bactin:GFP-LAP2b
Plasmid#105980Purposetransgenesis, ubiquitous expressionDepositorInsertLAP2b
TagsGFPExpressionBacterialAvailable SinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b-CoilR-Cys
Plasmid#181989PurposeUsed to label proteins with the CoilE genetically encoded tagDepositorInsertCoilR-Cys
TagsHis-TagExpressionBacterialAvailable SinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b-miniR-DBCO
Plasmid#181984PurposeUsed to label proteins with the MiniE genetically encoded tagDepositorInsertMiniR-DBCO
TagsDBCO-Tag, His-TagExpressionBacterialAvailable SinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM33_SUFU_Sufu
Plasmid#178488PurposeBacterial expression of human domain with His-tag and GST tagDepositorAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM33_PEX14_Pex14
Plasmid#178484PurposeBacterial expression of human domain with His-tag and GST tagDepositorAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM33_MDM2_SWIB
Plasmid#178481PurposeBacterial expression of human domain with His-tag and GST tagDepositorAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM33_CLTC_Clathrin propeller
Plasmid#178471PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertCLTC_Clathrin propeller (CLTC Synthetic, Human)
TagsHis-GSTExpressionBacterialPromoterT7/LacOAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a Cdk2ap1CAN
Plasmid#178032PurposeExpression vector for the purpose of protein purification.DepositorInsertCdk2ap1 (Cdk2ap1 Mouse)
UseNonviralTags6xHIS - Thrombin - MBP- TEV-TRSExpressionBacterialPromoterT7LacIAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSL-1
Plasmid#180422PurposeKan resistant plasmid derived from pBTK402, a broad-host-range plasmid previously shown to replicate in multiple species of bee gut bacteria. It encodes a low level RFP promoter.DepositorInsertRed Chromprotein gene
ExpressionBacterialAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACYC-T7-Nla-repeat-BbsI
Plasmid#180215PurposeFor cloning Neisseria lactamica Type I-C crRNA for protein purification in E.ColiDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterT7Available SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCB
Plasmid#162699PurposeExpresses pHnCB10 derived carboxysome operon and prkDepositorInsertpHnCB10 derived carboxysome operon, prk
ExpressionBacterialPromoterPLtet0-1 promoterAvailable SinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
R26-CAG-loxP-FNF-bpA-3pA-loxP-tdTomato
Plasmid#180152PurposetdTomato cre reporter with Rosa26 recombineering homology armsDepositorInserttdTomato
UseCre/Lox and Mouse TargetingExpressionBacterial and MammalianAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pShHELIX(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT112)
Plasmid#181791PurposeExpresses 3-component ShHELIX containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertnAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
ExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
PRDX1A-c001
Plasmid#175473PurposeProtein expression in bacterial cells. Entire CDS, M1-K199. Can be biotinylated.DepositorAvailable SinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pATZ-HA-GFP11
Plasmid#177720PurposeER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to an HA tag and the eleventh β-strand of GFPDepositorInsertalpha-1-antitrypsin (SERPINA1 Human)
TagsGFP11 and HAExpressionBacterial and MammalianMutationE342K (ATZ mutant)Available SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
SDCBPA-c000
Plasmid#175480PurposeProtein expression in bacterial cells. Entire CDS, M1-V288.DepositorAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
YSCD44A-c002
Plasmid#175493PurposeProtein expression in bacterial cells. Single chain Fv antibody to human CD44 Link domain. Can be used for crystallography.DepositorInsertSingle chain Fv CD44 antibody (H90)
Tags3XFLAG-EK-His8 and pelBExpressionBacterialAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEcCas6
Plasmid#170091Purposeexpresses E. coli type I-E Cas6DepositorInsertE. coli type I-E Cas6
TagsHisExpressionBacterialPromoterT7Available SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
phFluc-WT
Plasmid#177714PurposeCytosolic firefly luciferase reporter previously described in Shcherbakov et al. 2019DepositorInserthFluc WT
ExpressionBacterial and MammalianAvailable SinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
ALC1-c005
Plasmid#173092PurposeExpression of recombinant fusion protein in E. coliDepositorAvailable SinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
MacroH2A.2-c002
Plasmid#173089PurposeExpression of recombinant fusion protein in E. coliDepositorAvailable SinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
Affibody HER2:243.BirA*-pET301/NT-DEST
Plasmid#174692PurposePlasmid encoding for the bacterial expression of a bispecific coding for the anti-HER2 affibody ZHER2:342 fused to the mutated form of BirA enzyme, BirA*DepositorInsertAnti-HER2 affibody ZHER2:342 fused to mutated form of BirA, BirA*
TagsHIS tagExpressionBacterialMutationR118G mutation in BirAPromoterT7 promoterAvailable SinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only