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nuc-mCherry-pTRIP

Plasmid

#163520

Purpose

Used to construct lentivirus to express nucleus-targeted mCherry in mammalian cells

Depositor

Thomas Weber

Insert

nucleus targeted mCherry

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCDFDuet-1 MKK4(EE)-MKK7a1(EE)

Plasmid

#47580

Purpose

Bacterial expression plasmid for co-expression of constitutively active MKK4 and MKK7a1

Depositor

Kevin Janes

Article

Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

Inserts

MKK4-EE (MAP2K4 Human)
MKK7a1-EE (Map2k7 Mouse)

Expression

Bacterial

Mutation

Lacks the first 36 residues of the RefSeq protein…

Available Since

Sept. 19, 2013

Availability

Academic Institutions and Nonprofits only
pBAD-D2

Plasmid

#37470

Depositor

Amy Palmer, Roger Tsien

Article

Palmer et al Chem Biol. 2006 May;13(5):521-30.

Insert

D2 second generation cameleon (calcium sensor)

Expression

Bacterial

Promoter

Ara

Available Since

Oct. 11, 2012

Availability

Academic Institutions and Nonprofits only
pBS mPrx1 (CT#640)

Plasmid

#13961

Depositor

Cliff Tabin

Article

Logan et al Genesis. 2002 Jun . 33(2):77-80.

Insert

Prx1 promoter (Prrx1 Mouse)

Expression

Bacterial

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pET21b-Caspase-3(V266E)

Plasmid

#90089

Purpose

Expresses caspase 3 V266E mutant in bacterial cells

Depositor

Clay Clark

Article

Pop et al Biochemistry. 2003 Oct 28;42(42):12311-2

Insert

Caspase 3 (CASP3 Human)

Tags

His tag

Expression

Bacterial

Mutation

V266E

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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TJP1_PDZ_2

Plasmid

#103910

Purpose

Protein expression and purification of ZO1 PDZ2 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

TJP1 (TJP1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX Emt-SH2

Plasmid

#46424

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Emt (ITK Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 223-346

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX-PPP1R12A wt

Plasmid

#31669

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

Protein Phosphatase 1 Regulatory Subunit 12A (PPP1R12A Human)

Tags

GST

Expression

Bacterial

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pET-Duet-1-NONO(53-312)

Plasmid

#135442

Purpose

Expresses NONO 53-312 in the second site; for co-expression with His-tagged protein

Depositor

Charles Bond

Article

Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

Insert

Non-POU domain-containing octamer-binding protein (NONO Human)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pBI121::smEclipGFP:AQ

Plasmid

#240454

Purpose

Expression of indicator protein fusion (soluble modified ecliptic pHluorin & Aequorin) for monitoring calcium concentrations and pH in the cytoplasm of higher plants. See Resource Information.

Depositor

Christoph Plieth

Article

Gao et al Plant Physiol. 2004 Mar;134(3):898-908.

Insert

Fusion of soluble modified ecliptic pHluorin and Aequorin

Expression

Bacterial and Plant

Mutation

Ecliptic GFP (ecliptic pHluorin) (PMID 9671304); …

Promoter

Cauliflower mosaic virus 35S promoter (GenBank AJ…

Available Since

Sept. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pHH0103_SFN_14-3-3

Plasmid

#109937

Purpose

Protein expression and purification of SFN_14-3-3

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

SFN_14-3-3

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-eGFP-MinD

Plasmid

#133622

Purpose

Expression vector for the Escherichia coli MinD with an N-terminal fusion of a His-tag for purification and an EGFP for visualization.

Depositor

Petra Schwille

Article

Zieske et al FEBS Lett. 2014 Aug 1;588(15):2545-9. do

Insert

EGFP-MinD (minD E. coli)

Tags

His

Expression

Bacterial

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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CP2-TCC

Plasmid

#179679

Purpose

Complementary plasmid

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

[sd2] T7 RNAP-TCC linker-degron

Expression

Bacterial

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pTlpA_mCherry_YafQ/DinJ

Plasmid

#229148

Purpose

Toxin-Antitoxin (Dinj/YafQ)

Depositor

Shrikrishnan Sankaran

Article

Dey et al Microb Biotechnol. 2023 Jun;16(6):1264-1

Insert

mCherry, DinJ/YafQ

Expression

Bacterial

Promoter

PtlpA (mCherry), DinJ/YafQ (Native)

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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human p53-(1-320)

Plasmid

#24864

Depositor

Cheryl Arrowsmith

Article

Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Contains aa 1-320

Available Since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only
6xHis-MBP-TEV-mDvl2PDZ(264-353)_pCDFduet

Plasmid

#216386

Purpose

Bacterial expression vector for MBP-tagged mouse Dvl2 PDZ domain (residues 264-353)

Depositor

William Weis

Article

Mahoney et al Sci Signal. 2022 Aug 23;15(748):eabo2820

Insert

Dishevelled-2 (Dvl2 Mouse)

Tags

6xHis-MBP-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET28C-SARS-CoV-2-Nucleocapsid

Plasmid

#204402

Purpose

E. coli expression of full-length SARS-CoV-2 nucleocapsid

Depositor

Samie Jaffrey

Article

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

SARS-CoV-2 nucleocapsid (N SARS-CoV-2)

Tags

6x-His

Expression

Bacterial

Promoter

T7

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pET_MBP-RGG-GFP-RGG

Plasmid

#124949

Purpose

Expression of MBP-RGG-GFP-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

MBP-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

EGFP, His, and MBP

Expression

Bacterial

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pAIP4_3KB5

Plasmid

#234225

Purpose

Heterologous protein expression of tripartite motif-containing protein 72 (Mg53) in Escherichia coli

Depositor

Christopher Bahl

Insert

3KB5 (TRIM72 Human)

Tags

10x HisTag-Smt3

Expression

Bacterial

Promoter

T7

Available Since

March 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pCM271rfp

Plasmid

#59774

Purpose

pCMrfp with TrfA mutation (271R to C)

Depositor

Nathan Hillson

Article

Bi et al Microb Cell Fact. 2013 Nov 13;12:107. do

Insert

RFP

Expression

Bacterial

Promoter

pBAD

Available Since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

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