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pCDF_AaRaf1/RbcX2/BSD2/RbcX1

Plasmid

#229513

Purpose

Expresses Anthoceros agrestis chaperones: Raf1,RbcX2,BSD2,RbcX1

Depositor

Laura Gunn

Article

Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.

Inserts

Rubisco accumulation factor 1
RbcX2
BSD2
RbcX1

Expression

Bacterial

Mutation

N terminus chloroplast transit peptide truncation

Promoter

T7

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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pTW335m

Plasmid

#237328

Purpose

expresses M. truncatula RbcL RbcS-6xHis

Depositor

Tina Wang

Article

Archer et al J Exp Bot. 2025 Mar 31:eraf140. doi: 10.

Inserts

M. truncatula RbcL
M. truncatula RbcS-6xHis

Tags

6xHis and None

Expression

Bacterial

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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M-NylC_pET28a

Plasmid

#215409

Purpose

Expression construct for M-NylC gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

M-NylC

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pTXB1-TnY

Plasmid

#163975

Purpose

IPTG-inducible expression of Transposon TnY fused to Mxe Intein and Chitin-binding domain.

Depositor

Peter Smibert

Article

Liscovitch-Brauer et al Nat Biotechnol. 2021 Apr 29. pii: 10.103

Insert

TnY

Tags

Mxe intein - Chitin-binding domain

Expression

Bacterial

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX Grb2 SH2-SH3

Plasmid

#46442

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Grb2 (GRB2 Human)

Tags

GST and Thrombin site

Expression

Bacterial

Mutation

contains amino acids 1-217

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)_NAD-Snifit

Plasmid

#113658

Purpose

Plasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring NAD+ concentration

Depositor

Kai Johnsson

Article

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Insert

NAD-Snifit

Expression

Bacterial

Promoter

T7

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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MBP-FUS_FL_12E

Plasmid

#98652

Purpose

Expresses MBP-tagged full length phosphomimetic FUS

Depositor

Nicolas Fawzi

Article

Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

Insert

FUS (FUS Human)

Tags

6xHis-MBP

Expression

Bacterial

Mutation

12 S/T to E

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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Drp1-EGFP-StrepII

Plasmid

#174429

Purpose

Expresses human Drp1 Isoform 3 with EGFP at C-terminus in bacteria

Depositor

Thomas Pucadyil

Article

Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

Insert

Drp1

Tags

EGFP-StrepII

Expression

Bacterial

Mutation

None

Promoter

T7

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pAIP1

Plasmid

#233685

Purpose

Engineered SUMO protease expression plasmid designed for efficient protease-mediated elution.

Depositor

Christopher Bahl

Insert

Ulp1-like SUMO protease

Tags

CAHS-107838 and HisTag

Expression

Bacterial

Promoter

T7

Available Since

March 4, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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GST-mCh-FYVE

Plasmid

#192289

Purpose

Expresses GST- and mCherry-tagged HRS FYVE in e Coli

Depositor

James Hurley

Article

Jensen et al Sci Adv. 2022 Dec 14;8(50):eadd1436. doi

Insert

HRS FYVE domain

Tags

GST-TEV-mCherry

Expression

Bacterial

Promoter

tac

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pET21b-P35

Plasmid

#11808

Depositor

Guy Salvesen

Article

Riedl et al Biochemistry. 2001 Nov 6. 40(44):13274-8

Insert

P35

Expression

Bacterial

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
PdnaK GFP pZa

Plasmid

#170073

Purpose

GFP expression under the control of E. coli dnaK promoter

Depositor

Urartu Seker

Article

Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

Insert

Green fluorescent protein

Use

Synthetic Biology

Expression

Bacterial

Promoter

PdnaK

Available Since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX Emt-SH2

Plasmid

#46424

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Emt (ITK Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 223-346

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pRB035

Plasmid

#181944

Purpose

aTc-inducible expression of mNeonGreen

Depositor

Jeffrey Tabor

Article

Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

Insert

mNeonGreen; tetR (AX04_RS23485 Synthetic)

Expression

Bacterial

Promoter

mNG-PLtetO-1; tetR-J23106

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pRAV24

Plasmid

#67716

Purpose

Parental plasmid for transcription of RNAs with a GlmS/M-domain 3'-tag for native purification

Depositor

Robert Batey, Jeffrey Kieft

Article

Batey et al RNA. 2007 Aug;13(8):1384-9. Epub 2007 Ju

Insert

Transcription cassette for 3'-tagged RNAs

Expression

Bacterial

Promoter

T7 RNA polymerase

Available Since

Sept. 8, 2015

Availability

Academic Institutions and Nonprofits only
pSb_init containing anti SARS-CoV-2_RBD sybody Sb#45

Plasmid

#153526

Purpose

Expression of the anti SARS-CoV-2_RBD sybody Sb#45

Depositor

Markus Seeger

Article

Walter et al bioRxiv 2020.04.16.045419

Insert

Sb#45

Expression

Bacterial

Promoter

pBAD

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pET19-M(BRCT)TdTwt

Plasmid

#207459

Purpose

Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) variant, including the N-terminal BRCT domain

Depositor

Jay Keasling

Article

Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

Insert

M(BRCT)TdTwt (Dntt Mouse)

Tags

10xHis-MBP

Expression

Bacterial

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-MinE-His

Plasmid

#133623

Purpose

Expression vector for the Escherichia coli MinE with an C-terminal fusion of a His-tag for purification.

Depositor

Petra Schwille

Article

Glock et al ACS Synth Biol. 2018 Dec 20. doi: 10.102

Insert

MinE-His (minE E. coli)

Tags

His

Expression

Bacterial

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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p0857 pBR322 SV40

Plasmid

#10877

Depositor

Peter Howley

Insert

SV40

Expression

Bacterial

Available Since

Oct. 26, 2005

Availability

Academic Institutions and Nonprofits only
pGex-Cav1

Plasmid

#33364

Depositor

Ambra Pozzi

Article

Borza et al J Biol Chem. 2010 Dec 17;285(51):40114-2

Insert

Cav1 (CAV1 Human)

Tags

GST

Expression

Bacterial

Available Since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only