31,812 results
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Plasmid#84652PurposeE. coli expression vector derived from pET32 with Gateway cassette.DepositorTypeEmpty backboneTagsHis, S tag, and Trx TagExpressionBacterialPromoterT7Available SinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only
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PET-Sniper SpCas9-NLS-6xHis
Plasmid#207374PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertSniper SpCas9
UseCRISPRTags6xHisExpressionBacterialMutationF539S, M763I, K890NPromoterT7Available SinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
FHL-cpmTq2-Calcium-lifetime-sensor
Plasmid#129628PurposeGenetically encoded calcium sensor (GECI) that reports with lifetime and intensity changesDepositorInsertFHL-cpmTq2-Calcium-lifetime-sensor
Tags6x His - Modified TorA MNNNDLFQASRRRFLAQLG[G to …ExpressionBacterial and MammalianPromoterCMV,&RhamnoseAvailable SinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-(pos16)GFP
Plasmid#89249PurposeExpresses GFP with +16 theoretical charge in E.ColiDepositorInsertHis6-(pos16)-GFP
TagsHis6ExpressionBacterialPromoterT7Available SinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-His-Dre
Plasmid#123130PurposeBacterial expression plasmid for wild-type Dre with an N-terminal His-tag.DepositorInsertDre recombinase
Tags6x His tagExpressionBacterialPromoterT7Available SinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
His SUMO TEV WT SPOP 28-359
Plasmid#194115PurposeWild type His-SUMO SPOP expression plasmidDepositorAvailable SinceFeb. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSETB-roGFP1-R12
Plasmid#83311PurposeInducible expression of redox sensitive GFP in bacteriaDepositorInsertroGFP1-R12
Tags6xHISExpressionBacterialMutationC48S/Q80R/S147C/Q204C/N149K/F223R/S202KPromoterT7Available SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBSV2_P0031-mCherryBb
Plasmid#118240PurposeKanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes bb0031 promoter driven mCherryDepositorInsertbb0031 promoter driven mCherry
ExpressionBacterialAvailable SinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMB413a
Plasmid#20058DepositorInsertSperm Whale Myoglobin
ExpressionBacterialAvailable SinceJan. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGEX-DAXX-D/E-His
Plasmid#179619PurposeExpress DAXX D/E region in bacteriaDepositorInsertdeath domain associated protein (DAXX Human)
TagsGST, His, and TEVExpressionBacterialMutationamino acids 414-505 of DAXXAvailable SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLz37_CB_operon_CsoS2_N1-N4
Plasmid#140856PurposeHalothiobacillus neapolitanus carboxysome operon with full length CsoS2. For heterologous expression of carboxysomes in E. coli.DepositorInsertCbbL, CbbS, CsoS2 (full-length), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
ExpressionBacterialAvailable SinceAug. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_H-Luc
Plasmid#113924PurposePlasmid encoding the bacterial expression of the chimeric HaloTag7-NanoLucCP fusion dubbed H-Luc, for BRETDepositorInsertH-Luc
UseLuciferaseExpressionBacterialPromoterT7Available SinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKD233.7-3
Plasmid#90955PurposeExpresses S. baltica TtrR under the PltetO-1 promoter, sfGFP under PttrB185-269, and mCherry constitutivelyDepositorInsertsTetrathionate response regulator
Superfolder GFP
mCherry
ExpressionBacterialPromoterBba_J23114, PltetO-1, and PttrB185-269Available SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDx_mScarlet3-NCwt-SYFP2
Plasmid#189766PurposeDual expression vector for bacteria and mammalian cells producing mScarlet3-NCwt-SYFP2 fusion protein for FRET analysisDepositorInsertmScarlet3-NCwt-SYFP2
Tags6xHisExpressionBacterial and MammalianMutationencodes the expected internal mutations relative …PromoterCMV & RhaAvailable SinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHL 1032
Plasmid#52997PurposepLtetO-1:RhyB, pCon:ompC::mCherry, pLlacO-1:sodB::gfpDepositorInsertsRhyB sRNA
ompC
sodB
UseSynthetic BiologyTagsgfp and mCherryExpressionBacterialMutationcontains −56 to +141 bp relative to start codon a…PromoterpCon, pLlacO-1, and pLtetO-1Available SinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEaaK-NanR-J23113-pNanA-CadC-pCadBA-GFP
Plasmid#200277PurposeCharacterisation of the sialic acid-responsive circuit with fluorescent reporterDepositorInsertsCadC
NanR
ExpressionBacterialAvailable SinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNCS-mCardinal2
Plasmid#52631Purposebacterial expression vector with far-red FP mCardinal2DepositorInsertmCardinal2
ExpressionBacterialAvailable SinceApril 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJA48 HLA-B*57:01
Plasmid#180444PurposeE. coli expression of soluble MHC proteinDepositorInsertHLA-B*57:01
TagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…Available SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc1-HISNDHFR
Plasmid#63940PurposePosition 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneTags6xHis; TEV cleavableExpressionBacterialPromoterT7Available SinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSL0828 (pQCascade_crRNA-4)
Plasmid#130637PurposeExpresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a guide RNA that targets lacZ.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(target-4)
UseCRISPR; TransposonExpressionBacterialPromoterT7Available SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only