30,808 results

1. pUG-natNT2

   Plasmid

   #110922

   Purpose

   expression of nat gene conferring resistance to nourseothricin for gene deletion in yeast

   Depositor

   Jean-Marc Daran

   Article

   Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

   Insert

   nat
   

   Expression

   Bacterial and Yeast

   Promoter

   AgTEF1

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEXndoS

   Plasmid

   #44655

   Depositor

   Mattias Collin

   Article

   Collin et al Infect Immun. 2001 Nov . 69(11):7187-9.

   Insert

   ndoS (ndoS Streptococcus pyogenes)
   

   Tags

   Gst

   Expression

   Bacterial

   Mutation

   None

   Promoter

   tac

   Available Since

   May 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-CohIII-CBM-HIS-ybbR

   Plasmid

   #60866

   Purpose

   Characteristic fingerprint molecule fused to cohesin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced ybbR tag

   Depositor

   Hermann Gaub

   Article

   Schoeler et al Nat Commun. 2014 Dec 8;5:5635. doi: 10.1

   Inserts

   CohIII
   CBM
   

   Tags

   6xHIS, HRV 3C, and ybbR

   Expression

   Bacterial

   Mutation

   Cysteine 63 to Serine in CBM module

   Promoter

   T7

   Available Since

   Nov. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAJM.884

   Plasmid

   #108536

   Purpose

   AcuRAM + PAcu-YFP Reporter. Acr sensor for use in non-Marionette strain.

   Depositor

   Christopher Voigt

   Article

   Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

   Insert

   AcuRAM + PAcu-YFP
   

   Expression

   Bacterial

   Mutation

   AcuR: E80K R154H L202M

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. TRAV16

   Plasmid

   #123389

   Purpose

   construction of TCR

   Depositor

   Catherine Wu

   Article

   Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

   Insert

   TRAV16 (TRAV16 Human)
   

   Expression

   Bacterial

   Mutation

   WT

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. TRAV13-1

   Plasmid

   #123386

   Purpose

   construction of TCR

   Depositor

   Catherine Wu

   Article

   Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

   Insert

   TRAV13-1 (TRAV13-1 Human)
   

   Expression

   Bacterial

   Mutation

   WT

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTSAR3.1t

   Plasmid

   #210254

   Purpose

   Fluorescent reporter for Type III secretion system activity in Shigella flexneri

   Depositor

   François-Xavier Campbell-Valois

   Article

   Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

   Inserts

   mCerulean
   superfolder GFP
   

   Tags

   ssra

   Expression

   Bacterial

   Promoter

   ipaH7.8p and rpsM

   Available Since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJL1-sfGFP-AQNAT

   Plasmid

   #198211

   Purpose

   pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 30 amino acids containing a non-permissible AQNAT sequon followed by a 6x-His tag

   Depositor

   Michael Jewett

   Article

   Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

   Insert

   sfGFP_AQNAT
   

   Tags

   6x His Tag and AQNAT mutated (non-permissible) gl…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJL1-sfGFP-MOOR

   Plasmid

   #198212

   Purpose

   pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 32 amino acids containing the minimum optimal O-linked recognition site (MOOR) followed by a 6x-His tag

   Depositor

   Michael Jewett

   Article

   Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

   Insert

   sfGFP_MOOR
   

   Tags

   6x His Tag and MOOR glycosylation tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Yellow Cameleon-Nano15/pBig

    Plasmid

    #51962

    Purpose

    Expression of Yellow Cameleon-Nano15, an ultrasensitive Ca2+ indicator with Kd = 15 nM, in Dictyostelium

    Depositor

    Takeharu Nagai

    Article

    Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

    Insert

    Yellow Cameleon-Nano15
    

    Expression

    Bacterial

    Available Since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. XXBLM (XXBLMA-c075)

    Plasmid

    #98223

    Purpose

    Vector for periplasmic expression of Lama antibodies with PelA leader and C-terminal His6 tag

    Depositor

    Nicola Burgess-Brown

    Insert

    XXBLMA (BLM Human)
    

    Tags

    His-6

    Expression

    Bacterial

    Available Since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET15b-Opt13

    Plasmid

    #61698

    Purpose

    Expresses PTDH mutant Opt13

    Depositor

    Huimin Zhao

    Article

    McLachlan et al Biotechnol Bioeng. 2008 Feb 1;99(2):268-

    Insert

    Opt13
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 3, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pLG016 (ApeA)

    Plasmid

    #157894

    Purpose

    Expresses the ApeA defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    ApeA (putative HEPN RNase)
    

    Expression

    Bacterial

    Available Since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. His6-TEV-Venus-pBluescript

    Plasmid

    #166756

    Purpose

    For purification of Venus fluorophore

    Depositor

    David Andrews

    Article

    Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

    Insert

    Venus
    

    Tags

    6x Histidine tag, and TEV protease site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-NLS-M-MLV-RT

    Plasmid

    #181804

    Purpose

    pET28a-NLS-M-MLV RT

    Depositor

    Erik Sontheimer

    Article

    Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

    Insert

    NLS-M-MLV-RT
    

    Expression

    Bacterial

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBL6

    Plasmid

    #50549

    Purpose

    Expresses mCherry under the PLtetO-1 promoter

    Depositor

    Jeffrey Tabor

    Article

    Olson et al Nat Methods. 2014 Mar 9. doi: 10.1038/nm

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSET-GCaMP6f

    Plasmid

    #67564

    Purpose

    bacterial expression of a fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    GCaMP6f
    

    Tags

    HIS-tag

    Expression

    Bacterial

    Available Since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pM9-BetaGal-8C

    Plasmid

    #172489

    Purpose

    Plasmid encoding E. coli BetaGalactosidase with an N-terminal M9 nuclear localization sequence

    Depositor

    Siegfried Musser

    Article

    Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

    Insert

    M9-BetaGal-8C
    

    Tags

    6xHis and M9 nuclear localization sequence (from …

    Expression

    Bacterial

    Mutation

    BetaGal has two cysteine mutations (C77A, C1022S)…

    Promoter

    T7

    Available Since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. KRASB

    Plasmid

    #25153

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    KRASb:M1-K169 (KRAS Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Q61H

    Available Since

    Aug. 6, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

20. pPBO.ACT006

    Plasmid

    #182716

    Purpose

    Constituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region, with eGFP for cloning

    Depositor

    Joseph Schoeniger

    Article

    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

    Insert

    CasRx crRNA cloning backbone
    

    Use

    CRISPR

    Tags

    GFP in cloning site of crRNA for easier screening…

    Expression

    Bacterial

    Mutation

    Catalytically deactivated R295A, H300A, R849A, H8…

    Promoter

    pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)

    Available Since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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