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SpyCatcher002-oHept

Plasmid

#124671

Purpose

Expresses SpyCatcher002-oHept for heptamerization of SpyTag-proteins via heptameric coiled-coil

Depositor

Mark Howarth

Article

Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

Insert

SpyCatcher002-oHept

Tags

C-tag, His6, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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SSB-wt

Plasmid

#197260

Purpose

wild type E. coli SSB in pET21 expresses in BL21 under T7 control with lactose-amp resistance

Depositor

Timothy Lohman

Article

Antony et al J Mol Biol. 2013 Nov 29;425(23):4802-19.

Insert

wt E. coli SSB

Tags

none

Expression

Bacterial

Promoter

T7

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pET22b-msfGFP-NPM1

Plasmid

#237670

Purpose

For expression of msfGFP-NPM1 in Ecoli

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NPM1 (NPM1 Human)

Tags

msfGFP

Expression

Bacterial

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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V72 ELP

Plasmid

#68938

Purpose

Elastin-like Polypeptide (VPGxG) with 72 repeats containing Valine guest residue

Depositor

Andrew MacKay

Article

Dhandhukia et al Biomacromolecules. 2013 Apr 8;14(4):976-

Insert

V72 ELP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pHybrid-Electra2

Plasmid

#179475

Purpose

Expresses Electra2 in bacterial cells and Electra2 and mScarlet in mammalian cells

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Inserts

Electra2
mScarlet-I

Expression

Bacterial and Mammalian

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUD4 (OTU, aa 22-196)

Plasmid

#61413

Purpose

Expresses human OTUD4 (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD4 (OTU domain-containing protein 4) (OTUD4 Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-21 and aa 197-1114.

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pPSU100

Plasmid

#173835

Purpose

Expresses 100 kD Penn State ladder protein

Depositor

Song Tan

Article

Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

Insert

STRHSTPABPACIL1bQRS

Expression

Bacterial

Promoter

T7

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pBT13-splitC

Plasmid

#122596

Purpose

clone M13 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

M13 bacteriophage genes

Expression

Bacterial

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pBAD-jRCaMP1a

Plasmid

#113936

Purpose

Bacterial expression of red fluorescent Ca2+ sensor jRCaMP1a

Depositor

Robert Campbell, Thomas Hughes

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

jRCaMP1a

Tags

6xHis

Expression

Bacterial

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCV019-catP

Plasmid

#232825

Purpose

Random transposon mutagenesis vector for Bacteroidales species - chloramphenicol

Depositor

Athanasios Typas

Insert

catP

Expression

Bacterial

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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