30,808 results
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Plasmid#117701PurposeExpression of WT mouse SOD1 with N- terminal His tagDepositorAvailable SinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only
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pCPP3416
Plasmid#128720PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopT1-1
ExpressionBacterialAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5534
Plasmid#128723PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopX1
ExpressionBacterialAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTWIN1-His6-Ssp-Httex1-43Q
Plasmid#84352PurposeExpression of the human Huntingtin Exon1 protein containing 43Q in E.coliDepositorInsertHuntingtin Exon 1 (HTT Human)
TagsN-terminal Ssp intein (His-tagged)ExpressionBacterialPromoterT7Available SinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
ELK1_pet28a
Plasmid#131647PurposeBacterial expression of ETS gene ELK1DepositorAvailable SinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUNIV-EGFP
Plasmid#24706DepositorInsertEGFP (eGFP Aequoria victoria)
ExpressionBacterial, Mammalian, and Y…Available SinceDec. 6, 2010AvailabilityAcademic Institutions and Nonprofits only -
pKSJ331
Plasmid#103063PurposeExpresses colicin E1 and E1 immunity protein without E1 lysis (kil) geneDepositorInsertColicin E1 and E1 immunity protein
ExpressionBacterialPromotercolicin promotor from ColE1Available SinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFTC1
Plasmid#64951Purposesource of TcR FRT cassetteDepositorInsertFRT-TcR-FRT
ExpressionBacterialAvailable SinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLC133
Plasmid#198639PurposeExpression plasmid for dCas9 in E. coli used in Chip-seq experiments. dCas9 is tagged with a C-terminal 3x FLAG tag. A guide RNA can be cloned using BsaI restriction sites.DepositorInsertdCas9
Tags3x-FLAGExpressionBacterialPromoterpTetAvailable SinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJS183
Plasmid#134142PurposeBacterial expression of GFP-Ska1 and Ska2DepositorInsertHis-GFP-human Ska1; human Ska2
TagsGFPExpressionBacterialAvailable SinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDOC-K-glmS-GFPfor
Plasmid#158059PurposeDonor plasmid for insertion of eGFP and the constitutive acpP promoter into the S. Typhimurium chromosome. GFP is in the forward orientation relative to the kanamycin resistance selectable markerDepositorInsertGreen Fluorescent Protein optimised for excitation with UV light
UseSynthetic BiologyExpressionBacterialPromoteracpPAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJBL701
Plasmid#140371PurposeExpresses TetR protein under the control of T7-lacO promoterDepositorInsertT7-lacO-TetR-T
Tags6XHisExpressionBacterialAvailable SinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZP46 Prickle-2
Plasmid#16896DepositorInsertPrickle-2 (prickle2b Zebrafish)
ExpressionBacterialAvailable SinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFP.R377
Plasmid#138247PurposeExpresses the split mCherry reporter interrupted by the Mja-KlbA intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
Kindlin
Plasmid#186135PurposeFor expression of recombinant kindlin in bacteriaDepositorAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETNb-nALFA
Plasmid#159988PurposeFor the protein expression with ALFA tag at N-terminus in E. coliDepositorTypeEmpty backboneTagsMVKYI-8histidine-ALFA tag-HRV3C protease cleavage…ExpressionBacterialAvailable SinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET24a MDH_9CREN
Plasmid#204243PurposeBacterial expression of I. islandicus malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDH_9CREN
Tags6X HisExpressionBacterialPromoterLacAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQ-Elo-HB-C-A(1-630)
Plasmid#171086PurposeCo-expresses of ΔC-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOBDepositorTagsHisExpressionBacterialMutationDeleted N-term 26aa and C-term 142aa for mutant e…PromoterT5 promoterAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-Zdk1-TIAM1 DH/PH
Plasmid#81129Purposemammalian expression of Zdk1 tagged Tiam1 Dh/PHDepositorAvailable SinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSET-dCas9-actII-4-NT-S1
Plasmid#110185PurposeRepression of gene expression in Streptomyces by CRISPRiDepositorInsertsdCas9
sgRNA
UseCRISPR; IExpressionBacterialMutationD10A, H840APromoterJ23119 and ermE*pAvailable SinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only