30,320 results

1. pRAF-MutS

   Plasmid

   #86605

   Purpose

   E. coli MutS expression plasmid

   Depositor

   Richard Fishel

   Article

   Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

   Insert

   E. coli MutS (D835R,R840E)
   

   Use

   Tags

   his-tag and ald-tag

   Expression

   Bacterial

   Mutation

   D835R, R840E

   Promoter

   T7

   Available since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC-UAS1B16-TEF(272)

   Plasmid

   #44375

   Depositor

   Hal Alper

   Article

   Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

   Insert

   UAS1B16-TEF(272) promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   UAS1B16-TEF(272)

   Available since

   April 18, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pTW170b2

   Plasmid

   #136929

   Purpose

   Directed evolution of SpCas9

   Depositor

   David Liu

   Article

   Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

   Insert

   gVI
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. NFAT5 luciferase

   Plasmid

   #14110

   Depositor

   Anjana Rao

   Article

   Jauliac et al Nat Cell Biol. 2002 Jul . 4(7):540-4.

   Insert

   NM_001113178.2 (NFAT5 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

5. PclpB riboswitch GFP pZa

   Plasmid

   #170078

   Purpose

   GFP expression under the control of E. coli clpB promoter engineered with riboregulators

   Depositor

   Urartu Seker

   Article

   Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

   Insert

   Green fluorescent protein
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PclpB

   Available since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBAD_mCherry

   Plasmid

   #187387

   Purpose

   arabinose-inducible expression of mCherry

   Depositor

   Roi Avraham

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBSM

   Plasmid

   #67505

   Purpose

   Express the tRNAMet scaffold

   Depositor

   Luc Ponchon

   Article

   Ponchon et al Nat Protoc. 2009;4(6):947-59. doi: 10.10

   Insert

   modified tRNAMet
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lpp

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pGEX4T1-Smurf2 wt

   Plasmid

   #13504

   Depositor

   Jeff Wrana

   Article

   Kavsak et al Mol Cell. 2000 Dec . 6(6):1365-75.

   Insert

   SMURF2 (SMURF2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 4, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pNO183

   Plasmid

   #101068

   Purpose

   ppsR2-bphP1 bicistronic operon plasmid

   Depositor

   Jeffrey Tabor

   Article

   Ong et al ACS Synth Biol. 2017 Nov 1. doi: 10.1021

   Inserts

   ppsR2 (ppsR2 Rhodopseudomonas palustris CGA009)
   bphP1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   insert guanine at position 430 to fix frameshift …

   Promoter

   Available since

   Nov. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMAL-BRT17

    Plasmid

    #45358

    Depositor

    Scott Banta

    Article

    Shur et al Biotechniques. 2013 Apr;54(4):197-206. d

    Insert

    17 Repeat Beta Roll Tag
    

    Use

    Tags

    Maltose Binding Protein (MBP)

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac

    Available since

    June 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. 6CCR (GID4)

    Plasmid

    #119960

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Insert

    GID4 (C17orf39 Human)
    

    Use

    Tags

    6xHIS, thrombin,SUMO and TEV cleavage

    Expression

    Bacterial

    Mutation

    contains amino acids 116-300

    Promoter

    T7

    Available since

    Jan. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pET-51b(+)-cpHalo∆

    Plasmid

    #194659

    Purpose

    Protein production of the split HaloTag part cpHalo∆ (cpHaloTag135/156) in bacteria

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    cpHalo∆
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Tensin-2 WT

    Plasmid

    #201797

    Purpose

    Expresses wild-type Tensin-2 protein in MAC-Tag-C backbone

    Depositor

    Vesa Hytönen

    Article

    Turkki et al Sci Rep. 2024 Aug 27;14(1):19862. doi: 1

    Insert

    Tensin-2 (Tns2 Mouse)
    

    Use

    Tags

    BirA, HA, strepIII

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTE5400_pMBP_CDS dropout vector

    Plasmid

    #221189

    Purpose

    Expression vector for N-terminally His-tagged maltose binding protein (10xHis-MBP) fused to a protein of interest

    Depositor

    Tobias Erb

    Article

    Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0

    Type

    Empty backbone

    Use

    Tags

    10x His-tag + E. coli maltose binding protein (MB…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJCC_083 Cas12i1

    Plasmid

    #171670

    Purpose

    For bacterial expression of Cas12i1 with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

    Insert

    Cas12i1
    

    Use

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFA-DAB2

    Plasmid

    #162690

    Purpose

    Expresses DabAB2 inorganic carbon transport complex

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    DabAB2 inorganic carbon transport complex
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLtet0-1 promoter

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. His-GIV-CTs

    Plasmid

    #69864

    Purpose

    Express His tagged GIV-CTs (1660–1870).

    Depositor

    Pradipta Ghosh

    Article

    Garcia-Marcos et al Mol Biol Cell. 2011 Mar 1;22(5):673-86.

    Insert

    GIV/Girdin (CCDC88A Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    C terminal amino acids S1660-S1870 of human GIV.

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJCC_039 FnCas12a

    Plasmid

    #171666

    Purpose

    For bacterial expression of FnCas12a with a C-terminal sorting motif and His tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

    Insert

    FnCas12a
    

    Use

    Tags

    6X His and sortase A sorting motif

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDDRFP-A1

    Plasmid

    #36289

    Depositor

    Robert Campbell

    Article

    Alford et al Chem Biol. 2012 Mar 23;19(3):353-60.

    Insert

    Dimerization dependent RFP A1
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 26, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pET151-hUCH37 (ISF1)

    Plasmid

    #61929

    Purpose

    Expression of hUCH37 (ISF1) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Insert

    hUCH37 (ISF1) (UCHL5 Human)
    

    Use

    Tags

    6x HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only