30,296 results

1. pCK01_PalkS_alkS

   Plasmid

   #166502

   Purpose

   Expression of AlkS transcriptional regulator with L401G mutation. Sensor input plasmid for detection of n-butanol and branched-chain higher alcohols.

   Depositor

   Sven Panke

   Article

   Bahls et al Metab Eng. 2021 Nov 9. pii: S1096-7176(2

   Insert

   alkS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   L401G

   Promoter

   PalkS

   Available since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAB_PalkB_sfgfp_KanR

   Plasmid

   #166503

   Purpose

   Expression of sfGFP under control of PalkB promoter. Sensor output plasmid for detection of n-butanol and branched-chain higher alcohols.

   Depositor

   Sven Panke

   Article

   Bahls et al Metab Eng. 2021 Nov 9. pii: S1096-7176(2

   Insert

   sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PalkB

   Available since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTcI-Wasabi

   Plasmid

   #86101

   Purpose

   mWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI

   Depositor

   Mikhail Shapiro

   Article

   Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

   Inserts

   TcI
   mWasabi
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A67T

   Promoter

   pR/pL and pR/pL, LacI

   Available since

   Feb. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21-pelB-LaM-3

   Plasmid

   #172769

   Purpose

   Bacterial expression of anti-mCherry nanobody LaM-3, with pelB leader and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaM-3
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMV361_MjTyrRS

   Plasmid

   #193250

   Purpose

   Expresses the M. jannaschii tyrosyl-tRNA synthetase (MjTyrRS)

   Depositor

   Peter Schultz, Jessica Seeliger

   Article

   Touchette et al ACS Infect Dis. 2017 May 12;3(5):336-348

   Insert

   MjTyrRS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   hsp60

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTK204

   Plasmid

   #13534

   Depositor

   Wolf Frommer

   Article

   Kaper et al PLoS Biol. 2007 Oct;5(10):e257.

   Insert

   FLIPW-CTYT
   

   Use

   Tags

   See map

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 27, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. KPC2-pNic-Bsa4

   Plasmid

   #231082

   Purpose

   expresses Klebsiella pneumoniae carbapenemase-2 (KPC-2) under T7 with His tag

   Depositor

   Josh Beckham

   Insert

   carbapenemase-2 (kpc2 )
   

   Use

   Tags

   6x His tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBP-P_tetR-PTet*

   Plasmid

   #204077

   Purpose

   Type 0 promoter part

   Depositor

   Mikhail Shapiro

   Insert

   tetR repressor and PTet* tetracycline-inducible promoter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TTR C10S S46C

   Plasmid

   #190008

   Purpose

   Expression of human TTR in E coli

   Depositor

   Peter Wright

   Article

   Sun et al J Biol Chem. 2022 Jun 18;298(8):102162.

   Insert

   TTR (TTR Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C10S, S46C

   Promoter

   T7

   Available since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBEL1994

    Plasmid

    #139898

    Purpose

    Expression plasmid for LetB PLL2 deletion (without TM region)

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetB(43-877; delta229-239, D228G, L240G)-6xHis

    Promoter

    Available since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Pf-ClpP

    Plasmid

    #25591

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    Pf-ClpP
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 25, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pMBP-HIS2-Vps20FL

    Plasmid

    #21490

    Depositor

    James Hurley

    Article

    Wollert et al Nature. 2009 Mar 12. 458(7235):172-7.

    Insert

    VPS20 (VPS20 Budding Yeast)
    

    Use

    Tags

    6x His and MBP

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    Sept. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pC0075 TtCsm6 His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115270

    Purpose

    Expresses TtCsm6 from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    TtCsm6
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKFSS1_2

    Plasmid

    #118227

    Purpose

    Empty E. coli - B. burgdorferi shuttle vector; streptomycin resistant

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEX-KG-Itch/2793

    Plasmid

    #11434

    Depositor

    Allan Weissman

    Article

    Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

    Insert

    Itch (Itch Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    n/a

    Promoter

    Available since

    April 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. Gfp-zf-vasa

    Plasmid

    #167323

    Purpose

    Plasmid for synthesis of GFP chimeric RNA that contained vasa-3'UTRs of zebrafish by in vitro transcription.

    Depositor

    Goro Yoshizaki

    Article

    Yoshizaki et al Biol Reprod. 2005 Jul;73(1):88-93. doi:

    Insert

    zf vasa 3'UTRs (ddx4 Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBAD/HisD-PATagRFP

    Plasmid

    #31945

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al J Am Chem Soc. 2010 May 12;132(18):6481-

    Insert

    PATagRFP
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    R69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…

    Promoter

    araB

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. His-SUMO-hDDI2-D252N_pET11a

    Plasmid

    #169122

    Purpose

    expression of protein

    Depositor

    Barbara Dirac-Svejstrup

    Article

    Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

    Insert

    hDDI (DDI2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D252N

    Promoter

    Available since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAJM.683

    Plasmid

    #108531

    Purpose

    BetIAM + PBetI-YFP Reporter. Cho sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    BetIAM + PBetI-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    BetI: S115I

    Promoter

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. RHOAA-c033

    Plasmid

    #205116

    Purpose

    RHOA protein expression

    Depositor

    Paul Brennan

    Insert

    RHOA (RHOA Human)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge