33,123 results
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Plasmid#73194PurposeTemplate plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (mqsR) under the control of rhamnose induceable promoter (PrhaB) for negative selection.DepositorAvailable SinceJune 30, 2016AvailabilityAcademic Institutions and Nonprofits only
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PGEX 4T-1-PPARgamma2
Plasmid#78773PurposeTo purify GST-tagged recombinant proteinDepositorInsertPPAR_2
TagsGSTExpressionBacterialPromotertacAvailable SinceJune 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHlyIIR-H1
Plasmid#74681PurposeHlyIIR-H1 gateDepositorInsertPTac-RBSH1-HlyIIR-PHlyIIR-YFP
ExpressionBacterialAvailable SinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVEX-CC1
Plasmid#74170Purposeprotein expression of chicken DCTN1 p150Glued AA 217-548 (CC1) in bacteriaDepositorAvailable SinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAmtR-A1
Plasmid#74676PurposeAmtR-A1 gateDepositorInsertPTac-RBSA1-AmtR-PAmtR-YFP
ExpressionBacterialAvailable SinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAK1
Plasmid#73244PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertAAK1 (AAK1 Human)
TagsTEV-His6-FlagExpressionBacterialMutationpfam00069:Pkinase 47-307PromoterT7Available SinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAPK3
Plasmid#73235PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMAPK3 (MAPK3 Human)
TagsHis-6-TEVExpressionBacterialMutationpfam00069:Pkinase 42-330PromoterT7Available SinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAL-pgi_NT
Plasmid#74067PurposepAL374 plasmid carrying the pgi (NT) sgRNA, targeting the non-template strand of pgi, SpecRDepositorInsertsgRNA pgi (NT)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterptacAvailable SinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAL-pyk_NT
Plasmid#74072PurposepAL374 plasmid carrying the pyk (NT) sgRNA targeting the non-template strand of pyk, SpecRDepositorInsertsgRNA pyk (NT)
UseCRISPRExpressionBacterialPromoterptacAvailable SinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-human MIZ1 wild type
Plasmid#74161Purposebacterial expression of MIZ1DepositorAvailable SinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKS011
Plasmid#65464PurposepSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) MBP (Maltose Binding Protein), Ntag, and mCherryDepositorInsertMBP-Ntag-mCherry
TagsFLAG and MBPExpressionBacterialPromoterpl-TetOAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK407
Plasmid#72421PurposeProduces Acetobacter aceti 1023 Est06, C-terminal His6 tagDepositorInsertlipase
TagsHis6ExpressionBacterialMutationlacks Met1 and Thr2 in annotated (v1) sequencePromoterT7Available SinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
TOPO GFP
Plasmid#68488PurposeTOPO construct expressing GFP for generation of GMAP-compatible geneDepositorInsertGreen Fluorescent Protein
UseGmapExpressionBacterialPromoterPlacAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
TOPO mCherry
Plasmid#68490PurposeTOPO construct expressing mCherry for generation of GMAP-compatible geneDepositorInsertmCherry
UseGmapExpressionBacterialPromoterPlacAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK645
Plasmid#71700PurposeProduces E. coli thioredoxin (EcTrxA)DepositorInsertthioredoxin
ExpressionBacterialPromoterT7Available SinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
Aurein1.2_(pos36GFP)-Cys
Plasmid#71757PurposeAurein1.2_+36GFP-Cys expression in E.ColiDepositorInsertAurein1.2_(pos36_GFP)-Cys
Tags6XHisExpressionBacterialAvailable SinceJan. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HsSENP7
Plasmid#71470PurposeEncodes human full length SENP7. Suitable for in vitro transcription/translation (T7).DepositorInsertSUMO1/sentrin specific peptidase 7 (SENP7 Human)
TagsHexahistidineExpressionBacterialMutationGene optimised for E. coli expression (Genscript)PromoterT7Available SinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTKDP-hph
Plasmid#71322PurposeDonor plasmid - hygromycin resistant donor cassetteDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET23a-HsUBB
Plasmid#69562PurposeBacterial expression; in vitro transcription/translation (T7)DepositorAvailable SinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc3-HISNDHFR
Plasmid#63976PurposePosition 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneTags6xHis; TEV cleavableExpressionBacterialPromoterT7Available SinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only