32,069 results
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Plasmid#98917PurposeConstitutively expresses the CaPR construct, the voltage indicator PROPS tethered to the calcium sensor GCaMP6f, under the Sigma 70 promoter in E. coli.DepositorInsertCaPR
ExpressionBacterialPromoterBBa_J23118 - Strong ConstitutiveAvailable SinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
Spacer Switch Plasmid
Plasmid#215346PurposeFacilitates the change in spacer sequence to Neisseria gonorrhoeae encoding the Nla CRISPRi system.DepositorInsertOpaD spacer; Chloramphenicol Resistance Cassette
UseCRISPRExpressionBacterialAvailable SinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pL-RPB1
Plasmid#10981DepositorAvailable SinceDec. 16, 2005AvailabilityAcademic Institutions and Nonprofits only -
HHM-iATPSnFR1.1
Plasmid#102547Purposeexpresses ATP sensor in bacteriaDepositorInsertiATPSnFR1.1
ExpressionBacterialAvailable SinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEvoltac-EcW-TGA-h14
Plasmid#218764Purposeencodes EcWRS-h14 and EcW-tRNA-TGADepositorInsertEcWRS-h14
ExpressionBacterialMutationS8A, V144G, V146CPromotertacIAvailable SinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrA
Plasmid#216229PurposeS. aureus USA300 GyrA Codon-optimized for E. coliDepositorInsertDNA gyrase
Tags6xHisExpressionBacterialPromoterT7Available SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBbS8c-ddcpf1-Δ
Plasmid#153039PurposeFor CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array lacking a gene-specific spacer.DepositorInsertAscpf1
ExpressionBacterialMutationE993AAvailable SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
spCas9-NLS-MAV
Plasmid#124212PurposeSpCas9-NLS 17 amino acid linker to monoavidin (SpCas9-NLS-MAV)DepositorInsertcas9
UseCRISPRTagsHis6, MBP, TEV Cleavage site, and two copies of S…ExpressionBacterialPromoterT7Available SinceJune 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pASTA3
Plasmid#24657DepositorInserttdTomato
ExpressionBacterialAvailable SinceJune 9, 2010AvailabilityAcademic Institutions and Nonprofits only -
pNHEJ-Cas12a
Plasmid#158713PurposeCRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis, expresses FnCpf1, NHEJ machinery of M. marinumDepositorInsertthe MmNHEJ machinery (MMAR_4573, MMAR_4574, and MMAR_4575)
UseCRISPRExpressionBacterialAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBK-EcTrpRS(H14)-EcTyrRS(VSMA,D165G)
Plasmid#231130PurposeExpresses mutant E. coli TrpRSTyrRS from a glnS promoters for proteome-wide incorporation of tryptophan/tyrosine analogues.DepositorInsertE. coli tyrosyl tRNA synthetase
ExpressionBacterialMutationEcTrpRS: S8A, V144G, V146C; EcTyrRS: Y37V, D165G,…Available SinceOct. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_Uricase
Plasmid#182400PurposeBacterial expression of uricaseDepositorInserturicase
ExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSPORT1[attB/Ins1/NbT promoter/glGFP/bglobin 3'-UTR/Ins2]
Plasmid#74102Purposeplasmid driven under neuronal beta-tubulin promoter, bearing attB and two insulator sequences (opposite directions pointing inward towards the reporter gene)DepositorInsertsXenopus laevis beta-tubulin gene promoter region and 5'UTR
Green Lantern Green Fluorescent Protein
Rabbit beta 1-globin gene 3'UTR
ExpressionBacterialAvailable SinceMay 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET50_MDA5∆2CARD
Plasmid#216804PurposeExpresses melanoma differentiation-associated protein 5 lacking its 2 CARD domainsDepositorInsertmelanoma differentiation-associated protein 5 delta 2 CARD domains (IFIH1 Human)
ExpressionBacterialMutation∆1-287Available SinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pALACE
Plasmid#31853DepositorTypeEmpty backboneUseEscherichia coli-mycobacterium shuttle plasmidTags6xHISExpressionBacterialPromoterAcetamidase operoneAvailable SinceJuly 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTYB2-eIF1A
Plasmid#37218DepositorInserteIF1A
ExpressionBacterialPromoterT7Available SinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDule- Mj Acd A9
Plasmid#197651PurposeE. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pairDepositorInsertsMj Acd A9 synthetase
Methanocaldococcus jannaschii tRNA
TagsNoneExpressionBacterialMutationY32A, L65D, F108L, Q109E, D158S, L162TPromoterGlnS and lppAvailable SinceApril 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
EcAvs4
Plasmid#188794PurposeAvs4 from Escherichia coli NCTC11132DepositorInsertEcAvs4
ExpressionBacterialAvailable SinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEB1-moxGFP
Plasmid#103984PurposePlasmid encoding moxGFPDepositorInsertmoxGFP
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (S30R, Y39N, …PromoterproCAvailable SinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLG021 (AVAST type 5)
Plasmid#157899PurposeExpresses the AVAST type 5 defense systemDepositorInsertAVAST type 5 (STAND ATPase with N-terminal SIR2)
ExpressionBacterialAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only