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VV020: WT Arch-3 - eGFP in pET28b

Plasmid

#58488

Purpose

Expresses WT Archaerhodopsin-3 fused to eGFP (with 6x C-terminal His tag) in E. coli

Depositor

Adam Cohen

Article

Maclaurin et al Proc Natl Acad Sci U S A. 2013 Apr 9;110

Insert

Archaerhodopsin-3

Use

Tags

eGFP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pEB2-2ndVal mCherry2-L

Plasmid

#104027

Purpose

Plasmid encoding 2ndVal mCherry2-L

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal mCherry2-L

Use

Low copy

Tags

Expression

Bacterial

Mutation

mCherry2-L with valine inserted in 2nd position

Promoter

proC

Available since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pNatA (pACYCduet-naa10-naa15)

Plasmid

#72928

Purpose

Allows expression of a modified version of the fission yeast NatA complex - chloramphenicol marker

Depositor

Dan Mulvihill

Article

Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

Inserts

naa10 (naa10 Fission Yeast, Synthetic)
naa15 (naa15 Fission Yeast)

Use

Tags

Expression

Bacterial

Mutation

Codon optomised for E.coli expression

Promoter

T7

Available since

Feb. 8, 2016

Availability

Academic Institutions and Nonprofits only

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MDO1-c014

Plasmid

#173103

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

MACROD1 (MACROD1 Human)

Use

Tags

mCerulean

Expression

Bacterial

Mutation

S58-A325, codon optimized

Promoter

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-131

Plasmid

#118517

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mTurquoise2

Use

Minitn5 delivery vector

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-HA-ERK2 R67S

Plasmid

#53201

Purpose

Expresses GST and HA tagged human ERK2 R67S from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK2 (MAPK1 Human)

Use

Tags

GST and HA

Expression

Bacterial

Mutation

R67S

Promoter

Available since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
pHS0570 pET45b(+) T7/LacO His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep

Plasmid

#176534

Purpose

Bacterial expression of His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

CRISPR-associated IscB (Delaware Bay metagenome)

Use

Tags

14xHis-MBP-bdSUMO and TwinStrep

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pTarget: Ptaq-RFP_PlacIq-GFP

Plasmid

#192280

Purpose

RFP expressed under constitutive promoter Ptaq and GFP expressed under constitutive promoter Placiq

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Inserts

RFP
GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PlacIq and Ptaq

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-mCherry-hPMCA2x/b

Plasmid

#111571

Purpose

Expresses a plasma membrane calcium pump hPMCA2x/b

Depositor

Baljit Khakh

Article

Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

Insert

Plasma membrane calcium pump 2, variant x/b (ATP2B2 Human)

Use

Tags

mCherry

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pEF019 MBP-β-catenin-His

Plasmid

#154070

Purpose

Expresses MBP-β-catenin-His (human β-catenin as a fusion protein with MBP and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

CTNNB1 (CTNNB1 Human)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

Tac

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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EPB104

Plasmid

#68369

Purpose

contains human U6 promoter

Depositor

Eric-Paul Bennett

Article

Lonowski et al Nat Protoc. 2017 Mar;12(3):581-603. doi:

Insert

U6 promoter (RNU6-1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

U6

Available since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pBbE6c-RFP

Plasmid

#35287

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

LlacO-1

Available since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

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GFP-(SUMO)1

Plasmid

#127089

Purpose

Bacterial expression plasmid for eGFP-(SUMO)1 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

EGFP human SUMO3 (SUMO3 Human)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

unknown

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST17-TARG1

Plasmid

#172594

Purpose

For purification (from E.coli) of His-tagged TARG1

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

TARG1 (OARD1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCri_L830-antiSH2-OptoMB-V416L

Plasmid

#159936

Purpose

Opto-MB bacterial expression

Depositor

Jose Avalos, Jared Toettcher

Article

Carrasco-Lopez et al Nat Commun. 2020 Aug 13;11(1):4045. doi:

Insert

anti-SH2 MB with SS58 insert

Use

Tags

Expression

Bacterial

Mutation

AsLOV2 V416L

Promoter

Available since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCri_L889-antiSH2-OptoMB-Triple

Plasmid

#159937

Purpose

Opto-MB bacterial expression

Depositor

Jose Avalos, Jared Toettcher

Article

Carrasco-Lopez et al Nat Commun. 2020 Aug 13;11(1):4045. doi:

Insert

anti-SH2 MB with SS58 insert

Use

Tags

Expression

Bacterial

Mutation

AsLOV2 V416I_G528A_N538E

Promoter

Available since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pTAMAHISTEV_PfeAQ482A

Plasmid

#128945

Purpose

expresses Q482A mutant of PfeA in E. coli

Depositor

James Naismith

Article

Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

Insert

ferric enterobactin receptor

Use

Tags

TEV protease cleavable 7xHis and TamA

Expression

Bacterial

Mutation

Signal peptide sequence has been removed (1-75), …

Promoter

T7

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pHL 1075

Plasmid

#53004

Purpose

pCon:fhlA::mCherry, pLlacO-1:sodB::gfp

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Inserts

fhlA
sodB

Use

Synthetic Biology

Tags

GFP and mCherry

Expression

Bacterial

Mutation

contains −309 to +96 bp relative to start codon a…

Promoter

pCon and pLlacO-1

Available since

May 16, 2014

Availability

Academic Institutions and Nonprofits only
pKD279.8

Plasmid

#173171

Purpose

Encodes expression of SO_4388(REC)-PsdR(DBD) 137 and an sfGFP reporter

Depositor

Jeffrey Tabor

Article

Cartwright et al Proc Natl Acad Sci U S A. 2021 May 18;11

Inserts

SO_4388(REC)-PsdR(DBD) 137
sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23108 and PpsdA110

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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ARH2-c003

Plasmid

#173094

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

ADPRHL1 (ADPRHL1 Human)

Use

Tags

mCerulean

Expression

Bacterial

Mutation

E2-K354, codon optimized

Promoter

Available since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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