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Bacterial Expression

30,535 results

Showing: 641 - 660 of 30535 results

  1. pEHIShqprt

    Plasmid
    #92166
    Purpose
    Plasmid pEHIShqprt expressing the full-length of human quinolinate phosphoribosyltransferase (hQPRTase) was constrcucted by inserting BspHI/BamHI-fragment into NcoI/BamHI digested pEHISTEV vector
    Depositor
    James Naismith, Huanting Liu
    Article
    Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E
    Insert
    Quinolinate Phosphoribosyltransferase (QPRT Human)
    Use
    Tags
    TEV protease -cleavable N-terminal 6His
    Expression
    Bacterial
    Mutation
    changed Asp 2 to Asn to create BspHI site
    Promoter
    T7
    Available Since
    Aug. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    92166-plasmid-map-sequence-id-190226

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  3. pCasSA

    Plasmid
    #98211
    Purpose
    CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9
    Depositor
    Quanjiang Ji
    Article
    Chen et al J Am Chem Soc. 2017 Mar 15;139(10):3790-
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    July 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    98211-plasmid-map-sequence-id-485072

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  5. pT2/BH

    Plasmid
    #26556
    Depositor
    Perry Hackett
    Insert
    pT2/BH
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Oct. 25, 2010
    Availability
    Academic Institutions and Nonprofits only
    26556-plasmid-map-sequence-id-203999

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  7. pNBU2_repA1

    Plasmid
    #211910
    Purpose
    Replicative vector for protein expression in Bacteroidales with an aTc inducible promoter, terminator, and a barcoding site.
    Depositor
    Adam Arkin
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Feb. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    211910-plasmid-map-sequence-id-421620

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  8. LentiGuide Puro-P2A-EGFP

    Plasmid
    #137729
    Purpose
    For simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).
    Depositor
    Fredrik Wermeling
    Article
    Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Tags
    EGFP
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available Since
    Feb. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    137729-plasmid-map-sequence-id-272646

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  9. pET-pA-Hia5

    Plasmid
    #174372
    Purpose
    SEE DEPOSITOR COMMENTS BELOW. Plasmid for IPTG-inducible expression and purification of protein A-Hia5-6xHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)
    Depositor
    Aaron Straight
    Article
    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi
    Insert
    protein A - hia5
    Use
    Tags
    6xHis and Protein A
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Sept. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    174372-plasmid-map-sequence-id-341860

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  14. pBAD-GINKO1

    Plasmid
    #113111
    Purpose
    Bacterial expression of genetically encoded green fluorescent potassium ion indicator GINKO1
    Depositor
    Robert Campbell
    Article
    Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1
    Insert
    GINKO1
    Use
    Tags
    6-His Tag, T7 tag (gene 10 leader), and Xpress™ t…
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD
    Available Since
    Aug. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    113111-plasmid-map-sequence-id-216972

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  15. pExp-DsbC

    Plasmid
    #112564
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbC (leaderless thiol:disulfide interchange protein DsbC from E. coli) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8xHis-DsbC and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112564-plasmid-map-sequence-id-217214

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Showing: 641 - 660 of 30535 results