30,306 results

1. pETDuet-LahT150

   Plasmid

   #166785

   Purpose

   Cleavage of double glycine motif in peptides to remove the leader sequence

   Depositor

   Wilfred van der Donk

   Article

   Bobeica et al Elife. 2019 Jan 14;8. pii: 42305. doi: 1

   Insert

   LahT150
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHH0103_ESRRA_Hormone-recep

   Plasmid

   #109908

   Purpose

   Protein expression and purification of ESRRA_Hormone-recep

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   ESRRA_Hormone-recep (ESRRA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pETSG

   Plasmid

   #159418

   Purpose

   Expression of proteins with a C-terminal thermostable GFP (TGP) tag in Escherichia coli

   Depositor

   Dianfan Li

   Article

   Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

   Insert

   thermostable GFP (TGP)
   

   Use

   Tags

   polyhistidine (poly-His)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Kindlin

   Plasmid

   #186135

   Purpose

   For expression of recombinant kindlin in bacteria

   Depositor

   Michael Rosen

   Article

   Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

   Insert

   FERMT2 (FERMT2 Human)
   

   Use

   Tags

   His6-sumo

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. BMP2K

   Plasmid

   #73251

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   BMP2KA (BMP2K Human)
   

   Use

   Tags

   His6-Zb-TEV

   Expression

   Bacterial

   Mutation

   pfam00069:Pkinase 52-311

   Promoter

   T7

   Available since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pEB2-DsRed-Express2

   Plasmid

   #103999

   Purpose

   Plasmid encoding DsRed-Express2

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   DsRed-Express2
   

   Use

   Low copy

   Tags

   Expression

   Bacterial

   Mutation

   Mutations relative to DsRed (R2D, S4T, K5E, E10P,…

   Promoter

   proC

   Available since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJJGL002

   Plasmid

   #180606

   Purpose

   Plasmid expressing E. coli codon optimized His-MBP-TEV-PlmCasX

   Depositor

   Jennifer Doudna

   Article

   Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

   Insert

   PlmCasX
   

   Use

   CRISPR

   Tags

   10xHis and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pVRa42_4454

   Plasmid

   #49703

   Depositor

   Christopher Voigt

   Article

   Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

   Insert

   ECF42_4454
   

   Use

   Synthetic Biology

   Tags

   His6-PreScission

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pT7-lacO

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFE-Selo-FI-prk

   Plasmid

   #162698

   Purpose

   Expresses S. elongatus Form IB rubisco and S. elongatus Prk

   Depositor

   David Savage

   Article

   Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

   Insert

   S. elongatus Form IB rubisco and S. elongatus Prk
   

   Use

   Tags

   N terminal 6x His tag on PRK

   Expression

   Bacterial

   Mutation

   Promoter

   PLtet0-1 promoter

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGST-PPARgamma

    Plasmid

    #16549

    Depositor

    Bert Vogelstein

    Article

    He et al Cell. 1999 Oct 29. 99(3):335-45.

    Insert

    PPAR gamma (PPARG Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    N-terminal DNA-binding domain: aa 1-248

    Promoter

    Available since

    March 24, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGHUC w-ERBIN (2-hybrid prey plasmid)

    Plasmid

    #128163

    Purpose

    UV5 promoter drives expression of omega-erbin fusion. Pair with pB1H2 UV5 Zif268-cons as a positive control (turns on HIS3/GFP).

    Depositor

    Marcus Noyes

    Article

    Ichikawa et al ACS Synth Biol. 2019 May 17;8(5):918-928

    Insert

    ERBIN PDZ domain (ERBIN Synthetic)
    

    Use

    Synthetic Biology

    Tags

    FLAG and Omega subunit of RNA polymerase

    Expression

    Bacterial

    Mutation

    Promoter

    UV5

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pROP-IP-FBFP

    Plasmid

    #122138

    Purpose

    FMN-based fluorescent protein Reporter plasmid for B-Rex contain imperfect operator sequence (anaerobic purpose)

    Depositor

    Srivatsan Raman

    Article

    Liu et al ACS Synth Biol. 2019 Feb 15;8(2):264-273

    Insert

    FBFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101

    Available since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GFP-X0

    Plasmid

    #115565

    Purpose

    Control vector expressing GFP alone without any consensus repeats

    Depositor

    Michael Downey

    Article

    Rossl et al J Biol Chem. 2019 Apr 19;294(16):6227-62

    Insert

    Green Fluorescent Protein
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. SOCS2A-c016

    Plasmid

    #53689

    Purpose

    bacterial expression plasmid

    Depositor

    Nicola Burgess-Brown

    Insert

    SOCS2A
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Bacterial expression for structure determination;…

    Promoter

    Available since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pDNA2.0 6H-TEV-KRAS G13D

    Plasmid

    #111851

    Purpose

    Expresses activated human KRAS G13D (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.

    Depositor

    Kenneth Westover

    Article

    Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.

    Insert

    KRAS G13D (KRAS Human)
    

    Use

    Tags

    6xHis-tag and TEV protease cleavage sequence

    Expression

    Bacterial

    Mutation

    Changed Glycine 13 to Aspartic Acid

    Promoter

    T5

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. Nano-lantern/pRSETB

    Plasmid

    #51969

    Purpose

    bacterial expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    S257G in Rluc8

    Promoter

    Available since

    March 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Pet28a-His-tag-BS2

    Plasmid

    #220347

    Purpose

    E. coli expression vector to purify BS2

    Depositor

    Bryan Dickinson

    Article

    Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

    Insert

    BS2
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pMBEC8

    Plasmid

    #187599

    Purpose

    Base editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Apramycin resistance

    Depositor

    Pablo Ivan Nikel

    Article

    Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:

    Insert

    nCas9-BEC-UGI; cas6f, gfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSBY1_FnCpf1cg

    Plasmid

    #104622

    Purpose

    CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn CPf1

    Depositor

    Sheng Yang

    Article

    Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

    Insert

    FnCpf1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    codon-optimized C.glutamicum

    Promoter

    hsp60

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTrcECT

    Plasmid

    #75477

    Purpose

    Inducible expression of ectABC in E. coli

    Depositor

    Xixian Xie

    Article

    Ning et al Metab Eng. 2016 Mar 9;36:10-18. doi: 10.

    Insert

    ectABC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    May 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge