30,307 results

1. pBbE7k-RFP

   Plasmid

   #35315

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   RFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pT2/SVNeo

   Plasmid

   #26553

   Depositor

   Perry Hackett

   Article

   Cui et al J Mol Biol. 2002 May 17. 318(5):1221-35.

   Insert

   pT2/SVNeo
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   added the Rmut(80, 87, d101)

   Promoter

   Available since

   March 14, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT7-L1T1L-NLuc

   Plasmid

   #128942

   Purpose

   Expresses L1-NLuc gene

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al J Virol. 2019 May 8. pii: JVI.00401-19.

   Insert

   L1 minor core protein
   

   Use

   Tags

   Nanoluc

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promotor

   Available since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSMT3-FBF2

   Plasmid

   #166972

   Purpose

   Expresses FBF-2 in E. coli for purification and crystallization studies

   Depositor

   Traci Hall

   Article

   Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1

   Insert

   FBF-2 (fbf-2 Nematode)
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   FBF-2 PUM RNA binding domain; amino acids 164-575

   Promoter

   T7

   Available since

   April 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOPINVH

   Plasmid

   #26041

   Depositor

   Ray Owens

   Article

   Nettleship et al Protein Expr Purif. 2008 Nov . 62(1):83-

   Insert

   u-LacZ-KHHHHHH
   

   Use

   Tags

   KHHHHHH and u-phosphatase secretion leader

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   June 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pH3U3-zif268 (omega)

   Plasmid

   #18046

   Depositor

   Scot Wolfe

   Article

   Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

   Insert

   zif268 binding site
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   The preferred binding site for zif268 is inserted…

   Promoter

   Available since

   June 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. DUSP13

   Plasmid

   #38824

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   DUSP13 (Dusp13 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

8. NXR1A

   Plasmid

   #42430

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   NXR1A (NUDT18 Human)
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   See Comments

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pDule-Mb haloTyrRS C6

   Plasmid

   #160377

   Purpose

   C6 HaloTyrosine tRNA synthatase and cognate amber suppressing tRNA derived from M. barkeri.

   Depositor

   Ryan Mehl

   Article

   Jang et al ACS Chem Biol. 2020 Feb 21;15(2):562-574

   Insert

   C6 HaloTyrosine tRNA synthatase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   L270S, Y271L, N311G, C313T

   Promoter

   Available since

   Nov. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pH6HTC_HNRNPA1-Halo

    Plasmid

    #175307

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    HNRNPA1 (HNRNPA1 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTE-28S15-0X

    Plasmid

    #20319

    Depositor

    Dirk Schnappinger

    Article

    Klotzsche et al Nucleic Acids Res. 2009 Apr;37(6):1778-8

    Insert

    Psmyc-tetR#28
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAC1749

    Plasmid

    #127983

    Purpose

    AMA1 plasmid with Aspergillus optimized Cpf1, hygB selection marker, and USER cassette (PacI/Nt.BbvCI)

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

    Insert

    Cpf1
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    codon optimized for Aspergillus nidulans

    Promoter

    Aspergillus nidulans tef1 promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC-UAS1B16-TEF(504)

    Plasmid

    #44371

    Depositor

    Hal Alper

    Article

    Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

    Insert

    UAS1B16-TEF(504) promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    UAS1B16-TEF(504)

    Available since

    July 15, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pGBW-m4046852

    Plasmid

    #145584

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp8

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp8 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pENTR1A-SynTetOff-GFP

    Plasmid

    #190230

    Purpose

    Gateway entry vector, high-level expression of EGFP in neuronal cells

    Depositor

    Hiroyuki Hioki

    Article

    Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBWB419

    Plasmid

    #140637

    Purpose

    RSF1010-Cas9-pcaHG gRNA. Vector with constitutive Cas9 with gRNA targeting pcaHG locus.

    Depositor

    Keith Tyo

    Article

    Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFLxR5

    Plasmid

    #149482

    Purpose

    Broad-Host range expression vector, RSF1010 origin, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.

    Depositor

    Christopher Reisch

    Article

    Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

    Insert

    LuxR-mRFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLuxB

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GSK3beta/pET29b

    Plasmid

    #16315

    Depositor

    Peter Klein

    Article

    Hedgepeth et al Dev Biol. 1997 May 1. 185(1):82-91.

    Insert

    GSK3beta (gsk3b.L Frog)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pET26b_Nb.AT110.i1

    Plasmid

    #135481

    Purpose

    E. coli periplasmic expression of high-affinity conformational nanobody Nb.AT110.i1 recognizing angiotensin II type 1 receptor

    Depositor

    Andrew Kruse

    Article

    Wingler et al Cell. 2019 Jan 24;176(3):479-490.e12. do

    Insert

    Nanobody AT110.i1
    

    Use

    Tags

    6xHis-tag and pelB leader

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET LIC cloning vector with BioBrick polycistronic restriction sites (9A)

    Plasmid

    #48283

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge