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pET30a-Spike-E

Plasmid

#165096

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 spike protein (S SARS-CoV2)

Expression

Bacterial

Available Since

Feb. 11, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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bdSUMO-HA2-GFP-HiBit

Plasmid

#171017

Purpose

For bacterial expression of bdSUMO tagged muGFP with HA2 peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-HA2-muGFP-HiBiT

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pEQ111m

Plasmid

#40190

Depositor

Paul Modrich

Article

Wright et al J Biol Chem. 1989 Jul 15;264(20):11816-2

Insert

EcoRI endonuclease & EcoRI methylase

Expression

Bacterial

Mutation

changed Glutamic Acid 111 to Glutamine

Promoter

Lambda PL

Available Since

Nov. 19, 2012

Availability

Academic Institutions and Nonprofits only
HS-Par3-GFP

Plasmid

#105947

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

Par3

Tags

GFP

Expression

Bacterial

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pH6HTC_YBX3-Halo

Plasmid

#175308

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

YBX3 (YBX3 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pSB208

Plasmid

#164123

Purpose

Ptac driving PP7 repeats

Depositor

Christopher Voigt

Article

Shao et al Nat Commun. 2021 Mar 5;12(1):1475. doi:

Insert

YFP-PP7

Expression

Bacterial

Promoter

Ptac

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pL-RPB1

Plasmid

#10981

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

RPB1 (RPO21 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Wild type RPB1 gene

Available Since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only

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YL-CashGem-BAT1-URA3-LINEAR

Plasmid

#174837

Purpose

pRS414 backbone containing LINEAR fragment targeting BAT1 in Y. lipolytica

Depositor

Zengyi Shao

Article

Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

Insert

Codon optimized Cas9:hGem

Use

CRISPR and Synthetic Biology

Tags

hGeminin tag

Expression

Bacterial and Yeast

Promoter

GPDp

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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B23(9-122)

Plasmid

#23142

Depositor

SeWon Suh

Article

Lee et al Proteins. 2007 Nov 15. 69(3):672-8.

Insert

B23 core (NPM1 Human)

Tags

His

Expression

Bacterial

Mutation

deleted amino acids 1-8

Available Since

Sept. 16, 2010

Availability

Academic Institutions and Nonprofits only
GST TopBP1 (aa 32-1522) His

Plasmid

#20375

Depositor

Aziz Sancar

Article

Choi et al Nucleic Acids Res. 2009 Apr;37(5):1501-9

Insert

TopBP1 (TOPBP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Starts at aa32

Available Since

March 5, 2009

Availability

Academic Institutions and Nonprofits only
pBHM2619

Plasmid

#234498

Purpose

amdS-CnU6-empty sgRNA

Depositor

Hiten Madhani

Article

Nalley et al G3 (Bethesda). 2025 Aug 6;15(8):jkaf118.

Insert

amdS-PCnU6-sgRNA_scaffold

Use

CRISPR

Expression

Bacterial and Yeast

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pBHM2662

Plasmid

#234501

Purpose

Linker-Cno_mNeonGreen-CBP-2xFLAG-CnU6-sgRNA-amdS

Depositor

Hiten Madhani

Article

Nalley et al G3 (Bethesda). 2025 Aug 6;15(8):jkaf118.

Insert

mNeonGreen-CBP-2XFLAG-amdS-PCnU6-sgRNA_scaffold

Use

CRISPR

Expression

Bacterial and Yeast

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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PCMV-Tag4A-MMP8 (E138Q)

Plasmid

#29549

Depositor

Yardena Samuels

Article

Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

Insert

Metalloproteinase protein 8 (MMP8 Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

E138Q

Available Since

July 22, 2011

Availability

Academic Institutions and Nonprofits only
pET24b-BG1.3

Plasmid

#199437

Purpose

expresses BAmGreen1.3 in bacterial cells

Depositor

Andrew Woolley

Article

Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

Insert

BAMGreen1.3

Tags

his tag

Expression

Bacterial

Promoter

T7

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR-attL5-CRY2-mCh-attL2

Plasmid

#160438

Purpose

Entry vector for cloning Cryptochrome2-mCherry using 2-fragment gateway recombination

Depositor

Nicholas Tolwinski

Article

Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

Insert

Cryptochrome 2 (CRY2 Mustard Weed)

Tags

mCherry

Expression

Bacterial

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX6P1 p37

Plasmid

#169015

Purpose

Expresses GST-p37 (human, codon optimized for expression in bacteria)

Depositor

Hemmo Meyer

Article

Kracht et al J Mol Biol. 2020 Nov 20;432(23):6061-607

Insert

p37 (UBXN2B Human)

Tags

GST

Expression

Bacterial

Mutation

sequence was codon optimized for expression in E.…

Promoter

tac

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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pSPORT1[attB/Ins1/NbT promoter/glGFP/bglobin 3'-UTR/Ins2]

Plasmid

#74102

Purpose

plasmid driven under neuronal beta-tubulin promoter, bearing attB and two insulator sequences (opposite directions pointing inward towards the reporter gene)

Depositor

Ben Szaro

Article

Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

Inserts

Xenopus laevis beta-tubulin gene promoter region and 5'UTR
Green Lantern Green Fluorescent Protein
Rabbit beta 1-globin gene 3'UTR

Expression

Bacterial

Available Since

May 26, 2016

Availability

Academic Institutions and Nonprofits only

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pIB170

Plasmid

#90193

Purpose

E. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pveg promoter

Depositor

Indranil Biswas

Article

Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

Type

Empty backbone

Tags

None

Expression

Bacterial

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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MiCy-pBAD

Plasmid

#54565

Purpose

Localization: Protein Expression Vector , Excitation: 472, Emission: 495

Depositor

Michael Davidson, Atsushi Miyawaki

Article

Karasawa et al Biochem J. 2004 Jul 1. 381(Pt 1):307-12.

Type

Empty backbone

Tags

6xHis and MiCy

Expression

Bacterial

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only
pSPAsp-hp

Plasmid

#48122

Purpose

Shuttle vector E. coli/B.meg.; encoding the predicted optimal signal peptide for protein secretion using Bacillus megaterium under control of the xylose inducible promoter

Depositor

Dieter Jahn

Article

Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):

Insert

predicted optimal signal peptide for Bacillus megaterium protein secretion

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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