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31,059 results

Showing: 5861 - 5880 of 31059 results

  3. pPGC-C

    Plasmid
    #174580
    Purpose
    pOPIN-GG expression vector compatible with C-terminal tagging, carbenicillin resistant
    Depositor
    Mark Banfield
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    Sept. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    174580-plasmid-map-sequence-id-342965

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  4. pYFP

    Plasmid
    #175592
    Purpose
    Tet inducible, KAN Resistant, mVenus gene on a low copy backbone. Used as a negative control to the pRelA_YFP plasmid.
    Depositor
    Greg Bokinsky
    Article
    Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.
    Insert
    mVenus
    Expression
    Bacterial
    Mutation
    An improved monomeric YFP. See doi: 10.1021/bi051…
    Available Since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    175592-plasmid-map-sequence-id-348320

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  5. KRASB

    Plasmid
    #25153
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    KRASb:M1-K169 (KRAS Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Q61H
    Available Since
    Aug. 6, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  6. pcDNA.3.1.ADRB2-NP

    Plasmid
    #134376
    Purpose
    Nanoluc complementation assay. Expression of adrenoceptor beta 2 fused at C termimus with Natural peptide (NP) of NanoLuc. Addition of signal sequence and Flag epitope at N terminus of ADRB2.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    ADRB2-NP (ADRB2 Human)
    Tags
    Flag, natural peptide of nanoluciferase, and sign…
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available Since
    Aug. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134376-plasmid-map-sequence-id-306396

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  17. MSP712

    Plasmid
    #65768
    Purpose
    Bacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Mutation
    D10A and H840A mutations in Cas9
    Promoter
    T7 (x2)
    Available Since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65768-plasmid-map-sequence-id-112655

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Showing: 5861 - 5880 of 31059 results