30,296 results

1. His Puc (aa 1-393)

   Plasmid

   #52295

   Purpose

   Bacterial expression of His tagged Drosophila Puc fragment

   Depositor

   Brenda Schulman

   Article

   Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1

   Insert

   Puc (puc Fly)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

2. pBAD-eLACCO2.1

   Plasmid

   #208017

   Purpose

   Biosensor for extracellular L-lactate

   Depositor

   Robert Campbell

   Article

   Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

   Insert

   eLACCO2.1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRMC2-tat:msfGFP

   Plasmid

   #194915

   Purpose

   Tetracycline inducible expression of msfGFP fused to Tat signal peptide in Staphylococci

   Depositor

   Rikke Meyer

   Article

   Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.

   Insert

   Shine Dalgarno sequence followed by Tat signal peptide fused to monomeric superfolder GFP
   

   Use

   Tetracycline-inducible expression vector for stap…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28_Caspase-4_K356D

   Plasmid

   #214312

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   CASP4 (CASP4 Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   K356D

   Promoter

   T7

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Affibody HER2:243.BirA*-pET301/NT-DEST

   Plasmid

   #174692

   Purpose

   Plasmid encoding for the bacterial expression of a bispecific coding for the anti-HER2 affibody ZHER2:342 fused to the mutated form of BirA enzyme, BirA*

   Depositor

   Jean Gariepy

   Article

   Alwash et al ACS Pharmacol Transl Sci. 2022 Feb 1;5(2

   Insert

   Anti-HER2 affibody ZHER2:342 fused to mutated form of BirA, BirA*
   

   Use

   Tags

   HIS tag

   Expression

   Bacterial

   Mutation

   R118G mutation in BirA

   Promoter

   T7 promoter

   Available since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSt-KO

   Plasmid

   #44566

   Purpose

   for generation of knockout of mycobacterial genes

   Depositor

   Vinay Nandicoori

   Article

   Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

   Type

   Empty backbone

   Use

   E.coli-mycobacteria shuttle vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Human RNF144B (27-236)

   Plasmid

   #110753

   Purpose

   Bacterial Expression of RNF144B RBR domain

   Depositor

   David Komander

   Article

   Michel et al Mol Cell. 2017 Oct 5;68(1):233-246.e5. d

   Insert

   RNF144B (RNF144B Human)
   

   Use

   Tags

   N-His6-GST-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTrc99S-ssDsbA-MBP-4xDQNAT

   Plasmid

   #128398

   Purpose

   Periplasmic expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites in E. coli

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Insert

   Maltose binding protein
   

   Use

   Tags

   4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

   Expression

   Bacterial

   Mutation

   Y342H- please see depositor comments

   Promoter

   trc

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAN-PTet-dCas9

   Plasmid

   #62244

   Purpose

   controls the expression of S. pyogenes dCas9 from a Tc‐inducible PTet promoter.

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   catalytically inactive, D10A, H840A

   Promoter

   Ptet

   Available since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 6xHis-MBP-huBoCas12a

    Plasmid

    #174673

    Purpose

    Expresses 6xHis-MBP tagged BoCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huBoCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. SpyCatcher002-oHex

    Plasmid

    #124670

    Purpose

    Expresses SpyCatcher002-oHex for hexamerization of SpyTag-proteins via hexameric coiled-coil

    Depositor

    Mark Howarth

    Article

    Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

    Insert

    SpyCatcher002-oHex
    

    Use

    Tags

    C-tag, His6, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTYB3-PUM1-HD

    Plasmid

    #17543

    Depositor

    Traci Hall

    Article

    Wang et al Mol Cell. 2001 Apr . 7(4):855-65.

    Insert

    human PUM1 Pumilio homology domain (PUM1 Human)
    

    Use

    Tags

    intein

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3.1MycHis/HIP1

    Plasmid

    #29590

    Depositor

    Theodora Ross

    Article

    Rao et al J Clin Invest. 2002 Aug . 110(3):351-60.

    Insert

    Huntingtin-interacting protein 1 (HIP1 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSG28-J23151-AraC

    Plasmid

    #102453

    Purpose

    Expresses AraC protein in E. coli. The expression is controlled by a constitutive promoter.

    Depositor

    Richard Murray

    Article

    Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

    Insert

    AraC (araC )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23151

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pmSod1-WT-N’His

    Plasmid

    #117701

    Purpose

    Expression of WT mouse SOD1 with N- terminal His tag

    Depositor

    Elizabeth Fisher

    Insert

    Sod1 (Sod1 Mouse)
    

    Use

    Tags

    His tag with Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET-DUET-1-mRhubarb713-HO

    Plasmid

    #141199

    Purpose

    Expresses mRhubarb713 fluorescent protein and heme oxygenase

    Depositor

    Elad Firnberg

    Article

    Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

    Inserts

    mRhubarb713
    heme oxygenase
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    R134H, W309R, Q310A compared to iRFP

    Promoter

    T7

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSMT-MjtRNA-GFP(47TAG)

    Plasmid

    #193251

    Purpose

    Expresses the M. jannaschii tRNATyr (MjtRNA) and GFP(47TAG) (aka GFP151TAG)

    Depositor

    Peter Schultz, Jessica Seeliger

    Article

    Touchette et al ACS Infect Dis. 2017 May 12;3(5):336-348

    Inserts

    GFP(47TAG)
    MjtRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon for Tyr47 replaced with amber stop codon TA…

    Promoter

    hsp60 and metU

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNIC28-Bsa4_ZV-NS5-RdRp

    Plasmid

    #179847

    Purpose

    The RdRp domain (with a 6-histidine tag) from Zika virus nonstructural protein 5 (French Polynesia strain, GenBank accession number ARU07075.1) encompassing residues 270 to 89

    Depositor

    Julien Lescar

    Article

    Gharbi-Ayachi et al J Virol. 2020 Oct 14;94(21). pii: JVI.00

    Insert

    Zika Virus Non-structural protein 5 RdRp domain
    

    Use

    Tags

    6-histine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a-mTagBFP2-TEV-Strep-His

    Plasmid

    #210824

    Purpose

    Bacterial overexpression vector for C-terminally Strep and His tagged mTagBFP2 fluorescent protein

    Depositor

    Luke Chao

    Article

    Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

    Insert

    mTagBFP2
    

    Use

    Tags

    TwinStrep, 10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p4027 GST-ubiquitin

    Plasmid

    #10861

    Depositor

    Peter Howley

    Article

    Scheffner et al Cell. 1993 Nov 5. 75(3):495-505.

    Insert

    ubiquitin (UBI4 Budding Yeast)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only