30,808 results

1. pCgVchcrRNA

   Plasmid

   #203815

   Purpose

   Expression of CRISPR RNA, shuttle vector for Corynebacterium glutamicum / E. coli

   Depositor

   Sheng Yang

   Article

   Yang et al ACS Synth Biol. 2023 Jul 21;12(7):2198-2

   Insert

   CRISPR(BsaI)
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKB256

   Plasmid

   #170039

   Purpose

   Encodes LacI-controlled expression of surface-displayed Bac2a and PvirK-mNeonGreen reporter

   Depositor

   Jeffrey Tabor

   Article

   Brink et al bioRxiv

   Inserts

   mNeonGreen
   Bac2a
   

   Use

   Synthetic Biology

   Tags

   Lpp, OmpA, and Tether

   Expression

   Bacterial

   Promoter

   Ptac and PvirK

   Available Since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-mH6-Cas12g1

   Plasmid

   #120879

   Purpose

   Expresses E. coli codon optimized Cas12g1 effector in the pET28a backbone.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

   Insert

   Cas12g1
   

   Use

   CRISPR

   Tags

   mH6

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJL1-mCherry

   Plasmid

   #102629

   Purpose

   In vitro expression of mCherry from the T7 promoter

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

   Insert

   mCherry
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMyCA

   Plasmid

   #84268

   Purpose

   A versatile vector for recombinant mycobacterial protein production

   Depositor

   Young-Hwa Song

   Article

   Magana Vergara et al Protein Sci. 2017 Aug 31. doi: 10.1002/p

   Type

   Empty backbone

   Tags

   His6

   Expression

   Bacterial

   Promoter

   Aceteamidase

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET11d-hUba2

   Plasmid

   #53136

   Purpose

   E.coli expression of full length untagged human Uba2 (SAE2)

   Depositor

   Frauke Melchior

   Article

   Pichler et al Cell. 2002 Jan 11;108(1):109-20.

   Insert

   UBA2 (UBA2 Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. TS245_pNKI-2-13_EGFP-DPN7

   Plasmid

   #200719

   Purpose

   producing Methyl Tracer EGFP protein in bacteria

   Depositor

   Bas van Steensel

   Article

   van Schaik et al Methods Mol Biol. 2022;2458:215-229. doi

   Insert

   eGFP-DPN7
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   May 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. tUI-HA

   Plasmid

   #122662

   Purpose

   Express tUI-HA protein in E. coli

   Depositor

   Robert Cohen

   Article

   Choi et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

   Insert

   tUI-HA
   

   Tags

   HA-tag and His-tag

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   July 17, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pET11a-AldoA

   Plasmid

   #224925

   Purpose

   Inducible expression of human aldolase A

   Depositor

   Liskin Swint-Kruse

   Article

   Fenton et al Protein Sci. 2022 Feb;31(2):357-370. doi

   Insert

   Aldolase A (ALDOA Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMRE-Tn5-155

    Plasmid

    #118537

    Purpose

    miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mScarlet-I
    

    Use

    Minitn5 delivery vector

    Expression

    Bacterial

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28-5'TwStrep

    Plasmid

    #208620

    Purpose

    Empty vector for prokaryotic expression, insert a Twin Strep tag between NdeI and BamHI site of pET28a (+), both site retained, T7 tag replaced, BamHI site in-frame with Twin Strep tag.

    Depositor

    Quanfu Ma

    Type

    Empty backbone

    Tags

    Twin Strep

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCS-PesaRlux

    Plasmid

    #47640

    Purpose

    wt esaR promoter in pCS26

    Depositor

    Cynthia Collins

    Article

    Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

    Insert

    PesaR
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PesaR

    Available Since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAWP78-PVCpnf_pvc12-HiBiT

    Plasmid

    #198308

    Purpose

    Produces PVCs tagged with HiBiT on the baseplate; used as assembly/loading control for payload loading assays

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf_pvc12-HiBiT
    

    Expression

    Bacterial

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSFDuet-1_hGH

    Plasmid

    #182410

    Purpose

    Bacterial expression of human growth hormone

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    hGH (GH1 Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28_6xHis-PARP1-CATdeltaHD

    Plasmid

    #174811

    Purpose

    Bacterial expression of a hyperactive PARP1 CAT lacking the autoinhibitory HD subdomain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-CATdeltaHD (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Residues 678-787 replaced by eight-residue linker…

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pVSL10

    Plasmid

    #201182

    Purpose

    Bst polymerase large fragment (BF) F710Y/D598A, His6-tagged, HRV 3C-cleavable

    Depositor

    Jack Szostak

    Article

    Lelyveld et al Science. 2023 Oct 27;382(6669):423-429.

    Insert

    Bst polymerase large fragment (BF) F710Y/D598A
    

    Tags

    HRV 3C protease cleavage site and His6

    Expression

    Bacterial

    Mutation

    F710Y D598A

    Promoter

    T5-lacO

    Available Since

    Sept. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA.3.1.ADRB2-NP

    Plasmid

    #134376

    Purpose

    Nanoluc complementation assay. Expression of adrenoceptor beta 2 fused at C termimus with Natural peptide (NP) of NanoLuc. Addition of signal sequence and Flag epitope at N terminus of ADRB2.

    Depositor

    Julien Hanson

    Article

    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

    Insert

    ADRB2-NP (ADRB2 Human)
    

    Tags

    Flag, natural peptide of nanoluciferase, and sign…

    Expression

    Bacterial and Mammalian

    Promoter

    T7

    Available Since

    Aug. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. HS-aPKC-CAAX-mKate2

    Plasmid

    #105950

    Purpose

    heat shock inducible transgenesis

    Depositor

    Caren Norden

    Article

    Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

    Insert

    aPKC-CAAX
    

    Tags

    mKate2

    Expression

    Bacterial

    Available Since

    Feb. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. p15aC-GFP

    Plasmid

    #107862

    Purpose

    Control plasmid expressing GFP

    Depositor

    Sanford Simon

    Article

    Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

    Insert

    GFP
    

    Tags

    myc

    Expression

    Bacterial

    Promoter

    proD

    Available Since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX-Rac1

    Plasmid

    #12200

    Depositor

    Jonathan Chernoff

    Article

    Bagrodia et al J Biol Chem. 1995 Sep 29. 270(39):22731-

    Insert

    Rac1 (RAC1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Starting methionine (M1) removed; F78S compared t…

    Available Since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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