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MICU-APEX2

Plasmid

#79057

Purpose

A C-terminal APEX2 fusion to the human mitochondrial calcium uptake 1 protein MICU1

Depositor

Alice Ting

Publication

Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

Insert

C-terminal APEX2 fused MICU1 (MICU1 Soybean, Human)

Use

Lentiviral

Tags

FLAG

Expression

Bacterial and Mammalian

Promoter

CMV immearly promotor

Available since

Sept. 1, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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mCardinal-pBAD

Plasmid

#54800

Purpose

Localization: Protein Expression Vector , Excitation: 604, Emission: 659

Depositor

Michael Davidson

Publication

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Type

Empty backbone

Tags

6xHis and mCardinal

Expression

Bacterial

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pWUR702

Plasmid

#53079

Purpose

Inducible TtAgo expression

Depositor

John van der Oost

Publication

Swarts et al Nature. 2014 Mar 13;507(7491):258-61. do

Insert

TtAgo

Tags

Strep-II tag

Expression

Bacterial

Promoter

T7

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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pACYCT2

Plasmid

#45799

Depositor

Luc Ponchon

Publication

Ponchon et al Nucleic Acids Res. 2013 Aug;41(15):e150.

Type

Empty backbone

Expression

Bacterial

Available since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBAD/HisB-iRFP670

Plasmid

#45453

Depositor

Vladislav Verkhusha

Publication

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP670

Expression

Bacterial

Mutation

T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

Promoter

araBAD

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Pet28a_Synthetic_Human_H2A.1

Plasmid

#42634

Depositor

Joe Landry

Insert

Human H2A.1 (H2AC13 Human, Synthetic)

Tags

Untagged

Expression

Bacterial

Mutation

Codon Optimized

Promoter

T7

Available since

Feb. 25, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

6 Articles

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pET Biotin His6 GFP LIC cloning vector (H6-msfGFP)

Plasmid

#29725

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, AviTag (biotin), SiriusGFP, and TEV cleava…

Expression

Bacterial

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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TEVSH

Plasmid

#125194

Purpose

His-tagged TEV protease with solubility increasing mutations

Depositor

Helena Berglund

Publication

van den Berg et al J Biotechnol. 2006 Feb 10;121(3):291-8.

Insert

TEV_SH

Tags

V5 His-tag

Expression

Bacterial

Promoter

T7lac

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pQE30-His-CKM

Plasmid

#124134

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Publication

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Creatine kinase (CKM Chicken)

Tags

6x-His

Expression

Bacterial

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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PCV-Cas9

Plasmid

#123643

Purpose

Expresses Cas9 with an N-terminal HUH-tag called PCV2

Depositor

Wendy Gordon

Publication

Aird et al Commun Biol. 2018 May 31;1:54. doi: 10.1

Inserts

PCV2 (ORF1 )
Cas9

Tags

(EAAAK)8 linker and His6 + SUMO

Expression

Bacterial

Mutation

2-116

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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phage UbiC tagRFP-T-DDX6

Plasmid

#119947

Purpose

Lentiviral vector expressing tagRFP-T-DDX6 fusion protein

Depositor

Jeffrey Chao

Publication

Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

DDX6 (DDX6 Human)

Use

Lentiviral

Tags

tagRFP-T

Expression

Bacterial and Mammalian

Promoter

UbiC

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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LVXN-Neo-NSD2

Plasmid

#86010

Purpose

express wild-type NSD2 in mammalian cells

Depositor

Darrin Stuart

Publication

Jaffe et al Nat Genet. 2013 Nov;45(11):1386-91. doi:

Insert

NSD2 (NSD2 Human)

Use

Lentiviral

Tags

Flag tag D Y K D D D D K

Expression

Bacterial

Mutation

K1002R (please see depositor comments below)

Promoter

CMV

Available since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJMK001

Plasmid

#33780

Depositor

Adam Cohen

Publication

Kralj et al Science. 2011 Jul 15;333(6040):345-8.

Insert

Proteorhodopsin Optical Proton Sensor

Expression

Bacterial

Mutation

D97N

Promoter

AraBAD

Available since

Dec. 14, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pFNF

Plasmid

#22687

Depositor

Robert Benezra

Publication

Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

Insert

Neo*

Use

Mouse Targeting

Expression

Bacterial and Mammalian

Mutation

FRT-pPGK-pEM7-Neo*-bpA-FRT

Available since

Dec. 17, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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mRFP-human APPL1

Plasmid

#22202

Depositor

Pietro De Camilli

Publication

Erdmann et al Dev Cell. 2007 Sep . 13(3):377-90.

Insert

Adaptor protein containing PH domain, PTB domain and Leucine zipper motif (APPL1 Human)

Tags

mRFP

Expression

Bacterial and Mammalian

Available since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pEGFP-C3-hYAP2

Plasmid

#17844

Depositor

Marius Sudol

Publication

Basu et al Mol Cell. 2003 Jan . 11(1):11-23.

Insert

YAP (YAP1 Human)

Tags

EGFP

Expression

Bacterial and Mammalian

Available since

May 29, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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VR124 pETv2 10His-pG-Tn5 (E54K, L372P)

Plasmid

#198468

Purpose

E. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in T7 Express lysY/Iq protein expression strain.

Depositor

Viviana Risca

Publication

Soroczynski et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

Insert

10His-pG-Tn5 (E54K, L372P)

Tags

10His-pG

Expression

Bacterial

Mutation

E54K, L372P

Promoter

Consensus φ10 T7 Promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSecUAG-Evol2

Plasmid

#163148

Purpose

Expresses machinery necessary for selenocysteine incorporation at UAG codons

Depositor

Dieter Söll

Publication

Mukai et al Angew Chem Int Ed Engl. 2018 Jun 11;57(2

Inserts

allo-tRNA UTu2D
thioredoxin
Selenophosphate synthase
Selenocysteine synthase
Cysteine desulfurase

Expression

Bacterial

Mutation

Cysteine 32 switched to Selenocysteine (UAG), Cys…

Promoter

EM7, araBAD, native As SelD promoter, and native …

Available since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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HP4_M13

Plasmid

#120340

Purpose

Helper plasmid for production of phagemid ssDNA, based on m13mp18

Depositor

Hendrik Dietz

Publication

Praetorius et al Nature. 2017 Dec 6;552(7683):84-87. doi:

Insert

Coding part of the M13 phage genome

Expression

Bacterial

Promoter

native phage promoters

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET28a-AviTag-SnoopLigase

Plasmid

#105626

Purpose

Plasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with AviTag for biotinylation

Depositor

Mark Howarth

Publication

Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3

Insert

AviTag-SnoopLigase

Expression

Bacterial

Promoter

T7

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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