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Plasmid#211789PurposeExpression plasmid in E. coli BL21(DE3) , which can be used for expression and purification to get protein LolT_C41SDepositorInsertlolt mutant
UseTagsHis tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUltra-FPheKRS
Plasmid#136631PurposeGenetic Incorporation 4-fluorophenyl carbamate lysine (FPheK) into proteinsDepositorInsertFPheKRS
UseTagsExpressionBacterialMutationPromoterTac1 promoterAvailable sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-APEX2
Plasmid#109420PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to ascorbate peroxidase 2. VHH-APEX2 also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, APEX2, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, and T7ExpressionBacterialMutationPromoterT7Available sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pST39-Ubc13-MMS2
Plasmid#184807PurposeBacterial expression of yeast UEV-E2 complexDepositorInsertUbc13, MMS2
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
ELF3_pet28a
Plasmid#131658PurposeBacterial expression of ETS gene ELF3DepositorInsertELF3 (ELF3 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDB180
Plasmid#80677Purposefor secretion of N-terminal OsmY, deca-histidine, SUMO tagged proteinDepositorTypeEmpty backboneUseTagsOsmY-10His-Smt3ExpressionBacterialMutationPromoterT7Available sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
PXWABS62(E11-TEV)
Plasmid#123157PurposeFor TEV-based leakiness regulation. pSB4A3 carrying ParsR-ABS62-32ECF11-t-Pecf11-32malE-te-TEV-tDepositorInsertParsR-ABS62-32ECF11-t-Pecf11-32malE-te-TEV-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterParsR-ABS62, Pecf11Available sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGLO-Pink-SHE
Plasmid#220781PurposeExpresses a raspberry chromoprotein (visible light and fluorescence) with a his-tag and an inducible GFP (araBAD-promoter)DepositorInsertraspberry chromoprotein (mRFP1)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJBL2807
Plasmid#71203PurposeAD1 STAR Antisense Expression PlasmidDepositorInsertAD1 Antisense
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterBBa_J23119Available sinceJan. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJBL2801
Plasmid#71207PurposeAD1 Sense Expression PlasmidDepositorInsertAD1 Sense
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterBBa_J23119Available sinceJan. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCas-dCas12m
Plasmid#192275PurposeEncodes MmdCas12m (D485A) under a constitutive promoterDepositorInsertMmdCas12m
UseCRISPRTagsExpressionBacterialMutationD485APromoterPJ23108Available sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSA2
Plasmid#52262PurposeBacterial expression of human 6His-tagged SUMO2 and SUMO-E1DepositorUseTags6His-tagExpressionBacterialMutationC-terminal gly-glyPromoterT7 and rbsAvailable sinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX-GST-AGO1
Plasmid#24316DepositorInserthuman AGO1 (AGO1 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceApril 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
NFAT5 luciferase
Plasmid#14110DepositorInsertNM_001113178.2 (NFAT5 Human)
UseLuciferaseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET24c(+)-mtEFTu
Plasmid#30535DepositorInsertMature bovine mitochondrial elongation factor Tu
UseTagsHisExpressionBacterialMutationMature form amino acids 44-452. Mito import seque…PromoterAvailable sinceOct. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSAN-QUE37C
Plasmid#129312PurposeATP measurementDepositorInsertQUEEN-37C
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH474_pTech_Para-supP
Plasmid#163718PurposeArabinose-inducible expression of supPDepositorInsertsupP
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1_GST-PLproCS-MBP
Plasmid#169195PurposeTo express a PLpro substrate (GST-PLproCS-MBP) in E. coliDepositorInsertGST-PLproCS-MBP
UseTagsGST-MBP fusion (PLpro-cleavable) and His6 (intern…ExpressionBacterialMutationPromotertac promoterAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pD434-SR mouse Alpha-synuclein
Plasmid#89073Purposebacterial expression of mouse Alpha-synucleinDepositorInsertAlpha-synuclein (Snca Mouse)
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 17, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a(+)::NL3F10H
Plasmid#141290PurposeNLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)DepositorInsertNLUC3F10H
UseLuciferaseTagsFlag (x3) and His (x10)ExpressionBacterialMutationEngineered for high stability (t1/2 = 11.5 days a…Promoterpromoter for bacteriophage T7 RNA polymeraseAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only